Published online May 27, 2023. doi: 10.4240/wjgs.v15.i5.917
Peer-review started: October 9, 2022
First decision: December 12, 2022
Revised: December 22, 2022
Accepted: April 4, 2023
Article in press: April 4, 2023
Published online: May 27, 2023
Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) is an innovative surgical approach for the treatment of massive hepatocellular carcinoma (HCC), the key to successful planned stage 2 ALPPS is future liver remnant (FLR) volume growth, but the exact mechanism has not been elucidated. The correlation between regulatory T cells (Tregs) and postoperative FLR regeneration has not been reported.
To investigate the effect of CD4+CD25+ Tregs on FLR regeneration after ALPPS.
Clinical data and specimens were collected from 37 patients who developed massive HCC treated with ALPPS. Flow cytometry was performed to detect changes in the proportion of CD4+CD25+ Tregs to CD4+ T cells in peripheral blood before and after ALPPS. To analyze the relationship between peripheral blood CD4+CD25+ Treg proportion and clinicopathological information and liver volume.
The postoperative CD4+CD25+ Treg proportion in stage 1 ALPPS was negatively correlated with the amount of proliferation volume, proliferation rate, and kinetic growth rate (KGR) of the FLR after stage 1 ALPPS. Patients with low Treg proportion had significantly higher KGR than those with high Treg proportion (P = 0.006); patients with high Treg proportion had more severe postoperative pathological liver fibrosis than those with low Treg proportion (P = 0.043). The area under the receiver operating characteristic curve between the percentage of Tregs and proli
CD4+CD25+ Tregs in the peripheral blood of patients with massive HCC at stage 1 ALPPS were negatively correlated with indicators of FLR regeneration after stage 1 ALPPS and may influence the degree of fibrosis in patients’ livers. Treg percentage was highly accurate in predicting the FLR regeneration after stage 1 ALPPS.
Core Tip: To investigate the mechanisms affecting future liver remnant (FLR) after stage 1 associating liver partition and portal vein ligation for staged hepatectomy (ALPPS), this study was conducted by analyzing clinical data and peripheral blood specimens collected from hepatocellular carcinoma patients treated with ALPPS. The results showed that CD4+CD25+ regulatory T cells (Tregs) in peripheral blood after stage 1 ALPPS was negatively correlated with the index of FLR regeneration after stage 1 ALPPS and may influence the extent of liver fibrosis in patients. The percentage of Tregs was highly accurate in predicting FLR regeneration after stage 1 ALPPS.