Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Diabetes. Dec 15, 2021; 12(12): 2050-2057
Published online Dec 15, 2021. doi: 10.4239/wjd.v12.i12.2050
Inhibitory effect of maspinon neovascularization in diabetic retinopathy
Feng Qiu, Hui-Juan Tong
Feng Qiu, Department of Ophthalmology, Shenyang Fourth People’s Hospital, Shenyang 110031, Liaoning Province, China
Hui-Juan Tong, Department of Nursing, Shenyang Medical College, Shenyang 110034, Liaoning Province, China
Author contributions: Qiu F was involved in the data curation and writing of the original draft; Tong HJ performed the data curation and formal analysis, and participated in the writing and editing of the manuscript; All authors have read and approved the final.
Supported by Liaoning Province Natural Science Foundation, No. 2020-BS-277.
Institutional animal care and use committee statement: The study was reviewed and approved by Shenyang Fourth People’s Hospital Institutional Review Board.
Conflict-of-interest statement: The authors declare that they have no conflicting interests.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Hui-Juan Tong, PhD, Assistant Professor, Department of Nursing, Shenyang Medical College, No. 146 North Huanghe Avenue, Shenyang 110034, Liaoning Province, China. thj_9921@163.com
Received: July 7, 2021
Peer-review started: July 7, 2021
First decision: July 28, 2021
Revised: August 9, 2021
Accepted: December 8, 2021
Article in press: December 8, 2021
Published online: December 15, 2021
Processing time: 161 Days and 21.5 Hours
ARTICLE HIGHLIGHTS
Research background

Diabetic retinopathy (DR) is a serious and potentially blinding complication of diabetes mellitus.

Research motivation

We used an experimental animal model to find a more effective strategy for the treatment of DR.

Research objectives

The study aim was to evaluate the effect of intravitreal injection of recombinant human maspin on neovascularization in DR.

Research methods

An oxygen-induced retinopathy (OIR) model in mice was used to simulate neovascularization in diabetic retinopathy. On postnatal day P12, 0.5 µL of 0.05 mg/mL recombinant human maspin was injected into the vitreous cavity of maspin injection OIR group mice. The protein and mRNA expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1-alpha (HIF-1α) in the retina were assayed. The numbers of vascular cell nuclei that broke through the inner limiting membrane were counted.

Research results

The results revealed that intravitreal injection of maspin inhibited neovascularization and reduced protein and mRNA expression of VEGF, HIF-1α in the retinal tissue of OIR model mice.

Research conclusions

Maspin inhibited neovascularization of DR by modulating the VEGF/HIF-1α pathway, providing a potential and effective strategy for the treatment of DR.

Research perspectives

Retinal neovascularization is one of the main pathological features of PDR. Inhibiting retinal neovascularization is a research focus.