Role of Raman spectroscopy and surface enhanced Raman spectroscopy in colorectal cancer

Figure 1 Energy shifts involved in light scattering interactions. Adapted from Lin et al[23].

Figure 2 Example Raman spectrum of dried human serum. Spectrum of dried droplet of human serum taken with 785 nm laser excitation, 10 s acquisition time with an InVia Raman spectrometer (Renishaw, United Kingdom).

Figure 3 An example spectrum of a surface enhanced Raman spectroscopy response vs Raman response of human blood plasma. Raman spectrum and SERS spectrum of dried human plasma droplet at 10% laser power with 785 nm laser excitation, 10 s acquisition time with an InVia Raman spectrometer (Renishaw, United Kingdom). SERS response gained by 1:1 mixing with 40 nm raw gold nanoparticles (Nanocs, United States). Purple: SERS response of plasma; Blue: Raman response of plasma. SERS: Surface enhanced Raman spectroscopy.

Figure 4 Different methods of producing a surface enhanced Raman spectroscopy response with biological samples. A: Mixing based methods with both non-labeled mixing and labeled or “aggregation” based mixing; B: Labeled surface enhanced Raman spectroscopy based immunoassay. SERS: Surface enhanced Raman spectroscopy.