Copyright
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
Evaluation of oxidative stress levels in obesity and diabetes by the free oxygen radical test and free oxygen radical defence assays and correlations with anthropometric and laboratory parameters
Mihnea-Alexandru Găman, Mirela Elena Epîngeac, Camelia Cristina Diaconu, Amelia Maria Găman
Mihnea-Alexandru Găman, Camelia Cristina Diaconu, "Carol Davila" University of Medicine and Pharmacy, Bucharest 050474, Romania
Mihnea-Alexandru Găman, Department of Hematology, Center of Hematology and Bone Marrow Transplantation, Fundeni Clinical Institute, Bucharest 022328, Romania
Mirela Elena Epîngeac, Amelia Maria Găman, Department of Pathophysiology, University of Medicine and Pharmacy of Craiova, Craiova 200349, Romania
Camelia Cristina Diaconu, Internal Medicine Clinic, Clinical Emergency Hospital of Bucharest, Bucharest 014461, Romania
Amelia Maria Găman, Clinic of Hematology, Filantropia City Hospital, Craiova 200143, Romania
Author contributions: Găman AM, Găman MA and Epîngeac ME designed the study; Epîngeac ME, Găman MA and Găman AM collected the data; Epîngeac ME and Găman AM performed the measurements and contributed reagents; Găman MA analysed the data; Găman MA and Epîngeac ME wrote the paper; Găman AM and Diaconu CC critically revised the paper for scientific content; All authors read and approved the final version of the manuscript; Găman MA and Epîngeac ME contributed equally to this work.
Institutional review board statement: The Ethics Committee of the University of Medicine and Pharmacy of Craiova, Craiova, Romania approved the current study (approval number: 40/27.03.2018).
Informed consent statement: All the subjects involved in the current study agreed to partake in the research and gave their written informed consent. All procedures and experiments were carried out in accordance with the national law and the Helsinki Declaration of 1975, as revised in 2008(5).
Conflict-of-interest statement: The authors have no conflicts of interest to disclose.
STROBE statement: The authors certify that the manuscript adheres to the STROBE statement.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Mihnea-Alexandru Găman, MD, Doctor, "Carol Davila" University of Medicine and Pharmacy, 8 Eroii Sanitari Boulevard, Bucharest 050474, Romania. mihneagaman@yahoo.com
Received: January 1, 2020
Peer-review started: January 1, 2020
First decision: January 15, 2020
Revised: March 24, 2020
Accepted: March 28, 2020
Article in press: March 28, 2020
Published online: May 15, 2020
ARTICLE HIGHLIGHTS
Research background
Oxidative stress is a key player in health and disease, and its particular involvement in the development of obesity, type 2 diabetes mellitus, cardiovascular disorders, neurodegeneration and cancer have attracted much attention from the scientific community in recent years.
Research motivation
The motivation for our research was to contribute to the study of oxidative stress involvement in obesity, diabetes and their co-occurrence (diabesity), and to improve the current knowledge regarding the development of these public health problems.
Research objectives
The main objectives of this study were to evaluate oxidative stress levels in obesity, diabetes and diabesity using the free oxygen radical test (FORT) and the free oxygen radical defence (FORD) tests. In addition, we investigated whether FORT and (or) FORD values correlated with anthropometric and laboratory parameters.
Research methods
Oxidative stress was evaluated from a single drop of capillary blood using the CR3000 analyser by two colorimetric assays: The free oxygen radical test (FORT) and the free oxygen radical defence (FORD) assays. Demographic, clinical and biochemical parameters were assessed by standard methods.
Research results
FORT levels were higher in obese subjects vs healthy controls and correlated positively with body mass index, waist circumference, fasting plasma glucose, total cholesterol and uric acid. FORD levels were lower in obese subjects vs healthy controls and correlated negatively with body mass index, waist circumference, fasting plasma glucose, total cholesterol and uric acid. Patients with diabesity had higher FORT values vs non-diabetic counterparts. In these subjects, FORT levels correlated positively with body mass index and total cholesterol, and FORD levels was negatively associated with body mass index and total cholesterol.
Research conclusions
Oxidative stress levels are increased in obese subjects. In patients with diabesity, reactive oxygen species are elevated vs obese non-diabetic subjects and controls.
Research perspectives
Further studies are needed to clarify the role of oxidative stress in obesity, diabetes and diabesity, and to transpose these results from bench to bedside. The value of antioxidants in the management of these public health problems needs further clarification.
