Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Surg. Jul 27, 2023; 15(7): 1304-1316
Published online Jul 27, 2023. doi: 10.4240/wjgs.v15.i7.1304
Differences in metabolic improvement after metabolic surgery are linked to the gut microbiota in non-obese diabetic rats
Xin Luo, Cai Tan, Fang Tao, Chi-Ying Xu, Zhi-Hua Zheng, Qiang Pang, Xiang-An He, Jia-Qing Cao, Jin-Yuan Duan
Xin Luo, Fang Tao, Chi-Ying Xu, Jin-Yuan Duan, Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China
Cai Tan, Department of Women’s Health, Jiangxi Maternal and Child Health Hospital, Nanchang 330006, Jiangxi Province, China
Zhi-Hua Zheng, Qiang Pang, Xiang-An He, Jia-Qing Cao, Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China
Author contributions: Luo X and Tan C are co-first authors who have made significant contributions to this article; Luo X, Tan C, Cao JQ and Duan JY contributed to the conception of the study; Luo X, Tan C, Zheng ZH and Qiang P performed the experiment; Luo X, Tan C and Duan JY contributed significantly to analysis and manuscript preparation; Luo X, Tao F, Xue CY, Zheng ZH, Qiang P, He XA, Cao JQ and Duan JY helped perform the analysis with constructive discussions; Luo X, Tan C and Duan JY performed the data analyses and wrote the manuscript; All authors read and approved the final manuscript.
Supported by the National Natural Science Foundation of China, No. 81760156, 81960154 and 82060161; and the Natural Science Foundation of Jiangxi Province, No. 2018ACB21040, 20203BBGL73185, and 20212BAB206020; and Foundation of Health commission of Jiangxi Province, No. SKJP220225830.
Institutional animal care and use committee statement: The animal experiments were conducted according to the Nanchang University Guide to Animal Experiments.
Conflict-of-interest statement: All authors declare that there are no potential conflicts of interest.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at duanjy2022@outlook.com.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jin-Yuan Duan, Doctor, Department of General Surgery, The First Affiliated Hospital of Nanchang University, No. 1519 Dongyue Avenue, Nanchang 330006, Jiangxi Province, China. duanjy2022@outlook.com
Received: January 30, 2023
Peer-review started: January 30, 2023
First decision: March 24, 2023
Revised: April 6, 2023
Accepted: April 21, 2023
Article in press: April 21, 2023
Published online: July 27, 2023
Abstract
BACKGROUND

Different metabolic/bariatric surgery approaches vary in their effect on weight loss and glucose levels, although the underlying mechanism is unclear. Studies have demonstrated that the gut microbiota might be an important mechanism of improved metabolism after metabolic/bariatric surgery.

AIM

To investigate the relationship between the improvement in metabolic disturbances and the changes in gut microbiota after gastric or intestinal bypass.

METHODS

We performed sleeve gastrectomy (SG), distal small intestine bypass (DSIB) or sham surgery in nonobese rats with diabetes induced by 60 mg/kg streptozotocin (STZ-DM).

RESULTS

The group comparisons revealed that both SG and DSIB induced a reduction in body weight and significant improvements in glucose and lipid metabolism in the STZ-DM rats. Furthermore, DSIB exhibited a stronger glucose-lowering and lipid-reducing effect on STZ-DM rats than SG. 16S ribosomal RNA gene sequencing revealed the gut abundance of some Lactobacillus spp. increased in both the SG and DSIB groups after surgery. However, the DSIB group exhibited a more pronounced increase in the gut abundance of Lactobacillus spp. compared to the SG group, with more Lactobacillus spp. types increased in the gut.

CONCLUSION

The gut abundance of Lactobacillus was significantly correlated with the improvement in glycolipid metabolism and the change in serum fibroblast growth factor 21 levels.

Keywords: Diabetes, Glucose control, Lipid metabolism, Microbiota, Obesity, Gastrectomy, Distal small intestine bypass

Core Tip: Sleeve gastrectomy and distal small intestine bypass induced a reduction in body weight and significant improvements in glucose and lipid metabolism in the rats with streptozotocin-induced nonobese diabetes. The gut abundance of some Lactobacillus spp. increased in both the sleeve gastrectomy and distal small intestine bypass groups after surgery. The gut abundance of Lactobacillus was significantly correlated with the improvement in glycolipid metabolism and the change in serum fibroblast growth factor 21 levels.