Long noncoding RNA steroid receptor RNA activator 1 inhibits proliferation and glycolysis of esophageal squamous cell carcinoma

Table 1 Quantitative reverse transcription-polymerase chain reaction primer sequences

Name	Primer sequence
lncRNA SRA1	F: 5′-GCTGGGCACTGGGAATGTAA-3′
	R: 5′-CACGACCCTACAACCCTCTG-3′
miRNA-363-5p	F: 5′-GGCGAGTTTTAATTTCTATT-3′
	R: 5′-ATCAACTGCTCTCGTGGA-3′
LHPP	F: 5′-GACGCAGCACTCACCCATCT-3′
	R: 5′-CCAGGCATTCGGTGATGTG-3′
GAPDH	F: 5′-AGAAGGCTGGGGCTCATTTG-3′
	R: 5′-GCAGGAGGCATTGCTGATGAT-3′
U6	F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′
	R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′

LncRNA: Long non-coding RNA; SRA1: Steroid receptor RNA activator 1; LHPP: Phospholysine phosphohistidine inorganic pyrophosphate phosphatase.

Table 2 Correlations between steroid receptor RNA activator 1 expression and clinical characteristics in esophageal squamous cell carcinoma patients

Characteristics	Case (n = 38)	SRA1 expression		χ2	P value
		High	Low
Sex
Male	26	12	14	0.487	0.485
Female	12	7	5
Age (years)				1.310	0.252
≤ 60	9	3	6
> 60	29	16	13
Pathological differentiation grade				0.186	0.911
Well	7	4	3
Moderately	23	11	12
Poorly	8	4	4
T Stage				5.265	0.022a
T1-T2	20	13	7
T3-T4	18	5	13
TNM Stage				8.622	0.003b
I-II	17	13	4
III-IV	21	6	15
Lymph node metastasis				8.526	0.004b
Negative	19	14	5
Positive	19	5	14

^aP < 0.05.

^bP < 0.01.

The χ² test was used for comparison between groups. SRA1: Steroid receptor RNA activator 1.