Muñoz-Islas E, Santiago-SanMartin ED, Mendoza-Sánchez E, Torres-Rodríguez HF, Ramírez-Quintanilla LY, Peters CM, Jiménez-Andrade JM. Long-term effects of gestational diabetes mellitus on the pancreas of female mouse offspring. World J Diabetes 2024; 15(4): 758-768 [PMID: 38680692 DOI: 10.4239/wjd.v15.i4.758]
Corresponding Author of This Article
Juan Miguel Jiménez-Andrade, PhD, Professor, Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, 16 Calle y Lago de Chapala, Col. Aztlán, Reynosa 88740, Tamaulipas, Mexico. jandrade@docentes.uat.edu.mx
Research Domain of This Article
Research & Experimental Medicine
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Diabetes. Apr 15, 2024; 15(4): 758-768 Published online Apr 15, 2024. doi: 10.4239/wjd.v15.i4.758
Long-term effects of gestational diabetes mellitus on the pancreas of female mouse offspring
Enriqueta Muñoz-Islas, Edgar David Santiago-SanMartin, Eduardo Mendoza-Sánchez, Héctor Fabián Torres-Rodríguez, Laura Yanneth Ramírez-Quintanilla, Christopher Michael Peters, Juan Miguel Jiménez-Andrade
Enriqueta Muñoz-Islas, Edgar David Santiago-SanMartin, Eduardo Mendoza-Sánchez, Héctor Fabián Torres-Rodríguez, Laura Yanneth Ramírez-Quintanilla, Juan Miguel Jiménez-Andrade, Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, Reynosa 88740, Tamaulipas, Mexico
Christopher Michael Peters, Department of Anesthesiology, Wake Forest University School of Medicine, Winston Salem, NC 27101, United States
Author contributions: Muñoz-Islas E, Jiménez-Andrade JM, and Peters CM designed and coordinated the study; Muñoz-Islas E contributed to funding acquisition; Santiago-SanMartin ED, Mendoza-Sánchez E, Torres-Rodríguez HF, and Ramírez-Quintanilla LY performed the experiments and acquired and analyzed data; Muñoz-Islas E, Jiménez-Andrade JM, and Peters CM drafted the manuscript; and all authors contributed to the interpretation of the results and critical review of the paper, and approved the final version of the article.
Supported bythe National Council for Humanities, Science and Technology of Mexico CONAHCyT, No. CB/2017-2018/A1-S-27869.
Institutional animal care and use committee statement: All animal experiments were conducted following the national guidelines and the relevant national laws on the protection of animals and were approved by the Institutional Research and Ethics Committee of Unidad Académica Multidisciplinaria Reynosa-Aztlán (Approval No. CEI-UAMRA-2021-0003).
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: Technical appendix, statistical code, and dataset available by request (jandrade@docentes.uat.edu.mx). Not additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Juan Miguel Jiménez-Andrade, PhD, Professor, Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipas, 16 Calle y Lago de Chapala, Col. Aztlán, Reynosa 88740, Tamaulipas, Mexico. jandrade@docentes.uat.edu.mx
Received: December 12, 2023 Peer-review started: December 12, 2023 First decision: January 15, 2024 Revised: January 29, 2024 Accepted: March 11, 2024 Article in press: March 11, 2024 Published online: April 15, 2024 Processing time: 121 Days and 3.6 Hours
ARTICLE HIGHLIGHTS
Research background
Epidemiological studies have shown several long-term neurological, cardiovascular, and endocrinological complications of gestational diabetes mellitus (GDM) in offspring.
Research motivation
Although there are several reports about the metabolic long-term complications of GDM on offspring, there is scarce information about the pathological changes at a cellular level that occur in the pancreas of offspring born from dams with GDM.
Research objectives
To quantify a subset of sensory and sympathetic nerve fibers, macrophages, and vasculature in the pancreas from adult offspring born from mouse dams with GDM.
Research methods
GDM was induced by i.p. administration of streptozotocin (STZ) in ICR mouse dams. Adult female offspring born from dams with and without GDM were sacrificed and pancreata were processed for immunohistochemistry. There was a quantification of the density of sensory (CGRP) and sympathetic (TH) axons, blood vessels (endomucin), and macro-phages (CD68) in the splenic pancreas using confocal microscopy.
Research results
Offspring mice born from STZ-treated dams had similar body weight and blood glucose values compared to offspring born from vehicle-treated dams. However, the density of CGRP+ and TH+ axons, endomucin+ blood vessels, and CD68+ macrophages in the exocrine pancreas was significantly greater in offspring from mothers with GDM vs control offspring. Likewise, the microvasculature in the islets was significantly greater, but not the number of macrophages within the islets of offspring born from dams with GDM compared to control mice.
Research conclusions
GDM induces neuronal, vascular, and inflammatory changes in the pancreas of adult progeny, which may partially explain the higher propensity for offspring of mothers with GDM to develop metabolic diseases.
Research perspectives
Future studies are needed to evaluate the functional implications of these alterations and determine if their blockade with mechanism-based therapies may decrease the risk of developing impaired glucose tolerance and type-2 diabetes in individuals born from women with GDM.
