• DNA methyltransferase 1
• Gastric cancer
• Methylation
• SERPINE1
• microRNA-125a-5p

• Competing endogenous RNA
• Cross-cancer analysis
• Digestive system tumor
• Prognosis

• ERBB2
• LIFR
• STAT3
• RPL
• gestation and abortion
• miR‐125a
• pregnancy

• Humans
• Female
• Abortion, Habitual/genetics
• MicroRNAs/genetics
• Pregnancy
• Adult
• STAT3 Transcription Factor/genetics
• STAT3 Transcription Factor/metabolism
• Leukemia Inhibitory Factor Receptor alpha Subunit/genetics
• Receptor, ErbB-2/genetics
• Case-Control Studies
• Young Adult

• SR/WOS-A/LS-406/2018 Department of Science and Technology, India

• HDAC2
• PHOX2B
• histone modification
• microRNA-125a
• neuroblastoma

• miR-125a-5p
• Hippo pathway
• TAZ
• gastric cancer
• prognosis

• Adenocarcinoma/genetics
• Adenocarcinoma/metabolism
• Adenocarcinoma/pathology
• Animals
• Cell Line, Tumor
• Epithelial-Mesenchymal Transition/genetics
• Gene Expression Regulation, Neoplastic
• Hippo Signaling Pathway/genetics
• Humans
• Male
• Mice, Nude
• MicroRNAs/genetics
• Receptor, EphA2/genetics
• Receptor, EphA2/metabolism
• Stomach Neoplasms/genetics
• Stomach Neoplasms/metabolism
• Stomach Neoplasms/pathology
• TEA Domain Transcription Factors/genetics
• TEA Domain Transcription Factors/metabolism
• Transcriptional Coactivator with PDZ-Binding Motif Proteins/genetics
• Transcriptional Coactivator with PDZ-Binding Motif Proteins/metabolism
• Xenograft Model Antitumor Assays
• Mice

• 202102310117 Key R & D and promotion projects of Henan Province in 2019
• 2015JJ4049 Natural Scientific Foundation of Hunan province
• 81172297 National Natural Scientific Foundation of China
• 81472692 National Natural Scientific Foundation of China
• 20B320029 Key scientific research projects of colleges and universities in Henan Province

Despite recent progress, gastric cancer remains one of the most common cancers and has a high mortality rate worldwide. Aberrant DNA methylation pattern and deregulation of noncoding RNA expression appear in the early stages of gastric cancer. Numerous investigations have confirmed their significant role in gastric cancer tumorigenesis and their high potential as diagnostic and prognostic biomarkers. Currently, it is clear that these epigenetic regulators do not work alone but interact with each other, generating a complex network. The aim of our review was to summarize the current knowledge of this interaction in gastric cancer and estimate its clinical potential for the diagnosis, prognosis, and treatment of the disease.

• DNA methylation
• biomarker
• epigenetics
• gastric cancer
• noncoding RNAs

• DNA Methylation
• DNA, Neoplasm/genetics
• DNA, Neoplasm/metabolism
• Epigenesis, Genetic
• Gene Expression Regulation, Neoplastic
• Humans
• RNA, Neoplasm/biosynthesis
• RNA, Neoplasm/genetics
• RNA, Untranslated/biosynthesis
• RNA, Untranslated/genetics
• Stomach Neoplasms/genetics
• Stomach Neoplasms/metabolism
• Stomach Neoplasms/pathology

Colorectal cancer is currently the third largest malignant tumor in the world, with high new cases and high mortality. Metastasis is one of the most common causes of death of colorectal cancer, of which liver metastasis is the most fatal. Since the beginning of the Human Genome Project in 2001, people have gradually recognized the 3 billion base pairs that make up the human genome, of which only about 1.5% of the nucleic acid sequences are used for protein coding, including proto-oncogenes and tumor suppressor genes. A large number of differences in the expression of proto-oncogenes and tumor suppressor genes have also been found in the study of colorectal cancer, which proves that they are also actively involved in the progression of colorectal cancer and promote the occurrence of liver metastasis. Except for 1.5% of the coding sequence, the rest of the nucleic acid sequence does not encode any protein, which is called non-coding RNA. With the deepening of research, genome sequences without protein coding potential that were originally considered “junk sequences” may have important biological functions. Many years of studies have found that a large number of abnormal expression of ncRNA in colorectal cancer liver metastasis, indicating that ncRNA plays an important role in it. To explore the role and mechanism of these coding sequences and non-coding RNA in liver metastasis of colorectal cancer is very important for the early diagnosis and treatment of liver metastasis of colorectal cancer. This article reviews the coding genes and ncRNA that have been found in the study of liver metastasis of colorectal cancer in recent years, as well as the mechanisms that have been identified or are still under study, as well as the clinical treatment of liver metastasis of colorectal cancer.

• colorectal cancer
• liver metastasis
• mechanisms
• treatment

The carcinogenesis and progression of cervical cancer is a complex process in which numerous microRNAs are involved. The purpose of this study is to investigate the role of miR-125a-5p in progression of cervical cancer.

RT-qPCR was used to detect the expression of miR-125a-5p and GALNT7 in cervical cancer tissues and cell lines. Then, the miR-125a-5p mimic, miR-125a-5p inhibitor, GALNT7 siRNA, or/and pcDNA-GALNT7 were respectively transfected into HeLa and Caski cervical cancer cells, and Cell Counting kit-8 assay, Transwell assay and flow cytometry analysis were respectively used to observe cell proliferation, invasion and apoptosis. Subsequently, luciferase reporter gene assay was employed in confirming the target relationship between miR-125a-5p and GALNT7. MiR-125a-5p mimic or/and pcDNA-GALNT7 were transfected into the cervical cancer cells at the absence of epidermal growth factor (EGF) or not, and the pcDNA-GALNT7 was transfected into the cervical cancer cells at the absence of inhibitors of multiple kinases or not. Furthermore, the effect of miR-125a-5p on tumor growth was also studied using a xenograft model of nude mice.

MiR-125a-5p was down-regulated in both cervical cancer tissues and cell lines and it inhibited cell proliferation and invasion of cervical cancer cells. MiR-125a-5p directly targeted and post-transcriptionally downregulated GALNT7 that was strongly upregulated in cervical cancer tissues and cell lines. Similar to the effect of miR-125a-5p mimic, silencing GALNT7 inhibited proliferation and invasion of cervical cancer cells. In addition, miR-125a-5p overexpression could counteract both GALNT7- and EGF-induced cell proliferation and invasion. GALNT7 promoted cell proliferation and invasion by activating the EGFR/PI3K/AKT kinase pathway, which could be abated by the inhibitors of the kinases. Moreover, the role of miR-125a-5p inhibited tumor formation in cervical cancer by suppressing the expression of GALNT7 in vivo.

In conclusion, miR-125a-5p suppressed cervical cancer progression by post-transcriptionally downregulating GALNT7 and inactivating the EGFR/PI3K/AKT pathway.

• Cervical cancer
• GALNT7
• MiR-125a-5p
• The EGFR/PI3K/AKT pathway

• cancer
• clinicopathologic parameter
• meta-analysis
• microrna-125a-5p
• prognosis

• Biomarkers, Tumor/blood
• Gene Expression Regulation, Neoplastic
• Genes, Tumor Suppressor
• Humans
• Lymphatic Metastasis
• MicroRNAs/blood
• Neoplasms/blood
• Observational Studies as Topic
• Proportional Hazards Models

microRNAs (miRs) serve critical roles in tumor progression. Low expression of miR-125a in gastric carcinoma (GC) may promote tumor development. In the present study, low expression of miR-125a was confirmed in cancer tissues using The Cancer Genome Atlas database. Additionally, the expression and clinical significance of miR-125a-5p was investigated using reverse transcription-quantitative PCR in 150 cases of GC. The results of the present study demonstrated that the level of miR-125a-5p expression was decreased in GC biopsies compared with that in matched adjacent normal tissues. Low expression of miR-125a-5p was associated with increased tumor diameter, high Ki67 expression and poor overall survival of patients with GC. Multivariate survival analysis demonstrated that low miR-125a-5p expression may be used as an independent prognostic factor for patients with GC. However, no effects on the cell viability in a Cell Counting kit-8 assay, and cell migration and invasion in Transwell assays were detected in response to treatment using miR-125a-5p mimics or inhibitors in vitro. Therefore, the results of the present study provide evidence that low expression of miR-125a-5p may be associated with a poor prognosis, suggesting its value as a tumor biomarker for patients with GC.

• The Cancer Genome Atlas
• gastric cancer
• microRNA-125a
• survival

Objective: Many studies have shown that long non-coding RNAs (lncRNAs) are closely related to various cancers. This study aims to explore the roles of lncRNA HOXA11-AS in the development and progression of osteosarcoma (OS).

Methods: The expression levels of HOXA11-AS and miR-125a-5p in tumor tissues and the adjacent tissues were detected by RT-PCR method. The proliferation, migration and invasion of MG-63 and KHOS cells were determined.

Results: It was found that HOXA11-AS expression levels in OS tissues and OS cell lines were higher than those in OS adjacent tissues and normal human osteoblast cell lines. The higher expression level of HOXA11-AS was positively correlated with more severe clinical stage, distant metastasis and poor prognosis of OS. Inhibition of HOXA11-AS expression could reduce metastasis and invasion of OS cell lines. In addition, HOXA11-AS was found to be an endogenous inhibitor of miR-125a-5p, it down regulated the expression level of miR-125a-5p, and this process could promote the expression of Rab3D, the target gene of miR-125a-5p.

Conclusion: Our study elucidated the role of a new HOXA11-AS/miR-125a-5p/Rab3D regulatory pathway in promoting OS metastasis.

• HOXA11-AS
• Rab3D
• long non-coding RNAs (lncRNAs)
• miR-125a-5p
• osteosarcoma (OS)

• Biomarker
• Cancer
• Epigenetic
• Immune cell
• MicroRNA

• HAS1
• STAT3
• gastric cancer
• miR-125a

• Cancer
• RNA interference
• cell cultures
• hepatocellular carcinoma
• microRNA
• oncosuppressor.

• cancer stem-like cells
• cell cycle arrest
• miR-125a
• nasopharyngeal carcinoma
• p53

• Suv39H1
• miR-125a-5p
• non-small-cell lung cancer

• A549 Cells
• Animals
• Biomarkers, Tumor/genetics
• Biomarkers, Tumor/metabolism
• Carcinoma, Non-Small-Cell Lung/metabolism
• Carcinoma, Non-Small-Cell Lung/pathology
• Cell Movement
• Cell Proliferation
• CpG Islands
• Disease Progression
• Humans
• Lung Neoplasms/metabolism
• Lung Neoplasms/pathology
• Methylation
• Methyltransferases/genetics
• Methyltransferases/metabolism
• Mice
• Mice, Nude
• MicroRNAs/genetics
• MicroRNAs/metabolism
• Middle Aged
• Repressor Proteins/genetics
• Repressor Proteins/metabolism
• Xenograft Model Antitumor Assays

• Liaoning Province Science Research Career Funds for Public Welfare

Emerging evidence suggests that microRNAs (miRNAs) serve an important role in tumorigenesis and development. Although the low expression of miR‐125a‐5p in gastric cancer has been reported, the underlying mechanism remains unknown. In the current study, the low expression of miR‐125a‐5p in gastric cancer was verified in paired cancer tissues and adjacent non‐tumour tissues. Furthermore, the GC islands in the miR‐125a‐5p region were hypermethylated in the tumour tissues. And the hypermethylation was negatively correlated with the miR‐125a‐5p expression. Target gene screening showed that the histone methyltransferase Suv39H1 was one of the potential target genes. In vitro studies showed that miR‐125a‐5p could directly suppress the Suv39H1 expression and decrease the H3K9me3 levels. On the other hand, the Suv39H1 could induce demethylation of miR‐125a‐5p, resulting in re‐activation of miR‐125a‐5p. What is more, overexpessing miR‐125a‐5p could also self‐activate the silenced miR‐125a‐5p in gastric cancer cells, which suppressed cell migration, invasion and proliferation in vitro and inhibited cancer progression in vivo. Thus, we uncovered here that the epigenetic silenced miR‐125a‐5p could be self‐activated through targeting Suv39H1 in gastric cancer, suggesting that miR‐125a‐5p might be not only the potential prognostic value as a tumour biomarker but also potential therapeutic targets in gastric cancer.

• Suv39H1
• epigenetic silence
• gastric cancer
• miR-125a-5p

• EBV infection
• gastric cancer
• gene expression profile
• gene mutation
• miRNA
• microsatellite
• molecular gastric cancer subtype
• preclinical models

• Animals
• Epigenesis, Genetic
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs/genetics
• MicroRNAs/metabolism
• Microsatellite Instability
• Mutation
• Signal Transduction
• Stomach Neoplasms/classification
• Stomach Neoplasms/genetics
• Stomach Neoplasms/metabolism
• Stomach Neoplasms/pathology
• Transcriptome

• Cancer
• Cell proliferation
• Hepatocarcinoma
• RNA interference
• microRNA

Human microRNA-125a-5p (miR-125a) is expressed in most tissues where it downregulates the expression of membrane receptors or intracellular transductors of mitogenic signals, thus limiting cell proliferation. Expression of this miRNA generally increases with cell differentiation whereas it is downregulated in several types of tumors, such as breast, lung, ovarian, gastric, colon, and cervical cancers, neuroblastoma, medulloblastoma, glioblastoma, and retinoblastoma. In this study, we focused on hepatocellular carcinoma and used real-time quantitative PCR to measure miR-125a expression in 55 tumor biopsies and in matched adjacent non-tumor liver tissues. This analysis showed a downregulation of miR-125a in 80 % of patients, with a mean decrease of 4.7-fold. Comparison of miRNA downregulation with clinicopathological parameters of patients didn't yield significant correlations except for serum bilirubin. We then evaluated the expression of known targets of miR-125a and found that sirtuin-7, matrix metalloproteinase-11, and c-Raf were up-regulated in tumor tissue by 2.2-, 3-, and 1.7-fold, respectively. Overall, these data support a tumor suppressor role for miR-125a and encourage further studies aimed at the comprehension of the molecular mechanisms governing its expression, eventually leading to treatments to restore its expression in tumor cells.

• cell proliferation
• gene regulation
• hepatocellular carcinoma
• microRNA
• tumor

miR-125a usually functions as a tumor suppressor in cancers. However, the role of miR-125a in laryngeal squamous cell carcinoma (LSCC) has not been determined.

qRT-PCR was applied to measure the expression of miR-125a and HK2 mRNA in LSCC tissues and cells. CCK-8 kit and flow cytometry analysis were performed to detect cell viability and apoptosis. Luciferase reporter assay and RNA immunoprecipitation (RIP) were conducted to confirm the relationship between miR-125a and HK2. Commercial test kits were used to determine the concentrations of glucose and l-lactate. Xenograft in mice was constructed to validate the function and mechanism of miR-125a in LSCC tumor growth.

A negative correlation was found between miR-125a expression and the level of Hexokinase 2 (HK2) mRNA in LSCC tissues. Functional experiments found that miR-125a inhibited viability and glycolysis and induced apoptosis in LSCC cells. Similarly, HK2 downregulation led to viability and glycolysis inhibition and induction of apoptosis in LSCC cells in vitro. Moreover, miR-125a overexpression suppressed LSCC xenograft growth in vivo. Mechanically, HK2 was verified to be a target of miR-125a by luciferase reporter assays and RNA immunoprecipitation (RIP) assays. Furthermore, restored HK2 expression reversed miR-125a-mediated proliferation and glycolysis inhibition and induction of apoptosis in LSCC cells.

miR-125a suppressed LSCC progression by targeting HK2 in vitro and in vivo, suggesting that miR-125a may be a potential molecular target for LSCC treatment.

The online version of this article (doi:10.1186/s13578-017-0178-y) contains supplementary material, which is available to authorized users.

• Apoptosis
• Glycolysis
• HK2
• Proliferation
• miR-125a

• HOXA11 antisense RNA (HOXA11-AS)
• colorectal cancer (CRC)
• liver metastasis
• long noncoding RNA (lncRNA)
• peptidyl arginine deiminase 2 (PADI2)

MicroRNA-125a-5p (miR-125a) is a vertebrate homolog of lin-4, the first discovered microRNA, and plays a fundamental role in embryo development by downregulating Lin-28 protein. MiR-125a is also expressed in differentiated cells where it generally acts as an antiproliferative factor by targeting membrane receptors or intracellular transductors of mitogenic signals. MiR-125a expression is downregulated in several tumors, including hepatocellular carcinoma (HCC) where it targets sirtuin-7, matrix metalloproteinase-11, VEGF-A, Zbtb7a, and c-Raf. In this study, we have isolated the transcription promoter of human miR-125a and characterized its activity in HCC cells. It is a TATA-less Pol II promoter provided with an initiator element and a downstream promoter element, located 3939 bp upstream the genomic sequence of the miRNA. The activity of the promoter is increased by the transcription factor NF-kB, a master regulator of inflammatory response, and miR-125a itself was found to strengthen this activation through inhibition of TNFAIP3, a negative regulator of NF-kB. This finding contributes to explain the increased levels of miR-125a observed in the liver of patients with chronic hepatitis B.

• bevacizumab
• metastatic colorectal cancer
• microRNA
• predictive biomarker
• progression-free survival

Decline of transcription factor FoxN1, which predominantly regulates thymic epithelial cell (TEC) differentiation and homeostasis lifelong, is demonstrated to be casually related to age-related thymic involution. Whereas, a global role of microRNAs (miRNAs) has also been demonstrated to control and maintain TEC-constituting thymic microenvironment and to be changed in expression profile in the aged thymus. Therefore, it is urgently necessary to build knowledge regarding whether and which miRNAs regulate FoxN1 gene in the aged thymus. We primarily compared changes in miRNA expression profile between young and aged murine TECs with Mus musculus miRBase-V20 arrays (containing 1892 unique probes), and clearly identified and validated that at least one miRNA, miR-125a-5p, was increased in aged thymus. Applying miR-125a-5p mimics was able to inhibit FoxN1 3′UTR luciferase activity in a 293T cell line and to suppress FoxN1 expression in murine TEC Z210 cells. Since a single miRNA can play a fine-tuning role to regulate expression of multiple genes and a single gene can be regulated by multiple miRNAs, our result adds a single miRNA, miR-125a-5p, into the panel of FoxN1-regulating miRNAs associated with thymic aging.

• Foxn1 gene
• Thymic aging
• microRNA-125a-5p
• negative fine tune

E2F transcription factor 3 (E2F3) is oncogenic in tumorigenesis. Alterations in E2F3 functions correspond with poor prognosis in various cancers, underscoring their status for the clinical cancer phenotype. Latest reports discovered intricate networks between microRNAs (miRNAs) and E2F3 in regulating the balance of these events, including proliferation, apoptosis, metastasis, as well as drug resistance. miRNAs are non-coding small RNAs which negatively regulate gene expressions post-transcriptionally mainly through 3′-UTR binding of target mRNAs. Increasing evidence shows that E2F3 can be activated/inhibited by numerous miRNAs whose dysregulation has been implicated in malignancy. In turn, miRNAs themselves can be transcriptionally regulated by E2F3, thus forming a negative feedback loop. These findings add a new challenging layer of complexity to E2F3 network. Current understanding of the reciprocal link between E2F3 and miRNAs in human cancers were summarized, which could help to develop potential therapeutic strategies.

• E2F3
• apoptosis
• metastasis
• microRNA
• proliferation

• downregulation
• gastric cancer
• microRNA-564
• microarray
• transcription factor E2F3

• cell function
• human brain microvessel endothelial cells
• miR-125a-5p
• oxidized low-density lipoprotein
• signal pathway

MiR-125a has been characterized as a tumor suppressor in several cancers. However, the role of miR-125a in cervical cancer is unknown. In this study, we found the expression of miR-125a was downregulated in cervical cancer patients, and negatively correlated with the tumor size, FIGO stage, and preoperative metastasis. Kaplan-Meier analysis showed that miR-125a expression predicted favorable outcome for cervical cancer patients. Dual luciferase assays identified the STAT3 gene as a novel direct target of miR-125a. Functional studies showed that miR-125a overexpression significantly suppressed the growth, invasion and epithelial-mesenchymal transition (EMT) of cervical cancer cells both in vitro and in vivo via decreasing STAT3 expression. Moreover, miR-125a conferred to G2/M cell cycle arrest, accompanied by inhibition of several G2/M checkpoint proteins. Mechanistically, inactivation of miR-125a during cervical carcinogenesis was caused by HPV suppression of p53 expression. Clinically, STAT3, the expression of which, predicted poorer outcome, was inversely correlated with miR-125a in cervical cancer. These data highlight the importance of miR-125a in the cell proliferation and progression of cervical cancer, and indicate that miR-125a may be a useful therapeutic target for cervical cancer.

• STAT3
• cell growth
• cervical cancer
• metastasis
• miR-125a

• biomarker
• diagnosis
• gastric cancer
• microRNAs
• prognosis

• Animals
• Apoptosis
• Biomarkers, Tumor
• Cell Cycle/genetics
• Cell Proliferation
• Early Detection of Cancer
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs/genetics
• Neoplasm Metastasis
• Prognosis
• Stomach Neoplasms/diagnosis
• Stomach Neoplasms/genetics
• Stomach Neoplasms/mortality
• Stomach Neoplasms/therapy

• Foxp3
• MiR-125a-5p
• Myasthenia gravis (MG)
• Thymoma

• Competing endogenous RNA
• Competitive endogenous RNAs networks
• Gastric cancer
• MicroRNA response elements
• Perturbation

• Animals
• Databases, Genetic
• Gene Expression Regulation, Neoplastic
• Gene Regulatory Networks
• Genetic Predisposition to Disease
• Humans
• MicroRNAs/genetics
• MicroRNAs/metabolism
• Phenotype
• RNA/genetics
• RNA/metabolism
• RNA, Circular
• RNA, Long Noncoding/genetics
• RNA, Long Noncoding/metabolism
• RNA, Neoplasm/genetics
• RNA, Neoplasm/metabolism
• Response Elements
• Stomach Neoplasms/genetics
• Stomach Neoplasms/metabolism
• Stomach Neoplasms/pathology

• nasopharyngeal carcinoma
• microrna-205
• tumor promoter
• tumor protein p53-inducible nuclear protein 1

• Adult
• Apoptosis
• Base Sequence
• Carcinogenesis/genetics
• Carcinogenesis/metabolism
• Carcinogenesis/pathology
• Carcinoma
• Carrier Proteins/genetics
• Carrier Proteins/metabolism
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Collagen/chemistry
• Diffusion Chambers, Culture
• Drug Combinations
• Female
• Gene Expression Regulation, Neoplastic
• Genes, Reporter
• Heat-Shock Proteins/genetics
• Heat-Shock Proteins/metabolism
• Humans
• Laminin/chemistry
• Luciferases/genetics
• Luciferases/metabolism
• Male
• MicroRNAs/genetics
• MicroRNAs/metabolism
• Middle Aged
• Molecular Sequence Data
• Nasopharyngeal Carcinoma
• Nasopharyngeal Neoplasms/genetics
• Nasopharyngeal Neoplasms/metabolism
• Nasopharyngeal Neoplasms/pathology
• Nasopharynx/metabolism
• Nasopharynx/pathology
• Neoplasm Staging
• Proteoglycans/chemistry
• Signal Transduction

• invasion
• metastasis-associated gene 1 (MTA1)
• miR-125a-3p
• non-small-cell lung cancer
• proliferation

• Carcinoma, Non-Small-Cell Lung/pathology
• Cell Line, Tumor
• Cell Proliferation
• Histone Deacetylases/genetics
• Humans
• Lung Neoplasms/pathology
• MicroRNAs/physiology
• Neoplasm Invasiveness
• Neoplasm Metastasis
• Repressor Proteins/genetics
• Trans-Activators

• E2F3
• miR-125a-5p, proliferation
• microRNAs

• 3' Untranslated Regions/genetics
• Animals
• Blotting, Western
• Cell Line
• Cell Proliferation/genetics
• Cyclin D1/genetics
• Cyclin D1/metabolism
• DNA Replication/genetics
• E2F3 Transcription Factor/genetics
• E2F3 Transcription Factor/metabolism
• G1 Phase Cell Cycle Checkpoints/genetics
• Gene Expression
• Gene Knockdown Techniques
• Mice
• MicroRNAs/genetics
• Microscopy, Fluorescence
• Mutation
• Myoblasts/cytology
• Myoblasts/metabolism
• RNA Interference
• Reverse Transcriptase Polymerase Chain Reaction

• Biomarker
• Colorectal adenoma
• Colorectal cancer
• miRNA

• Adult
• Aged
• Aged, 80 and over
• Computational Biology
• Disease Progression
• E2F Transcription Factors/genetics
• Female
• Gene Expression Regulation, Neoplastic
• Gene Regulatory Networks
• Humans
• Male
• MicroRNAs/genetics
• Middle Aged
• RNA, Messenger/genetics
• RNA, Messenger/metabolism
• Stomach Neoplasms/genetics
• Stomach Neoplasms/pathology

• Biomarkers, Tumor/genetics
• Disease Progression
• Gene Expression Regulation, Neoplastic
• Humans
• MicroRNAs/genetics
• Molecular Targeted Therapy
• Prognosis
• Stomach Neoplasms/diagnosis
• Stomach Neoplasms/genetics
• Stomach Neoplasms/therapy