Diagnostic accuracy of the multi-target stool DNA test in detecting colorectal cancer: A hospital-based study

Table 1 Basic demographic characteristics of cases and controls, n (%)

Variable	Case group		Control group
	Colorectal cancer	Adenomas	Healthy subjects
Gender
Female	36 (70.60)	15 (65.20)	31 (51.81)
Male	15 (29.49)	8 (34.80)	30 (49.18)
Age
mean ± SD	66.14 ± 9.47	60.13 ± 12.40	54.18 ± 10.30
< 60 yr	16 (31.40)	10 (43.50)	42 (68.90)
≥ 60 yr	35 (68.60)	13 (56.50)	19 (31.10)
Education level
Junior high school and below	42 (82.40)	14 (60.90)	16 (26.20)
Senior high school and above	9 (17.60)	9 (39.10)	45 (73.80)
BMI
< 23.00	31 (60.80)	12 (52.20)	21 (34.42)
≥ 23.00	20 (39.20)	11 (47.80)	40 (65.57)
Tumor location
Colon	24 (47.06)	-	-
Rectum	27 (52.94)	-	-
Pathogenic type		-	-
Protruding type	7 (13.73)	-	-
Infiltrating type	4 (7.84)	-	-
Ulcerative type	40 (78.43)	-	-
Differentiation		-	-
High	4 (7.84)	-	-
Medium	37 (72.55)	-	-
Low	10 (19.61)	-	-
Histological type		-	-
Adenocarcinoma	49 (95.74)	-	-
Other types	2 (4.26)	-	-
Dukes stage		-	-
A	40 (78.43)	-	-
B	10 (19.61)	-	-
C	1 (1.06)	-	-
D	0 (0.0)	-	-

BMI: Body mass index.

Table 2 Evaluation of the expression of different tumor markers between cases and controls, n (%)

Variable	Case group		Control group	P value
	Colorectal cancer	Adenomas	Healthy subjects	CRC patients vs healthy controls	Adenoma patients vs healthy controls
AFP
mean ± SD	5.87 ± 17.59	3.03 ± 1.57	3.32 ± 1.36	0.03	0.43
≤ 7 µg/L	47 (92.20)	22 (95.70)	61 (100.00)
> 7.1 µg/L	4 (7.80)	1 (4.30)	0 (0.00)
CEA
mean ± SD	37.12 ± 149.74	5.21 ± 3.58	2.20 ± 1.58	0.00	0.00
≤ 5 µg/L	34 (66.70)	11 (47.80)	56 (91.80)
> 5.1 µg/L	17 (33.30)	12 (52.20)	5 (8.20)
CA199
mean ± SD	63.05 ± 276.78	13.39 ± 10.19	9.77±8.89	0.01	0.15
≤ 25 µ/mL	41 (80.40)	18 (78.30)	59 (96.70)
> 25.1 µ/mL	10 (19.60)	5 (21.70)	2 (3.30)
Complex value
mean ± SD	806.54 ± 289.28	351.61 ± 369.85	105.11 ± 90.95	0.00	0.00
< 165	5 (9.80)	13 (56.52)	59 (96.72)
≥ 165	46 (90.20)	10 (43.48)	2 (3.28)

AFP: Alpha-feto protein; CEA: Carcinoembryonic antigen; CA199: Carbohydrate antigen 199.

Table 3 Diagnostic value of tumor markers and multi-target stool DNA test in colorectal cancer

Detection method	AUC (%)	Sensitivity (%)	Specificity (%)	P value
AFP	41.3	35.3	48.8	0.264
CEA	73.2	60.8	63.1	0.001
CA199	62.5	49.0	69.0	0.069
DNA	93.3	90.2	83.3	0.000
DNA+CEA	94.7	90.2	75.0	0.000

AFP: Alpha-feto protein; CEA: Carcinoembryonic antigen; CA199: Carbohydrate antigen 199.

Table 4 Diagnostic value of tumor markers and multi-target stool DNA test in adenoma

Detection method	AUC (%)	Sensitivity (%)	Specificity (%)	P value
AFP	42.1	34.8	54.1	0.263
CEA	76.1	69.6	62.3	0.000
CA199	59.2	56.5	65.6	0.196
DNA	73.1	56.5	68.9	0.001
DNA+CEA	80.4	78.3	60.7	0.000

AFP: Alpha-feto protein; CEA: Carcinoembryonic antigen; CA199: Carbohydrate antigen 199.