Brief Article
Copyright ©2011 Baishideng Publishing Group Co.
World J Gastrointest Oncol. Feb 15, 2011; 3(2): 24-32
Published online Feb 15, 2011. doi: 10.4251/wjgo.v3.i2.24
Figure 1
Figure 1 Gel electrophoresis of PCR products from case 1, obtained with 6 primer sets amplifying the FR1-JH region (A) and the FR3-JH region (B). Experiments were performed in duplicate. For all amplifications, the reverse primer was JH. A: The forward primers used are indicated on the gel. The agarose gel shows predominant bands with primer sets VH3-JH and VH5-JH; B: Polyacrylamide gel showing FR3-JH amplicons of the same length starting from the VH3-JH PCR product (lanes 1, 2) and from the initial DNA sample (lanes 5, 6). No monoclonal FR3-JH product was obtained starting from the VH5-JH PCR product (lanes 3, 4). These results indicate that the lymphoma cells use a VH3 family gene and that the product obtained with the VH5-JH set is an unspecific product.
Figure 2
Figure 2 Mutation analysis of the DP-49 gene used in cases 1 and 7, compared to the germline DP-49 gene sequence. CDR regions are indicated. Dashes indicate sequence similarity. Replacement mutations are shown in bold.