Icariin accelerates bone regeneration by inducing osteogenesis-angiogenesis coupling in rats with type 1 diabetes mellitus

doi:10.4239/wjd.v15.i4.769

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World Journal of Diabetes

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1948-9358

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Diabetes. Apr 15, 2024; 15(4): 769-782
Published online Apr 15, 2024. doi: 10.4239/wjd.v15.i4.769

Table 1 Real-time polymerase chain reaction primer sequences

Gene	Forward primer	Reverse primer
ALP	ACCATTCCCACGTCTTCACATTT	AGACATTCTCTCGTTCACCGCC
OCN	GTCAGACTACAACATCCAGAAG	CGAGTATCTTCCTGTTTGACC
RUNX2	GAGCGTTCAACGGCACAG	GACAGTAGACTCCACGACA
COL1A1	TGTCGTTCAACGGCACAG	TGTGGTAGACTCCACGACA
VEGFA	TCAGGAGGACCTTGTGTGATCAG	CATTGCTCTGTACCTTGGGAA
CD31	CACCGTGATACTGAACAGCAA	GTCACAATCCCACCTTCTGTC
Ang-2	GAAGAAGGAGATGGTGGAGA	CGTCTGGTTGAGCAAACTG
Ang-4	GCTCCTCAGGGCACCAAGTTC	CACAGGCGTCAAACCACCAC
GAPDH	GGCATGGACTGTGGTCATGAG	TGCACCACCAACTGTTAGC

ALP: Alkaline phosphatase; Ang-2: Angiopoietin-2; Ang-4: Angiopoietin-4; CD31: Platelet endothelial cell adhesion molecule 1; COL1A1: Collagen type I alpha 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; OCN: Osteocalcin; RUNX2: Runt-related transcription factor 2; VEGFA: Vascular endothelial growth factor A.

Citation: Zheng S, Hu GY, Li JH, Zheng J, Li YK. Icariin accelerates bone regeneration by inducing osteogenesis-angiogenesis coupling in rats with type 1 diabetes mellitus. World J Diabetes 2024; 15(4): 769-782
URL: https://www.wjgnet.com/1948-9358/full/v15/i4/769.htm
DOI: https://dx.doi.org/10.4239/wjd.v15.i4.769