Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Diabetes. Oct 15, 2024; 15(10): 2123-2134
Published online Oct 15, 2024. doi: 10.4239/wjd.v15.i10.2123
Effect of cuproptosis on acute kidney injury after cardiopulmonary bypass in diabetic patients
Xi-Jin Deng, Yi-Nan Wang, Chuan-Bao Lv, Zhong-Zhi Qiu, Ling-Xin Zhu, Jing-Hui Shi, Si-Ri-Gu-Leng Sana
Xi-Jin Deng, Department of Anesthesiology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Yi-Nan Wang, Department of The Health Management Service Evaluation Center, The Health Management Service Evaluation Center of Heilongjiang Province, Harbin 150000, Hei-longjiang Province, China
Chuan-Bao Lv, Department of Anesthesiology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 150001, Guangdong Province, China
Zhong-Zhi Qiu, Jing-Hui Shi, Si-Ri-Gu-Leng Sana, Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Ling-Xin Zhu, Department of Thoracic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
Co-first authors: Xi-Jin Deng and Yi-Nan Wang.
Author contributions: Sana SRGL designed the study, collected data, and wrote and revised the manuscript; Wang YN, Deng XJ, Lv CB, Qiu ZZ interpreted and analyzed the data; Zhu LX, Shi JH collected the data.
Supported by the Natural Science Foundation of Heilongjiang Province, No. LH2022H035; and the First Affiliated Hospital of Harbin Medical University Foundation, No. 2024JQ14.
Institutional review board statement: This study was approved by the Institutional Ethics Committee of the Harbin Medical University, this study is registered at Chinese Clinical Trial Registry, No. ChiCTR2000038703.
Institutional animal care and use committee statement: The animal experiments conformed to the guidelines of the National Institutes of Health on the Care and Use of Animals and the Animal Management Committee of the Harbin Medical University, No. 2022IIT037.
Conflict-of-interest statement: The authors declare that they have no conflict of interest.
Data sharing statement: The datasets generated during and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Si-Ri-Gu-Leng Sana, PhD, Professor, Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, No. 23 You Zheng Street, Harbin 150001, Heilongjiang Province, China. sana820816@163.com
Received: July 10, 2024
Revised: August 11, 2024
Accepted: September 5, 2024
Published online: October 15, 2024
Processing time: 77 Days and 21.3 Hours
Abstract
BACKGROUND

Cardiopulmonary bypass (CPB) is a common procedure in cardiac surgery. CPB is a high-risk factor for acute kidney injury (AKI), and diabetes is also such a factor. Diabetes can lead to copper overload. It is currently unclear whether AKI after CPB in diabetic patients is related to copper overload.

AIM

To explore whether the occurrence of CPB-AKI in diabetic patients is associated with cuproptosis.

METHODS

Blood and urine were collected from clinical diabetic and non-diabetic patients before and after CPB. Levels of copper ion, lactate, glucose, heat shock protein-70 (HSP-70), and dihydrolipoamide dehydrogenase (DLAT) were determined. A diabetic rat model was established and CPB was performed. The rats were assessed for the development of CPB-AKI, and for the association of AKI with cuproptosis by detecting copper levels, iron-sulfur cluster proteins and observation of mitochondrial structure by electron microscopy.

RESULTS

CPB resulted in elevations of copper, lactate, HSP-70 and DLAT in blood and urine in both diabetic and non-diabetic patients. CPB was associated with pathologic and mitochondrial damage in the kidneys of diabetic rats. Cuproptosis-related proteins also appeared to be significantly reduced.

CONCLUSION

CPB-AKI is associated with cuproptosis. Diabetes mellitus is an important factor aggravating CPB-AKI and cuproptosis.

Keywords: Cardiopulmonary bypass; Acute kidney injury; Cuproptosis; Diabetes; Copper overload; Iron-sulfur cluster proteins

Core Tip: This study found that cardiopulmonary bypass (CPB) resulted in elevations of copper, lactate, heat shock protein-70 and dihydrolipoamide dehydrogenase in blood and urine in both diabetic and non-diabetic patients. CPB was associated with pathologic and mitochondrial damage in the kidneys of diabetic rats. Cuproptosis-related proteins also appeared to be significantly reduced. CPB- acute kidney injury (AKI) is associated with cuproptosis. Diabetes mellitus is an important factor aggravating CPB-AKI and cuproptosis. We believe this manuscript is valuable for all the researchers who are interested in.