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Fayolle K, Girard C, Lasfargues P, Koteich S, Kerros S. Comparison of In Vitro Methods for Assaying the Antibacterial Activity of a Mix of Natural Essential Oils Against Zoonotic Bacteria. Microorganisms 2025; 13:1125. [PMID: 40431297 PMCID: PMC12114583 DOI: 10.3390/microorganisms13051125] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/06/2025] [Revised: 04/16/2025] [Accepted: 04/29/2025] [Indexed: 05/29/2025] Open
Abstract
With the increasing occurrence of bacterial resistance, it is now essential to look for new alternatives to protect the curative utilization of antibiotics within the One Health concept. Here, we adapt and optimize a broth microdilution method and compare it against the broth macrodilution method for evaluating the antibacterial activity of a complex essential oils mix (EO mix) against four livestock pathogens: Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus. Microdilution method performance (final volume well: 300 µL; inoculum: 1.0 × 106 CFU/mL) was evaluated following CLSI recommendations, by comparing the MIC of each of the four strains with the MICs obtained with the macrodilution method (final volume tube: 2 mL; inoculum 1.0 × 106 CFU/mL). Microdilution analysis was performed with an automated plate reader (Bioscreen C), and three bacterial growth parameters (OD max, lag phase, and growth rate) were calculated (DMFit curve-fitting software (v2.1; courtesy of the Institute of Food Research, Norwich, UK)). EO mix MICs were determined for E. coli, S. aureus, and B. cereus. Our results emphasize the importance of ensuring the accuracy of MIC results by performing three technical and three biological replicates, and combining OD max, lag phase, and growth rate to assess the impact of an EO mix at sub-MIC levels.
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Affiliation(s)
- Karine Fayolle
- INRAE, VetAgro Sup Campus Agronomique de Lempdes, UMR F, Université Clermont Auvergne, 15000 Aurillac, France
| | - Claire Girard
- Microlab—Phytosynthese, 63200 Mozac, France; (C.G.); (P.L.); (S.K.)
| | | | - Sahar Koteich
- Microlab—Phytosynthese, 63200 Mozac, France; (C.G.); (P.L.); (S.K.)
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Dobutr T, Jangpromma N, Patramanon R, Daduang J, Kulchat S, Areemit J, Lomthaisong K, Daduang S. Screening of aqueous plant extracts for immunomodulatory effects on immune cells and cytokine production: In vitro and in vivo analyses. Heliyon 2025; 11:e42692. [PMID: 40034324 PMCID: PMC11872543 DOI: 10.1016/j.heliyon.2025.e42692] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/02/2024] [Revised: 02/09/2025] [Accepted: 02/12/2025] [Indexed: 03/05/2025] Open
Abstract
This study investigates the immunomodulatory effects of various aqueous plant extracts on immune cells and cytokine production. In vitro, several extracts, including holy basil (Ocimum sanctum), patawali (Tinospora crispa), and Indian borage (Plectranthus amboinicus L.), significantly increased CD3+ T-cell populations, while soap pod (Acacia concinna), garlic (Allium sativum L.), and neem (Azadirachta indica) also boosted CD45RA+ B-cells. In vivo, the extracts had subtle effects on spleen morphology and Peyer's Patches, with milk bush (Euphorbia tirucalli L.) and Indian borage enhancing T-cell responses, while soap pod stimulated B-cells. Additionally, we observed that Neem and milk bush significantly suppressed B-cell populations. Furthermore, cytokine analysis showed that garlic and patawali reduced IL-2, while soap pod, holy basil, and patawali increased TNF-alpha levels. Soap pod also elevated IL-10 and IL-17A, indicating both anti-inflammatory and pro-inflammatory signaling, while patawali induced an increase in IL-4. In conclusion, Thai medicinal plants show strong potential as both immunostimulants and immunosuppressants. They can enhance lymphocyte proliferation, particularly in T-cells, and modulate B-cell populations. Their aqueous extracts play a key role in regulating Th1, Th2, and Th17 cytokine production. Thus, these plants could serve as natural agents and alternative medicines for boosting or modulating immune function.
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Affiliation(s)
- Theerawat Dobutr
- Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, 90000, Thailand
- Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
| | - Nisachon Jangpromma
- Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
- Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
| | - Rina Patramanon
- Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
- Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
| | - Jureerut Daduang
- Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
| | - Sirinan Kulchat
- Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
| | - Jringjai Areemit
- Division of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
| | - Komsorn Lomthaisong
- Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
- Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
| | - Sakda Daduang
- Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
- Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
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Brito-Junior L, Brito HC, Simões MM, Santos B, Marques FMC, Medeiros MAA, Alves MS, Farias JHA, Pereira CT, Diniz AF, Oliveira-Filho AA, Vilela VLR. Evaluation of the antibacterial activity of essential oils from oregano (Origanum vulgare) against Escherichia coli strains isolated from meat products. BRAZ J BIOL 2025; 84:e286183. [PMID: 39813488 DOI: 10.1590/1519-6984.286183] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/02/2024] [Accepted: 09/22/2024] [Indexed: 01/18/2025] Open
Abstract
The objective of this study was to analyze the antimicrobial and anti-stick capacity of essential oil extracted from oregano (Origanum vulgare) in relation to various strains of Escherichia coli (Ec 41, Ec 42, Ec 44, Ec 45) isolated from meat products. Techniques such as Determination of Minimum Inhibitory Concentration were used (MIC) and Minimum Bactericidal Concentration (CBM). Furthermore, the method was used disk diffusion method to examine the interaction between O. vulgare essential oil and synthetic antibiotics. Determination of the Inhibitory Concentration was also carried out Minimum Adhesion (CIMA). The results indicated that O. vulgare oil demonstrated antimicrobial activity against the E. coli strains tested, with values of MIC ranging between 256 μg/mL and 512 μg/mL, and MBC values ranging between 256 μg/mL and 1.024 μg/mL. Regarding associations, it was observed that O. vulgare had an antagonistic effect towards certain antibiotics, mainly ampicillin, showing greater interference from the essential oil. Furthermore, the oil was effective in inhibiting the adherence of E. coli bacterial strains, demonstrating a more significant antibiofilm agent than 0.12% chlorhexidine digluconate, a commonly used antibacterial. In short, O. vulgare essential oil exhibited antimicrobial potential against E. coli strains isolated from meat products, suggesting which, upon more detailed investigations, could be used both in isolation or in combination with synthetic antibiotics to combat infections caused by this pathogen.
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Affiliation(s)
- L Brito-Junior
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - H C Brito
- Universidade Federal da Paraíba - UFPB, João Pessoa, PB, Brasil
| | - M M Simões
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - B Santos
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - F M C Marques
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - M A A Medeiros
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - M S Alves
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - J H A Farias
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - C T Pereira
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - A F Diniz
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | | | - V L R Vilela
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
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Seetharaman J, Priya RA, Philip RR, Muthuraj M, Sankari D. Evaluation of Tridax procumbens Secondary Metabolites Anti-Tuberculosis Activity by In Vitro and In Silico Methods. Curr Microbiol 2024; 82:50. [PMID: 39702691 DOI: 10.1007/s00284-024-04033-9] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/23/2024] [Accepted: 12/10/2024] [Indexed: 12/21/2024]
Abstract
Mycobacterium tuberculosis is a human pathogen that causes Tuberculosis (TB) disease. Researchers have reported the activity of traditional medicinal plants against human pathogens. However, antimycobacterial studies of medicinal plants against M. tuberculosis remain limited. Thus, the purpose of this study is to characterize the phytochemical profile, antibacterial and antimycobacterial activity of Tridax procumbens towards H37Rv. The antibacterial activity was elucidated by the inhibitory zone formed around the disc by performing disk diffusion method. Tridax antimycobacterial activity measured by Microplate Alamar Blue Assay (MABA) infers the sample is sensitive to H37Rv at MIC (minimum inhibitory concentration) 600 µg/mL. BACTEC MGIT 960 DST identifies the sample is susceptible to H37Rv and Rifampicin resistant (RR). Antiproliferative, functional group determination and mechanism of action of secondary metabolites were performed by MTT, Fourier transform infrared spectroscopy (FTIR) and Gas Chromatography-mass spectrometry (GC-MS). The phytocompounds antimycobacterial efficacy is further supported by molecular docking data. The binding interactions of ligands with gyrA gene revealed (S,Z)-Heptadeca-1,9-dien-4,6-diyn-3-ol molecule as a prominent phytocompound with a binding affinity of -6.6 kcal/mol.
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Affiliation(s)
- Jayashri Seetharaman
- Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, 603203, India
| | - R Akshaya Priya
- Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, 603203, India
| | - Reya Rene Philip
- Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, 603203, India
| | - M Muthuraj
- Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry, 605006, India
| | - D Sankari
- Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu, 603203, India.
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Mendes RS, Santos ESDR, de Andrade SD, da Silva GA, Galvão JLM, dos Santos JRDA, Lima EDO, da Silva RB, Cristiano R, da Silva FF, Lima-Junior CG. Choline Chloride-Urea Deep Eutectic Solvent/Cu-Mn Iminodiacetate Coordination Polymer as an Efficient Catalytic System for Synthesis of Morita-Baylis-Hillman Adducts with Antimicrobial Activity. ACS OMEGA 2024; 9:45911-45919. [PMID: 39583655 PMCID: PMC11579785 DOI: 10.1021/acsomega.4c05386] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Grants] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 06/09/2024] [Revised: 10/09/2024] [Accepted: 10/31/2024] [Indexed: 11/26/2024]
Abstract
This work shows the synthesis of a series of Morita-Baylis-Hillman adducts from isatin derivatives via an efficient green approach involving the use of a new catalyst system, a mixture of copper-manganese iminodiacetate 1D coordination polymer (Cu/Mn-IDA) and choline chloride-urea deep eutectic solvent (ChCl/urea 1:2). The adducts 2a-2i were obtained in good to excellent yields (59-97%) with shorter reaction times. The results demonstrate for the first time the synergistic catalytic effect of the combination of deep eutectic solvent and coordination polymer on the Morita-Baylis-Hillman reactions. The catalyst recyclability was investigated, and it was found that Cu/Mn-IDA maintains consistent performance for at least four catalytic cycles. The synthesized compounds were evaluated for their in vitro antimicrobial activity against four bacteria and eight fungi species. Among all, 2a, 2b, 2d, 2e, and 2g showed antifungal and antibacterial activities in 70-83% of the strains tested. The adduct 2e exhibited significant antifungal activity with a minimum inhibitory concentration value of 128 μg mL-1 compared to the standard drug fluconazole (minimum inhibitory concentration, 256 μg mL-1).
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Affiliation(s)
- Rhuan
Karlos Santos Mendes
- Department
of Chemistry, Federal University of Paraíba, Campus I, João Pessoa, Paraíba 58051-900, Brazil
| | | | - Sandro Dutra de Andrade
- Department
of Chemistry, Federal University of Paraíba, Campus I, João Pessoa, Paraíba 58051-900, Brazil
| | | | | | | | - Edeltrudes de Oliveira Lima
- Department
of Pharmaceutical Science, Federal University
of Paraíba, Campus
I, João Pessoa, Paraíba 58051-900, Brazil
| | - Rodolfo B. da Silva
- Postgraduate
Program in Materials Science and Engineering − PPCEM, Federal University of Paraíba, Campus I, João Pessoa, Paraíba 58051-900, Brazil
| | - Rodrigo Cristiano
- Department
of Chemistry, Federal University of Paraíba, Campus I, João Pessoa, Paraíba 58051-900, Brazil
| | - Fausthon Fred da Silva
- Department
of Chemistry, Federal University of Paraíba, Campus I, João Pessoa, Paraíba 58051-900, Brazil
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Santos AAD, Oliveira-Filho AADE, Teixeira BA, Galvão JLFM, Medeiros MAADE, Alves MS, Barbosa DHX, Mafra RP, Vasconcelos U, Lima EO. Evaluation of (-)-Fenchone antimicrobial activity against oral Candida albicans and toxicological parameters: an in silico, in vitro and ex vivo study. AN ACAD BRAS CIENC 2024; 96:e20240273. [PMID: 39504094 DOI: 10.1590/0001-3765202420240273] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/20/2024] [Accepted: 06/28/2024] [Indexed: 11/08/2024] Open
Abstract
Candida albicans is the primary species causing oral candidiasis. Its increasing drug resistance drives the search for more effective antifungal agents. Therefore, we assessed toxicological parameters and the antimicrobial activity and mechanisms of action of the monoterpene (-)-fenchone against oral C. albicans. We conducted an in silico study using PASS online and AdmetSAR, followed by evaluation of antifungal activity through Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), association study with miconazole, and assays with sorbitol and ergosterol. Inhibition of biofilm formation and disruption of preformed biofilm were considered. Toxicity was also assessed through hemolysis assay. The in silico study revealed a higher likelihood of the compound being active for antifungal activity, as well as promising pharmacokinetic and toxicity characteristics. Subsequently, (-)-fenchone exhibited predominantly fungicidal activity (MIC90 = 8 μg/mL; MFC = 16 μg/mL), including against miconazole-resistant C. albicans isolates. The substance does not appear to act by damaging the fungal cell wall or plasma membrane, and exhibited synergy with miconazole. There was activity in inhibiting biofilm formation but not in disrupting preformed biofilm. Finally, the product exerted low hemolytic activity at more than MIC×10. Based on these results, (-)-fenchone may represent a promising therapeutic alternative for oral candidiasis.
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Affiliation(s)
- André A Dos Santos
- Universidade Federal da Paraíba (UFPB), Centro de Ciências da Saúde (CCS), Departamento de Ciências Farmacêuticas (IPeFarM), Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos (PgPNSB), Campus I, Cidade Universitária, Castelo Branco, 58051-900 João Pessoa, PB, Brazil
| | - Abrahão A DE Oliveira-Filho
- Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Campus Patos, Av. Universitária, s/n, Santa Cecília, 58708-110 Patos, PB, Brazil
| | - Bráulio A Teixeira
- Universidade Federal da Paraíba (UFPB), Centro de Ciências da Saúde (CCS), Departamento de Ciências Farmacêuticas (IPeFarM), Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos (PgPNSB), Campus I, Cidade Universitária, Castelo Branco, 58051-900 João Pessoa, PB, Brazil
| | - José Lucas F M Galvão
- Universidade Federal da Paraíba (UFPB), Centro de Ciências da Saúde (CCS), Departamento de Ciências Farmacêuticas (IPeFarM), Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos (PgPNSB), Campus I, Cidade Universitária, Castelo Branco, 58051-900 João Pessoa, PB, Brazil
| | - Maria Alice A DE Medeiros
- Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Campus Patos, Av. Universitária, s/n, Santa Cecília, 58708-110 Patos, PB, Brazil
| | - Millena S Alves
- Universidade Federal de Campina Grande (UFCG), Centro de Saúde e Tecnologia Rural (CSTR), Campus Patos, Av. Universitária, s/n, Santa Cecília, 58708-110 Patos, PB, Brazil
| | - David Henrique X Barbosa
- Universidade Federal do Rio Grande do Norte (UFRN), Centro de Ciências da Saúde (CCS), Departamento de Odontologia (DOD), Programa de Pós-Graduação em Ciências Odontológicas (PPgCO), Av. Salgado Filho, 1787, Lagoa Nova, 59056-000 Natal, RN, Brazil
| | - Rodrigo P Mafra
- Universidade Federal do Rio Grande do Norte (UFRN), Centro de Ciências da Saúde (CCS), Departamento de Odontologia (DOD), Programa de Pós-Graduação em Ciências Odontológicas (PPgCO), Av. Salgado Filho, 1787, Lagoa Nova, 59056-000 Natal, RN, Brazil
| | - Ulrich Vasconcelos
- Universidade Federal da Paraíba (UFPB), Centro de Ciências da Saúde (CCS), Departamento de Ciências Farmacêuticas (IPeFarM), Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos (PgPNSB), Campus I, Cidade Universitária, Castelo Branco, 58051-900 João Pessoa, PB, Brazil
| | - Edeltrudes O Lima
- Universidade Federal da Paraíba (UFPB), Centro de Ciências da Saúde (CCS), Departamento de Ciências Farmacêuticas (IPeFarM), Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos (PgPNSB), Campus I, Cidade Universitária, Castelo Branco, 58051-900 João Pessoa, PB, Brazil
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Pandey B, Thapa S, Kaundinnyayana A, Panta S. Hepatoprotective effects of Juglans regia on carbon tetrachloride-induced hepatotoxicity: In silico/in vivo approach. Food Sci Nutr 2024; 12:6482-6497. [PMID: 39554326 PMCID: PMC11561823 DOI: 10.1002/fsn3.4288] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/21/2024] [Revised: 05/31/2024] [Accepted: 06/04/2024] [Indexed: 11/19/2024] Open
Abstract
Juglans regia L. is a well-known therapeutic plant in Nepal, employed in traditional medicine for treating liver ailments. This study aimed to evaluate the in vivo and in silico liver-protective effects of J. regia extract using a carbon tetrachloride (CCl4)-induced hepatic damage rat model. Healthy male rats were randomly divided into six groups: normal control (distilled water 10 mL/kg), toxic control (distilled water 10 mL/kg), standard test (silymarin 100 mg/kg), and three groups receiving oral J. regia extracts (125, 250, and 500 mg/kg/day) for seven days. On the eighth day, carbon tetrachloride (CCl4) was administered intraperitoneally (i.p.) (1.5 mL/kg in 1:1 olive oil ratio for all groups, except the normal control). Rats were sacrificed on the ninth day, and blood was collected retro-orbitally for liver blood injury tests and histopathological studies. Molecular docking was performed against cytochrome P450 2E1 (CYP450 2E1) enzyme for 16 selected phytoconstituents. J. regia, at doses of 125, 250, and 500 mg/kg, significantly reduced liver enzyme levels (alanine aminotransferase, alkaline phosphatase, direct bilirubin, and total bilirubin), while increasing serum albumin. Histological analysis revealed mitigation of carbon tetrachloride (CCl4)-induced liver injury, reducing fatty degeneration and necrosis. Molecular docking supported the findings, with Beta-sitosterol and Betulinic acid exhibiting the best binding affinity of -9.2 and -9.1 kcal/mol, respectively. In conclusion, result suggests that J. regia showed dose-dependent hepatoprotective activity in CCl4-induced hepatotoxicity and it could be utilized as a promising hepatoprotective agent. This study suggests the hepatoprotective potential of J. regia bark extracts, emphasizing the need for further clinical validation.
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Affiliation(s)
- Bipindra Pandey
- Department of PharmacyMadan Bhandari Academy of Health SciencesHetaudaNepal
- School of Health and Allied SciencesPokhara UniversityPokharaNepal
| | - Shankar Thapa
- Department of PharmacyMadan Bhandari Academy of Health SciencesHetaudaNepal
| | | | - Sushil Panta
- School of Health and Allied SciencesPokhara UniversityPokharaNepal
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Ghosh AK, Dewaele P, Hu H, Panda SK, Luyten W. Antimicrobial efficacy of select medicinal plant extracts from Bangladesh against food‐borne bacterial pathogens. J Food Saf 2024; 44. [DOI: 10.1111/jfs.13147] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/22/2024] [Accepted: 05/26/2024] [Indexed: 01/11/2025]
Abstract
AbstractWe assessed the antimicrobial activity of extracts prepared with four solvents (hexane, acetone, ethanol, water) from 45 medicinal plants used in Bangladesh. Food pathogenic bacteria (Gram‐positive: Bacillus cereus, Listeria innocua, Streptococcus faecalis, and Gram‐negative: Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, and Shigella sonnei) were tested using a broth microdilution method. The extraction yield was highest (26%) for the water extract of Carica papaya and lowest (0.4%) for the acetone extract of Cynodon dactylon. In general, acetone extracts exhibited much more antibacterial activity than those obtained with the other three solvents (hexane, ethanol, and water). Gram‐positive bacteria were more sensitive than Gram‐negatives. Streptococcus faecalis was the most susceptible to inhibition by examined extracts, whereas E. coli and P. aeruginosa were the most resistant. Most inhibitory concentration‐50 (IC50) values ranged between 101 and 500 μg/mL (64 extracts, 35.5%), followed by the range of 501–1000 μg/mL (40 extracts, 22.2%). Based on IC50, the most effective plants were three species of Piper (Piper nigrum, Piper betle, and Piper chaba), followed by Nigella sativa, Psidium guajava, Syzygium cumini, C. dactylon, and Phyllanthus emblica. In addition, the toxicity of chosen extracts against normal and malignant cell lines was tested; the most effective extracts were toxic against the human lung carcinoma cell line A549, but less toxic against the human Caucasian foetal lung cell line WI26VA4. These findings suggest that some plant extracts could be employed to treat food‐borne bacterial infections, or as herbal preservatives in the food sector.
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Affiliation(s)
- Alokesh Kumar Ghosh
- Fisheries and Marine Resource Technology Discipline Khulna University Khulna Bangladesh
| | - Pieter Dewaele
- Animal Physiology and Neurobiology Section, Department of Biology, Faculty of Science KU Leuven Leuven Belgium
| | - Haibo Hu
- School of Pharmacy Gannan Medical University Ganzhou China
| | - Sujogya Kumar Panda
- Center of Environment Climate Change and Public Health Utkal University Bhubaneswar India
| | - Walter Luyten
- Animal Physiology and Neurobiology Section, Department of Biology, Faculty of Science KU Leuven Leuven Belgium
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Worku KM, Araya D, Tesfa H, Birru EM, Hailu A, Aemero M. In vitro antileishmanial activities of hydro-methanolic crude extracts and solvent fractions of Clematis simensis fresen leaf, and Euphorbia abyssinica latex. Medicine (Baltimore) 2024; 103:e38039. [PMID: 38701291 PMCID: PMC11062719 DOI: 10.1097/md.0000000000038039] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/27/2023] [Accepted: 04/05/2024] [Indexed: 05/05/2024] Open
Abstract
As a result of increasing drug resistance, crossover resistance development, prolonged therapy, and the absence of different agents with innovative methods for implementation, the efficacy of recent antileishmanial medications is severely declining. So, it is vital to look for other medications from botanical remedies that have antileishmanial activity. The latex of Euphorbia abyssinica (E abyssinica) and the leaves of Clematis simensis fresen (C simensis) were macerated in methanol (80%). In vitro antileishmanial activity of the preparation was tried on promastigotes of Leishmania aethiopica (L aethiopica) and Leishmania donovani (L donovani) using resazurin assay, and fluorescence intensity was measured. One percent of dimethyl sulfoxide (DMSO) and media as negative control and amphotericin B as positive control were used. Additionally, hemolytic & phytochemical tests of the preparation were done. The mean and standard errors of each extract were evaluated and interpreted for statistical significance using one-way analysis of variance. From sigmoidal dose-response curves of % inhibition, half maximal inhibitory concentration (IC50) values were determined by GraphPad Prism and Microsoft Excel; outcomes were presented as mean ± standard error of mean of triplicate trials. P < .05 was statistical significance. The phytochemical screening of C simensis and E abyssinica confirmed the existence of steroids, phenols, tannins, saponins, alkaloids, terpenoids, flavonoids and glycosides. C simensis possesses antileishmanial activity with IC50 outcomes of 46.12 ± 0.03 and 8.18 ± 0.10 µg/mL on the promastigotes of L aethiopica and L donovani, respectively. However, E abyssinica showed stronger activity with IC50 outcomes of 16.07 ± 0.05 µg/mL and 4.82 ± 0.07 µg/mL on L aethiopica and L donovani, respectively. C simensis and E abyssinica have a less hemolytic effect on human red blood cells at low concentrations. The outcomes from this investigation demonstrated that the preparation of C simensis and E abyssinica indicated significant antileishmanial activity. Therefore, further in vivo assessment of antileishmanial, cytotoxicity activity and quantitative identification of secondary metabolites are highly recommended.
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Affiliation(s)
- Kassahun Misgana Worku
- Department of Medical Laboratory Science, College of Medicine and Health Sciences, Arba Minch University, Arba Minch, Ethiopia
| | - Dawit Araya
- Department of Microbiology, Immunology and Parasitology, Faculty of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
| | - Habtie Tesfa
- Department of Medical Parasitology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia
| | - Eshetie Melese Birru
- Department of Pharmacology, School of Pharmacy, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia
| | - Asrat Hailu
- Department of Microbiology, Immunology and Parasitology, Faculty of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
| | - Mulugeta Aemero
- Department of Medical Parasitology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia
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10
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Kim H, Kim MH, Choi UL, Chung MS, Yun CH, Shim Y, Oh J, Lee S, Lee GW. Molecular and Phenotypic Investigation on Antibacterial Activities of Limonene Isomers and Its Oxidation Derivative against Xanthomonas oryzae pv. oryzae. J Microbiol Biotechnol 2024; 34:562-569. [PMID: 38247219 PMCID: PMC11016764 DOI: 10.4014/jmb.2311.11016] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/13/2023] [Revised: 01/02/2024] [Accepted: 01/05/2024] [Indexed: 01/23/2024]
Abstract
Xanthomonas oryzae pv. oryzae (Xoo) causes a devastating bacterial leaf blight in rice. Here, the antimicrobial effects of D-limonene, L-limonene, and its oxidative derivative carveol against Xoo were investigated. We revealed that carveol treatment at ≥ 0.1 mM in liquid culture resulted in significant decrease in Xoo growth rate (> 40%) in a concentration-dependent manner, and over 1 mM, no growth was observed. The treatment with D-limonene and L-limonene also inhibited the Xoo growth but to a lesser extent compared to carveol. These results were further elaborated with the assays of motility, biofilm formation and xanthomonadin production. The carveol treatment over 1 mM caused no motilities, basal level of biofilm formation (< 10%), and significantly reduced xanthomonadin production. The biofilm formation after the treatment with two limonene isomers was decreased in a concentration-dependent manner, but the degree of the effect was not comparable to carveol. In addition, there was negligible effect on the xanthomonadin production mediated by the treatment of two limonene isomers. Field emission-scanning electron microscope (FE-SEM) unveiled that all three compounds used in this study cause severe ultrastructural morphological changes in Xoo cells, showing shrinking, shriveling, and holes on their surface. Moreover, quantitative real-time PCR revealed that carveol and D-limonene treatment significantly down-regulated the expression levels of genes involved in virulence and biofilm formation of Xoo, but not with L-limonene. Together, we suggest that limonenes and carveol will be the candidates of interest in the development of biological pesticides.
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Affiliation(s)
- Hyeonbin Kim
- Green-Bio Division, Jeonju AgroBio-Materials Institute, Jeonju 54810, Republic of Korea
| | - Mi Hee Kim
- Green-Bio Division, Jeonju AgroBio-Materials Institute, Jeonju 54810, Republic of Korea
| | - Ui-Lim Choi
- Green-Bio Division, Jeonju AgroBio-Materials Institute, Jeonju 54810, Republic of Korea
| | - Moon-Soo Chung
- Division of Radiation Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea
| | - Chul-Ho Yun
- School of Biological Sciences and Technology, Chonnam National University, Gwangju 61186, Republic of Korea
| | - Youngkun Shim
- Microzyme Co., Ltd. Research and Development Department, Damyang-gun, Jeollanam-do 57385, Republic of Korea
| | - Jaejun Oh
- Microzyme Co., Ltd. Research and Development Department, Damyang-gun, Jeollanam-do 57385, Republic of Korea
| | - Sungbeom Lee
- Division of Radiation Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea
- Department of Radiation Science and Technology, University of Science and Technology, Daejeon 34113, Republic of Korea
| | - Gun Woong Lee
- Green-Bio Division, Jeonju AgroBio-Materials Institute, Jeonju 54810, Republic of Korea
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11
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Santos B, Farias JHA, Simões MM, Medeiros MAA, Alves MS, Diniz AF, Soares APO, Cavalcante APTM, Silva BJN, Almeida JCS, Lemos JO, Rocha LES, Santos LC, Azevedo MLG, Vieira SWF, Araújo VE, Oliveira Filho AA. Evaluation of the antimicrobial activity of Eucalyptus radiata essential oil against Escherichia coli strains isolated from meat products. BRAZ J BIOL 2024; 84:e281361. [PMID: 38451631 DOI: 10.1590/1519-6984.281361] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/12/2023] [Accepted: 02/14/2024] [Indexed: 03/08/2024] Open
Abstract
The present study sought to evaluate the antimicrobial and anti-adherent potential of Eucalyptus radiata essential oil against food-borne strains of Escherichia coli. The study was performed using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). In addition, the disk diffusion technique was used to evaluate the association of Eucalyptus radiata essential oil with synthetic antimicrobials. The Minimum Inhibitory Adherence Concentration (MIC) was also performed. The results revealed that E. radiata showed antimicrobial activity against the E. coli strains tested, with MIC values ranging from 500 μg/mL to 1000 μg/mL and MBC values ranging from 500 μg/mL to 1,024 μg/mL. As for the associations, it was observed that E. radiata oil exhibited a synergistic effect for some antibiotics, especially Ceftriaxone, with greater interference from the essential oil. Furthermore, it was effective in inhibiting the adherence of bacterial strains of E. coli, showing a more significant antibiofilm effect than the antibacterial agent 0.12% chlorhexidine digluconate. In summary, the essential oil of E. radiata showed antimicrobial potential against strains of E. coli of food origin, and can therefore, through in-depth studies, be used alone or in association with synthetic antimicrobials to combat infections caused by this pathogen.
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Affiliation(s)
- B Santos
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - J H A Farias
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - M M Simões
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - M A A Medeiros
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - M S Alves
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - A F Diniz
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - A P O Soares
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | | | - B J N Silva
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - J C S Almeida
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - J O Lemos
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - L E S Rocha
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - L C Santos
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - M L G Azevedo
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - S W F Vieira
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - V E Araújo
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
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12
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Zhang C, Zaki FR, Won J, Boppart SA. A multimodal nonlinear optical microscopy study of the responses of Pseudomonas aeruginosa to blue light and antibiotic treatment. JOURNAL OF BIOPHOTONICS 2024; 17:e202300384. [PMID: 38010357 PMCID: PMC10961202 DOI: 10.1002/jbio.202300384] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/16/2023] [Revised: 11/10/2023] [Accepted: 11/14/2023] [Indexed: 11/29/2023]
Abstract
Pseudomonas aeruginosa (P. aeruginosa) is a multidrug-resistant human pathogen involved in numerous infections. Understanding the response of P. aeruginosa to various treatments is critical to developing new ways for the antimicrobial susceptibly test and more effective treatment methods. Conventional antimicrobial susceptibility tests lack molecular information at the single bacterium level. In this study, we used label-free multimodal nonlinear optical microscopy to identify an autofluorescence signal from pyoverdine, a siderophore of the bacteria, for quantification of P. aeruginosa responses to antibiotics and blue light treatment. We also discovered that the bleaching of the pyoverdine autofluorescence signals is correlated with the inactivation of P. aeruginosa and is perhaps one of the mechanisms involved in the blue light inactivation of P. aeruginosa.
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Affiliation(s)
- Chi Zhang
- Beckman Institute for Advanced Science and Technology
| | | | - Jungeun Won
- Beckman Institute for Advanced Science and Technology
- Department of Bioengineering
| | - Stephen A. Boppart
- Beckman Institute for Advanced Science and Technology
- Department of Bioengineering
- Department of Electrical and Computer Engineering
- Cancer Center at Illinois
- Carle Illinois College of Medicine
- NIH/NIBIB Center for Label-free Imaging and Multiscale Biophotonics (CLIMB), University of Illinois Urbana-Champaign
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13
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Ferreira EDS, Cordeiro LV, Silva DDF, Diniz Neto H, Sousa APD, Souza HDDS, Athayde-Filho PFD, Guerra FQS, Barbosa-Filho JM, Oliveira Filho AAD, Lima EDO, Castro RDD. Evaluation of antifungal activity, mechanisms of action and toxicological profile of the synthetic amide 2-chloro- N-phenylacetamide. Drug Chem Toxicol 2024; 47:191-202. [PMID: 36803623 DOI: 10.1080/01480545.2022.2158849] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/20/2022] [Revised: 10/29/2022] [Accepted: 12/10/2022] [Indexed: 02/22/2023]
Abstract
Aspergillus niger causes infections such as otitis and pulmonary aspergillosis in immunocompromised individuals. Treatment involves voriconazole or amphotericin B, and due to the increase in fungal resistance, the search for new compounds with antifungal activity has intensified. In the development of new drugs, cytotoxicity and genotoxicity assays are important, as they allow predicting possible damage that a molecule can cause, and in silico studies predict the pharmacokinetic properties. The aim of this study was to verify the antifungal activity and the mechanism of action of the synthetic amide 2-chloro-N-phenylacetamide against Aspergillus niger strains and toxicity. 2-Chloro-N-phenylacetamide showed antifungal activity against different strains of Aspergillus niger with minimum inhibitory concentrations between 32 and 256 μg/mL and minimum fungicides between 64 and 1024 μg/mL. The minimum inhibitory concentration of 2-chloro-N-phenylacetamide also inhibited conidia germination. When associated with amphotericin B or voriconazole, 2-chloro-N-phenylacetamide had antagonistic effects. Interaction with ergosterol in the plasma membrane is the probable mechanism of action.2-Chloro-N-phenylacetamide has favorable physicochemical parameters, good oral bioavailability and absorption in the gastrointestinal tract, crosses the blood-brain barrier and inhibits CYP1A2. At concentrations of 50 to 500 µg/mL, it has little hemolytic effect and a protective effect for type A and O red blood cells, and in the cells of the oral mucosa it promotes little genotoxic change. It is concluded that 2-chloro-N-phenylacetamide has promising antifungal potential, favorable pharmacokinetic profile for oral administration and low cytotoxic and genotoxic potential, being a promising candidate for in vivo toxicity studies.
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Affiliation(s)
- Elba Dos Santos Ferreira
- Postgraduate Program in Natural and Bioactive Synthetic Products, Health Sciences Center, Federal University of Paraíba, Center for Health Sciences, Federal University of Paraiba, Campus I, University City Allotment, João Pessoa, Brazil
| | - Laísa Vilar Cordeiro
- Postgraduate Program in Natural and Bioactive Synthetic Products, Health Sciences Center, Federal University of Paraíba, Center for Health Sciences, Federal University of Paraiba, Campus I, University City Allotment, João Pessoa, Brazil
| | - Daniele de Figuerêdo Silva
- Postgraduate Program in Natural and Bioactive Synthetic Products, Health Sciences Center, Federal University of Paraíba, Center for Health Sciences, Federal University of Paraiba, Campus I, University City Allotment, João Pessoa, Brazil
| | - Hermes Diniz Neto
- Postgraduate Program in Natural and Bioactive Synthetic Products, Health Sciences Center, Federal University of Paraíba, Center for Health Sciences, Federal University of Paraiba, Campus I, University City Allotment, João Pessoa, Brazil
| | - Aleson Pereira de Sousa
- Postgraduate Program in Technological Development and Innovation of Medicines, Health Sciences Center, Federal University of Paraíba, University City Allotment, João Pessoa, Brazil
| | - Helivaldo Diógenes da Silva Souza
- Department of Chemistry, Center for Exact and Natural Sciences, Federal University of Paraíba, Campus I, University City Allotment, João Pessoa, Brazil
| | - Petrônio Filgueiras de Athayde-Filho
- Department of Chemistry, Center for Exact and Natural Sciences, Federal University of Paraíba, Campus I, University City Allotment, João Pessoa, Brazil
| | | | - José Maria Barbosa-Filho
- Postgraduate Program in Natural and Bioactive Synthetic Products, Health Sciences Center, Federal University of Paraíba, Center for Health Sciences, Federal University of Paraiba, Campus I, University City Allotment, João Pessoa, Brazil
| | - Abrahão Alves de Oliveira Filho
- Biological Sciences Academic Unit at the Center for Rural Health and Technology (CSTR), Federal University of Campina Grande (UFCG), Bairro Jatobá, Patos, Brazil
| | - Edeltrudes de Oliveira Lima
- Postgraduate Program in Natural and Bioactive Synthetic Products, Health Sciences Center, Federal University of Paraíba, Center for Health Sciences, Federal University of Paraiba, Campus I, University City Allotment, João Pessoa, Brazil
| | - Ricardo Dias de Castro
- Postgraduate Program in Natural and Bioactive Synthetic Products, Health Sciences Center, Federal University of Paraíba, Center for Health Sciences, Federal University of Paraiba, Campus I, University City Allotment, João Pessoa, Brazil
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14
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Nazeam JA, El-Emam SZ. Middle Eastern Plants with Potent Cytotoxic Effect Against Lung Cancer Cells. J Med Food 2024; 27:198-207. [PMID: 38381516 DOI: 10.1089/jmf.2022.0098] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 02/22/2024] Open
Abstract
Cancer is one of the leading causes of increasing global mortality with uprising health concerns and threats. Unfortunately, conventional chemotherapy has substantial side effects, limiting its relevance and prompting a quest for safe and efficient alternatives. For thousands of years, plants have provided a rich reservoir for curing a variety of ailments, including cancer. According to the World Health Organization, medicinal plants would be the best source of medications. However, only 25% of drugs in the present pharmacopoeia are derived from plants. Hence, further research into different plants is required to better understand their efficacy. Twenty extracts of widely distributed Middle Eastern plants were screened for the cytotoxic effect against lung cancer cell lines (A549). Eleven plants showed IC50 below 25 μg/mL, consequently, the bioactive extracts were further fractionated by graded precipitation using absolute ethanol. All fraction A (FA; crude polysaccharides precipitate) showed potent IC50, 0.2-5.5 μg/mL except the FA of Brassica juncea, Silybum marianum, and Phaseolus vulgaris, whereas FB fractions (filtrate) of Anastatica hierochuntica, Plantago ovate, Tussilago farfara, and Cucurbita moschata had lower efficacy than other fractions with IC50 values in the range of 0.1-7.7 μg/mL. The fractions of FA Taraxacum officinale and FB Ziziphus spina possess the most potent cytotoxic activity with IC50, 0.2 and 0.1 μg/mL, respectively. Moreover, cell cycle analysis of both fractions revealed an arrest at G1/S-phase and activation of apoptosis rather than necrosis as the mode of cell death. Therefore, T. officinale and Z. spina fractions may pave the way to manage lung carcinoma as an alternative and complementary food regimen.
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Affiliation(s)
- Jilan A Nazeam
- Department of Pharmacognosy, Faculty of Pharmacy, October 6 University, Giza, Egypt
| | - Soad Z El-Emam
- Department of Pharmacology and Toxicology, Faculty of Pharmacy, October 6 University, Giza, Egypt
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15
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Ezzaky Y, Elmoslih A, Silva BN, Bonilla-Luque OM, Possas A, Valero A, Cadavez V, Gonzales-Barron U, Achemchem F. In vitro antimicrobial activity of extracts and essential oils of Cinnamomum, Salvia, and Mentha spp. against foodborne pathogens: A meta-analysis study. Compr Rev Food Sci Food Saf 2023; 22:4516-4536. [PMID: 37615998 DOI: 10.1111/1541-4337.13232] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/12/2023] [Revised: 07/10/2023] [Accepted: 08/07/2023] [Indexed: 08/25/2023]
Abstract
Essential oils (EOs) are a class of natural products that exhibit potent antimicrobial properties against a broad spectrum of bacteria. Inhibition diameters (IDs) and minimum inhibitory concentrations (MICs) are the typical measures of antimicrobial activity for extracts and EOs obtained from Cinnamomum, Salvia, and Mentha species. This study used a meta-analytical regression analysis to investigate the correlation between ID and MIC measurements and the variability in antimicrobial susceptibility tests. By utilizing pooled ID models, this study revealed significant differences in foodborne pathogens' susceptibility to extracts, which were dependent on both the plant species and the methodology employed (p < .05). Cassia showed the highest efficacy against Salmonella spp., exhibiting a pooled ID of 26.24 mm, while cinnamon demonstrated the highest efficacy against Bacillus cereus, with a pooled ID of 23.35 mm. Mint extract showed the greatest efficacy against Escherichia coli and Staphylococcus aureus. Interestingly, cinnamon extract demonstrated the lowest effect against Shiga toxin-producing E. coli, with a pooled ID of only 8.07 mm, whereas its EOs were the most effective against this bacterial strain. The study found that plant species influenced the MIC, while the methodology did not affect MIC measurements (p > .05). An inverse correlation between ID and MIC measurements was identified (p < .0001). These findings suggest that extracts and EOs obtained from Cinnamomum, Salvia, and Mentha spp. have the potential to inhibit bacterial growth. The study highlights the importance of considering various factors that may influence ID and MIC measurements when assessing the effectiveness of antimicrobial agents.
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Affiliation(s)
- Youssef Ezzaky
- Bioprocess and Environment Team, LASIME Laboratory, Agadir Superior School of Technology, Ibn Zohr University, Agadir, Morocco
| | - Abdelkhaleq Elmoslih
- Bioprocess and Environment Team, LASIME Laboratory, Agadir Superior School of Technology, Ibn Zohr University, Agadir, Morocco
| | - Beatriz Nunes Silva
- Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança, Portugal
- Laboratório para a Sustentabilidade e Tecnologia em Regiões de Montanha, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança, Portugal
- CEB - Centre of Biological Engineering, University of Minho, Campus Gualtar, Braga, Portugal
| | - Olga María Bonilla-Luque
- Department of Food Science and Technology, UIC Zoonosis y Enfermedades Emergentes (ENZOEM), CeiA3, Universidad de Córdoba, Campus Rabanales, Córdoba, Spain
| | - Arícia Possas
- Department of Food Science and Technology, UIC Zoonosis y Enfermedades Emergentes (ENZOEM), CeiA3, Universidad de Córdoba, Campus Rabanales, Córdoba, Spain
| | - Antonio Valero
- Department of Food Science and Technology, UIC Zoonosis y Enfermedades Emergentes (ENZOEM), CeiA3, Universidad de Córdoba, Campus Rabanales, Córdoba, Spain
| | - Vasco Cadavez
- Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança, Portugal
- Laboratório para a Sustentabilidade e Tecnologia em Regiões de Montanha, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança, Portugal
| | - Ursula Gonzales-Barron
- Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança, Portugal
- Laboratório para a Sustentabilidade e Tecnologia em Regiões de Montanha, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Bragança, Portugal
| | - Fouad Achemchem
- Bioprocess and Environment Team, LASIME Laboratory, Agadir Superior School of Technology, Ibn Zohr University, Agadir, Morocco
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16
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Osei-Mensah B, Boakye YD, Anyan WK, Agana TA, Aboagye EA, Bentil I, Lomotey ES, Adu F, Agyare C. In Vitro Cercaricidal Activity, Acute Toxicity, and GC/MS Analysis of Some Selected Ghanaian Medicinal Plants. J Parasitol Res 2023; 2023:4589424. [PMID: 37745984 PMCID: PMC10516696 DOI: 10.1155/2023/4589424] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/07/2022] [Revised: 07/28/2023] [Accepted: 08/22/2023] [Indexed: 09/26/2023] Open
Abstract
Schistosomiasis is a human parasitic disease caused by the Schistosoma species and is recognised in public health as second to malaria in terms of its socioeconomic impact on humans. Four local plants native to many tribes in Ghana and known for their medicinal properties against some diseases were assessed for their cercaricidal activity against Schistosoma mansoni cercariae. The plants, namely, Newbouldia laevis stem bark (NLSB), Spathodea campanulata stem bark (SCSB), Momordica charantia leaves (MCL), and Ocimum viride leaves (OVL), were extracted for their active metabolites using methanol. Preliminary phytochemical screening was carried out on all plant extracts and powdered samples. The crude extracts were tested against S. mansoni cercariae in vitro using Balanites aegyptiaca as the positive control. The percentage of mortalities for each extract was recorded. Gas chromatography/mass spectrometry (GC/MS) analysis was conducted on all the plant extracts. Phytochemical analysis revealed the presence of saponins, glycosides, triterpenoids, sterols, alkaloids, flavonoids, and tannins in almost all the extracts. GC/MS analysis showed the presence of medicinally important active volatile compounds in each extract such as thymol, n-hexadecanoic acid, phytol, and maltol. All four plants showed relatively different levels of activity against S. mansoni cercariae at different times and concentrations. The LC50 values of the plant extracts were determined at the end of the assay. At 240 min, NLSB, SCSB, MCL, and OVL extracts had LC50 values of 487.564, 429.898, 197.696, and 0.129 μg/mL, respectively. Hence, this study revealed the potency of Ocimum viride leaves, Momordica charantia leaves, Spathodea campanulata stem bark, and Newbouldia laevis stem bark against S. mansoni. These plants could therefore be exploited as possible candidates for curbing schistosomiasis.
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Affiliation(s)
- Bright Osei-Mensah
- Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
| | - Yaw Duah Boakye
- Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
| | - William Kofi Anyan
- Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
| | - Theresa Appiah Agana
- Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
| | | | - Ivy Bentil
- Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
| | - Elvis Suatey Lomotey
- Department of Parasitology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
| | - Francis Adu
- Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
| | - Christian Agyare
- Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
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17
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Adebayo AA, Ademosun AO, Oboh G. Chemical composition, antioxidant, and enzyme inhibitory properties of Rauwolfia vomitoria extract. JOURNAL OF COMPLEMENTARY & INTEGRATIVE MEDICINE 2023; 20:597-603. [PMID: 37216495 DOI: 10.1515/jcim-2022-0390] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/14/2022] [Accepted: 05/02/2023] [Indexed: 05/24/2023]
Abstract
OBJECTIVES Rauwolfia vomitoria is one ethno-botanicals in Nigeria used by traditional health practitioners in managing several human diseases. However, necessary information regarding its effect on enzymes implicated in the development and progression of erectile dysfunction is missing in the literature. Thus, this study investigated the antioxidant property and impact of Rauwolfia vomitoria extract on erectile dysfunction-related enzymes in vitro. METHODS High performance liquid chromatography was used to identify and quantify Rauwolfia vomitoria's phenolic components. Then, utilizing common antioxidant assays, the extract's antioxidant properties were evaluated and finally the effect of the extract on some enzymes (AChE, arginase and ACE) implicated in erectile dysfunction was investigated in vitro. RESULTS The results showed that the extract inhibited AChE (IC50=388.72 μg/mL), arginase (IC50=40.06 μg/mL) and ACE (IC50=108.64 μg/mL) activities. In addition, phenolic rich extract of Rauvolfia vomitoria scavenged radicals and chelated Fe2+ in concentration dependent manner. Furthermore, rutin, chlorogenic acid, gallic acid, and kaempferol were found in large quantities by HPLC analysis. CONCLUSIONS Therefore, one of the potential reasons driving Rauwolfia vomitoria's use in folk medicine for the treatment of erectile dysfunction could be its antioxidant and inhibitory activities on several enzymes linked to erectile dysfunction in vitro.
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Affiliation(s)
- Adeniyi A Adebayo
- Chemical Science Department (Biochemistry Unit), Joseph Ayo Babalola University, Ikeji-Arakeji, Nigeria
- Functional Foods and Nutraceutical Unit, Biochemistry Department, Federal University of Technology, Akure, Nigeria
| | - Ayokunle O Ademosun
- Functional Foods and Nutraceutical Unit, Biochemistry Department, Federal University of Technology, Akure, Nigeria
| | - Ganiyu Oboh
- Functional Foods and Nutraceutical Unit, Biochemistry Department, Federal University of Technology, Akure, Nigeria
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18
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Acharya S, Jali P, Pradhan M, Pradhan C, Mohapatra PK. Antimicrobial and Antioxidant Property of a True Mangrove Rhizophora apiculata Bl. Chem Biodivers 2023; 20:e202201144. [PMID: 37471640 DOI: 10.1002/cbdv.202201144] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/02/2022] [Revised: 07/19/2023] [Accepted: 07/20/2023] [Indexed: 07/22/2023]
Abstract
Mangroves are abundant in bioactive natural substances that fight off pathogenic diseases. Different parts of R. apiculata, an abundant mangrove found in Bhitarkanika National Park, India were extracted with methanol and a mixture of solvents methanol/ethanol/chloroform (60 : 20 : 20) to evaluate their antimicrobial properties. The combination solvent extract of bark had the highest zone of inhibition (ZOI) of 18.62 mm against Pseudomonas aeruginosa and a ZOI of 17.41 mm against Streptococcus mitis. Bark extracts had the highest DPPH (43 %) and FRAP (96 %) activities. The combination solvent bark extract of R. apiculata had the highest ZOI of 20.42 mm (lowest MIC of 2.12 μg/ml) against Candida albicans and ZOI of 15.33 mm (MIC of 3.02 μg/mL) against Penicillium chrysogenum. Combination bark extracts of R. apiculata contained flavanols than methanolic extracts. The crude extract of R. apiculata bark made with a mixture of solvents containing more active ingredients could be used in novel drug formulation.
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Affiliation(s)
- Srinivas Acharya
- Department of Botany, Utkal University, Bhubaneswar, 751004, Odisha, India
- Department of Environmental Science, Government Autonomous College, Phulbani, Kandhamal, 762001, Odisha, India
| | - Pallavi Jali
- Department of Botany, Utkal University, Bhubaneswar, 751004, Odisha, India
| | - Madhusmita Pradhan
- Majhighariani Institute of Technology and Science, Rayagada, 765017, Odisha, India
| | - Chinmay Pradhan
- Department of Botany, Utkal University, Bhubaneswar, 751004, Odisha, India
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Diniz AF, Santos B, Nóbrega LMMO, Santos VRL, Mariz WS, Cruz PSC, Nóbrega RO, Silva RL, Paula AFR, Santos JRDA, Pessôa HLF, Oliveira-Filho AA. Antibacterial activity of Thymus vulgaris (thyme) essential oil against strains of Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus saprophyticus isolated from meat product. BRAZ J BIOL 2023; 83:e275306. [PMID: 37585936 DOI: 10.1590/1519-6984.275306] [Citation(s) in RCA: 6] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/22/2023] [Accepted: 06/01/2023] [Indexed: 08/18/2023] Open
Abstract
Meat products represent an important component of the human diet and are a good source of nutrients. Food-borne microorganisms are the main pathogens that cause human diseases as a result of food consumption, especially products of animal origin. The objective of the present research was to verify the antibacterial activity of the essential oil of Thymus vulgaris against strains of Klebsiella pneumoniae, Pseudomonas aeruginosa and Staphylococcus saprophyticus isolated from meat products. For this, the analyses of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were performed in microdilution plates. The association of the product with antimicrobials was also studied using disk diffusion. And the anti-adherent activity, which was determined in the presence of sucrose, in glass tubes. Thyme oil showed a strong inhibitory activity against K. pneumoniae, P. aeruginosa and S. saprophyticus, with the MIC values ranging from 64 to 512 μg/mL, and bactericidal effect for most strains, with MBC values ranging from 256 to 1,024 μg/mL. T. vulgaris oil exhibited varied interactions in association with the antimicrobials, with synergistic (41.67%), indifferent (50%) and antagonistic (8.33%) effects. Regarding the anti-adherent activity, the test product was effective in inhibiting the adherence of all bacterial strains under study. Therefore, thyme oil presents itself as an antibacterial and anti-adherent agent against K. pneumoniae, P. aeruginosa and S. saprophyticus, being a natural product that can represent an interesting alternative in the efforts to combat foodborne diseases.
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Affiliation(s)
- A F Diniz
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - B Santos
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - L M M O Nóbrega
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - V R L Santos
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - W S Mariz
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - P S C Cruz
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - R O Nóbrega
- Universidade Federal da Paraíba - UFPB, Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica de Medicamentos, João Pessoa, PB, Brasil
| | - R L Silva
- Universidade Federal da Paraíba - UFPB, Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica de Medicamentos, João Pessoa, PB, Brasil
| | - A F R Paula
- Universidade Federal da Paraíba - UFPB, Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica de Medicamentos, João Pessoa, PB, Brasil
| | - J R D A Santos
- Universidade Federal da Paraíba - UFPB, Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica de Medicamentos, João Pessoa, PB, Brasil
| | - H L F Pessôa
- Universidade Federal da Paraíba - UFPB, Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica de Medicamentos, João Pessoa, PB, Brasil
| | - A A Oliveira-Filho
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
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Manyawi M, Mozirandi WY, Tagwireyi D, Mukanganyama S. Fractionation and Antibacterial Evaluation of the Surface Compounds from the Leaves of Combretum zeyheri on Selected Pathogenic Bacteria. ScientificWorldJournal 2023; 2023:2322068. [PMID: 37520845 PMCID: PMC10382245 DOI: 10.1155/2023/2322068] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/11/2022] [Revised: 03/28/2023] [Accepted: 05/27/2023] [Indexed: 08/01/2023] Open
Abstract
Combretum zeyheri is traditionally used for the treatment of many infections, including bacterial infections. The aim of this study was to fractionate and evaluate antibacterial activity of the crude extract of C. zeyheri, as well as the surface compounds from the leaves of C. zeyheri, in two pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial activities of fractions obtained from chromatographic separations were determined using broth microdilution assay on the laboratory and clinical strains of S. aureus and P. aeruginosa. The fractionation of the compounds on the leaf surface yielded 262 fractions. The fractionated compounds with similar TLC profiles were pooled together to yield 47 pools. The extract and pooled fractions CZSC151154, CZSC155160, and CZSC209213 showed significant antibacterial activity with MIC values ranging from 12.5 μg/ml to 100 μg/ml. The clinical strain of S. aureus had MIC greater than 100 μg/ml for CZSC151154 and CZSC155160. The minimum bactericidal concentration values for these fractions were also in the range of 12.5 μg/ml to 100 μg/ml. The extract and fractions CZSC151154, CZSC155160, and CZSC209213 showed a concentration-dependent inhibition of growth in S. aureus. Analyses of the CZSC209213 pool by LC-MS showed the presence of nine compounds which are (3R,7R)-1,3,7-octanetriol, (-)-tortuosamine, 11-aminoundecanoic acid, 1-piperidinecarboxaldehyde, 3-hydroxy-4-isopropylbenzyl alcohol 3-glucoside, hydroxy-isocaproic acid, oleamide, palmitic amide, phytospingosine, and sphinganine. In conclusion, C. zeyheri leaf surface compounds exhibited antibacterial activity. The crude extract and the pooled fractions showed concentration-dependent inhibition of growth on S. aureus. Results from this study indicate the potential of C. zeyheri as a source of lead compounds that may be further developed into antibacterial drugs.
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Affiliation(s)
- Monica Manyawi
- Department of Pharmacy and Pharmaceutical Sciences, Faculty of Medicine and Health Sciences, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe
| | - Winnie Yevai Mozirandi
- Department of Biotechnology and Biochemistry, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe
| | - Dexter Tagwireyi
- Department of Pharmacy and Pharmaceutical Sciences, Faculty of Medicine and Health Sciences, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe
| | - Stanley Mukanganyama
- Department of Biotechnology and Biochemistry, University of Zimbabwe, Mt. Pleasant, Harare, Zimbabwe
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Sharma A, Nagraik R, Venkidasamy B, Khan A, Dulta K, Kumar Chauhan P, Kumar D, Shin DS. In vitro antidiabetic, antioxidant, antimicrobial, and cytotoxic activity of Murraya koenigii leaf extract intercedes ZnO nanoparticles. LUMINESCENCE 2023; 38:1139-1148. [PMID: 35362206 DOI: 10.1002/bio.4244] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/29/2022] [Revised: 03/24/2022] [Accepted: 03/28/2022] [Indexed: 11/07/2022]
Abstract
Nanotechnology is an emerging field with tremendous potential and usage of medicinal plants and green preparation of nanoparticles (NPs) is one of the widely explored areas. These have been shown to be effective against different biological activities such as diabetes mellitus, cancer, antioxidant, antimicrobial, etc. The current studies focus on the green synthesis of zinc NPs (ZnO NPs) from aqueous leaf extract of Murraya koenigii (MK). The synthesized Murraya koeingii zinc oxide NPs (MK ZnO NPs) were characterized using UV-visible spectroscopy, dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), energy-dispersive spectrum (EDS) and cyclic voltammetry (CV). The synthesized MK ZnO NPs were evaluated for their in vitro antidiabetic, antioxidant, antimicrobial, and cytotoxic activity. They demonstrated significant antidiabetic and cytotoxic activity, as well as moderate free-radical scavenging and antibacterial activity.
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Affiliation(s)
- Avinash Sharma
- Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, India
| | - Rupak Nagraik
- Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, India
| | - Baskar Venkidasamy
- Department of Biotechnology, Sri Shakthi Institute of Engineering and Technology, Coimbatore, Tamil Nadu, India
| | - Azhar Khan
- Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, India
| | - Kanika Dulta
- Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, India
| | - Pankaj Kumar Chauhan
- Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, India
| | - Deepak Kumar
- Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Shoolini University, Solan, India
| | - Dong-Soo Shin
- Department of Chemistry, Changwon National University, Changwon, South Korea
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Mazumder MAR, Tolaema A, Chaikhemarat P, Rawdkuen S. Antioxidant and Anti-Cytotoxicity Effect of Phenolic Extracts from Psidium guajava Linn. Leaves by Novel Assisted Extraction Techniques. Foods 2023; 12:2336. [PMID: 37372547 DOI: 10.3390/foods12122336] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 05/04/2023] [Revised: 06/02/2023] [Accepted: 06/08/2023] [Indexed: 06/29/2023] Open
Abstract
Phytochemicals (PCs) are gaining popularity due to their antioxidant effects and potential protection against infection, cardiovascular disease, and cellular metabolic activity. These PCs must be retained as much as possible during extraction. This research focused on the extraction of PC from Psidium guajava Linn. leaves due to higher antioxidant potential. Solvent extraction (SE), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE) using distilled water (DW) or 60% (v/v) ethanol/water (ET) were used for the extraction of PC. ET shows higher total phenolic (TPC) and total flavonoid content (TFC) as well as higher antioxidant activity than DW. Phytochemical screening demonstrated that all of the screening showed positive results in all extraction methods, except glycoside. There were no significant differences (p > 0.05) in TPC and TFC during MAE/ET, SE/ET, and UAE/ET. Antioxidant analysis shows that MAE and SE resulted in high (p < 0.05) DPPH and FRAP values for ET and DW, respectively. MAE/ET showed the highest inhibitory activity (IC50 = 16.67 µg/mL). HPLC and TLC analysis reveal the fingerprint of morin, which might function as an anticancer agent with other bioactives. Increasing the extract content increased the inhibitory activity of SW480 cells via MTT assay. In conclusion, MAE/ET is the most efficient among the extraction techniques in terms of anti-cytotoxicity effects.
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Affiliation(s)
- Md Anisur Rahman Mazumder
- Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand
- Department of Food Technology and Rural Industries, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
| | - Arif Tolaema
- Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand
| | - Pongpasin Chaikhemarat
- Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand
| | - Saroat Rawdkuen
- Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand
- Unit of Innovative Food Packaging and Biomaterials, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand
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Kalasuba K, Miranti M, Rahayuningsih SR, Safriansyah W, Syamsuri RRP, Farabi K, Oktavia D, Alhasnawi AN, Doni F. Red Mangrove ( Rhizophora stylosa Griff.)-A Review of Its Botany, Phytochemistry, Pharmacological Activities, and Prospects. PLANTS (BASEL, SWITZERLAND) 2023; 12:2196. [PMID: 37299175 PMCID: PMC10255896 DOI: 10.3390/plants12112196] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Received: 05/10/2023] [Revised: 05/30/2023] [Accepted: 05/31/2023] [Indexed: 06/12/2023]
Abstract
Mangroves are ecologically significant plants in marine habitats that inhabit the coastlines of many countries. Being a highly productive and diverse ecosystem, mangroves are rich in numerous classes of phytochemicals that are of great importance in the field of pharmaceutical industries. The red mangrove (Rhizophora stylosa Griff.) is a common member of the Rhizophoraceae family and the dominant species in the mangrove ecosystem of Indonesia. R. stylosa mangrove species are rich in alkaloids, flavonoids, phenolic acids, tannins, terpenoids, saponins, and steroids, and are widely used in traditional medicine for anti-inflammatory, antibacterial, antioxidant, and antipyretic effects. This review aims to provide a comprehensive understanding of the botanical description, phytochemical profiles, pharmacological activities, and medicinal potentials of R. stylosa.
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Affiliation(s)
- Karina Kalasuba
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (K.K.); (S.R.R.); (R.R.P.S.)
| | - Mia Miranti
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (K.K.); (S.R.R.); (R.R.P.S.)
| | - Sri Rejeki Rahayuningsih
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (K.K.); (S.R.R.); (R.R.P.S.)
| | - Wahyu Safriansyah
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (W.S.); (K.F.)
| | - Rizky Riscahya Pratama Syamsuri
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (K.K.); (S.R.R.); (R.R.P.S.)
| | - Kindi Farabi
- Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (W.S.); (K.F.)
| | - Dina Oktavia
- Department of Transdisciplinary, Graduate School, Universitas Padjadjaran, Bandung 40132, Indonesia;
| | - Arshad Naji Alhasnawi
- Department of Biology, College of Education for Pure Sciences, Al-Muthanna University, Samawah 66001, Iraq;
| | - Febri Doni
- Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia; (K.K.); (S.R.R.); (R.R.P.S.)
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24
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Gouveia RG, Oliveira NR, Andrade-Júnior FP, Ferreira RC, Amorim GMW, Silva DKF, Duarte SS, Medeiros CIS, Oliveira-Filho AA, Lima EO. Antifungal effect of (R) and (S)-citronellal enantiomers and their predictive mechanism of action on Candida albicans from voriconazole-resistant onychomycoses. BRAZ J BIOL 2023; 83:e271530. [PMID: 37222371 DOI: 10.1590/1519-6984.271530] [Citation(s) in RCA: 2] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/27/2023] [Accepted: 04/14/2023] [Indexed: 05/25/2023] Open
Abstract
Onychomycosis is the most common disease affecting the nail unit and accounts for at least 50% of all nail diseases. In addition, Candida albicans is responsible for approximately 70% of onychomycoses caused by yeasts. This study investigated the antifungal effect of (R) and (S)-citronellal enantiomers, as well as its predictive mechanism of action on C. albicans from voriconazole-resistant onychomycoses. For this purpose, in vitro broth microdilution and molecular docking techniques were applied in a predictive and complementary manner to the mechanisms of action. The main results of this study indicate that C. albicans was resistant to voriconazole and sensitive to the enantiomers (R) and (S)-citronellal at a dose of 256 and 32 µg/mL respectively. In addition, there was an increase in the minimum inhibitory concentration (MIC) of the enantiomers in the presence of sorbitol and ergosterol, indicating that these molecules possibly affect the integrity of the cell wall and cell membrane of C. albicans. Molecular docking with key biosynthesis proteins and maintenance of the fungal cell wall and plasma membrane demonstrated the possibility of (R) and (S)-citronellal interacting with two important enzymes: 1,3-β-glucan synthase and lanosterol 14α-demethylase. Therefore, the findings of this study indicate that the (R) and (S)-citronellal enantiomers are fungicidal on C. albicans from onychomycoses and probably these substances cause damage to the cell wall and cell membrane of these micro-organisms possibly by interacting with enzymes in the biosynthesis of these fungal structures.
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Affiliation(s)
- R G Gouveia
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
| | - N R Oliveira
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
| | - F P Andrade-Júnior
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
| | - R C Ferreira
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
| | - G M W Amorim
- Universidade Federal da Paraíba - UFPB, Departamento de Ciências Farmacêuticas - DCF, João Pessoa, Paraíba, Brasil
| | - D K F Silva
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
| | - S S Duarte
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
| | - C I S Medeiros
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
| | - A A Oliveira-Filho
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural - CTSR, Patos, Paraíba, Brasil
| | - E O Lima
- Universidade Federal da Paraíba - UFPB, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, João Pessoa, Paraíba, Brasil
- Universidade Federal da Paraíba - UFPB, Departamento de Ciências Farmacêuticas - DCF, João Pessoa, Paraíba, Brasil
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Kato LS, Lelis CA, da Silva BD, Galvan D, Conte-Junior CA. Micro- and nanoencapsulation of natural phytochemicals: Challenges and recent perspectives for the food and nutraceuticals industry applications. ADVANCES IN FOOD AND NUTRITION RESEARCH 2023; 104:77-137. [PMID: 37236735 DOI: 10.1016/bs.afnr.2022.10.003] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/28/2023]
Abstract
Worldwide, there has been growing interest in the research, development, and commercialization of functional bioactive components and nutraceuticals. As a result of consumer awareness of the relationship between diet, health, and disease, the consumption of plant-derived bioactive components has recently increased in the past two decades. Phytochemicals are bioactive nutrient plant chemicals in fruits, vegetables, grains, and other plant foods that may provide desirable health benefits beyond essential nutrition. They may reduce the risk of major chronic diseases, cardiovascular diseases, cancer, osteoporosis, diabetes, high blood pressure, and psychotic diseases and have antioxidant, antimicrobial, and antifungal properties, cholesterol-lowering, antithrombotic, or anti-inflammatory effects. Phytochemicals have been recently studied and explored for various purposes, such as pharmaceuticals, agrochemicals, flavors, fragrances, coloring agents, biopesticides, and food additives. These compounds are known as secondary metabolites and are commonly classified as polyphenols, terpenoids (terpenes), tocotrienols and tocopherols, carotenoids, alkaloids and other nitrogen-containing metabolites, stilbenes and lignans, phenolic acids, and glucosinates. Thus, this chapter aims to define the general chemistry, classification, and essential sources of phytochemicals, as well as describe the potential application of phytochemicals in the food and nutraceuticals industry, explaining the main properties of interest of the different compounds. Finally, the leading technologies involving micro and nanoencapsulation of phytochemicals are extensively detailed to protect them against degradation and enhance their solubility, bioavailability, and better applicability in the pharmaceutical, food, and nutraceutical industry. The main challenges and perspectives are detailed.
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Affiliation(s)
- Lilian Seiko Kato
- Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ, Brazil; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil
| | - Carini Aparecida Lelis
- Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ, Brazil; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Chemistry (PGQu), IQ, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Sanitary Surveillance (PPGVS), National Institute of Health Quality Control (INCQS), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil
| | - Bruno Dutra da Silva
- Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ, Brazil; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil
| | - Diego Galvan
- Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ, Brazil; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Chemistry (PGQu), IQ, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil
| | - Carlos Adam Conte-Junior
- Center for Food Analysis (NAL), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ, Brazil; Laboratory of Advanced Analysis in Biochemistry and Molecular Biology (LAABBM), Department of Biochemistry, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Food Science (PPGCAL), Institute of Chemistry (IQ), UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Chemistry (PGQu), IQ, UFRJ, Cidade Universitária, Rio de Janeiro, RJ, Brazil; Graduate Program in Sanitary Surveillance (PPGVS), National Institute of Health Quality Control (INCQS), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, RJ, Brazil; Graduate Program in Veterinary Hygiene (PPGHV), Faculty of Veterinary Medicine, Fluminense Federal University (UFF), Vital Brazil Filho, Niterói, RJ, Brazil; Residue Analysis Laboratory (LAB RES), Technological Development Support Laboratory (LADETEC), Federal University of Rio de Janeiro (UFRJ), Cidade Universitária, Rio de Janeiro, RJ, Brazil.
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26
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Goel H, Kumar R, Tanwar P, Upadhyay TK, Khan F, Pandey P, Kang S, Moon M, Choi J, Choi M, Park MN, Kim B, Saeed M. Unraveling the therapeutic potential of natural products in the prevention and treatment of leukemia. Biomed Pharmacother 2023; 160:114351. [PMID: 36736284 DOI: 10.1016/j.biopha.2023.114351] [Citation(s) in RCA: 10] [Impact Index Per Article: 5.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/09/2023] [Revised: 01/30/2023] [Accepted: 01/31/2023] [Indexed: 02/04/2023] Open
Abstract
Leukemia is a heterogeneous group of hematological malignancies distinguished by differentiation blockage and uncontrolled proliferation of myeloid or lymphoid progenitor cells in the bone marrow (BM) and peripheral blood (PB). There are various types of leukemia in which intensive chemotherapy regimens or hematopoietic stem cell transplantation (HSCT) are now the most common treatments associated with severe side effects and multi-drug resistance in leukemia cells. Therefore, it is crucial to develop novel therapeutic approaches with adequate therapeutic efficacy and selectively eliminate leukemic cells to improve the consequences of leukemia. Medicinal plants have been utilized for ages to treat multiple disorders due to their diverse bioactive compounds. Plant-derived products have been used as therapeutic medication to prevent and treat many types of cancer. Over the last two decades, 50 % of all anticancer drugs approved worldwide are from natural products and their derivatives. Therefore this study aims to review natural products such as polyphenols, alkaloids, terpenoids, nitrogen-containing, and organosulfur compounds as antileukemic agents. Current investigations have identified natural products efficiently destroy leukemia cells through diverse mechanisms of action by inhibiting proliferation, reactive oxygen species production, inducing cell cycle arrest, and apoptosis in both in vitro, in vivo, and clinical studies. Current investigations have identified natural products as suitable promising chemotherapeutic and chemopreventive agents. It played an essential role in drug development and emerged as a possible source of biologically active metabolites for therapeutic interventions, especially in leukemia. DATA AVAILABILITY: Data will be made available on request.
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Affiliation(s)
- Harsh Goel
- Department of Laboratory Oncology, All India Institute of Medical Sciences, New Delhi 11023, India.
| | - Rahul Kumar
- Department of Laboratory Oncology, All India Institute of Medical Sciences, New Delhi 11023, India.
| | - Pranay Tanwar
- Department of Laboratory Oncology, All India Institute of Medical Sciences, New Delhi 11023, India.
| | - Tarun Kumar Upadhyay
- Department of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, India,.
| | - Fahad Khan
- Department of Biotechnology, Noida Institute of Engineering & Technology, Greater Noida 201306, India.
| | - Pratibha Pandey
- Department of Biotechnology, Noida Institute of Engineering & Technology, Greater Noida 201306, India.
| | - Sojin Kang
- Department of Pathology, College of Korean Medicine, Kyung Hee University, 05253, Republic of Korea.
| | - Myunghan Moon
- Department of Pathology, College of Korean Medicine, Kyung Hee University, 05253, Republic of Korea.
| | - Jinwon Choi
- Department of Pathology, College of Korean Medicine, Kyung Hee University, 05253, Republic of Korea.
| | - Min Choi
- Department of Pathology, College of Korean Medicine, Kyung Hee University, 05253, Republic of Korea.
| | - Moon Nyeo Park
- Department of Pathology, College of Korean Medicine, Kyung Hee University, 05253, Republic of Korea.
| | - Bonglee Kim
- Department of Pathology, College of Korean Medicine, Kyung Hee University, 05253, Republic of Korea.
| | - Mohd Saeed
- Department of Biology, College of Sciences, University of Hail, P.O. Box 2440, Hail 81411 Saudi Arabia.
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Moomin A, Russell WR, Knott RM, Scobbie L, Mensah KB, Adu-Gyamfi PKT, Duthie SJ. Season, storage and extraction method impact on the phytochemical profile of Terminalia ivorensis. BMC PLANT BIOLOGY 2023; 23:162. [PMID: 36964494 PMCID: PMC10039578 DOI: 10.1186/s12870-023-04144-8] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Figures] [Subscribe] [Scholar Register] [Received: 12/27/2022] [Accepted: 02/28/2023] [Indexed: 06/18/2023]
Abstract
BACKGROUND Terminalia ivorensis (TI) is used in West African ethnomedicine for the treatment of conditions including ulcers, malaria and wounds. Despite its widespread use, the phytochemical profile of TI remains largely undetermined. This research investigated the effects of extraction method, season, and storage conditions on the phytochemical composition of TI to contribute towards understanding the potential benefits. METHODS TI bark was collected in September 2014, September 2018 and February 2018 during the rainy or dry seasons in Eastern Region, Ghana. Samples were extracted sequentially with organic solvents (petroleum ether, chloroform, ethyl acetate and ethanol) or using water (traditional). Metabolites were identified by liquid chromatography-mass spectrometry/mass spectrometry and compared statistically by ANOVA. RESULTS A total of 82 different phytochemicals were identified across all samples. A greater yield of the major phytochemicals (44%, p < 0.05) was obtained by water as compared with organic extraction. There was also a higher concentration of metabolites present in cold (63%, p < 0.05) compared with hot water extraction. A significantly (p < 0.05) higher number of phytochemicals were identified from TI collected in the dry (85%) compared to the rainy season (69%). TI bark stored for four years retained 84% of the major phytochemicals. CONCLUSION This work provides important information on composition and how this is modified by growing conditions, storage and method of extraction informing progress on the development of TI as a prophylactic formulation or medicine.
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Affiliation(s)
- Aliu Moomin
- School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, AB10 7GJ UK
- University of Aberdeen, Rowett Institute, Foresterhill Campus, Ashgrove Road West, Scotland, AB25 2ZD UK
| | - Wendy R. Russell
- University of Aberdeen, Rowett Institute, Foresterhill Campus, Ashgrove Road West, Scotland, AB25 2ZD UK
| | - Rachel M. Knott
- School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, AB10 7GJ UK
| | - Lorraine Scobbie
- University of Aberdeen, Rowett Institute, Foresterhill Campus, Ashgrove Road West, Scotland, AB25 2ZD UK
| | - Kwesi Boadu Mensah
- Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
| | - Paa Kofi Tawiah Adu-Gyamfi
- Faculty of Health and Allied Sciences, Department of Nursing and Midwifery, Pentecost University College, Accra, Ghana
| | - Susan J. Duthie
- School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen, AB10 7GJ UK
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Soussi M, El Yaagoubi W, Nekhla H, El Hanafi L, Squalli W, Benjelloun M, El Ghadraoui L. A Multidimensional Review of Pimpinella anisum and Recommendation for Future Research to Face Adverse Climatic Conditions. CHEMISTRY AFRICA 2023. [PMCID: PMC10035495 DOI: 10.1007/s42250-023-00633-3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 03/25/2023]
Abstract
Anise, or Pimpinella anisum, has a long history of usage in traditional and alternative medicine, as well as in the pharmaceutical sector. The species is native to Southwest Asia, the Middle East, and the Mediterranean region. The current studies have revealed that wild and cultivated P. anisum are rich in bioactive compounds, such as phenols, tannins, carotenoids, and fatty acids. Different parts of P. anisum plant are used treatment of diseases, the treatment of pathogens for antiviral, antibacterial, and antifungal purposes, and cosmetics. In this paper, we reviewed the current knowledge on geographical distribution, cultivation, chemical compounds, extraction methods, and agronomic and medicinal uses of Pimpinella anisum. Equally, we suggested guidelines for future research to fill the gap in less investigated issues. Data for this paper was gathered via electronic literature search on Google, Google Scholar, and databases counting, Science Direct, Scopus, Springer, PubMed, and Hindawi, from 1930 to 2022. Analyzed data show that Pimpinella anisum is currently recorded from Portugal on the Atlantic Ocean in the west to China in the East. With new extraction methods, such as Ultrasound-Assisted Extraction, scientists have explored the higher quantities of polyphenols, flavonoids, and antioxidants, and revealed new constituents such as trans-anethole and estragole found in essential oil fruits by gas chromatography-mass spectrometry (GC–MS). These compounds showed significant therapeutic benefits against diseases, pathogens, and cosmetic roles. However, with the current impacts of climate change on natural vegetation cover, more investigations are needed to understand how these affect the geographical distribution, metabolic aspects, and chemical properties of Pimpinella anisum. These are suggested to increase the value of the species and ensure its conservation.
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Affiliation(s)
- Meriem Soussi
- Laboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, University of Sidi Mohamed Ben Abdellah, Fez, Morocco
| | - Wissal El Yaagoubi
- Laboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, University of Sidi Mohamed Ben Abdellah, Fez, Morocco
| | - Houria Nekhla
- Laboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, University of Sidi Mohamed Ben Abdellah, Fez, Morocco
| | - Laila El Hanafi
- Laboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, University of Sidi Mohamed Ben Abdellah, Fez, Morocco
| | - Wafae Squalli
- Laboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, University of Sidi Mohamed Ben Abdellah, Fez, Morocco
| | - Meryem Benjelloun
- Laboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, University of Sidi Mohamed Ben Abdellah, Fez, Morocco
| | - Lahsen El Ghadraoui
- Laboratory of Functional Ecology and Environmental Engineering, Faculty of Sciences and Technology, University of Sidi Mohamed Ben Abdellah, Fez, Morocco
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Antimicrobial Potential of Betulinic Acid and Investigation of the Mechanism of Action against Nuclear and Metabolic Enzymes with Molecular Modeling. Pathogens 2023; 12:pathogens12030449. [PMID: 36986372 PMCID: PMC10058483 DOI: 10.3390/pathogens12030449] [Citation(s) in RCA: 9] [Impact Index Per Article: 4.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/24/2022] [Revised: 02/13/2023] [Accepted: 03/10/2023] [Indexed: 03/18/2023] Open
Abstract
Natural products have important pharmacological activities. This study sought to investigate the activity of the compound betulinic acid (BA) against different strains of bacteria and fungi. The minimum inhibitory concentration (MIC) was determined and then the minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC). After performing the in vitro tests, molecular modeling studies were carried out to investigate the mechanism of action of BA against the selected microorganisms. The results showed that BA inhibited the growth of microbial species. Among the 12 species (Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa, Escherichia coli, Mycobacterium tuberculosis, Candida albicans, C. tropicalis, C. glabrata, Aspergillus flavus, Penicillium citrinum, Trichophyton rubrum, and Microsporum canis) investigated, 9 (75%) inhibited growth at a concentration of 561 µM and 1 at a concentration of 100 µM. In general, the MBC and MFC of the products were between 561 and 1122 μM. In silico studies showed that BA presented a mechanism of action against DNA gyrase and beta-lactamase targets for most of the bacteria investigated, while for fungi the mechanism of action was against sterol 14α-demethylase (CYP51) targets and dihydrofolate reductase (DHFR). We suggest that BA has antimicrobial activity against several species.
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de-Andrade SD, Andrade IMG, Castro A, Montenegro YMR, das-Neves-Moreira D, Maia RA, Martins FT, Vaz BG, Franco-dos-Santos G, Lima EDO, Oliveira NDR, Farias BKDS, Lima-Junior CG. Deep eutectic solvent co-catalyzed synthesis and antimicrobial activity of Morita-Baylis-Hillman adducts from isatin derivatives. J Mol Struct 2023. [DOI: 10.1016/j.molstruc.2022.134323] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/25/2022]
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Silva SL, Araújo FSM, Silva POA, Silva EVA, Bezerra MMSL, Diniz AF, Oliveira DM, Jesus HO, Nascimento Junior BB, Medeiros LADM, Oliveira Filho AA. Evaluation of the antimicrobial effect of the Origanum vulgare L essential oil on strains of Klebsiella pneumoniae. BRAZ J BIOL 2023; 83:e269317. [PMID: 36722663 DOI: 10.1590/1519-6984.269317] [Citation(s) in RCA: 3] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/07/2022] [Accepted: 12/30/2022] [Indexed: 01/28/2023] Open
Abstract
Bacteria may be the initial cause of certain pathologies as well as a secondary agent responsible for the development of complications such as pressure ulcer infections. Pressure ulcers are a persistent health problem, especially in immunocompromised patients, and associated with infection by opportunistic microorganisms with antimicrobial resistance, such as Klebsiella pneumoniae, highlight the need for the development of new antimicrobial approaches. Thus, the aim of this study was to evaluate the antibacterial and anti-adherent activity of Origanum vulgare L. (oregano) essential oil against Klebsiella pneumoniae strains, as well as the effect of its association with synthetic antimicrobials. To this end, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) analyses were performed on microdilution plates. The assay of the Minimum Inhibitory Adherence Concentration (MIAC), with test tubes. As well as, the association study through the infusion disc method containing ampicillin (AMP), gentamicin (GEN), ciprofloxacin (CIP) and ceftriaxone (CEF). Therefore, it was possible to obtain that the essential oil of oregano presents antimicrobial and bactericidal activity, with MIC ranging between 128μg/mL and 256 μg/mL and MBC between 256 μg/mL and 512 μg/mL, on the tested K. pneumoniae strains. When used in association with ampicillin and gentamicin, oregano essential oil showed synergistic effect for some strains. Therefore, it is observed that the tested essential oil can act as a promising antibacterial in the treatment of diseases caused by K. pneumoniae.
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Affiliation(s)
- S L Silva
- Universidade Federal da Paraíba - UFPB, Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos - PPgDITM, João Pessoa, PB, Brasil
| | - F S M Araújo
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Departamento de Odontologia, Patos, PB, Brasil
| | - P O A Silva
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Departamento de Odontologia, Patos, PB, Brasil
| | - E V A Silva
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Departamento de Odontologia, Patos, PB, Brasil
| | - M M S L Bezerra
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Departamento de Odontologia, Patos, PB, Brasil
| | - A F Diniz
- Universidade Federal de Campina Grande-UFCG, Centro de Saúde e Tecnologia Rural, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - D M Oliveira
- Universidade Estadual do Sudoeste da Bahia - UESB, Programa de Pós-graduação em Química - PGQUIM, Jequié, BA, Brasil
| | - H O Jesus
- Universidade Estadual do Sudoeste da Bahia - UESB, Programa de Pós-graduação em Química - PGQUIM, Jequié, BA, Brasil
| | - B B Nascimento Junior
- Universidade Estadual do Sudoeste da Bahia - UESB, Programa de Pós-graduação em Química - PGQUIM, Jequié, BA, Brasil
| | - L A D M Medeiros
- Universidade Federal de Campina Grande - UFCG, Centro de Saúde e Tecnologia Rural, Departamento de Odontologia, Patos, PB, Brasil
| | - A A Oliveira Filho
- Universidade Federal da Paraíba - UFPB, Programa de Pós-graduação em Desenvolvimento e Inovação Tecnológica em Medicamentos - PPgDITM, João Pessoa, PB, Brasil
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Biodiversity, Biochemical Profiling, and Pharmaco-Commercial Applications of Withania somnifera: A Review. Molecules 2023; 28:molecules28031208. [PMID: 36770874 PMCID: PMC9921868 DOI: 10.3390/molecules28031208] [Citation(s) in RCA: 18] [Impact Index Per Article: 9.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/21/2022] [Revised: 01/16/2023] [Accepted: 01/19/2023] [Indexed: 01/28/2023] Open
Abstract
Withania somnifera L. Dunal (Ashwagandha), a key medicinal plant native to India, is used globally to manage various ailments. This review focuses on the traditional uses, botany, phytochemistry, and pharmacological advances of its plant-derived constituents. It has been reported that at least 62 crucial and 48 inferior primary and secondary metabolites are present in the W. somnifera leaves, and 29 among these found in its roots and leaves are chiefly steroidal compounds, steroidal lactones, alkaloids, amino acids, etc. In addition, the whole shrub parts possess various medicinal activities such as anti-leukotriene, antineoplastic, analgesic, anti-oxidant, immunostimulatory, and rejuvenating properties, mainly observed by in vitro demonstration. However, the course of its medical use remains unknown. This review provides a comprehensive understanding of W. somnifera, which will be useful for mechanism studies and potential medical applications of W. somnifera, as well as for the development of a rational quality control system for W. somnifera as a therapeutic material in the future.
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Molina-Cortés A, Quimbaya M, Toro-Gomez A, Tobar-Tosse F. Bioactive compounds as an alternative for the sugarcane industry: Towards an integrative approach. Heliyon 2023; 9:e13276. [PMID: 36816322 PMCID: PMC9932480 DOI: 10.1016/j.heliyon.2023.e13276] [Citation(s) in RCA: 4] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/15/2022] [Revised: 12/15/2022] [Accepted: 01/24/2023] [Indexed: 01/27/2023] Open
Abstract
Here, a comprehensive review of sugarcane industrialization and its relationship with bioactive compounds (BCs) detected in various products and by-products generated during its processing is presented. Furthermore, it is discussed how these compounds have revealed important antioxidant, antineoplastic, antidiabetic, and antimicrobial activities. From this bibliographic research highlights the significance of two types of BCs of natural origin (phenolic compounds (PCs) and terpenoids) and a group of compounds synthesized during industrial transformation processes (Maillard reaction products (MRPs)). It was found that most of the studies about the BCs from sugarcane have been conducted by identifying, isolating, and analyzing ones or a few compounds at a specific period, this being a conventional approach. However, given the complexity of the synthesis processes of all these BCs and the biological activities they can manifest in a specific biological context, novel approaches are needed to address these analyses holistically. To overcome this challenge, integrating massive and multiscale methods, such as omics sciences, seems necessary to enrich these studies. This work is intended to contribute to the state of the art that could support future research about the exploration, characterization, or evaluation of different bioactive molecules from sugarcane and its derivatives.
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Affiliation(s)
- Andrea Molina-Cortés
- Facultad de Ingeniería y Ciencias - Doctorado en Ingeniería y Ciencias Aplicadas, Pontificia Universidad Javeriana Cali, Cali, Colombia
| | - Mauricio Quimbaya
- Facultad de Ingeniería y Ciencias - Doctorado en Ingeniería y Ciencias Aplicadas, Pontificia Universidad Javeriana Cali, Cali, Colombia,Facultad de Ingeniería y Ciencias - Departamento de Ciencias Naturales y Matemáticas, Pontificia Universidad Javeriana Cali, Cali, Colombia
| | - Angie Toro-Gomez
- Facultad de Ciencias Naturales, Exactas y de la Educación - Maestría en Bioingeniería, Universidad del Cauca, Popayán, Colombia
| | - Fabian Tobar-Tosse
- Facultad de Ingeniería y Ciencias - Doctorado en Ingeniería y Ciencias Aplicadas, Pontificia Universidad Javeriana Cali, Cali, Colombia,Facultad de Ciencias de la Salud - Departamento de Ciencias Básicas de la Salud, Pontificia Universidad Javeriana Cali, Cali, Colombia,Corresponding author. Facultad de Ciencias de la Salud - Departamento de Ciencias Básicas de la Salud, Pontificia Universidad Javeriana Cali, Cali, Colombia.
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Phytochemical Composition and Antioxidant Activity of Edible Wild Fruits from Malawi. BIOMED RESEARCH INTERNATIONAL 2023. [DOI: 10.1155/2023/2621434] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 01/27/2023]
Abstract
The purpose of this study was to investigate the phytochemical composition and antioxidant activity of 17 edible wild fruits that are widely distributed and consumed in Malawi for pharmacological value exploration. Qualitative phytochemical analysis, total phenolic content, total flavonoid content, ferric reducing antioxidant power (FRAP), total antioxidant activity (TAA), and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (DPPH) were performed in aqueous and methanolic fruit extracts. The results showed that the extracts contained alkaloids, saponins, terpenoids, glycosides, coumarins, phenolic compounds, tannins, flavonoids, steroids, and quinones. Piliostigma thonningii had the highest total phenolic content (
mg GAEg-1 FW) in methanolic extracts, and Annona senegalensis gave the highest levels of total flavonoid content (
mg RE g-1 FW) in aqueous extracts. The results of antioxidant activities (FRAP, TAA, and DPPH) varied widely, and the variations were significant (
). Thespesia garckeana and Mangifera indica exhibited a high ability to chelate metal cations in methanolic extracts and in aqueous extracts, respectively. DPPH levels were higher in aqueous extracts and ranged from 11.07% to 99.61%. This study provides evidence that the studied edible fruits of Malawi have potential as value-added products for various treatments of oxidative stress-associated ailments as they contain more phytochemical constituents. We recommend further studies to determine if the presence of a particular class of phytochemicals would translate into the bioactivity capability of these edible fruits.
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Medeiros MAA, Alves MS, Santos B, Silva EVA, Araújo FSM, Bezerra MMSL, Silva POA, Rêgo VGS, Pessôa HLF, Oliveira Filho AA. Evaluation of the antibacterial activity of trans-anethole against Enterococcus cloacae and Enterococcus faecalis strains of food origin. BRAZ J BIOL 2023; 83:e269245. [PMID: 36629625 DOI: 10.1590/1519-6984.269245] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/03/2022] [Accepted: 12/01/2022] [Indexed: 01/11/2023] Open
Abstract
The present study sought to evaluate the antibacterial activity of trans-anethole against food-borne strains of Enterobacter cloacae and Enterococcus faecalis. The study was performed using Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC) methods, in addition, disc diffusion technique was used to evaluate the association of trans-anethole with synthetic antimicrobials. Minimum Inhibitory Concentration for Adherence (MICA) testing was also performed. The results revealed that trans-anethole presents no antibacterial activity at any of the concentrations used against the E. cloacae strains tested. However, trans-anethole presented antibacterial effect against five of the six E. faecalis bacterial strains tested, with MIC values ranging from 500 μg/mL to 1000 μg/mL. Further, when analyzing the MBC results against E. faecalis, it was observed that the compound presented values ranging from 500 μg/mL to 1000 μg/mL. As for the associations, it was observed that trans-anethole when combined with the antimicrobials ampicillin, gentamicin, ciprofloxacin, and ceftriaxone presented synergistic effect against most strains of E. faecalis. However, both trans-anethole and the control chlorhexidine (0.12%) presented no antibiofilm effects against strains of E. faecalis. In short, trans-anethole presented potential antibacterial against E. faecalis strains of food origin, and may upon further study, it may be used alone or in association with synthetic antimicrobials to combat infections caused by this bacterium.
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Affiliation(s)
- M A A Medeiros
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - M S Alves
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - B Santos
- Universidade Federal de Campina Grande - UFCG, Programa de Pós-graduação em Ciência e Saúde Animal, Patos, PB, Brasil
| | - E V A Silva
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - F S M Araújo
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - M M S L Bezerra
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - P O A Silva
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - V G S Rêgo
- Universidade Federal de Campina Grande - UFCG, Patos, PB, Brasil
| | - H L F Pessôa
- Universidade Federal da Paraíba - UFPB, João Pessoa, PB, Brasil
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Bhatti SA, Hussain MH, Mohsin MZ, Mohsin A, Zaman WQ, Guo M, Iqbal MW, Siddiqui SA, Ibrahim SA, Ur-Rehman S, Korma SA. Evaluation of the antimicrobial effects of Capsicum, Nigella sativa, Musa paradisiaca L., and Citrus limetta: A review. FRONTIERS IN SUSTAINABLE FOOD SYSTEMS 2022. [DOI: 10.3389/fsufs.2022.1043823] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/23/2022] Open
Abstract
The extensive use of antibiotics and vaccines against microbial infections can result in long-term negative effects on humans and the environment. However, there are a number of plants that have antimicrobial effects against various disease-causing microbes such as bacteria, viruses, and fungi without negative side effects or harm to the environment. In this regard, four particular plants- Capsicum, Nigella sativa, Musa paradisiaca L., and Citrus limetta have been widely considered due to their excellent antimicrobial effect and ample availability. In this review, we discuss their antimicrobial effects due to the presence of thymoquinone, p-cymene, pinene, alkaloids, limonene, camphene, and melanin. These antimicrobial compounds disrupt the cell membrane of microbes, inhibit cellular division, and form biofilm in bacterial species, eventually reducing the number of microbes. Extraction of these compounds from the respective plants is carried out by different methods such as soxhlet, hydro-distillation, liquid-liquid extraction (LLE), pressurized liquid extraction (PLE), solid-phase extraction (SPE), supercritical fluid extraction (SFE), pulsed electric field (PEF), microwave-assisted extraction (MAE), enzyme-assisted extraction (EAE), ultrasound-assisted extraction (UAE), and high-voltage electrical discharge. Suitable selection of the extraction technique highly depends upon the associated advantages and disadvantages. In order to aid future study in this field, this review paper summarizes the advantages and disadvantages of each of these approaches. Additionally, the discussion covers how antimicrobial agents destroy harmful bacteria. Thus, this review offers in-depth knowledge to researchers on the antibacterial properties of Capsicum, Nigella sativa, Musa paradisiaca L. peels, and Citrus limetta.
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Awuku-Sowah EM, Graham NA, Watson NM. Investigating mangrove-human health relationships: A review of recently reported physiological benefits. DIALOGUES IN HEALTH 2022; 1:100059. [PMID: 38515922 PMCID: PMC10954016 DOI: 10.1016/j.dialog.2022.100059] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 08/10/2022] [Revised: 10/10/2022] [Accepted: 10/12/2022] [Indexed: 03/23/2024]
Abstract
Ecosystems continue to experience degradation worldwide, with diminishing ecosystem services presenting unfavourable outlooks for all aspects of human wellbeing including health. To inform protective policies that safeguard both ecological and health benefits, syntheses of available knowledge are required especially for neglected ecosystems such as mangroves. However, reviews about relationships between mangroves and human health are rare. This review identifies and categorizes evidence reported in the Web of Science database about health impacts of mangrove ecosystem goods and services. 96 papers were retained after application of exclusion criteria and filtration steps to results of database and bibliographical searches. Findings highlight most abundantly that bioactive extracts of mangrove sediment, plant, and plant associates are useful for the treatment of human ailments and infections. Also reported is the heavy and trace metal bioremediation capacity of mangroves ecosystems, with concomitant modulating effects on associated human health risks. Evidence of mangrove influence on human nutrition via fisheries and food production support services, either singularly or in conjunction with linked ecosystems is also offered. Finally, mangrove effects on the prevalence of causative agents, and therefore on the incidence and distribution of infectious diseases, are also presented. Positive influences of mangroves on human health are implied via three of the four routes reported, which diminish with degradation and appreciate with proper ecosystem functioning. The undesirable links lie chiefly with higher infectious disease risk posed by mangroves, which requires further exploration regarding suspected ecological pathways available for limiting said risks. Other gaps identified are sparse information about in-vivo efficacy and safety of mangrove bioactive isolates, specific nutrient content and diversity associated with mangrove-supported food production outcomes, and the geographically limited nature of most findings. Beyond economic value, health benefits of mangroves are significant and outweigh their disservices to humans. To ensure sustainable supply of the full complement of these benefits, they should be considered when designing ecosystems management regimes.
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Phenolic compounds classification and their distribution in winemaking by-products. Eur Food Res Technol 2022. [DOI: 10.1007/s00217-022-04163-z] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/27/2022]
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Hernández-Martínez MA, Suárez-Rodríguez LM, López-Meza JE, Ochoa-Zarzosa A, Salgado-Garciglia R, Fernández-Pavia SP, López-Gómez R. Antifungal Activity of Avocado Seed Recombinant GASA/Snakin PaSn. Antibiotics (Basel) 2022; 11:1558. [PMID: 36358213 PMCID: PMC9686948 DOI: 10.3390/antibiotics11111558] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/11/2022] [Revised: 11/01/2022] [Accepted: 11/02/2022] [Indexed: 06/15/2024] Open
Abstract
The avocado fruit (Persea americana) has become a significant fruit in the human diet for its nutritional properties. However, the seed is a source of bioactive molecules and has been poorly utilized. Previously, we reported that the PaSn gene is expressed in the avocado seeds, a cysteine-rich antimicrobial peptide (GASA/Snakin), and demonstrated its antibacterial activity. In this work, we report the recombinant production of PaSn in the Escherichia coli system and evaluate its antifungal activity against plant and human pathogen fungi. The recombinant peptide showed antifungal activity at 200 μg/mL against phytopathogens Colletotrichum gloeosporioides and Fusarium oxysporum and human pathogens Candida albicans and C. glabrata. Our results demonstrate the usefulness of a prokaryotic expression system for avocado antimicrobial peptide production. In conclusion, the snakin PaSn could be helpful in the control of postharvest avocado and other fruits' fungal diseases and human fungal pathogens.
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Affiliation(s)
| | - Luis María Suárez-Rodríguez
- Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Mexico
| | - Joel Edmundo López-Meza
- Centro Multidisciplinario de Estudios en Biotecnología, Universidad Michoacana de San Nicolás de Hidalgo, Tarimbaro 58893, Mexico
| | - Alejandra Ochoa-Zarzosa
- Centro Multidisciplinario de Estudios en Biotecnología, Universidad Michoacana de San Nicolás de Hidalgo, Tarimbaro 58893, Mexico
| | - Rafael Salgado-Garciglia
- Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Mexico
| | - Silvia Patricia Fernández-Pavia
- Instituto de Investigaciones Agropecuarias y Forestales, Universidad Michoacana de San Nicolás de Hidalgo, Tarimbaro 58893, Mexico
| | - Rodolfo López-Gómez
- Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, Mexico
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Saini J, Kaur P, Malik N, Lakhawat SS, Sharma PK. Antimicrobial peptides: A promising tool to combat multidrug resistance in SARS CoV2 era. Microbiol Res 2022; 265:127206. [PMID: 36162150 PMCID: PMC9491010 DOI: 10.1016/j.micres.2022.127206] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/06/2022] [Revised: 06/26/2022] [Accepted: 09/16/2022] [Indexed: 10/25/2022]
Abstract
COVID-19 (Coronavirus Disease 2019), a life-threatening viral infection, is caused by a highly pathogenic virus named SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2). Currently, no treatment is available for COVID-19; hence there is an urgent need to find effective therapeutic drugs to combat COVID-19 pandemic. Considering the fact that the world is facing a major issue of antimicrobial drug resistance, naturally occurring compounds have the potential to achieve this goal. Antimicrobial peptides (AMPs) are naturally occurring antimicrobial agents which are effective against a wide variety of microbial infections. Therefore, the use of AMPs is an attractive therapeutic strategy for the treatment of SARS-CoV-2 infection. This review sheds light on the potential of antimicrobial peptides as antiviral agents followed by a comprehensive description of effective antiviral peptides derived from various natural sources found to be effective against SARS-CoV and other respiratory viruses. It also highlights the mechanisms of action of antiviral peptides with special emphasis on their effectiveness against SARS-CoV-2 infection.
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Affiliation(s)
- Jasleen Saini
- Department of Biotechnology, Sri Guru Granth Sahib World University, Fatehgarh Sahib, Punjab, India
| | - Pritpal Kaur
- Department of Biotechnology, Sri Guru Granth Sahib World University, Fatehgarh Sahib, Punjab, India
| | - Naveen Malik
- Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, India
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Effects of Piper guineense (African Black Pepper) Seeds on Lipid Profile, Renal Function Parameters, and Antioxidant Status of Cavia porcellus. MEDICAL LABORATORY JOURNAL 2022. [DOI: 10.52547/mlj.16.5.3] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 03/06/2023] Open
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Medeiros CIS, Sousa MNAD, Filho GGA, Freitas FOR, Uchoa DPL, Nobre MSC, Bezerra ALD, Rolim LADMM, Morais AMB, Nogueira TBSS, Nogueira RBSS, Filho AAO, Lima EO. Antifungal activity of linalool against fluconazole-resistant clinical strains of vulvovaginal Candida albicans and its predictive mechanism of action. Braz J Med Biol Res 2022; 55:e11831. [PMID: 35976268 PMCID: PMC9377531 DOI: 10.1590/1414-431x2022e11831] [Citation(s) in RCA: 13] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/08/2022] [Accepted: 06/29/2022] [Indexed: 12/01/2022] Open
Abstract
Candida albicans is the most frequently isolated opportunistic
pathogen in the female genital tract, with 92.3% of cases in Brazil associated
with vulvovaginal candidiasis (VVC). Linalool is a monoterpene compound from
plants of the genera Cinnamomum, Coriandrum,
Lavandula, and Citrus that has demonstrated a
fungicidal effect on strains of Candida spp., but its mechanism
of action is still unknown. For this purpose, broth microdilution techniques
were applied, as well as molecular docking in a predictive manner for this
mechanism. The main results of this study indicated that the C.
albicans strains analyzed were resistant to fluconazole and
sensitive to linalool at a dose of 256 µg/mL. Furthermore, the increase in the
minimum inhibitory concentration (MIC) of linalool in the presence of sorbitol
and ergosterol indicated that this molecule possibly affects the cell wall and
plasma membrane integrity of C. albicans. Molecular docking of
linalool with proteins that are key in the biosynthesis and maintenance of the
cell wall and the fungal plasma membrane integrity demonstrated the possibility
of linalool interacting with three important enzymes: 1,3-β-glucan synthase,
lanosterol 14α-demethylase, and Δ 14-sterol reductase. In
silico analysis showed that this monoterpene has theoretical but
significant oral bioavailability, low toxic potential, and high similarity to
pharmaceuticals. Therefore, the findings of this study indicated that linalool
probably causes damage to the cell wall and plasma membrane of C.
albicans, possibly by interaction with important enzymes involved
in the biosynthesis of these fungal structures, in addition to presenting low
in silico toxic potential.
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Affiliation(s)
- C I S Medeiros
- Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, João Pessoa, PB, Brasil.,Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - M N A de Sousa
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - G G A Filho
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - F O R Freitas
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - D P L Uchoa
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - M S C Nobre
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - A L D Bezerra
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - L A D M M Rolim
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - A M B Morais
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - T B S S Nogueira
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - R B S S Nogueira
- Curso de Medicina, Centro Universitário UniFIP, Patos, PB, Brasil
| | - A A O Filho
- Unidade Acadêmica de Ciências Biológicas (UACB), Universidade Federal de Campina Grande, Patos, PB, Brasil
| | - E O Lima
- Departamento de Ciências Farmacêuticas, Universidade Federal da Paraíba, João Pessoa, PB, Brasil
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The Evaluation of Various Biological Properties for Bismuth Oxychloride Nanoparticles (BiOCl NPs). INORG CHEM COMMUN 2022. [DOI: 10.1016/j.inoche.2022.109850] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/23/2022]
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In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of Capsicum chinense By-Product Extracts. APPLIED SCIENCES-BASEL 2022. [DOI: 10.3390/app12125818] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
Abstract
Capsicum chinense J., also known locally as habanero pepper, is a medicinal herb known for its pharmacological properties. Its properties are attributed to the capsaicinoids and polyphenols found in its fruit and polyphenols in its by-products. The anticancer potential of C. chinense by-products remains unexplored. This study aimed to evaluate the antiproliferative activity and modulation of the cytotoxicity of extracts obtained from C. chinense by-products of plants grown on black and red soils of Yucatan, Mexico. Dry by-product extracts were obtained using maceration, a Soxhlet, and supercritical fluid extraction. In vitro antiproliferative activity and cytotoxicity modulation were evaluated by the sulforhodamine B method. The extract of leaves of plants grown on black soil obtained by maceration displayed selective high cytotoxicity against colorectal cancer cells, IC50 HCT–15 = 16.23 ± 2.89 µg mL−1. The leaf and stem extracts of plants grown on red soil obtained by maceration potentiated the vinblastine’s effect against parental breast cancer cells, MCF–7/Sens, with a reversion factor of 362.50-fold. Additionally, the extract of stems from plants grown on black soil obtained by supercritical fluid extraction and all the by-product extracts from plants grown on black soil obtained through maceration increased the effect of vinblastine against MCF–7/Vin+ with a reversion factor from 5.06- to 7.78-fold. These results highlight the anticancer potential of C. chinense by-products.
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García Beltrán JM, Esteban MÁ. Nature-identical compounds as feed additives in aquaculture. FISH & SHELLFISH IMMUNOLOGY 2022; 123:409-416. [PMID: 35331881 DOI: 10.1016/j.fsi.2022.03.010] [Citation(s) in RCA: 12] [Impact Index Per Article: 4.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 11/30/2021] [Revised: 03/11/2022] [Accepted: 03/15/2022] [Indexed: 06/14/2023]
Abstract
Aquaculture sustainable development is necessary since it is categorized as the most important source of aquatic products for human consumption and it is expected to keep growing shortly. For this reason, the addition of natural immunostimulants to fish diet to improve fish health and to preserve the environment have great importance in aquaculture. In this sense, new biotechnological tools as nature-identical compounds are now being used as feed additives to strengthen and stimulate the fish immune system to prevent and/or control diseases due to their lesser cost and higher availability than plant compounds. This review aims to present the most recent studies in which nature-identical compounds have been used in the fish diet to establish their possible use in aquaculture. Nature-identical compounds can be considered a promising alternative to be added to fish diets to promote growth performance, manipulate the gut microbiota, and improve the immune and oxidative status of fish as wells as control bacterial infections in this important aquatic industry.
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Affiliation(s)
- José María García Beltrán
- Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain
| | - María Ángeles Esteban
- Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
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Sagar PK, Sharma P, Singh R. Inhibition of Quorum Sensing Regulated Virulence Factors and Biofilm Formation by Eucalyptus globulus against Multidrug-Resistant Pseudomonas aeruginosa. J Pharmacopuncture 2022; 25:37-45. [PMID: 35371582 PMCID: PMC8947967 DOI: 10.3831/kpi.2022.25.1.37] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/12/2020] [Revised: 12/28/2020] [Accepted: 12/13/2021] [Indexed: 11/18/2022] Open
Abstract
Objectives The quorum-sensing–inhibitory and anti-biofilm activities of the methanol extract of E. globulus leaves were determined against clinically isolated multidrug-resistant Pseudomonas aeruginosa. Methods The preliminary anti-quorum–sensing (AQS) activity of eucalyptus was investigated against a biosensor strain Chromobacterium violaceum ATCC 12472 (CV12472) by using the agar well diffusion method. The effect of sub-minimum inhibitory concentrations (sub-MICs) of the methanol extract of eucalyptus on different quorum-sensing–regulated virulence factors, such as swarming motility, pyocyanin pigment, exopolysaccharide (EPS), and biofilm formation, against clinical isolates (CIs 2, 3, and 4) and reference PA01 of Pseudomonas aeruginosa were determined using the swarm diameter (mm)-measurement method, chloroform extraction method, phenol (5%)-sulphuric acid (concentrated) method, and the microtiter plate assay respectively, and the inhibition (%) in formation were calculated. Results The preliminary AQS activity (violacein pigment inhibition) of eucalyptus was confirmed against Chromobacterium violaceum ATCC 12472 (CV12472). The eucalyptus extract also showed concentration-dependent inhibition (%) of swarming motility, pyocyanin pigment, EPS, and biofilm formation in different CIs and PA01 of P. aeruginosa. Conclusion Our results revealed the effectiveness of the E. globulus extract for the regulation of quorum-sensing–dependent virulence factors and biofilm formation at a reduced dose (sub-MICs) and suggest that E. globulus may be a therapeutic agent for curing and controlling bacterial infection and thereby reducing the possibility of resistance development in pathogenic strains.
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Affiliation(s)
- Pankaj Kumar Sagar
- Department of Biotechnology, Bundelkhand University, Jhansi (U.P.), India
| | - Poonam Sharma
- Department of Zoology, Indira Gandhi National Tribal University (A Central University), Amarkantak, Madhya Pradesh, India
| | - Rambir Singh
- Department of Horticulture, Aromatic and Medicinal Plants, Mizoram University (A Central University), Aizawl, Mizoram, India
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Strieth D, Lenz S, Ulber R. In vivo and in silico screening for antimicrobial compounds from cyanobacteria. Microbiologyopen 2022; 11:e1268. [PMID: 35478288 PMCID: PMC8924698 DOI: 10.1002/mbo3.1268] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/16/2021] [Revised: 02/07/2022] [Accepted: 02/07/2022] [Indexed: 12/11/2022] Open
Abstract
Due to the emerging rise of multi‐drug resistant bacteria, the discovery of novel antibiotics is of high scientific interest. Through their high chemodiversity of bioactive secondary metabolites, cyanobacteria have proven to be promising microorganisms for the discovery of antibacterial compounds. These aspects make appropriate antibacterial screening approaches for cyanobacteria crucial. Up to date, screenings are mostly carried out using a phenotypic methodology, consisting of cyanobacterial cultivation, extraction, and inhibitory assays. However, the parameters of these methods highly vary within the literature. Therefore, the common choices of parameters and inhibitory assays are summarized in this review. Nevertheless, less frequently used method variants are highlighted, which lead to hits from antimicrobial compounds. In addition to the considerations of phenotypic methods, this study provides an overview of developments in the genome‐based screening area, be it in vivo using PCR technique or in silico using the recent genome‐mining method. Though, up to date, these techniques are not applied as much as phenotypic screening.
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Affiliation(s)
- Dorina Strieth
- Chair of Bioprocess Engineering University of Kaiserslautern Kaiserslautern Germany
| | - Selina Lenz
- Chair of Bioprocess Engineering University of Kaiserslautern Kaiserslautern Germany
| | - Roland Ulber
- Chair of Bioprocess Engineering University of Kaiserslautern Kaiserslautern Germany
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Diniz-Neto H, Silva SL, Cordeiro LV, Silva DF, Oliveira RF, Athayde-Filho PF, Oliveira-Filho AA, Guerra FQS, Lima EO. Antifungal activity of 2-chloro-N-phenylacetamide: a new molecule with fungicidal and antibiofilm activity against fluconazole-resistant Candida spp. BRAZ J BIOL 2022; 84:e255080. [PMID: 35262564 DOI: 10.1590/1519-6984.255080] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/05/2021] [Accepted: 01/19/2022] [Indexed: 11/22/2022] Open
Abstract
In the current context of emerging drug-resistant fungal pathogens such as Candida albicans and Candida parapsilosis, discovery of new antifungal agents is an urgent matter. This research aimed to evaluate the antifungal potential of 2-chloro-N-phenylacetamide against fluconazole-resistant clinical strains of C. albicans and C. parapsilosis. The antifungal activity of 2-chloro-N-phenylacetamide was evaluated in vitro by the determination of the minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), inhibition of biofilm formation and its rupture, sorbitol and ergosterol assays, and association between this molecule and common antifungal drugs, amphotericin B and fluconazole. The test product inhibited all strains of C. albicans and C. parapsilosis, with a MIC ranging from 128 to 256 µg.mL-1, and a MFC of 512-1,024 µg.mL-1. It also inhibited up to 92% of biofilm formation and rupture of up to 87% of preformed biofilm. 2-chloro-N-phenylacetamide did not promote antifungal activity through binding to cellular membrane ergosterol nor it damages the fungal cell wall. Antagonism was observed when combining this substance with amphotericin B and fluconazole. The substance exhibited significant antifungal activity by inhibiting both planktonic cells and biofilm of fluconazole-resistant strains. Its combination with other antifungals should be avoided and its mechanism of action remains to be established.
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Affiliation(s)
- H Diniz-Neto
- Universidade Federal da Paraíba - UFPB, Department of Pharmaceutical Sciences - DCF, João Pessoa, PB, Brasil
| | - S L Silva
- Universidade Federal da Paraíba - UFPB, Department of Pharmaceutical Sciences - DCF, João Pessoa, PB, Brasil
| | - L V Cordeiro
- Universidade Federal da Paraíba - UFPB, Department of Pharmaceutical Sciences - DCF, João Pessoa, PB, Brasil
| | - D F Silva
- Universidade Federal da Paraíba - UFPB, Department of Pharmaceutical Sciences - DCF, João Pessoa, PB, Brasil
| | - R F Oliveira
- Universidade Federal da Paraíba - UFPB, Department of Chemistry - DQ, João Pessoa, PB, Brasil
| | - P F Athayde-Filho
- Universidade Federal da Paraíba - UFPB, Department of Chemistry - DQ, João Pessoa, PB, Brasil
| | - A A Oliveira-Filho
- Universidade Federal de Campina Grande - UFCG, Rural Health and Technology Center - CTSR, Patos, PB, Brasil
| | - F Q S Guerra
- Universidade Federal da Paraíba - UFPB, Department of Pharmaceutical Sciences - DCF, João Pessoa, PB, Brasil
| | - E O Lima
- Universidade Federal da Paraíba - UFPB, Department of Pharmaceutical Sciences - DCF, João Pessoa, PB, Brasil
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Abuga I, Sulaiman SF, Abdul Wahab R, Ooi KL, Abdull Rasad MSB. Phytochemical constituents and antibacterial activities of 45 Malay traditional medicinal plants. J Herb Med 2022. [DOI: 10.1016/j.hermed.2021.100496] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 01/09/2023]
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50
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Cell Culture-Based Assessment of Toxicity and Therapeutics of Phytochemical Antioxidants. Molecules 2022; 27:molecules27031087. [PMID: 35164354 PMCID: PMC8839249 DOI: 10.3390/molecules27031087] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/04/2022] [Revised: 01/31/2022] [Accepted: 02/03/2022] [Indexed: 12/13/2022] Open
Abstract
Plant-derived natural products are significant resources for drug discovery and development including appreciable potentials in preventing and managing oxidative stress, making them promising candidates in cancer and other disease therapeutics. Their effects have been linked to phytochemicals such as phenolic compounds and their antioxidant activities. The abundance and complexity of these bio-constituents highlight the need for well-defined in vitro characterization and quantification of the plant extracts/preparations that can translate to in vivo effects and hopefully to clinical use. This review article seeks to provide relevant information about the applicability of cell-based assays in assessing anti-cytotoxicity of phytochemicals considering several traditional and current methods.
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