Role of genetic detection in peritoneal washes with gastric carcinoma: The past, present and future

Table 1 Primer sequences used for reverse transcriptase polymerase chain reaction

Gene	Sequence of primer pair (5’→3’)	Tm (°C)	Amplicon length (bp)
CEA	F; TCTGGAACTTCTCCTGGTCTCTCAGCTGG	69	160
	R; TGTAGCTGTTGCAAATGCTTTAAGGAAGAAGC
CK-20	F; GGTCGCGACTACAGTGCATATTACA	72	121
	R; CCTCAGCAGCCAGTTTAGCATTATC
Tripsinogen	F; ACCACCATGAATCCACTCCTG	62
	R; GCTTTAGCTATTGGCAGCTAT
MMP-7	F; ATGTTAAACTCCCGCGTCATA	72	418
	R; CAGCATACAGGAAGTTAATCC
DDC	F; AAGCACAGCCATCAGGATTCA	70
	R; TGGACATGCTTGCGGATATAAG
L3PP	F; GATGCTGTGTGTTTTGAT GTTGAC	95
	R; CTTGACTTGTTGCCTGATCACATT
MAGE A1-6	F; CTGAAGGAGAAGATCTGCC	60	855
	R; CTCCAGGTAGTTTTCCTGCAC

Tm: Melting temperature; F: Forward; R: Reverse; CEA: Carcinoembryonic antigen; CK-20: Cytokeratin-20; MMP-7: Matrix metalloproteinase-7; DDC: Dopa decarboxylase; L3PP: L-3 phosphoserine phosphatase heparanase; MAGE: Melanoma associated gene.

Table 2 Reports on molecular marker with peritoneal washes in gastric cancer

Ref.	Year	Method	Molecular marker	Outcome
Nakanishi et al[17]	1997	RT-PCR	CEA
Fujimura et al[46]	1998	RT-PCR	Trypsinogen
Kodera et al[20]	2002	RT-PCR	CEA	Peritoneal recurrence, survival
Nakanishi et al[17]	2000	Q-RT-PCR	CEA	Peritoneal recurrence, survival
Yonemura et al[6]	2001	RT-PCR	MMP-7
Sugita et al[14]	2003	Q-RT-PCR	CEA and CK-20	Peritoneal recurrence, survival
Sakakura et al[62]	2002	Q-RT-PCR	Dopa decarboylase
Shimomura et al[66]	2004	Q-RT-PCR	L3-PP
Kodera et al[7]	2006	RT-PCR	CEA	Overall survival
Jeon et al[13]	2010	RT-PCR	MAGE	Overall survival
Jeon et al[71]	2014	RT-PCR	MAGE and CEA	3 -yr survival

CEA: Carcinoembryonic antigen; CK-20: Cytokeratin-20; MMP-7: Matrix metalloproteinase-7; L3-PP: L-3 phosphoserine phosphatase heparanase; MAGE: Melanoma associated gene.