Comprehensive analysis of endoplasmic reticulum stress-related mechanisms in type 2 diabetes mellitus

Figure 1 Study flow chart. GSEA: Gene set enrichment analysis; GSVA: Gene set variation analysis; ER: Endoplasmic reticulum; DEG: Differentially expressed genes; PPI: Protein–protein interaction; TF: Transcription factor.

Figure 2 Gene set enrichment analysis. A: Top five gene set enrichment analysis in proliferating myoblasts; B: Neuroactive ligand–receptor interaction; C: Hypertrophic cardiomyopathy; D: DNA replication; E: Cell cycle; F: Cardiac muscle contraction; G: Bubble plot in proliferating myoblasts; H: Ridgeline plot in proliferating myoblasts; I: Top 5 gene set enrichment analysis in differentiated myotubes; J: Viral myocarditis; K: Steroid hormone biosynthesis; L: Hematopoietic cell lineage; M: Focal adhesion; N: Extracellular matrix–receptor interaction; O: Bubble plot in differentiated myotubes; P: Ridgeline plot in differentiated myotubes.

Figure 3 Gene set variation analysis. A: Volcano plot of proliferating myoblasts; B: Volcano plot of differentiated myotubes; C: Heat map of proliferating myoblasts; D: Heat map of differentiated myotubes.

Figure 4 Endoplasmic reticulum stress-related differentially expressed genes. A: Heat map of proliferating myoblasts; B: Volcano plot of proliferating myoblasts; C: Heat map of differentiated myotubes; D: Volcano plot of differentiated myotubes; E: Endoplasmic reticulum stress-related differentially expressed genes. ER: Endoplasmic reticulum.

Figure 5 Functional enrichment analysis. A: Gene Ontogeny biological processes; B: Gene Ontogeny cellular components; C: Gene Ontogeny molecular function; D: Kyoto Encyclopedia of Genes and Genomes.

Figure 6 Protein–protein interaction network. A: Protein–protein interaction (PPI) network; B: PPI network by NetworkAnalyzer; C: PPI network of 20 key genes.

Figure 7 Networks. A: Transcription factor–mRNA network; B: miRNA–mRNA network; C: Drug–mRNA network.

Figure 8 Immune infiltration. A: Histogram of immune infiltration distribution; B: Histogram of immune infiltration sample distribution; C: Heat map of immune infiltration correlation; D: Correlation diagram of 20 key genes.