Association between Helicobacter pylori infection, mismatch repair, HER2 and tumor-infiltrating lymphocytes in gastric cancer

Figure 1 Pathological images of a case of gastric cancer with Helicobacterpylori infection with a low level of tumor-infiltrating lymphocytes. A: Hematoxylin and eosin (HE) staining showing the presence of Helicobacter pylori (yellow arrow) at 100 × magnification; B: HE staining of the intratumoral compartment with a low level of tumor-infiltrating lymphocytes at 20 ×.

Figure 2 Pathological images of a case of gastric cancer with deficient mismatch-repair and a high level of tumor-infiltrating lymphocytes. A: Hematoxylin and eosin (HE) staining of stromal compartment with high level of tumor-infiltrating lymphocytes (TILs) at 20 × magnification; B: Field magnification of HE with high TIL stain at 40 ×; C: CD3 immunohistochemistry (IHC) staining at 40 ×; D: Identification of CD3 density by machine learning-based image processing showing positive (green) and negative (blue) cells; E: CD8 IHC staining; F: Digital identification of CD8 density; G: IHC staining showing absence of MSH6 expression and; H: Absence of PMS2 expression.

Figure 3 Overall survival analyses. A: Kaplan-Meier overall survival curve according to clinical stage; B: Intratumoral CD3/163 ratio; C: Intratumoral CD3/CD8 ratio.