Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Surg. Nov 27, 2020; 12(11): 442-459
Published online Nov 27, 2020. doi: 10.4240/wjgs.v12.i11.442
Identification of key genes controlling cancer stem cell characteristics in gastric cancer
Chao Huang, Ce-Gui Hu, Zhi-Kun Ning, Jun Huang, Zheng-Ming Zhu
Chao Huang, Ce-Gui Hu, Zhi-Kun Ning, Jun Huang, Zheng-Ming Zhu, Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
Author contributions: Huang C and Hu CG designed the study and contributed equally to this work; Huang C, Hu CG, and Ning ZK collected the data; Huang C and Huang J analysed the data; Huang C wrote the manuscript with contribution from all authors; and all authors read and approved the final version of the paper.
Supported by the National Natural Science Foundation of China, No. 81560389; and Key Research and Development Program of Jiangxi Province, No. 20181BBG70015.
Institutional review board statement: No human and/or animal subjects were involved in this study.
Conflict-of-interest statement: The authors declare no competing financial interests.
Data sharing statement: RNA sequencing results of 373 tissues and 348 human gastric adenomas and adenocarcinoma samples were obtained from TCGA database (https://portal.gdc.cancer.gov). The RNA-seq results of 30 normal samples and 343 cancer samples were merged into a matrix file using a script in the Perl language (http://www.perl.org/). We then converted the Ensembl ID in the matrix file to the gene name using the Ensembl database (http://www.ensembl.org/index.html) and the Perl language script. In addition, 406 pieces of clinical data were downloaded, and the relevant clinical data were collated and extracted using scripts in the Perl language. The calculation of mRNAsi was performed from the molecular spectrum of normal cells with different degrees of stemness.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Zheng-Ming Zhu, MD, Chairman, Surgical Oncologist, Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nanchang University, No. 1 Minde Road, Nanchang 330006, Jiangxi Province, China. zzm8654@163.com
Received: July 28, 2020
Peer-review started: July 28, 2020
First decision: August 9, 2020
Revised: August 13, 2020
Accepted: October 12, 2020
Article in press: October 12, 2020
Published online: November 27, 2020
Core Tip

Core Tip: This study found that the 16 key genes identified play an important role in the maintenance of gastric cancer stem cells. In addition, RAD54L, TPX2, and XRCC2 are most likely to be therapeutic targets for inhibiting the stemness characteristics of gastric cancer, providing a new idea for the treatment of gastric cancer.