Clinical and Translational Research
Copyright ©The Author(s) 2024.
World J Gastrointest Oncol. Sep 15, 2024; 16(9): 3932-3954
Published online Sep 15, 2024. doi: 10.4251/wjgo.v16.i9.3932
Table 1 Primers used in real-time polymerase chain reaction
Gene
Direction
Sequence
IL-6FORWARDTGGTGTTGCCTGCTGCCTTC
REVERSEGCTGAGATGCCGTCGAGGATG
CCL2FORWARDATGAAAGTCTCTGCCGCCCTTC
REVERSEGACACTTGCTGCTGGTGATTCTTC
PTGS2FORWARDAAGTCCCTGAGCATCTACGGTTTG
REVERSETGTTCCCGCAGCCAGATTGTG
HMOX1FORWARDCTACCGCTCCCGCATGAACTC
REVERSEAGCAGGAACGCAGTCTTGGC
MMP9FORWARDTGGTCCTGGTGCTCCTGGTG
REVERSETGCCTGTCGGTGAGATTGGTTC
MMP2FORWARDCCTACACCAAGAACTTCCGTCTG
REVERSEGTGCCAAGGTCAATGTCAGGAG
Table 2 Binding energy results for molecular docking
Mol ID
Molecule name
Binding affinity (kcal/mol)
PTGS2
MMP9
MMP2
IL6
HMOX1
CCL2
MOL000098Quercetin-3.38-7.92-3.33-4.36-4.15-5.12
MOL004828Glepidotin A-3.67-4.72-3.88-5.52-4.18-5.24
MOL0049917-Acetoxy-2-methylisoflavone-5.52-7.89-4.66-5.59-5.54-6.06
MOL005000Gancaonin G-4.33-6.05-3.56-3.88-4.73-5.34
MOL005003Licoagrocarpin-4.54-7.22-3.98-5.72-5.56-6.65
MOL005020Dehydroglyasperins C-3.62-6.12-3.27-3.76-4.69-4.97