Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Sep 15, 2023; 15(9): 1531-1543
Published online Sep 15, 2023. doi: 10.4251/wjgo.v15.i9.1531
RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer
Xue-Jun Wang, Yong Liu, Bin Ke, Li Zhang, Han Liang
Xue-Jun Wang, Yong Liu, Bin Ke, Li Zhang, Han Liang, Department of Gastric Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Tianjin Key Laboratory of Digestive Cancer, Tianjin 300060, China
Author contributions: Wang XJ designed and wrote the manuscript; Liu Y, Ke B, Zhang L, and Liang H collected and analyzed the data; and all authors read and approved the final manuscript.
Supported by the National Key R&D Program of China, No. 2016YFC1303202.
Institutional review board statement: The study was approved by the Ethics Committee of Tianjin Medical University Cancer Institute and Hospital (Approval No. E2020216).
Informed consent statement: Written informed consent was obtained from all patients.
Institutional animal care and use committee statement: This work was reviewed and approved by the Animal Care and Use Committee of Beijing Viewsolid Biotechnology Co. LTD (Approval No. VS212601449).
Conflict-of-interest statement: The authors report no conflict of interest for this article.
Data sharing statement: The data that support the findings of this study are available from the corresponding author.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Xue-Jun Wang, MD, Professor, Department of Gastric Oncology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin’s Clinical Research Center for Cancer, Tianjin Key Laboratory of Digestive Cancer, Huanhu Xi Road, Tiyuan Bei, Hexi District, Tianjin 300060, China. wangxuejun20202020@163.com
Received: April 7, 2023
Peer-review started: April 7, 2023
First decision: July 19, 2023
Revised: July 31, 2023
Accepted: August 18, 2023
Article in press: August 18, 2023
Published online: September 15, 2023
ARTICLE HIGHLIGHTS
Research background

Previous studies have illustrated that integrin binding sialoprotein (IBSP) exhibits a promotive role in the progression of cancers. However, the regulatory functions of IBSP in gastric cancer (GC) progression remain unclear.

Research motivation

To find effective bio-targets for GC prognosis and treatment.

Research objectives

To probe the regulatory effects and underlying molecular mechanism of IBSP in GC progression.

Research methods

Real-time quantitative polymerase chain reaction and Western blot were used to detect the mRNA and protein expression of IBSP, respectively. The prognosis of GC patients with high or low IBSP expression was evaluated. The regulatory effects of IBSP in GC progression was assessed via in vitro and in vivo experiments. The molecular mechanism of the IBSP/cleavage and polyadenylation factor 6 (CPSF6) axis was validated.

Research results

IBSP exhibited higher expression in GC, and IBSP knockdown suppressed cell proliferation, migration, and invasion but facilitated pyroptosis. Moreover, the results revealed that CPSF6 binds to the 3’-untranslated region of IBSP and positively regulates IBSP expression in GC.

Research conclusions

Other regulatory functions and related mechanisms of ISBP in GC may be investigated in the future, and its application in GC treatment will be explored.

Research perspectives

IBSP expression is upregulated in GC tissues and cells, which results in a poor prognosis in GC. CPSF6 positively regulates IBSP to affect pyroptosis and aggravate tumor growth in GC.