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Demirel S. Vasorelaxant effects of biochemical constituents of various medicinal plants and their benefits in diabetes. World J Diabetes 2024; 15:1122-1141. [PMID: 38983824 PMCID: PMC11229960 DOI: 10.4239/wjd.v15.i6.1122] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/30/2023] [Revised: 03/07/2024] [Accepted: 05/06/2024] [Indexed: 06/11/2024] Open
Abstract
Endothelial function plays a pivotal role in cardiovascular health, and dysfunction in this context diminishes vasorelaxation concomitant with endothelial activity. The nitric oxide-cyclic guanosine monophosphate pathway, prostacyclin-cyclic adenosine monophosphate pathway, inhibition of phosphodiesterase, and the opening of potassium channels, coupled with the reduction of calcium levels in the cell, constitute critical mechanisms governing vasorelaxation. Cardiovascular disease stands as a significant contributor to morbidity and mortality among individuals with diabetes, with adults afflicted by diabetes exhibiting a heightened cardiovascular risk compared to their non-diabetic counterparts. A plethora of medicinal plants, characterized by potent pharmacological effects and minimal side effects, holds promise in addressing these concerns. In this review, we delineate various medicinal plants and their respective biochemical constituents, showcasing concurrent vasorelaxant and anti-diabetic activities.
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Affiliation(s)
- Sadettin Demirel
- Medicine School, Physiology Department, Bursa Uludag University, Bursa 16059, Türkiye
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Ju SM, Ali MY, Ko SM, Ryu JH, Choi JS, Jung HA. An Arylbenzofuran, Stilbene Dimers, and Prenylated Diels–Alder Adducts as Potent Diabetic Inhibitors from Morus bombycis Leaves. Antioxidants (Basel) 2023; 12:antiox12040837. [PMID: 37107213 PMCID: PMC10134988 DOI: 10.3390/antiox12040837] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/10/2023] [Revised: 03/27/2023] [Accepted: 03/28/2023] [Indexed: 04/01/2023] Open
Abstract
Morus bombycis has a long history of usage as a treatment for metabolic diseases, especially, diabetes mellitus (DM). Thus, we aimed to isolate and evaluate bioactive constituents derived from M. bombycis leaves for the treatment of DM. According to bioassay-guided isolation by column chromatography, eight compounds were obtained from M. bombycis leaves: two phenolic compounds, p-coumaric acid (1) and chlorogenic acid methyl ester (2), one stilbene, oxyresveratrol (3), two stilbene dimers, macrourin B (4) and austrafuran C (6), one 2-arylbenzofuran, moracin M (5), and two Diels–Alder type adducts, mulberrofuran F (7) and chalcomoracin (8). Among the eight isolated compounds, the anti-DM activity of 3–8 (which possess chemotaxonomic significance in Morus species) was evaluated by inhibition of α-glucosidase, protein tyrosine phosphatase 1B (PTP1B), human recombinant aldose reductase (HRAR), and advanced glycation end-product (AGE) formation as well as by scavenging peroxynitrite (ONOO−), which are crucial therapeutic targets of DM and its complications. Compounds 4 and 6–8 significantly inhibited α-glucosidase, PTP1B, and HRAR enzymes with mixed-type and non-competitive-type inhibition modes. Furthermore, the four compounds had low negative binding energies in both enzymes according to molecular docking simulation, and compounds 3–8 exhibited strong antioxidant capacity by inhibiting AGE formation and ONOO− scavenging. Overall results suggested that the most active stilbene-dimer-type compounds (4 and 6) along with Diels–Alder type adducts (7 and 8) could be promising therapeutic and preventive resources against DM and have the potential to be used as antioxidants, anti-diabetic agents, and anti-diabetic complication agents.
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Affiliation(s)
- Seon Min Ju
- Department of Food Science and Human Nutrition, Jeonbuk National University, Jeonju 54896, Republic of Korea
| | - Md Yousof Ali
- Department of Food Science and Nutrition, Pukyong National University, Busan 48547, Republic of Korea
- Department of Clinical Neurosciences, Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N4N1, Canada
| | - Seung-Mi Ko
- Department of Food Science and Human Nutrition, Jeonbuk National University, Jeonju 54896, Republic of Korea
| | - Jung-Hye Ryu
- Department of Food Science and Human Nutrition, Jeonbuk National University, Jeonju 54896, Republic of Korea
| | - Jae-Sue Choi
- Department of Food Science and Nutrition, Pukyong National University, Busan 48547, Republic of Korea
- Correspondence: (J.-S.C.); (H.-A.J.); Tel.: +82-51-629-5845 (J.-S.C.); +82-63-270-4882 (H.-A.J.)
| | - Hyun-Ah Jung
- Department of Food Science and Human Nutrition, Jeonbuk National University, Jeonju 54896, Republic of Korea
- Correspondence: (J.-S.C.); (H.-A.J.); Tel.: +82-51-629-5845 (J.-S.C.); +82-63-270-4882 (H.-A.J.)
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Karigidi KO, Olaiya CO. Antidiabetic activity of corn steep liquor extract of Curculigo pilosa and its solvent fractions in streptozotocin-induced diabetic rats. J Tradit Complement Med 2020; 10:555-564. [PMID: 33134131 PMCID: PMC7588337 DOI: 10.1016/j.jtcme.2019.06.005] [Citation(s) in RCA: 17] [Impact Index Per Article: 3.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/12/2018] [Revised: 06/16/2019] [Accepted: 06/23/2019] [Indexed: 01/23/2023] Open
Abstract
The rhizome of Curculigo pilosa (CP) prepared with Corn steep liquor (CSL), is traditionally used in the treatment of diabetes mellitus. In this study, antidiabetic activity of the CSL extract and its fractions (butanol and methanol) were evaluated in streptozotocin-induced diabetic rats. Diabetes mellitus was induced by single intraperitoneal administration of streptozotocin (50 mg/kg) and diabetic rats were treated with 300 mg/kg bodyweight of the extract(s) for 28 days. Antidiabetic effect was monitored by plasma blood glucose, oral glucose tolerances test (OGGT), body weight and heamatological indices. Also serum urea, creatinine, cholesterol, high density lipoprotein, bilirubin, alkaline phosphatase, aspartate and alanine transaminases were evaluated. The levels of hepatic glutathione, catalase, superoxide dismutase, lipid peroxidation, nitric oxide and hydrogen peroxide were assessed; also histopathology of the hepatic tissues was examined. Oral administration of the extract resulted in significant (p < 0.05) reduction of plasma blood glucose (29.32% crude extract and 22.96% butanol fraction) and also increased body weight (20.61% crude extract, 13.44% butanol fraction and 6.23% methanol fraction) of diabetic rats. The heamotogical indices, plasma parameters and hepatic oxidative stress in diabetic rats were returned to near normalcy following treatment with the extract(s). The GC-MS analysis of the extract revealed the presence of stilbene, a proven antidiabetic agent, which might be responsible for the antidiabetic activity. The results obtained suggest that the CSL extract of CP could be used in management of diabetes mellitus thus providing scientific validation of its use as an antidiabetic agent.
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Affiliation(s)
- Kayode Olayele Karigidi
- Nutritional and Industrial Biochemistry Research Laboratories, Department of Biochemistry, University of Ibadan, Nigeria
- Department of Chemical Sciences, Ondo State University of Science and Technology, Okitipupa, Nigeria
| | - Charles Ojo Olaiya
- Nutritional and Industrial Biochemistry Research Laboratories, Department of Biochemistry, University of Ibadan, Nigeria
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Apaya MK, Kuo TF, Yang MT, Yang G, Hsiao CL, Chang SB, Lin Y, Yang WC. Phytochemicals as modulators of β-cells and immunity for the therapy of type 1 diabetes: Recent discoveries in pharmacological mechanisms and clinical potential. Pharmacol Res 2020; 156:104754. [DOI: 10.1016/j.phrs.2020.104754] [Citation(s) in RCA: 15] [Impact Index Per Article: 3.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/07/2019] [Revised: 03/07/2020] [Accepted: 03/10/2020] [Indexed: 12/19/2022]
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Jeon YH, Choi SW. Isolation, Identification, and Quantification of Tyrosinase and α-Glucosidase Inhibitors from UVC-Irradiated Mulberry ( Morus alba L.) Leaves. Prev Nutr Food Sci 2018; 24:84-94. [PMID: 31008101 PMCID: PMC6456241 DOI: 10.3746/pnf.2019.24.1.84] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/31/2019] [Accepted: 12/06/2018] [Indexed: 11/28/2022] Open
Abstract
Methanol extracts from ultraviolet (UV) C-irradiated mulberry leaves (UVC-IML) exhibit stronger tyrosinase and α-glucosidase inhibitory activities than those from unirradiated mulberry leaves. Through a bioassay-guided fractionation and purification process, two oxyresveratrol derivatives, oxyresveratrol (ORT) and 4′-prenyloxyresveratrol (PORT), and six 2-arylbenzofuran derivatives [moracin B (MCB), moracin C (MCC), moracin M (MCM), moracin N (MCN), 6,5′-dimethoxymoracin M (DMMCM), and chalcomoracin (CMC)] were isolated from the methanol extract from UVC-IML. Their chemical structures were determined by UV, mass, and nuclear magnetic resonance spectrometry. ORT and PORT showed potent tyrosinase inhibitory activities with the half maximal inhibitory concentration (IC50) values of 0.57 and 0.90 μM, respectively, and CMC exhibited significant tyrosinase and α-glucosidase inhibitory activity with IC50 values of 5.61 and 6.00 μM, respectively. Levels of these eight compounds were increased significantly following irradiation compared with untreated mulberry leaves; ORTs increased approximately 4 fold and moracins increased 2~16 fold. These data suggest that UVC-IML may represent a promising source of nutraceuticals and cosmeceuticals for prevention of diabetes and skin aging.
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Affiliation(s)
- Young-Hee Jeon
- Department of Food Science and Nutrition, Daegu Catholic University, Gyeongbuk 61848, Korea
| | - Sang-Won Choi
- Department of Food Science and Nutrition, Daegu Catholic University, Gyeongbuk 61848, Korea
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Shim SY, Sung SH, Lee M. Anti-inflammatory activity of mulberrofuran K isolated from the bark of Morus bombycis. Int Immunopharmacol 2018; 58:117-124. [PMID: 29573719 DOI: 10.1016/j.intimp.2017.11.002] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/08/2017] [Revised: 11/02/2017] [Accepted: 11/02/2017] [Indexed: 12/24/2022]
Abstract
Morus bombycis Koidzumi, commonly known as silkworm mulberry, is a plant belonging to family Moraceae. It has been used in Asian countries as a traditional medicine for treating hypertension, diabetes, and inflammatory disorders. In this study, we isolated eleven compounds from the cortex of M. bombycis and evaluated their inhibitory effects on nitric oxide (NO) production as an indicator of their anti-inflammatory activities using lipopolysaccharide (LPS)-stimulated murine macrophages. Compound 4 showed the most potent inhibitory activity on NO production. It was identified as mulberrofuran K (MFK). Anti-inflammatory activity of MFK was then carried out using LPS-stimulated RAW264.7 cells. MFK suppressed the production of NO, reactive oxygen species (ROS), and proinflammatory cytokines (interleukin (IL)-1β, IL-6 and tumor necrosis factor alpha (TNF-α)) in a concentration-dependent manner. Western blot analysis revealed that MFK treatment inhibited expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). MFK also inhibited transcriptional activation of nuclear factor-κB (NF-κB) and extracellular-regulated kinases (ERK) 1/2 in LPS-stimulated murine macrophages. These results suggest that MFK, an anti-inflammatory constituents of M. bombycis cortex, has potential as a therapeutic candidates for preventing and treating inflammatory diseases.
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Affiliation(s)
- Sun-Yup Shim
- College of Pharmacy, Sunchon National University, 255 Jungangno, Suncheon-si 57922, Jeonnam, Republic of Korea; Research Institute of Life and Pharmaceutical Sciences, 255 Jungangno, Suncheon-si 57922, Jeonnam, Republic of Korea
| | - Sang Hyun Sung
- College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
| | - Mina Lee
- College of Pharmacy, Sunchon National University, 255 Jungangno, Suncheon-si 57922, Jeonnam, Republic of Korea; Suncheon Research Center for Natural Medicines, 255 Jungangno, Suncheon-si 57922, Jeonnam, Republic of Korea.
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Wei H, Zhu JJ, Liu XQ, Feng WH, Wang ZM, Yan LH. Review of bioactive compounds from root barks of Morus plants (Sang-Bai-Pi) and their pharmacological effects. ACTA ACUST UNITED AC 2016. [DOI: 10.1080/23312009.2016.1212320] [Citation(s) in RCA: 24] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/21/2022]
Affiliation(s)
- Hua Wei
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China
- Key Laboratory of Plant Resources Conservation and Utilization, Jishou University, College of Hunan Province, 416000 Jishou, China
| | - Jing-Jing Zhu
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China
| | - Xiao-Qian Liu
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China
| | - Wei-Hong Feng
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China
| | - Zhi-Min Wang
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China
| | - Li-Hua Yan
- Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China
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Zhao S, Park CH, Li X, Kim YB, Yang J, Sung GB, Park NI, Kim S, Park SU. Accumulation of Rutin and Betulinic Acid and Expression of Phenylpropanoid and Triterpenoid Biosynthetic Genes in Mulberry (Morus alba L.). JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2015; 63:8622-8630. [PMID: 26343778 DOI: 10.1021/acs.jafc.5b03221] [Citation(s) in RCA: 33] [Impact Index Per Article: 3.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 06/05/2023]
Abstract
Mulberry (Morus alba L.) is used in traditional Chinese medicine and is the sole food source of the silkworm. Here, 21 cDNAs encoding phenylpropanoid biosynthetic genes and 21 cDNAs encoding triterpene biosynthetic genes were isolated from mulberry. The expression levels of genes involved in these biosynthetic pathways and the accumulation of rutin, betulin, and betulinic acid, important secondary metabolites, were investigated in different plant organs. Most phenylpropanoid and triterpene biosynthetic genes were highly expressed in leaves and/or fruit, and most genes were downregulated during fruit ripening. The accumulation of rutin was more than fivefold higher in leaves than in other organs, and higher levels of betulin and betulinic acid were found in roots and leaves than in fruit. By comparing the contents of these compounds with gene expression levels, we speculate that MaUGT78D1 and MaLUS play important regulatory roles in the rutin and betulin biosynthetic pathways.
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Affiliation(s)
- Shicheng Zhao
- Department of Crop Science, Chungnam National University , 99 Daehak-Ro, Yuseong-gu, Daejeon 305-764, Korea
| | - Chang Ha Park
- Department of Crop Science, Chungnam National University , 99 Daehak-Ro, Yuseong-gu, Daejeon 305-764, Korea
| | - Xiaohua Li
- Department of Crop Science, Chungnam National University , 99 Daehak-Ro, Yuseong-gu, Daejeon 305-764, Korea
| | - Yeon Bok Kim
- Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration , Bisanro 92, Eumseong, Chungbuk, 369-873, Korea
| | - Jingli Yang
- State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University , 26 Hexing Road, Harbin 150040, China
| | - Gyoo Byung Sung
- Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration , Wanju 565-851, Korea
| | - Nam Il Park
- Deptartment of Plant Science, Gangneung-Wonju National University 7 Jukheon-gil , Gangneung-si, Gangwon-do 210-702, Korea
| | - Soonok Kim
- Biological and Genetic Resources Assessment Division, National Institute of Biological Resources , Incheon 404-170, Korea
| | - Sang Un Park
- Department of Crop Science, Chungnam National University , 99 Daehak-Ro, Yuseong-gu, Daejeon 305-764, Korea
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Wang S, Liu XM, Zhang J, Zhang YQ. An efficient preparation of mulberroside a from the branch bark of mulberry and its effect on the inhibition of tyrosinase activity. PLoS One 2014; 9:e109396. [PMID: 25299075 PMCID: PMC4192315 DOI: 10.1371/journal.pone.0109396] [Citation(s) in RCA: 19] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/24/2014] [Accepted: 09/03/2014] [Indexed: 11/18/2022] Open
Abstract
A bioactive ingredient in an ethanol extract from the branch bark of cultivated mulberry Husang-32 (Morus multicaulis Perr.) was isolated using a macroporous resin column. The primary component, which was purified by semi-preparative high-performance liquid chromatography diode array detection (HPLC-DAD), was identified as mulberroside A (MA) by liquid chromatograph-mass spectrometer (LC-MS), 1H and 13C nuclear magnetic resonance (NMR) spectra. In total, 4.12 g MA was efficiently extracted from one kilogram of mulberry bark. The enzymatic analysis showed that MA inhibited the generation of dopachrome by affecting the activities of monophenolase and diphenolase of tyrosinase in vitro. This analysis indicated that MA and oxyresveratrol (OR), which is the the aglycone of mulberroside A, exhibited strong inhibition of the monophenolase activity with IC50 values of 1.29 µmol/L and 0.12 µmol/L, respectively. However, the former showed weaker inhibitory activity than the latter for diphenolase. For the monophenolase activity, the inhibitory activity of MA and OR was reversible and showed mixed type 1 inhibition. Additionally, the inhibition constant KI (the inhibition constant of the effectors on tyrosinase) values were 0.385 µmol/L and 0.926 µmol/L, respectively, and the KIS (the inhibition constants of the enzyme-substrate complex) values were 0.177 µmol/L and 0.662 µmol/L, respectively. However, MA showed competitive inhibition of diphenolase activity, and KI was 4.36 µmol/L. In contrast, OR showed noncompetitive inhibition and KI = KIS = 2.95 µmol/L. Taken together, these results provide important information concerning the inhibitory mechanism of MA on melanin synthesis, which is widely used in whitening cosmetics.
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Affiliation(s)
- Shu Wang
- Silk Biotechnology Laboratory, School of Basic Medical and Biological Sciences, Soochow University, Dushuhu Higher Edu. Town, Suzhou, P R China
| | - Xian-Ming Liu
- Silk Biotechnology Laboratory, School of Basic Medical and Biological Sciences, Soochow University, Dushuhu Higher Edu. Town, Suzhou, P R China
| | - Jian Zhang
- College of Pharmaceutical Sciences, Soochow University, Dushuhu Higher Edu. Town, Suzhou, P R China
- * E-mail: (JZ); (YQZ)
| | - Yu-Qing Zhang
- Silk Biotechnology Laboratory, School of Basic Medical and Biological Sciences, Soochow University, Dushuhu Higher Edu. Town, Suzhou, P R China
- * E-mail: (JZ); (YQZ)
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Hu W, Yeo JH, Jiang Y, Heo SI, Wang MH. The antidiabetic effects of an herbal formula composed of Alnus hirsuta, Rosa davurica, Acanthopanax senticosus and Panax schinseng in the streptozotocin-induced diabetic rats. Nutr Res Pract 2013; 7:103-8. [PMID: 23610602 PMCID: PMC3627926 DOI: 10.4162/nrp.2013.7.2.103] [Citation(s) in RCA: 7] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/09/2012] [Revised: 12/16/2012] [Accepted: 01/04/2013] [Indexed: 11/05/2022] Open
Abstract
A folk prescription consisting of Alnus hirsuta, Rosa davurica, Acanthopanax senticosus and Panax schinseng has been used in the treatment of diabetes mellitus. The aim of the present investigation was to evaluate the antidiabetic effects of the herb formula extract (HFE) composed of Alnus hirsuta, Rosa davurica, Acanthopanax senticosus and Panax schinseng in the streptozotocin (STZ)-induced diabetic rats. The HFE was mixed in the food supply of the healthy and STZ-induced diabetic male Sprague-Dawley rats, and its effects on the body weight, water and food intake, hyperglycemia, hypolipidemic and islet structure were studied. The treatment of the rats with STZ for 6 weeks resulted in marasmus, polydipsia, polyphagia, hyperglycemia and hypoinsulinemia. In addition, the diabetic rats showed an apparent decrease in the insulin immunoreactivity and the number of β-cells in the pancreas. The addition of the HFE to the rats' food supply significantly lowered the serum glucose and the serum triglycerides level and preserved the normal histological appearance of the pancreatic islets. These results indicate that the HEF have a strong antidiabetic potential along with the significant hypoglycemic and hypolipidemic effects, which may be applicable in the pharmaceutical industry.
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Affiliation(s)
- Weicheng Hu
- Department of Medical Biotechnology, College of Biomedical Science, Kangwon National University, 192-1 Hyoja-dong, Chuncheon, Gangwon 200-701, Korea
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Kim HS, Kim AR, Park HJ, Park DK, Kim DK, Ko NY, Kim B, Choi DK, Won HS, Shin WS, Kim YM, Choi WS. Morus bombycis Koidzumi extract suppresses collagen-induced arthritis by inhibiting the activation of nuclear factor-κB and activator protein-1 in mice. JOURNAL OF ETHNOPHARMACOLOGY 2011; 136:392-398. [PMID: 21251971 DOI: 10.1016/j.jep.2011.01.016] [Citation(s) in RCA: 31] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 08/05/2010] [Revised: 10/08/2010] [Accepted: 01/10/2011] [Indexed: 05/30/2023]
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE Morus bombycis Koidzumi is widely distributed in Asia. In Korea, it has been used in traditional medicine because of its apparent anti-inflammatory, antioxidant, and hepatoprotective properties. AIM OF THE STUDY Although the extract of Morus bombycis Koidzumi (MB) has long since been used as a traditional anti-inflammatory medicine in Korea, its effect on arthritis remains unknown. We aimed to investigate the anti-arthritis activity of MB and the mechanism underlying it. MATERIALS AND METHODS The anti-arthritis activity of MB was assessed by using mouse models of type II collagen-induced arthritis (CIA). The clinical arthritis index and histopathological changes were evaluated in mice. Reverse transcriptase-polymerase chain reaction (RT-PCR), electrophoretic mobility shift assay (EMSA), and other biologic approaches were used for measuring the effect of MB on arthritis and understanding the underlying mechanism. RESULTS MB significantly decreased the clinical arthritis index in CIA mice; this was confirmed by examining histological changes in joints. Infiltration of immune cells, synovial hyperplasia, cartilage destruction, and bone erosion in the hind paw were largely suppressed by MB. The mRNA levels of matrix metalloproteinase (MMP)-1/MMP-3, inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6), and chemokines (macrophage inflammatory protein (MIP)-1, monocyte chemoattractant protein (MCP)-1, RANTES) were significantly suppressed by MB in a dose-dependent manner. The number of osteoclasts in the hind tibia was also significantly decreased. With regard to the mechanism, MB suppressed the activation of nuclear factor (NF)-κB and activator protein (AP)-1 in CIA mice. CONCLUSIONS MB produced an anti-arthritis effect in CIA mice by inhibiting the production of critical inflammatory mediators and osteoclasts through the downregulation of NF-κB and AP-1.
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Affiliation(s)
- Hyuk Soon Kim
- Department of Immunology and Physiology, College of Medicine, Konkuk University, Chungju, Republic of Korea
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Kim YS, Lee YM, Kim H, Kim J, Jang DS, Kim JH, Kim JS. Anti-obesity effect of Morus bombycis root extract: anti-lipase activity and lipolytic effect. JOURNAL OF ETHNOPHARMACOLOGY 2010; 130:621-624. [PMID: 20669373 DOI: 10.1016/j.jep.2010.05.053] [Citation(s) in RCA: 91] [Impact Index Per Article: 6.1] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 05/29/2023]
Abstract
AIM OF THE STUDY This study evaluated anti-obesity effect of the ethanolic extract of Morus bombycis root on lipase activity and lipolysis in adipocytes and adipose tissues. MATERIALS AND METHODS Lipase (triacylgycerol acylhydrolase, EC 3.1.1.3) activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Lipolytic effects were assayed in fully differentiated 3T3-L1 adipocytes and adipose tissues. In vitro, phosphodiesterase (PDE) activity was also measured. RESULTS Morus bombycis root extract exhibited strong anti-lipase activity, with an IC50 value of 2.07 microg/mL.In differentiated adipocytes and adipose tissues, the extract increased lipolytic effects such as decreased intracellular triglyceride and the release of glycerol. Further, the extract inhibited PDE activity in a dose-dependent manner. CONCLUSION The present study suggests that Morus bombycis root extract might be of therapeutic interest with respect to the treatment of obesity.
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Affiliation(s)
- Young Sook Kim
- Diabetic Complications Research Center, Division of Traditional Korean Medicine (TKM) Integrated Research, Korea Institute of Oriental Medicine (KIOM), Yuseong-gu, Daejeon, Republic of Korea
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Yuan HD, Jin GZ, Piao GC. Hepatoprotective effects of an active part from Artemisia sacrorum Ledeb. against acetaminophen-induced toxicity in mice. JOURNAL OF ETHNOPHARMACOLOGY 2010; 127:528-533. [PMID: 19833181 DOI: 10.1016/j.jep.2009.10.002] [Citation(s) in RCA: 37] [Impact Index Per Article: 2.5] [Reference Citation Analysis] [Abstract] [MESH Headings] [Track Full Text] [Subscribe] [Scholar Register] [Received: 04/07/2009] [Revised: 09/19/2009] [Accepted: 10/05/2009] [Indexed: 05/28/2023]
Abstract
AIMS OF STUDY Although Artemisia sacrorum Ledeb. (Compositae) has long been used as one kind of oriental folk medicine to treat some liver diseases, the underlying mechanism(s) by which these effects are induced remains to be defined. This study was designed to investigate the hepatoprotective effects of 50% ethanol eluate precipitation of Artemisia sacrorum Ledeb. (EEP) on acetaminophen (APAP)-induced toxicity in mice. MATERIALS AND METHODS The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and tumor necrosis factor-alpha (TNF-alpha) levels in mouse sera, and glutathione (GSH), malondialdehyde (MDA) in mouse liver tissues were measured. In addition, apoptosis and necrosis were evaluated by liver histopathological analysis and DNA laddering. Moreover, caspase-3 and -8 protein expressions in mouse livers were observed by Western blot analysis. RESULTS Pretreated with EEP prior to the administration of APAP significantly prevented the increases of AST, ALT, and TNF-alpha levels in sera, and suppressed the GSH depletion, MDA accumulation in liver tissues markedly. In addition, EEP prevented APAP-induced apoptosis and necrosis, as indicated by liver histopathological analysis, immunohistochemical analysis, and DNA laddering. Furthermore, according to the results from Western blot analysis, EEP decreased APAP-induced caspase-3 and caspase-8 protein expressions in mouse livers markedly. CONCLUSION All these results suggest that the protective effects of EEP against APAP-induced liver injury may involve mechanisms associated with its inhibitive effects of lipid peroxidation and the down-regulation of TNF-alpha mediated apoptosis. In a word, EEP could be a valuable candidate for further development for prevention and treatment of hepatic injury.
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Affiliation(s)
- Hai-Dan Yuan
- College of Pharmacy, Yan-Bian University, Yanji, Jilin Province 133000, China
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Yuan HD, Jin GZ, Piao GC. Protective Effects of the Supernatant of Ethanol Eluate from Artemisia sacrorum Ledeb. against Acetaminophen-Induced Liver Injury in Mice [corrected]. Biol Pharm Bull 2010; 32:1683-8. [PMID: 19801828 DOI: 10.1248/bpb.32.1683] [Citation(s) in RCA: 9] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
This study was designed to investigate the protective effects of the active part of Artemisia sacrorum Ledeb. Extract (ASE) against acetaminophen (APAP)-induced hepatotoxicity in mice. As a result, pretreated with ASE prior to the administration of APAP significantly prevented the increases of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and tumor necrosis factor-alpha (TNF-alpha) levels in serum, and glutathione (GSH) depletion, malondialdehyde (MDA) accumulation in liver tissue. In addition, ASE prevented APAP-induced apoptosis and necrosis, as indicated by a liver histopathological analysis and DNA laddering. Furthermore, according to the results from Western blot analysis, ASE markedly decreased APAP-induced caspase-3 and -8 protein expressions in mouse livers. All these results suggest that the protective effects of ASE against APAP-induced liver injury may involve mechanisms associated with its inhibitive effects of lipid peroxidation and the down-regulation of TNF-alpha mediated apoptosis.
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Affiliation(s)
- Hai-Dan Yuan
- College of Pharmacy, Yan-Bian University, Yanji, Jilin Province 133000, China
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