Aberrant crypt focus and fragile histidine triad protein in sporadic colorectal carcinoma

Table 1 Percentage of Fhit protein expression in hyperplastic aberrant crypt focus, dysplastic aberrant crypt focus and carcinoma n (%)

Fhit intensity	HACF	DACF	Carcinoma
No staining	0	0	12 (16)
Weak	18 (24)	9 (37.5)	18 (24)
Moderate	27 (36)	9 (37.5)	18 (24)
Strong	30 (40)	6 (25)	27 (36)
Total cases	75 (100)	24 (100)	75 (100)

HACF: Hyperplastic aberrant crypt focus; DACF: Dysplastic aberrant crypt focus; Fhit: Fragile histidine triad.

Table 2 Comparison of Fhit intensity between hyperplastic aberrant crypt focus, dysplastic aberrant crypt focus and carcinoma

Paired difference	mean	SD	t differentiation	P value
HACF vs DACF	0.25	0.46	1.528	0.17
HACF vs carcinoma	0.0952	0.77	0.568	0.576

HACF: Hyperplastic aberrant crypt focus; DACF: Dysplastic aberrant crypt focus.

Table 3 Fhit in hyperplastic aberrant crypt focus, dysplastic aberrant crypt focus and carcinoma vs sex

Fhit intensity	Hyperplastic ACF (P = 0.521)			Dysplastic ACF (P = 0.915)			Carcinoma (P = 0.269)
	Male	Female	Total	Male	Female	Total	Male	Female	Total
Weak	12	6	18	6	3	9	18		18
Moderate	24	3	27	6	3	9	15	3	18
Strong	21	9	30	3	3	6	18	9	27
Total	57	18	75	15	9	24	51	12	63

Fhit: Fragile histidine triad; ACF: Aberrant crypt focus.

Table 4 Fhit in hyperplastic aberrant crypt focus, dysplastic aberrant crypt focus and carcinoma vs age

Fhit intensity	HACF (P = 0.265)			DACF (P = 0.655)			Carcinoma (P = 0.036)
	≤50 yr	> 50 yr	Total	≤50 yr	> 50 yr	Total	≤50 yr	> 50 yr	Total
Weak	9	9	18		9	9	6	12	18
Moderate	6	21	27	6	3	9	9	9	18
Strong	6	24	30		6	6		27	27
Total	21	54	75	6	18	24	15	48	63

Fhit: Fragile histidine triad; HACF: Hyperplastic aberrant crypt focus; DACF: Dysplastic aberrant crypt focus.

Table 5 Fhit in hyperplastic aberrant crypt focus, dysplastic aberrant crypt focus and carcinoma vs vegetarian and non-vegetarian

Fhit intensity	HACF (P = 0.035)			DACF (P = 0.076)			Carcinoma (P = 0.037)
	Veg	Non Veg	Total	Veg	Non Veg	Total	Veg	Non Veg	Total
Weak	3	15	18	3	6	9		18	18
Moderate		27	27		9	9		18	18
Strong	15	15	30	6		6	12	15	27
Total	18	57	75	9	15	24	12	51	63

Fhit: Fragile histidine triad; Veg: Vegetarian; HACF: Hyperplastic aberrant crypt focus; DACF: Dysplastic aberrant crypt focus.

Table 6 Fhit intensity in hyperplastic aberrant crypt focus, dysplastic aberrant crypt focus and carcinoma vs smoking

Fhit intensity	HACF (P = 0.007)			DACF (P = 0.641)			Carcinoma (P = 0.213)
	Non Smoker	Smoker	Total	Non Smoker	Smoker	Total	Non Smoker	Smoker	Total
Weak	9	9	18	6	3	9	12	6	18
Moderate	9	18	27	6	3	9	6	12	18
Strong	30		30	6		6	21	6	27
Total	48	27	75	18	6	24	39	24	63

Fhit: Fragile histidine triad; HACF: Hyperplastic aberrant crypt focus; DACF: Dysplastic aberrant crypt focus.

Table 7 Fhit intensity in hyperplastic aberrant crypt focus, dysplastic aberrant crypt focus and carcinoma vs tumor differentiation

Tumor grade	Hyperplastic ACF (P = 0.719)				Dysplastic ACF (P = 0.232)				Carcinoma (P = 0.444)
Fhit→	+	++	+++	Total	+	++	+++	Total	-ive	+	++	+++	Total
Well diff		3		3	3			3	7	3			10
Mod diff	15	18	27	60	6	9	6	21	3	9	15	27	54
Poorly diff	3	6	3	12					2	6	3		11
Total	18	27	30	75	9	9	6	24	12	18	18	27	75

Fragile histidine triad (Fhit) protein grading: + = weak; ++ = moderate; +++ = strong; diff = differentiation; mod = moderate. ACF: Aberrant crypt focus.

Table 8 Fhit intensity in aberrant crypt focus vs tumor-node-metastasis stage

Fhit intensity	T (P = 0.325)					N (P = 0.938)			M (P = 0.396)
	Tis	T1	T2	T3	T4	N0	N1	N2	M0	M1	Total
Negative				9	3	12			12		12
Weak			3	14	1	10	8		18		18
Moderate				16	2	8	7	3	15	3	18
Strong				24	3	27	0		27		27
Total			3	63	9	57	15	3	72	3	75

Fhit: Fragile histidine triad; T: Tumor size; N: Lymph nodes; M: Metastasis.

Table 9 Ki67 expression pattern and Fhit intensity inhyperplastic aberrant crypt focus, dysplastic aberrant crypt focus

Ki67	HACF (P = 0.617)				DACF (P = 0.243)
Fhit intensity→	+	++	+++	Total	+	++	+++	Total
Basal			8	8
Expanded basal	5	9	13	27
Neck	13	18	9	40	3	2	5	10
Surface					6	7	1	14
Total	18	27	30	75	9	9	6	24

Fragile histidine triad (Fhit) intensity: + = weak; ++ = mod (moderate); +++ = strong. HACF: Hyperplastic aberrant crypt focus; DACF: Dysplastic aberrant crypt focus.