|
1
|
Chiba T, Murata M, Kawano T, Hashizume M, Akahoshi T. Reflectance spectra analysis for mucous assessment. World J Gastrointest Oncol 2021; 13:822-834. [PMID: 34457188 PMCID: PMC8371524 DOI: 10.4251/wjgo.v13.i8.822] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 03/01/2021] [Revised: 04/26/2021] [Accepted: 07/09/2021] [Indexed: 02/06/2023] Open
Abstract
This review report represents an overview of research and development on medical hyperspectral imaging technology and its applications. Spectral imaging technology is attracting attention as a new imaging modality for medical applications, especially in disease diagnosis and image-guided surgery. Considering the recent advances in imaging, this technology provides an opportunity for two-dimensional mapping of oxygen saturation (SatO2) of blood with high accuracy, spatial spectral imaging, and its analysis and provides detection and diagnostic information about the tissue physiology and morphology. Multispectral imaging also provides information about tissue oxygenation, perfusion, and potential function during surgery. Analytical algorithm has been examined, and indication of accurate map of relative hemoglobin concentration and SatO2 can be indicated with preferable resolution and frame rate. This technology is expected to provide promising biomedical information in practical use. Several studies suggested that blood flow and SatO2 are associated with gastrointestinal disorders, particularly malignant tumor conditions. The use and analysis of spectroscopic images are expected to potentially play a role in the detection and diagnosis of these diseases.
Collapse
Affiliation(s)
- Toru Chiba
- Pentax_LifeCare, HOYA Corporation, Akishima-shi 196-0012, Tokyo, Japan
| | - Masaharu Murata
- Center for Advanced Medical Innovation, Kyushu University, Fukuoka-shi 812-8582, Fukuoka, Japan
| | - Takahito Kawano
- Center for Advanced Medical Innovation, Kyushu University, Fukuoka-shi 812-8582, Fukuoka, Japan
| | - Makoto Hashizume
- Center for Advanced Medical Innovation, Kyushu University, Fukuoka-shi 812-8582, Fukuoka, Japan
| | - Tomohiko Akahoshi
- Department of Disaster and Emergency Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka_shi 812-8582, Fukuoka, Japan
| |
Collapse
|
|
2
|
Tannert A, Ramoji A, Neugebauer U, Popp J. Photonic monitoring of treatment during infection and sepsis: development of new detection strategies and potential clinical applications. Anal Bioanal Chem 2017; 410:773-790. [PMID: 29214536 DOI: 10.1007/s00216-017-0713-z] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/20/2017] [Revised: 10/06/2017] [Accepted: 10/17/2017] [Indexed: 01/02/2023]
Abstract
Despite the strong decline in the infection-associated mortality since the development of the first antibiotics, infectious diseases are still a major cause of death in the world. With the rising number of antibiotic-resistant pathogens, the incidence of deaths caused by infections may increase strongly in the future. Survival rates in sepsis, which occurs when body response to infections becomes uncontrolled, are still very poor if an adequate therapy is not initiated immediately. Therefore, approaches to monitor the treatment efficacy are crucially needed to adapt therapeutic strategies according to the patient's response. An increasing number of photonic technologies are being considered for diagnostic purpose and monitoring of therapeutic response; however many of these strategies have not been introduced into clinical routine, yet. Here, we review photonic strategies to monitor response to treatment in patients with infectious disease, sepsis, and septic shock. We also include some selected approaches for the development of new drugs in animal models as well as new monitoring strategies which might be applicable to evaluate treatment response in humans in the future. Figure Label-free probing of blood properties using photonics.
Collapse
Affiliation(s)
- Astrid Tannert
- Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745, Jena, Germany
- Jena Biophotonics and Imaging Laboratory, 07745, Jena, Germany
| | - Anuradha Ramoji
- Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745, Jena, Germany
- Center for Sepsis Control and Care, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany
| | - Ute Neugebauer
- Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745, Jena, Germany.
- Jena Biophotonics and Imaging Laboratory, 07745, Jena, Germany.
- Center for Sepsis Control and Care, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany.
- Institute of Physical Chemistry, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
- InfectoGnostics Research Campus Jena, Philosophenweg 7, Jena, Germany.
| | - Jürgen Popp
- Leibniz Institute of Photonic Technology, Albert-Einstein-Str. 9, 07745, Jena, Germany
- Jena Biophotonics and Imaging Laboratory, 07745, Jena, Germany
- Center for Sepsis Control and Care, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany
- Institute of Physical Chemistry, Friedrich Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany
- InfectoGnostics Research Campus Jena, Philosophenweg 7, Jena, Germany
| |
Collapse
|
|
3
|
Saraví FD, Carra GE, Matus DA, Ibáñez JE. Rectification of oxygen transfer through the rat colonic epithelium. World J Gastrointest Pathophysiol 2017; 8:59-66. [PMID: 28573068 PMCID: PMC5437503 DOI: 10.4291/wjgp.v8.i2.59] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/26/2017] [Revised: 03/24/2017] [Accepted: 04/24/2017] [Indexed: 02/06/2023] Open
Abstract
AIM To assess whether higher sensitivity of colonic epithelium to hypoxia at the serosal side is associated with oxygen transfer asymmetry.
METHODS Rats were fed either with normal chow or a low-sodium diet. Tissues were mounted as flat sheets in a modified, airtight Ussing chamber with oxygen meters in each hemichamber. Mucosal samples from normal diet animals were studied under control conditions, in low-chloride solution and after adding chloride secretion inhibitors and chloride secretagogues. Samples from sodium-deprived rats were studied before and after ouabain addition. In separate experiments, the correlation between short-circuit current and oxygen consumption was analyzed. Finally, hypoxia was induced in one hemichamber to assess the relationship between its oxygen content and the oxygen pressure difference between both hemichambers.
RESULTS In all studied conditions, oxygen consumption was larger in the serosal hemichamber than in the mucosal one (P = 0.0025 to P < 0.0001). Short-circuit current showed significant correlation with both total oxygen consumption (r = 0.765; P = 0.009) in normoxia and oxygen consumption in the serosal hemichamber (r = 0.754; P = 0.011) during mucosal hypoxia, but not with oxygen consumption in the mucosal hemichamber. When hypoxia was induced in the mucosal hemichamber, an oxygen pressure difference of 13 kPa with the serosal hemichamber was enough to keep its oxygen content constant. However, when hypoxia was induced in the serosal hemichamber, the oxygen pressure difference with the mucosal hemichamber necessary to keep its oxygen content constant was 40 kPa (P < 0.0001).
CONCLUSION Serosal oxygen supply is more readily available to support short-circuit current. This may be partly due to a rectifying behavior of transepithelial oxygen transfer.
Collapse
|
|
4
|
van Noord D, Kolkman JJ. Functional testing in the diagnosis of chronic mesenteric ischemia. Best Pract Res Clin Gastroenterol 2017; 31:59-68. [PMID: 28395789 DOI: 10.1016/j.bpg.2016.12.002] [Citation(s) in RCA: 15] [Impact Index Per Article: 1.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/10/2016] [Revised: 12/03/2016] [Accepted: 12/17/2016] [Indexed: 01/31/2023]
Abstract
Chronic mesenteric ischemia (CMI) results from insufficient oxygen delivery or utilization to meet metabolic demand. Two main mechanisms may lead to mesenteric ischemia: occlusion in the arteries or veins of the gastrointestinal tract, or reduced blood flow from shock states or increased intra-abdominal pressure, so-called non-occlusive mesenteric ischemia. Severe stenoses in the three main mesenteric vessels as demonstrated with CT-angiography or MR-angiography are sufficient to proof mesenteric ischemia, for example in patients who present with weight loss, postprandial pain and diarrhea. Still in many clinical situations mesenteric ischemia is only one of many possible explanations. Especially in patients with a single vessel stenosis in the celiac artery or superior mesenteric artery with postprandial pain, mesenteric ischemia remains a diagnosis of probability or assumption without functional proof of actual ischemia. This review is aimed to provide an overview of all past, present and future ways to functionally proof CMI.
Collapse
Affiliation(s)
- Desirée van Noord
- Erasmus MC University Medical Center Rotterdam, Department of Gastroenterology and Hepatology, Rotterdam, The Netherlands; Franciscus Gasthuis & Vlietland, Department of Gastroenterology and Hepatology, Rotterdam, The Netherlands.
| | - Jeroen J Kolkman
- Medisch Spectrum Twente, Department of Gastroenterology and Hepatology, Enschede, The Netherlands; Universitair Medisch Centrum Groningen, Department of Gastroenterology and Hepatology, Groningen, The Netherlands.
| |
Collapse
|
|
5
|
Jung DS, Crowe JA, Birchall JP, Somekh MG, See CW. Anti-confocal assessment of middle ear inflammation. BIOMEDICAL OPTICS EXPRESS 2017; 8:230-242. [PMID: 28101414 PMCID: PMC5231295 DOI: 10.1364/boe.8.000230] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Subscribe] [Scholar Register] [Received: 09/20/2016] [Revised: 11/03/2016] [Accepted: 11/04/2016] [Indexed: 06/06/2023]
Abstract
To improve the diagnostic prediction of recurrence of otitis media with effusion after surgery, an anti-confocal system combined with spectroscopic measurements is proposed to reject unwanted signals from the eardrum and assess the blood content. The anti-confocal system was experimentally evaluated on both optical middle ear phantom and human skin. Results showed effective rejection of signals from the eardrum using a central stop replacing the confocal pinhole, while still detecting signals from the middle ear mucosa. The system is sensitive to changes in blood content, but scattering and absorption characteristics of the eardrum can distort the measurement. Confocal detection of eardrum properties was shown to be a promising approach to correct measurements.
Collapse
Affiliation(s)
- David S. Jung
- National Institute for Health Research (NIHR) Nottingham Hearing Biomedical Research Unit, 113 The Ropewalk, Nottingham, NG1 5DU,
UK
- Department of Electrical and Electronic Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD,
UK
| | - John A. Crowe
- Department of Electrical and Electronic Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD,
UK
| | - John P. Birchall
- National Institute for Health Research (NIHR) Nottingham Hearing Biomedical Research Unit, 113 The Ropewalk, Nottingham, NG1 5DU,
UK
- Otology and Hearing group, Division of Clinical Neuroscience, School of Medicine, University of Nottingham, Nottingham, NG7 2UH,
UK
| | - Michael G. Somekh
- Department of Electrical and Electronic Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD,
UK
- Department of Electronic and Information Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong,
China
| | - Chung W. See
- Department of Electrical and Electronic Engineering, University of Nottingham, University Park, Nottingham, NG7 2RD,
UK
| |
Collapse
|
|
6
|
Zuo GW, Liang LX, Yao P, Huang D, Liu X, He WR, Zhang FC. Prevalence and risk factors for functional dyspepsia in university students in Nanning. Shijie Huaren Xiaohua Zazhi 2014; 22:263-267. [DOI: 10.11569/wcjd.v22.i2.263] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 02/06/2023] Open
Abstract
AIM: To find the risk factors for functional dyspepsia (FD) in different populations.
METHODS: Cluster random sampling method was used to select university students in Nanning, and data were collected by face-to-face interview according to Rome III Diagnostic Questionnaire for Adult FGID. FD was diagnosed according to the scoring algorithm for the Rome III Diagnostic Questionnaire for Adult FGIDs. The prevalence of FD was accessed and the risk factors for FD were analyzed.
RESULTS: A total of 2580 questionnaires were administered and 2520 were returned with a response rate of 97.7%. The overall prevalence of FD among university students in Nanning was 5.4%. The prevalence of FD differed by gender, with a higher prevalence in females than in males (5.91% vs 3.84%). No midnight snack, less exercise time and sleeping time were found to be correlated with FD by multiple logistic regression analysis (P < 0.05 for all).
CONCLUSION: FD was a common disease in university students in Nanning, with an overall prevalence of 5.4%. The factors that affect this disease mainly included gender, midnight snack, exercise time and sleeping time.
Collapse
|
|
7
|
Carra GE, Ibáñez JE, Saraví FD. The effect of acute hypoxia on short-circuit current and epithelial resistivity in biopsies from human colon. Dig Dis Sci 2013; 58:2499-506. [PMID: 23695875 DOI: 10.1007/s10620-013-2711-0] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 12/15/2012] [Accepted: 05/02/2013] [Indexed: 12/09/2022]
Abstract
BACKGROUND AND AIMS In isolated colonic mucosa, decreases in short-circuit current (ISC) and transepithelial resistivity (RTE) occur when hypoxia is either induced at both sides or only at the serosal side of the epithelium. We assessed in human colon biopsies the sensitivity to serosal-only hypoxia and mucosal-only hypoxia and whether Na, K-ATPase blockade with ouabain interacts with hypoxia. MATERIALS AND METHODS Biopsy material from patients undergoing colonoscopy was mounted in an Ussing chamber for small samples (1-mm2 window). In a series of experiments we assessed viability and the electrical response to the mucolytic, dithiothreitol (1 mmol/l). In a second series, we explored the effect of hypoxia without and with ouabain. In a third series, we evaluated the response to a cycle of hypoxia and reoxygenation induced at the serosal or mucosal side while keeping the oxygenation of the opposite side. RESULTS 1st series: Dithiothreitol significantly decreased the unstirred layer and ISC but increased RTE. 2nd series: Both hypoxia and ouabain decreased ISC, but ouabain increased RTE and this effect on RTE prevailed even during hypoxia. 3rd series: Mucosal hypoxia caused lesser decreases of ISC and RTE than serosal hypoxia; in the former, but not in the latter, recovery was complete upon reoxygenation. CONCLUSIONS In mucolytic concentration, dithiothreitol modifies ISC and RTE. Oxygen supply from the serosal side is more important to sustain ISC and RTE in biopsy samples. The different effect of hypoxia and Na, K-ATPase blockade on RTE suggests that their depressing effect on ISC involves different mechanisms.
Collapse
Affiliation(s)
- Graciela E Carra
- Faculty of Medical Sciences, Institute of Physiology, Universidad Nacional de Cuyo, 5500, Mendoza, Argentina.
| | | | | |
Collapse
|
|
8
|
Venous small bowel infarction: intraoperative laser Doppler flowmetry discriminates critical blood supply and spares bowel length. Case Rep Med 2012; 2012:195926. [PMID: 23093968 PMCID: PMC3475002 DOI: 10.1155/2012/195926] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/15/2012] [Accepted: 09/23/2012] [Indexed: 01/07/2023] Open
Abstract
Introduction. In mesenteric infarction due to arterial occlusion, laser Doppler flowmetry and spectrometry are known reliable noninvasive methods for measuring microvascular blood flow and oxygen utilisation. Case Presentation. As an innovation we used these methods in a patient with acute extensive mesenteric infarction due to venous occlusion, occurring after radical right hemicolectomy. Aiming to avoid short bowel syndrome, we spared additional 110 cm of small bowel, instead of leaving only 80 centimetres of clinically viable small bowel in situ. The pathological examination showed only 5 mm of vital mucosa to be left distal to the dissection margin. No further interventions were necessary. Conclusion. Laser doppler flowmetry and spectrometry are potentially powerful methods to assist the surgeon's decision-making in critical venous mesenteric perfusion, thus having an important impact on clinical outcome.
Collapse
|
|
9
|
Electrogenic transport, oxygen consumption, and sensitivity to acute hypoxia of human colonic epithelium. Int J Colorectal Dis 2011; 26:1205-10. [PMID: 21519802 DOI: 10.1007/s00384-011-1215-7] [Citation(s) in RCA: 12] [Impact Index Per Article: 0.9] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Accepted: 04/11/2011] [Indexed: 02/04/2023]
Abstract
INTRODUCTION It is recognized that epithelial ion transport depends on oxygen supply, but this dependence has not been characterized in the human colon in vitro despite its surgical and clinical implications. PURPOSES The aim of this study is to measure the oxygen consumption of colonic epithelium under conditions which preserve vectorial ion transport and to assess the sensitivity of the human colonic epithelium short-circuit current (I (sc)) to acute hypoxia induced in vitro. METHODS Isolated mucosa preparations from human sigmoid colon were placed in a modified Ussing chamber which allows simultaneous measurement of short-circuit current (I (sc)) and oxygen consumption (QO(2)). In separate experiments, the sensitivity to acute hypoxia induced in a conventional Ussing chamber was assessed. RESULTS Basal mean ± SEM values (n = 8) were I (sc) = 3.3 ± 0.5 μEq h(-1) cm(-2) and QO(2) = 8.09 ± 0.55 μmol h(-1) cm(-2). The contribution of the serosal side to the oxygen supply was higher than that of the mucosal side (p = 0.0023). Ouabain reduced I (sc) by 70% (P < 0.0001) and QO(2) by 26% (n = 8; P = 0.0009), suggesting that a large fraction of QO(2) is needed to support ouabain-sensitive electrogenic transport. Induction of hypoxia at both sides of the Ussing chamber caused a rapid decrease in I (sc) after 2 min. I (sc) was also significantly depressed when hypoxia was induced by 5 min in the serosal side (n = 6, P < 0.0001), but was unaffected by hypoxia induced in the mucosal side. CONCLUSION The present results allow a better understanding of the clinical consequences of acute hypoxia on intestinal ion transport.
Collapse
|
|
10
|
Leung FW. Search for premalignant mucosal lesions: does endoscopic measurement of oxygen saturation by differential path-length spectroscopy help? Gastrointest Endosc 2009; 70:7-8. [PMID: 19559830 DOI: 10.1016/j.gie.2008.11.015] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.1] [Reference Citation Analysis] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 10/28/2008] [Accepted: 11/09/2008] [Indexed: 12/10/2022]
|
|
11
|
Thorn CE, Matcher SJ, Meglinski IV, Shore AC. Is mean blood saturation a useful marker of tissue oxygenation? Am J Physiol Heart Circ Physiol 2009; 296:H1289-95. [DOI: 10.1152/ajpheart.01192.2008] [Citation(s) in RCA: 43] [Impact Index Per Article: 2.7] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Increasingly we are monitoring the distribution of oxygen through the microcirculation using optical techniques such as optical reflectance spectroscopy (ORS) and near-infrared spectroscopy. Mean blood oxygen saturation (SmbO2) and tissue oxygenation index measured by these two techniques, respectively, evoke a concept of the measurement of oxygen delivery to tissue. This study aims to establish whether SmbO2 is an appropriate indicator of tissue oxygenation. Spontaneous fluctuations in SmbO2 observed as changes in concentration of oxyhemoglobin ([HbO2]) and deoxyhemoglobin ([Hb]) were measured by ORS in the skin microcirculation of 30 healthy subjects (15 men, age 21–42 yr). Fourier analysis identified two distinctly different spontaneous falls in SmbO2. The first type of swing, thought to be induced by fluctuations in arterial blood volume, resulted from the effects of respiration, endothelial, sympathetic, and myogenic activity. There was no apparent change in [Hb]. In contrast, a second type of swing resulted from a fall in [HbO2] accompanied by a rise in [Hb] and was only induced by endothelial and sympathetic activity. Thus the same fall in SmbO2 can be induced by two distinct responses. A “type I” swing does not suggest an inadequacy in oxygen delivery whereas a “type II” swing may indicate a change in oxygen delivery from blood to tissue. SmbO2 alone cannot therefore be accepted as a definitive marker of tissue oxygenation.
Collapse
|