Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Diabetes. Apr 15, 2024; 15(4): 769-782
Published online Apr 15, 2024. doi: 10.4239/wjd.v15.i4.769
Icariin accelerates bone regeneration by inducing osteogenesis-angiogenesis coupling in rats with type 1 diabetes mellitus
Sheng Zheng, Guan-Yu Hu, Jun-Hua Li, Jia Zheng, Yi-Kai Li
Sheng Zheng, Guan-Yu Hu, Yi-Kai Li, Department of Traditional Chinese Orthopedics and Traumatology, Center for Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China
Jun-Hua Li, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Jia Zheng, Department of Endocrinology, Peking University First Hospital, Beijing 100034, China
Co-corresponding authors: Jia Zheng and Yi-Kai Li.
Author contributions: Zheng J and Li YK contributed equally to this work and are co-corresponding authors on this paper according to the critical roles they played throughout the research in experimental design, data analysis, and provision of intellectual ideas and methods; Zheng S, Zheng J, and Li YK designed the research study; Zheng S, Hu GY, and Li JH performed the research; Li JH and Zheng J contributed new reagents and analytic tools; Zheng S, Zheng J, and Li YK analyzed the data and wrote the manuscript; and all authors read and approved the final manuscript.
Supported by the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation, No. GZC20231088; and President Foundation of The Third Affiliated Hospital of Southern Medical University, China, No. YP202210.
Institutional animal care and use committee statement: The study was reviewed and approved by the Animal Ethics Committee of Southern Medical University (Approval No. SMUL2022023).
Conflict-of-interest statement: The authors declare having no competing interests for this article.
Data sharing statement: Data will be made available upon reasonable request to the corresponding author.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Yi-Kai Li, MD, PhD, Chief Physician, Full Professor, Professor, Research Scientist, Department of Traditional Chinese Orthopedics and Traumatology, Center for Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, No. 183 Zhongshan Avenue West, Tianhe District, Guangzhou 510630, Guangdong Province, China. ortho@smu.edu.cn
Received: December 31, 2023
Peer-review started: December 31, 2023
First decision: January 16, 2024
Revised: January 22, 2024
Accepted: March 5, 2024
Article in press: March 5, 2024
Published online: April 15, 2024
Processing time: 102 Days and 17.6 Hours
ARTICLE HIGHLIGHTS
Research background

Icariin (ICA) has multiple pharmacological activities. However, its effect on bone defect repair in the context of type 1 diabetes mellitus (T1DM) remains unclear.

Research motivation

ICA possesses the ability to promote osteogenesis and exert protective effects in T1DM. Therefore, ICA may have therapeutic potential for repairing bone defects in patients with T1DM.

Research objectives

To explore the role of ICA on bone defect repair in T1DM models.

Research methods

The effects of ICA on osteogenesis and angiogenesis were evaluated by molecular biology techniques in vitro. After a T1DM rat model was established, we evaluated the effect of ICA on bone formation in a defect area.

Research results

ICA promoted bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and induced osteogenesis-angiogenesis coupling of BMSCs in vitro. Subsequently, we observed that ICA facilitated bone formation and type H vessel formation in the defect area of the T1DM rat model. Sequential fluorescent labeling confirmed that ICA could effectively accelerate the rate of bone formation in the defect area.

Research conclusions

ICA accelerated bone regeneration by inducing osteogenesis-angiogenesis coupling of BMSCs in the T1DM rat model.

Research perspectives

Our study highlighted the importance of effective coupling of osteogenesis and angiogenesis in bone regeneration in the context of T1DM.