Application of single-cell sequencing in the study of immune cell infiltration in inflammatory bowel disease and colorectal cancer

Table 1 Comparison of performance and application of common single-cell sequencing platforms

Platform	Technical features	Data quality	Application scenarios	Comparative advantage
10× genomics	A system based on liquid droplets	High	High-throughput sequencing of cells	High-throughput, easy to operate, and large-scale cell atlas construction
BD rhapsody	A technology based on micropores	Medium	High unique molecular identifier detection sensitivity	Using imaging systems, populations with lower cell viability can be detected
Fluidigm C1	Isolation of single cells in a size and shape-dependent manner based on an automated microfluidic platform	High	High gene detection rate (> 10000)	Single-cell full-length transcripts were analyzed and complete cDNA libraries were prepared
ICELL8	Nanopore-based cell trapping and relying on cell and transcript specific barcodes to obtain individual cell transcript counts	High	High-throughput single-cell RNA sequencing	Ease of use and fast turnaround time allow experiments on a variety of biological samples
Illumina	Sequencing was performed based on the captured mtDNA	Medium	High-throughput sequencing of single cells	High throughput and high precision

cDNA: Complementary DNA; mtDNA: Mitochondrial DNA.