Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Diabetes. Jun 15, 2020; 11(6): 239-251
Published online Jun 15, 2020. doi: 10.4239/wjd.v11.i6.239
CD47 decline in pancreatic islet cells promotes macrophage-mediated phagocytosis in type I diabetes
Jing Zhang, Su-Bee Tan, Zhi-Gang Guo
Jing Zhang, Zhi-Gang Guo, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210097, Jiangsu Province, China
Jing Zhang, Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, United States
Su-Bee Tan, National Key Laboratory for Biochemistry, College of Life Sciences, Nanjing University, Nanjing 210093, Jiangsu Province, China
Author contributions: Guo ZG and Zhang J conceived and designed the study; Zhang J and Tan SB performed the experiments and wrote the manuscript; Guo ZG and Tan SB revised the manuscript.
Supported by the National Natural Science Foundation of China, No. 31701179; the China Postdoctoral Science Foundation, No. 2016M591877.
Institutional animal care and use committee statement: The Institutional Animal Care and Use Committee of Nanjing Normal University provided approval for this study.
Conflict-of-interest statement: The authors declare that they have no conflicts of interest.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Zhi-Gang Guo, PhD, Professor, Senior Research Fellow, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210097, Jiangsu Province, China. guozgang@gmail.com
Received: November 7, 2019
Peer-review started: November 7, 2019
First decision: December 12, 2019
Revised: March 30, 2020
Accepted: April 18, 2020
Article in press: April 18, 2020
Published online: June 15, 2020
ARTICLE HIGHLIGHTS
Research background

Type I diabetes (T1D) is characterized by insulin loss, accompanied by excessive inflammatory cell infiltration like macrophages and the destruction of the pancreas. Regarding the mechanochemical signaling regulation of T1D, the relationship between macrophage migration and phagocytosis is still unclear. In this study, we provided a new insight into the immune response occurring in the pancreas.

Research motivation

We try to provide a new insight into the mechanism of immune response occurring in the pancreas of T1D patients.

Research objectives

Our aim was to provide a new strategy to prevent T1D progression.

Research methods

This study was performed both in vivo and in vitro. Macrophage migration and infiltration were assayed to study the mechanism of T1D immune response. The statistical analysis was performed using SPSS statistical software (version 16.0).

Research results

In this study, we found a significant decrease of CD47 in pancreatic beta islet cells stimulated with STZ and enhanced migration and infiltration of macrophages. As an integrin-associated surface factor, CD47 expression level is strongly related to the microphage immune response to inflamed pancreas beta islet.

Research conclusions

Our study shows a new mechanistic insight into T1D from view of immune response.

Research perspectives

This study could provide a new strategy to prevent the progression of T1D.