Tumor-derived exosomal miR-425-5p and miR-135b-3p enhance colorectal cancer progression through immune suppression and vascular permeability promotion

doi:10.4251/wjgo.v17.i6.106161

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastrointest Oncol. Jun 15, 2025; 17(6): 106161
Published online Jun 15, 2025. doi: 10.4251/wjgo.v17.i6.106161

Table 1 Primer sequence for real-time quantitative polymerase chain reaction

Gene	Primer sequence (5’-3’)
U6	Forward: CTCGCTTCGGCAGCACA
U6	Reverse: AACGCTTCACGAATTTGCGT
MiR-425-5p	Forward: AAUGACACGAUCACUCCCGUUGA
MiR-425-5p	Reverse: AGTGCAGGGTCCGAGGTATT
MiR-135b-3p	Forward: AUGUAGGGCUAAAAGCCAUGGG
MiR-135b-3p	Reverse: AGTGCAGGGTCCGAGGTATT
GAPDH	Forward: AATGACCCCTTCATTGAC
GAPDH	Reverse: TCCACGACGTACTCAGCGC
IL-1β	Forward: AACCTCTTCGAGGCACAAGG
IL-1β	Reverse: AGATTCGTAGCTGGATGCCG
IL-6	Forward: CCTTCGGTCCAGTTGCCTTCT
IL-6	Reverse: TCTGAGGTGCCCATGCTACA
TNF-α	Forward: CTGAGACTCTCCTGTGCAGC
TNF-α	Reverse: ACTCCCTGAAGAGACGGTGA
IL-4	Forward: CTTTGCTGCCTCCAAGAACA
IL-4	Reverse: GTTCCTGTCGAGCCGTTTCA

IL-1β: Interleukin 1 beta; IL-6: Interleukin 6; TNF-α: Tumor necrotic factor alpha; IL-4: Interleukin 4.

Table 2 Antibodies used in flow cytometry

Antibody	Catalog number	Manufacture
THP-1 cells
Anti-CD86-FITC	557343	BD Biosciences, NJ, United States
Anti-iNOS	ab283655	Abcam, United Kingdom
Alexa Fluor^® 488	ab236553	Abcam, United Kingdom
Anti-CD163-PE	560933	BD Biosciences, NJ, United States
Anti-CD206-APC	561763	BD Biosciences, NJ, United States
CD4⁺ T cells
Anti-CD4-FITC	561005	BD Biosciences, NJ, United States
Anti-IFN-γ-PE	561056	BD Biosciences, NJ, United States
Anti-IL4-PE	554485	BD Biosciences, NJ, United States
Anti-IL17A-PE	560438	BD Biosciences, NJ, United States
Anti-CD25-FITC	560990	BD Biosciences, NJ, United States
Anti-Foxp3-PE	560082	BD Biosciences, NJ, United States
Mouse tumor cells
Anti-CD4-FITC	553046	BD Biosciences, NJ, United States
Anti-IFNγ-PE	562020	BD Biosciences, NJ, United States
Anti-IL4-PE	562044	BD Biosciences, NJ, United States
Anti-IL17A-PE	561020	BD Biosciences, NJ, United States
Anti-CD25-APC	561048	BD Biosciences, NJ, United States
Anti-Foxp3-PE	563101	BD Biosciences, NJ, United States

CD86: T-lymphocyte activation antigen CD86; iNOS: Inducible nitric oxide synthase; CD163: Scavenger receptor cysteine-rich type 1 protein M130; CD206: Macrophage mannose receptor 1; CD4: T-cell surface glycoprotein CD4; IFN-γ: Interferon gamma; IL-4: Interleukin 4; CD25: Interleukin-2 receptor subunit alpha; FoxP3: Forkhead box protein P3.

Citation: Feng CZ, Zhong SQ, Ye SW, Zheng Z, Sun H, Zhou SH. Tumor-derived exosomal miR-425-5p and miR-135b-3p enhance colorectal cancer progression through immune suppression and vascular permeability promotion. World J Gastrointest Oncol 2025; 17(6): 106161
URL: https://www.wjgnet.com/1948-5204/full/v17/i6/106161.htm
DOI: https://dx.doi.org/10.4251/wjgo.v17.i6.106161