Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Diabetes. Apr 15, 2024; 15(4): 735-757
Published online Apr 15, 2024. doi: 10.4239/wjd.v15.i4.735
Novel insights into immune-related genes associated with type 2 diabetes mellitus-related cognitive impairment
Jing Gao, Ying Zou, Xiao-Yu Lv, Li Chen, Xin-Guo Hou
Jing Gao, Ying Zou, Xiao-Yu Lv, Li Chen, Xin-Guo Hou, Department of Endocrinology, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong Province, China
Xin-Guo Hou, Institute of Endocrine and Metabolic Diseases, Shandong University, Jinan 250012, Shandong Province, China
Xin-Guo Hou, Key Laboratory of Endocrine and Metabolic Diseases, Shandong Province Medicine & Health, Jinan 250012, Shandong Province, China
Xin-Guo Hou, Department of Endocrinology, Jinan Clinical Research Center for Endocrine and Metabolic Disease, Jinan 250012, Shandong Province, China
Author contributions: The design of this study was carried out by Hou XG; the collection and analysis of bioinformatics data, experimental validation, and writing of the manuscript were carried out by Gao J; Zou Y took on the task of conducting statistical analysis on the experimental data; Lv XY was responsible for animal modeling; Chen L contributed to the literature research; the final manuscript was read and approved by all the authors.
Supported by National Natural Science Foundation of China, No. 82270845.
Institutional review board statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Qilu Medical College of Shandong University (IACUC protocol number: 23001).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Qilu Medical College of Shandong University (IACUC protocol number: 23001).
Conflict-of-interest statement: The authors assert that there are no conflicting interests.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Xin-Guo Hou, MD, PhD, Chief, Chief Doctor, Department of Endocrinology, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, No. 107 Wenhua Xilu, Jinan 250012, Shandong Province, China. houxinguo@sdu.edu.cn
Received: November 11, 2023
Peer-review started: November 11, 2023
First decision: January 15, 2024
Revised: January 21, 2024
Accepted: March 4, 2024
Article in press: March 4, 2024
Published online: April 15, 2024
Processing time: 152 Days and 8.4 Hours
Core Tip

Core Tip: Using GSE125387, we identified differentially expressed genes in the hippocampus of T2DM mice and controls. Fifty-nine genes were identified through functional enrichment analysis and protein-protein interactions analysis. Machine learning was utilized to identify three hub genes (H2-T24, Rac3, and Tfrc). And the three hub genes were validated in GSE152539. Validation experiments were conducted at the mRNA and protein levels both in vivo and in vitro. Additionally, 11 potential compands associated with RAC3 and TFRC were identified based on the Comparative Toxicogenomics Database. The findings provide new insights into the treatment of T2DM-related cognitive impairment.