Original Article
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastrointest Oncol. Aug 15, 2014; 6(8): 289-300
Published online Aug 15, 2014. doi: 10.4251/wjgo.v6.i8.289
Figure 1
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Figure 1 Effects of polyphenols on cell growth. A: Growth of HCT8-â8-expressing cells in the presence of genistein and 17â-E2; B: Growth of HCT8-β8-expressing cells in the presence of quercetin and 17β-E2; C: Growth of HCT8-pSV2neo-expressing cells in the presence of genistein and 17β-E2; D: Growth of HCT8-pSV2neo-expressing cells in the presence of quercetin and 17β-E2. Values are the means of triplicates; aP < 0.05 vs control; bP < 0.01 vs control.
Figure 2
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Figure 2 Treatment of HCT8-β8-expressing cells with genistein. HCT8-β8-expressing cells were treated with various concentrations of genistein for 48 h (A-F) or 144 h (G-L) and stained with acridine orange. Nuclei and mitochondria appear green, whereas lysosomes appear red-orange under fluorescence, adjacent to corresponding phase contrast images (magnification × 20).
Figure 3
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Figure 3 Treatment of HCT8-β8-expressing cells with quercetin. HCT8- β8-expressing cells were treated with various concentrations of quercetin for 48 h (A-F) or 144 h (G-L) and stained with acridine orange. Nuclei and mitochondria appear green, whereas lysosomes appear red-orange under fluorescence, adjacent to corresponding phase contrast images (magnification, × 20).
Figure 4
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Figure 4 Treatment of cells with 17β-E2. A-D: HCT8-β8-expressing cells; or E-H: HCT8-pSV2neo-expressing cells were treated with 10 nmol/L 17β-E2 for 48 h (A, B, E, F) or 144 h (C, D, G, H) and stained with acridine orange. Nuclei and mitochondria appear green, whereas lysosomes appear red-orange under fluorescence, adjacent to corresponding phase contrast images (magnification × 20).
Figure 5
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Figure 5 Treatment of HCT8-pSV2neo-expressing cells with genistein. A-F: HCT8-pSV2neo-expressing cells were treated with 25 μmol/L (B and E) or 100 μmol/L (C and F) genistein for 48 h (A-C) or 144 h (D-F) and stained with acridine orange. Nuclei and mitochondria appear green, whereas lysosomes appear red-orange under fluorescence, adjacent to corresponding phase contrast images (magnification × 20).
Figure 6
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Figure 6 Treatment of HCT8-pSV2neo-expressing cells with quercetin. A-F: HCT8-pSV2neo-expressing cells were treated with 25 μmol/L (B and E) or 100 μmol/L (C and F) quercetin for 48 h (A-C) or 144 h (D-F) and stained with acridine orange. Nuclei and mitochondria appear green, whereas lysosomes appear red-orange under fluorescence, adjacent to corresponding phase contrast images (magnification × 20).
Figure 7
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Figure 7 Induction of EREtkLUC reporter gene activity. Treatment of HCT8-â8-expressing cells with 17â-E2, genistein and quercetin induces EREtk expression observed as relative luciferase activity. Values are the mean ± SD of triplicates; aP < 0.05 vs control.
Figure 8
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Figure 8 Expression of ERβ mRNA levels by quantitative real-time reverse transcription-polymerase chain reaction. Induction of ERβ expression by 17β-E2, genistein and quercetin in A: HCT8-β8-expressing cells; B: HCT8-pSV2neo-expressing cells. The results are expressed relative to RPS18 mRNA levels. Values are the mean ± SD of quadruplicates; aP < 0.05 vs control.