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Nonković D, Tešić V, Šimat V, Karabuva S, Medić A, Hrabar J. Anisakidae and Anisakidosis: A Public Health Perspective. Pathogens 2025; 14:217. [PMID: 40137702 PMCID: PMC11944503 DOI: 10.3390/pathogens14030217] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/28/2025] [Revised: 02/16/2025] [Accepted: 02/19/2025] [Indexed: 03/29/2025] Open
Abstract
Fish and seafood are increasingly recognised as safe and nutritiously valuable foods of animal origin, being a source of about 17% of animal protein globally. Novel culinary trends encourage the consumption of raw or thermally lightly processed fishery products. At the same time, consumers prefer wild, fresh and whole fish over farmed or processed fish. However, the consumption of raw or undercooked fish and other marine organisms poses a risk of contracting parasitic infections, potentially representing a public health risk. Among the most common seafoodborne parasites are members of the Anisakidae family, especially the genus Anisakis, which can cause potentially detrimental effects to human health. These parasites are the causative agent of a zoonosis termed anisakidosis that is prevalent in countries with high per capita fish consumption. Although the number of annual clinical cases varies among countries and regions and is generally not high, sensitisation to this parasite in the general population seems to be considerably higher. Therefore, anisakidosis is still significantly underreported and misdiagnosed globally, making it a disease of rising public health concerns. To prevent infection and mitigate potential negative effects on human health, proper preventive measures such as gutting the fish, freezing or thermal processing are needed. Moreover, a holistic approach implementing One Health principles together with educational campaigns towards the general public and primary care physicians can extend the knowledge on the occurrence of these parasites in their natural hosts and the diagnosis and incidence of anisakidosis, with a final goal to minimize risks for human health and reducing costs for health systems.
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Affiliation(s)
- Diana Nonković
- Department of Epidemiology, Teaching Institute of Public Health of Split-Dalmatia County, 21000 Split, Croatia;
- University Department of Health Studies, University of Split, 21000 Split, Croatia
| | - Vanja Tešić
- Department of Epidemiology, Teaching Institute of Public Health “Dr. Andrija Štampar”, 10000 Zagreb, Croatia;
- Department of Social Medicine and Epidemiology, Faculty of Medicine, University of Rijeka, 51000 Rijeka, Croatia
| | - Vida Šimat
- University Department of Marine Studies, University of Split, 21000 Split, Croatia;
| | - Svjetlana Karabuva
- Infectious Diseases Department, University Hospital of Split, 21000 Split, Croatia;
| | - Alan Medić
- Department of Epidemiology, Zadar Institute of Public Health, 23000 Zadar, Croatia;
- Department of Health Studies, University of Zadar, 23000 Zadar, Croatia
| | - Jerko Hrabar
- Laboratory of Aquaculture, Institute of Oceanography and Fisheries, 21000 Split, Croatia
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Hamamoto H, Matsuda T, Endo M. A Rare Subepithelial Lesion of the Proximal Colon Evaluated Using Miniprobe Endoscopic Ultrasonography: A Case Report. Cureus 2025; 17:e79793. [PMID: 40161107 PMCID: PMC11954684 DOI: 10.7759/cureus.79793] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Accepted: 02/27/2025] [Indexed: 04/02/2025] Open
Abstract
Preoperative diagnosis of submucosal lesions in the colon can be challenging. Total colonoscopy comprising miniprobe endoscopic ultrasonography (mEUS) revealed a 5-mm submucosal lesion in the transverse colon as a hypoechoic area primarily within the submucosa in our patient. However, a definitive diagnosis could not be made after evaluating the biopsy results. Based on the mEUS findings, adhesion to the muscularis was localized; therefore, endoscopic submucosal dissection was performed. A postoperative pathology examination revealed a parasite-like structure at the center of the necrotic tissue that was diagnosed as a colorectal-type necrotic nodule. Diagnostic EUS should be considered for such cases, and mEUS is useful for assessing the safety of specimen collection. This technique is especially valuable for diagnosing colorectal submucosal lesions in the proximal colon, where other diagnostic modalities are limited, and may contribute to safer endoscopic mucosal resection and endoscopic submucosal dissection.
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Affiliation(s)
| | - Tomoki Matsuda
- Department of Gastroenterology, Sendai Kousei Hospital, Sendai, JPN
| | - Mareyuki Endo
- Department of Surgical Pathology, Sendai Kousei Hospital, Sendai, JPN
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Syed S, Ortega S, Anderssen KE, Nilsen HA, Heia K. Hyperspectral imaging and deep learning for parasite detection in white fish under industrial conditions. Sci Rep 2024; 14:27426. [PMID: 39521834 PMCID: PMC11550473 DOI: 10.1038/s41598-024-76808-w] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/15/2024] [Accepted: 10/16/2024] [Indexed: 11/16/2024] Open
Abstract
Parasites in fish muscle present a significant problem for the seafood industry in terms of both quality and health and safety, but the low contrast between parasites and fish tissue makes them exceedingly difficult to detect. The traditional method to identify nematodes requires removing fillets from the production line for manual inspection on candling tables. This technique is slow, labor intensive and typically only finds about half the parasites present. The seafood industry has struggled for decades to develop a method that can improve the detection rate while being performed in a rapid, non-invasive manner. In this study, a newly developed solution uses deep neural networks to simultaneously analyze the spatial and spectral information of hyperspectral imaging data. The resulting technology can be directly integrated into existing industrial processing lines to rapidly identify nematodes at detection rates (73%) better than conventional manual inspection (50%).
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Affiliation(s)
- Shaheen Syed
- Department of Seafood Industry, Nofima AS, P.O. Box 6122, 9291, Tromsö, Norway
- Department of Computer Science, UiT, The Arctic University of Norway, Hansine Hansens Veg 18, 9009, Tromsö, Norway
| | - Samuel Ortega
- Department of Seafood Industry, Nofima AS, P.O. Box 6122, 9291, Tromsö, Norway
| | - Kathryn E Anderssen
- Department of Seafood Industry, Nofima AS, P.O. Box 6122, 9291, Tromsö, Norway.
| | - Heidi A Nilsen
- Department of Seafood Industry, Nofima AS, P.O. Box 6122, 9291, Tromsö, Norway
| | - Karsten Heia
- Department of Seafood Industry, Nofima AS, P.O. Box 6122, 9291, Tromsö, Norway
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Ito K, Ide T, Tanaka T, Nagayasu E, Hasegawa H, Noshiro H. A surgical case of inflammatory pseudotumor by hepatic anisakiasis. Clin J Gastroenterol 2024; 17:143-147. [PMID: 38085443 DOI: 10.1007/s12328-023-01891-x] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 08/22/2023] [Accepted: 10/29/2023] [Indexed: 02/01/2024]
Abstract
Anisakiasis is a parasitic infection caused by the ingestion of raw or undercooked seafood infected with Anisakis larvae. It generally affects the gastrointestinal tract, particularly the stomach, but very rare cases have been reported in which infection of the liver leads to the formation of inflammatory pseudotumors. We herein report an extremely rare case of an inflammatory pseudotumor induced by hepatic anisakiasis that was laparoscopically resected for the purpose of both diagnosis and treatment. A 51-year-old woman underwent a routine medical checkup by ultrasound examination, which incidentally detected a 15-mm mass on the surface of S6 of the liver. Because a malignant tumor could not be ruled out on several preoperative imaging studies, laparoscopic partial resection of the liver was performed. Histopathological examination revealed Anisakis larva in the inflammatory pseudotumor, suggesting hepatic anisakiasis. This report describes an extremely rare case of an inflammatory pseudotumor induced by hepatic anisakiasis. Because the preoperative diagnosis could not be obtained by several imaging modalities, laparoscopic liver resection with a sufficient margin might be suitable for diagnosis and treatment of this disease.
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Affiliation(s)
- Kotaro Ito
- Faculty of Medicine, Department of Surgery, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan.
| | - Takao Ide
- Faculty of Medicine, Department of Surgery, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan
| | - Tomokazu Tanaka
- Faculty of Medicine, Department of Surgery, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan
| | - Eiji Nagayasu
- Faculty of Medicine, Division of Parasitology, Department of Infectious Diseases, University of Miyazaki, 5200 Kiyotakecho Kihara, Miyazaki-City, Miyazaki, 889-1692, Japan
| | - Hideo Hasegawa
- Faculty of Medicine, Department of Biomedicine, Oita University, 1-1 Idaigaoka, Hasama, Yufu, Oita, 879-5593, Japan
| | - Hirokazu Noshiro
- Faculty of Medicine, Department of Surgery, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan
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Chen Zhou ZH, Ayala Calvo G, Martínez Chamorro E, Ibáñez Sanz L, Sanz de Lucas R, Borruel Nacenta S. Intestinal anisakiasis: CT imaging findings-a 10-year descriptive study in a single center. Emerg Radiol 2023; 30:621-627. [PMID: 37612541 DOI: 10.1007/s10140-023-02166-1] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/11/2023] [Accepted: 08/11/2023] [Indexed: 08/25/2023]
Abstract
PURPOSE The aim of this study was to retrospectively review cases of intestinal anisakiasis diagnosed by CT over a 10-year period and to evaluate imaging findings associated with the disease. METHODS This retrospective study included 71 patients with clinical suspicion of intestinal anisakiasis in whom an abdominopelvic computed tomography (CT) was performed at a single institution between June 2011 and December 2021. To identify the cases, we used medical term search engines and the hospital's radiology case database. Clinical information was gathered from the medical records. A radiologist with five years of experience reviewed and analyzed the CT images to determine the characteristic findings of intestinal anisakiasis. RESULTS The study included 47 confirmed cases of intestinal anisakiasis. The mean age of the patients was 52 years (range 18-87 years), being more frequent in men than women (26:21). All patients reported ingestion of raw fish, most commonly anchovies in vinegar (30/47, 63,8%). Abdominal pain was the predominant symptom, accompanied by nausea, vomiting, and occasionally fever. The most common clinical suspicions were intestinal obstruction (14/47, 29,8%) and appendicitis (10/47, 21,3%), whereas intestinal anisakiasis was suspected in only 2 cases prior to imaging. CT showed thickening of the bowel wall with submucosal edema in all patients, predominantly involving the ileum (43/47, 91,5%), usually in a relatively long segment (mean of 17,5 cm, range 10-30 cm). Simultaneous involvement of multiple bowel segments was observed in 16 cases (34%). Intestinal obstruction with dilatation of proximal loops (33/47, 70,2%), ascites (45/47, 95,7%), and mesenteric fat striation (32/47, 68,1%) were also common findings. CONCLUSION This study demonstrates the value of computed tomography in suggesting the diagnosis of intestinal anisakiasis, which often presents with nonspecific clinical manifestations. The characteristic CT findings that provide diagnostic clues are bowel wall thickening with submucosal edema, typically involving a long segment of the ileum, with signs of intestinal obstruction, ascites, and mesenteric fat striation. Simultaneous involvement of several intestinal segments (typically the gastric antrum and right colon) is an additional finding to be considered and may provide a diagnostic clue.
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Affiliation(s)
- Zhao Hui Chen Zhou
- Department of Radiology, Hospital Universitario 12 de Octubre, Madrid, Spain.
| | - Gerardo Ayala Calvo
- Department of Radiology, Hospital Universitario 12 de Octubre, Madrid, Spain
| | | | - Laín Ibáñez Sanz
- Department of Radiology, Hospital Universitario 12 de Octubre, Madrid, Spain
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Brusca I, Barrale M, Zarcone M, Fruscione S, Onida R, De Bella DD, Alba D, Belluzzo M, Uasuf CG, Cammilleri G, Costa A, Ferrantelli V, Savatteri A, Cannizzaro E, Calamusa G, Lacca G, Maida CM, Pipitone S, D’Atria A, Palomba M, Costantino C, Mattiucci S, Mazzucco W. Basophil Activation Test in the Diagnosis of Anisakis Allergy: An Observational Study from an Area of High Seafood Consumption in Italy. Pathogens 2023; 12:777. [PMID: 37375467 PMCID: PMC10305353 DOI: 10.3390/pathogens12060777] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/03/2023] [Revised: 05/23/2023] [Accepted: 05/25/2023] [Indexed: 06/29/2023] Open
Abstract
The rising popularity of undercooked or raw seafood containing larvae of the Anisakis parasite has led to issues of public health concern due to allergic manifestations. We conducted an observational study on the use of an innovative Anisakis allergy diagnostic algorithm in a convenience sample of 53 allergic outpatients recruited in Western Sicily, between April 2021 and March 2022. We included individuals with an anamnesis suggestive of IgE sensitization to Anisakis reporting clinical manifestation in the last month due to allergic reactions after eating fresh fish, or in subjects at high exposure risk with sea products while abstaining from fish ingestion, excluding those with documented fish sensitization. Outpatients were tested via Skin Prick Test, IgE-specific dosage and Basophil Activation Test (BAT). Twenty-six outpatients were diagnosed with Anisakis, while 27 with Chronic Urticaria (CU). We found a seven-fold excess risk for Anisakis (p4) positivity in the Anisakis allergic outpatients, as compared to the CU ones. BAT showed the best diagnostic accuracy (92.45%) and specificity (100%), while specific IgE to Ascaris (p1) documented the best sensitivity (92.31%) but a very low specificity (37.04%). In conclusion, our findings may represent a potentially useful contribution to the future development of updated clinical guidelines.
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Affiliation(s)
- Ignazio Brusca
- U.O.C of Clinical Pathology Buccheri La Ferla Hospital FBF, 90123 Palermo, Italy; (I.B.); (M.B.); (R.O.)
| | - Maria Barrale
- U.O.C of Clinical Pathology Buccheri La Ferla Hospital FBF, 90123 Palermo, Italy; (I.B.); (M.B.); (R.O.)
| | - Maurizio Zarcone
- U.O.C. of Clinical Epidemiology with Cancer Registry, Azienda Ospedaliera Universitaria Policlinico di Palermo, 90127 Palermo, Italy; (M.Z.); (S.P.); (A.D.); (W.M.)
| | - Santo Fruscione
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Rosa Onida
- U.O.C of Clinical Pathology Buccheri La Ferla Hospital FBF, 90123 Palermo, Italy; (I.B.); (M.B.); (R.O.)
| | - Daniele Domenico De Bella
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Davide Alba
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Miriam Belluzzo
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Carina Gabriela Uasuf
- Allergy Disease Center, Institute of Translational Pharmacology (IFT), National Research Council (CNR), 90146 Palermo, Italy;
| | - Gaetano Cammilleri
- Experimental Zooprophylactic Institute of Sicily, 90129 Palermo, Italy; (G.C.); (A.C.); (V.F.)
| | - Antonella Costa
- Experimental Zooprophylactic Institute of Sicily, 90129 Palermo, Italy; (G.C.); (A.C.); (V.F.)
| | - Vincenzo Ferrantelli
- Experimental Zooprophylactic Institute of Sicily, 90129 Palermo, Italy; (G.C.); (A.C.); (V.F.)
| | - Alessandra Savatteri
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Emanuele Cannizzaro
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Giuseppe Calamusa
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Guido Lacca
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Carmelo Massimo Maida
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Salvatore Pipitone
- U.O.C. of Clinical Epidemiology with Cancer Registry, Azienda Ospedaliera Universitaria Policlinico di Palermo, 90127 Palermo, Italy; (M.Z.); (S.P.); (A.D.); (W.M.)
| | - Alida D’Atria
- U.O.C. of Clinical Epidemiology with Cancer Registry, Azienda Ospedaliera Universitaria Policlinico di Palermo, 90127 Palermo, Italy; (M.Z.); (S.P.); (A.D.); (W.M.)
| | - Marialetizia Palomba
- Department of Ecological and Biological Sciences, Tuscia University, 01100 Viterbo, Italy;
| | - Claudio Costantino
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
| | - Simonetta Mattiucci
- Department of Public Health and Infectious Diseases, Section of Parasitology, Sapienza-University of Rome, University Hospital “Policlinico Umberto I”, 00185 Rome, Italy;
| | - Walter Mazzucco
- U.O.C. of Clinical Epidemiology with Cancer Registry, Azienda Ospedaliera Universitaria Policlinico di Palermo, 90127 Palermo, Italy; (M.Z.); (S.P.); (A.D.); (W.M.)
- PROMISE Department, University of Palermo, 90127 Palermo, Italy; (D.D.D.B.); (D.A.); (M.B.); (A.S.); (E.C.); (G.C.); (G.L.); (C.M.M.); (C.C.)
- College of Medicine, University of Cincinnati, Cincinnati, OH 45267, USA
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Gastric Anisakiasis Masquerading as Gastroesophageal Reflux Disease. Case Rep Gastrointest Med 2023; 2023:8635340. [PMID: 36818745 PMCID: PMC9931486 DOI: 10.1155/2023/8635340] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Track Full Text] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 11/04/2022] [Revised: 01/28/2023] [Accepted: 01/30/2023] [Indexed: 02/11/2023] Open
Abstract
Anisakiasis of the gastrointestinal tract is caused by the consumption of raw or undercooked seafood infected with Anisakis larvae. Penetration of Anisakis larvae into the gastrointestinal mucosa leads to severe epigastric pain, nausea, and vomiting, usually within hours of ingestion of the parasite. Suspicion for gastrointestinal Anisakiasis should be raised in patients with a compatible dietary history. Definitive diagnosis can be made by direct visualization of larvae via endoscopic examination. Although symptoms are self-limiting, the removal of larvae by gastroscopy can hasten relief of symptoms. There are a large number of cases of gastric anisakiasis reported from Japan, Korea, and Western Europe, where it is customary to consume raw fish. Cases reported from the United States are less common, and given the nonspecific symptoms of anisakiasis, the diagnosis can be missed. We report a patient who presented with gastroesophageal reflux disease like symptoms that started after ingesting raw fish. He was found by esophagogastroduodenoscopy to have white, filiform worms penetrating into the wall of his stomach, consistent with a diagnosis of gastric anisakiasis.
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Ishizaki Y, Kawashima K, Gunji N, Onizawa M, Hikichi T, Hasegawa M, Ohira H. Trichuris trichiura Incidentally Detected by Colonoscopy and Identified by a Genetic Analysis. Intern Med 2022; 61:821-825. [PMID: 34471029 PMCID: PMC8987263 DOI: 10.2169/internalmedicine.8012-21] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Indexed: 12/04/2022] Open
Abstract
Although trichuriasis, a zoonotic disease, has recently become rare in Japan due to improved environmental hygiene, we herein report a 79-year-old man in whom a worm was incidentally found in the ascending colon during colonoscopy for positive fecal occult blood and was endoscopically removed. A genetic analysis identified the worm as Trichuris trichiura possessing mixed sequences from non-human primate and human origins. Despite controversy regarding Trichuris trichiura infection originating from Japanese macaques, according to some studies, it originates primarily from humans. This report suggests the efficacy of a genetic analysis for identifying infection sources.
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Affiliation(s)
- Yuto Ishizaki
- Department of Gastroenterology, Fukushima Medical University School of Medicine, Japan
| | - Kazumasa Kawashima
- Department of Gastroenterology, Fukushima Medical University School of Medicine, Japan
| | - Naohiko Gunji
- Department of Gastroenterology, Fukushima Medical University School of Medicine, Japan
| | - Michio Onizawa
- Department of Gastroenterology, Fukushima Medical University School of Medicine, Japan
| | - Takuto Hikichi
- Department of Endoscopy, Fukushima Medical University Hospital, Japan
| | - Mitsuko Hasegawa
- Laboratory of Helminthology, Department of Parasitology, National Institute of Infectious Diseases, Japan
- Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Japan
| | - Hiromasa Ohira
- Department of Gastroenterology, Fukushima Medical University School of Medicine, Japan
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Anisakis, Something Is Moving inside the Fish. Pathogens 2022; 11:pathogens11030326. [PMID: 35335650 PMCID: PMC8950136 DOI: 10.3390/pathogens11030326] [Citation(s) in RCA: 7] [Impact Index Per Article: 2.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/26/2022] [Revised: 03/03/2022] [Accepted: 03/04/2022] [Indexed: 02/06/2023] Open
Abstract
The first case of human infection by a species of the Anisakidae family was reported more than 60 years ago. Over the last 20 years, Anisakis has become a highly studied parasite, not only for its parasitism, but also for its role as an inducer of allergic reactions. Several studies have indicated that the pathological changes occurring within the gastrointestinal tract during infection with Anisakis simplex are the combined result of the direct action of the larvae invading the tissue and the complex interaction between the host’s immune system and the parasite. Although the most commonly described pathologies are digestive, urticaria/angioedema and anaphylaxis, occupational asthma and arthritis have been seldom described. This paper is a narrative of the immune-mediated reaction induced by this parasite over the course of the last two decades.
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Grillo F, Campora M, Carlin L, Cornara L, Parente P, Vanoli A, Remo A, Migliora P, Roberto F, Fassan M, Mastracci L. "Stranger things" in the gut: uncommon items in gastrointestinal specimens. Virchows Arch 2022; 480:231-245. [PMID: 34599376 PMCID: PMC8986674 DOI: 10.1007/s00428-021-03188-1] [Citation(s) in RCA: 6] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/11/2021] [Revised: 08/05/2021] [Accepted: 08/11/2021] [Indexed: 12/14/2022]
Abstract
Organic (such as parasites or vegetable remnants) and inorganic substances may be encountered during routine pathology diagnostic work up of endoscopic gastrointestinal biopsy samples and major resections, causing possible diagnostic conundrums for the young and not so young pathologists. The main aim of this review is the description of the most frequent oddities one can encounter as foreign bodies, in gastrointestinal pathology, on the basis of the current literature and personal experience. The types of encountered substances are divided into four principal categories: parasites (helminths such as Enterobius vermicularis, Strongyloides, Schistosoma, and Anisakis, and protozoa such as Entamoeba, Giardia and some intestinal coccidia); drugs and pharmaceutical fillers (found as deposits and as bystanders, innocent or not); seeds (possibly confused with worms) and plant remnants; pollutants (secondary to post-resection or post-biopsy contamination of the sample). An ample library of images is provided in order to consent easy referencing for diagnostic routine.
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Affiliation(s)
- Federica Grillo
- IRCCS Ospedale Policlinico San Martino, Anatomic Pathology Unit, Genova, Italy
- Anatomic Pathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genova, Largo Rosanna Benzi 10, 16132, Genova, Italy
| | - Michela Campora
- Anatomic Pathology Section, S. Chiara Hospital, Trento, Italy
| | - Luca Carlin
- Anatomic Pathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genova, Largo Rosanna Benzi 10, 16132, Genova, Italy
| | - Laura Cornara
- Department for the Earth, Environment and Life Sciences (DiSTAV), University of Genoa, Genoa, Italy
| | - Paola Parente
- Pathology Unit, Fondazione IRCCS Ospedale Casa Sollievo Della Sofferenza, San Giovanni Rotondo, FG, Italy
| | - Alessandro Vanoli
- Anatomic Pathology Unit, Department of Molecular Medicine, University of Pavia, and IRCCS San Matteo Hospital, Pavia, Italy
| | - Andrea Remo
- Pathology Unit, Service Department, ULSS9 "Scaligera", Verona, Italy
| | - Paola Migliora
- Clinical Cytopathology Service and Pathology Institute of Locarno, Locarno, Switzerland
| | - Fiocca Roberto
- IRCCS Ospedale Policlinico San Martino, Anatomic Pathology Unit, Genova, Italy
- Anatomic Pathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genova, Largo Rosanna Benzi 10, 16132, Genova, Italy
| | - Matteo Fassan
- Surgical Pathology Unit, Department of Medicine (DIMED), University of Padua, Padua, Italy
- Veneto Institute of Oncology IOV - IRCCS, Padua, Italy
| | - Luca Mastracci
- IRCCS Ospedale Policlinico San Martino, Anatomic Pathology Unit, Genova, Italy.
- Anatomic Pathology, Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genova, Largo Rosanna Benzi 10, 16132, Genova, Italy.
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Current Status of Anisakiasis and Anisakis Larvae in Tokyo, Japan. Food Saf (Tokyo) 2022; 9:89-100. [PMID: 35004097 PMCID: PMC8691968 DOI: 10.14252/foodsafetyfscj.d-21-00004] [Citation(s) in RCA: 18] [Impact Index Per Article: 6.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/21/2021] [Accepted: 09/02/2021] [Indexed: 11/22/2022] Open
Abstract
Anisakiasis is a gastrointestinal disease caused by infection with anisakid nematodes. Anisakis larvae have been listed as distinct food poisoning agents in the manual of Food Poisoning Statistics, Japan since 2013. The reported numbers of food poisoning cases caused by Anisakis larvae are gradually increasing. A total of 94.0% of the causative larvae species were identified as Anisakis simplex sensu stricto (A. simplex), and 4.4% were identified as Anisakis pegreffii, among human-isolated anisakid nematodes examined in Tokyo Metropolitan Institute of Public Health, Japan from 2011 to 2018. Anisakis species infecting fishes in Japanese waters differ depending on their habitat and depth. A. simplex mainly infects fishes in the Pacific side of Japan, and A. pegreffii mainly infects fishes in the East China Sea and Sea of Japan sides. Regarding the causative foods of anisakiasis, cases by ingestion of mackerel (Scomber spp.) have been the most common in Japan, and cases caused by eating “marinated mackerel” accounted for 32.8% of the total in Tokyo from 2011 to 2017. However, the number of reports of food poisoning caused by skipjack tuna (Katsuwonus pelamis) was highest in May 2018 in Japan. A parasitological surveys of Anisakis third-stage larvae in skipjack tuna in Japanese waters were conducted in 2018 and 2019, and it was confirmed that more A. simplex infections of skipjack tuna may have occurred in 2018 than usual due to the meandering flow of the Black Current. Moreover, a portion of A. simplex larvae migrated from visceral organs to the ventral muscle in live skipjack tuna before capture, suggesting that an extensive cold chain after capture cannot prevent anisakiasis. In fish species that were reported to be high frequency of causative food of anisakiasis, it is necessary to freeze or at least remove the ventral muscle.
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12
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Metazoan endoparasite fauna and feeding ecology of commercial fishes from Java, Indonesia. Parasitol Res 2022; 121:551-562. [PMID: 34993639 PMCID: PMC8800894 DOI: 10.1007/s00436-021-07377-4] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/15/2021] [Accepted: 11/08/2021] [Indexed: 11/10/2022]
Abstract
Despite being an important component of the marine ecosystem and posing health risks to human seafood consumers, fish parasites in Indonesia have yet to be adequately described. Here, we analyzed the diet and metazoan parasite fauna of seven commercial fish species (Alectis indica, Carangoides chrysophrys, Johnius borneensis, Mene maculata, Trichiurus lepturus, Upeneus asymmetricus, U. moluccensis) landed in Java, Indonesia. We isolated 11 endoparasite species, established 22 new host and 14 new locality records, and extended parasitological records of A. indica by 24%, C. chrysophrys by 25%, J. borneensis by 40%, M. maculata by 44%, U. asymmetricus by 100%, and U. moluccensis by 17%. We genetically identified the trematode Stephanostomum cf. uku (of Bray et al. 2005) from Alecta indica for the first time in Indonesia and provided the sequence of its 28S marker. Stomach content analysis revealed seven different prey items, and the examined fish species were grouped into four feeding categories, which differed significantly in their respective endoparasite fauna. All but two examined fish species hosted potentially zoonotic nematodes, which reveal a risk for parasite-borne diseases in Indonesian food fishes and call for more consequent monitoring with regard to seafood safety in this region. With this study, we were able to establish an association between the feeding ecology and the endoparasite fauna of marine fishes which will help to better understand the transmission pathways of (potentially zoonotic) parasites in food fishes in tropical waters.
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Cascarano MC, Stavrakidis-Zachou O, Mladineo I, Thompson KD, Papandroulakis N, Katharios P. Mediterranean Aquaculture in a Changing Climate: Temperature Effects on Pathogens and Diseases of Three Farmed Fish Species. Pathogens 2021; 10:1205. [PMID: 34578236 PMCID: PMC8466566 DOI: 10.3390/pathogens10091205] [Citation(s) in RCA: 38] [Impact Index Per Article: 9.5] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/09/2021] [Revised: 09/03/2021] [Accepted: 09/13/2021] [Indexed: 02/07/2023] Open
Abstract
Climate change is expected to have a drastic effect on aquaculture worldwide. As we move forward with the agenda to increase and diversify aquaculture production, rising temperatures will have a progressively relevant impact on fish farming, linked to a multitude of issues associated with fish welfare. Temperature affects the physiology of both fish and pathogens, and has the potential to lead to significant increases in disease outbreaks within aquaculture systems, resulting in severe financial impacts. Significant shifts in future temperature regimes are projected for the Mediterranean Sea. We therefore aim to review and discuss the existing knowledge relating to disease outbreaks in the context of climate change in Mediterranean finfish aquaculture. The objective is to describe the effects of temperature on the physiology of both fish and pathogens, and moreover to list and discuss the principal diseases of the three main fish species farmed in the Mediterranean, namely gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax), and meagre (Argyrosomus regius). We will attempt to link the pathology of each disease to a specific temperature range, while discussing potential future disease threats associated with the available climate change trends for the Mediterranean Sea.
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Affiliation(s)
- Maria Chiara Cascarano
- Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, 71500 Heraklion, Greece; (M.C.C.); (O.S.-Z.); (N.P.)
- Department of Biology, University of Crete, 71003 Heraklion, Greece
| | - Orestis Stavrakidis-Zachou
- Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, 71500 Heraklion, Greece; (M.C.C.); (O.S.-Z.); (N.P.)
- Department of Biology, University of Crete, 71003 Heraklion, Greece
| | - Ivona Mladineo
- Biology Center of Czech Academy of Sciences, Laboratory of Functional Helminthology, Institute of Parasitology, 370 05 Ceske Budejovice, Czech Republic;
| | - Kim D. Thompson
- Vaccines and Diagnostics, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, UK;
| | - Nikos Papandroulakis
- Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, 71500 Heraklion, Greece; (M.C.C.); (O.S.-Z.); (N.P.)
| | - Pantelis Katharios
- Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, 71500 Heraklion, Greece; (M.C.C.); (O.S.-Z.); (N.P.)
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Biology, Epidemiology, Clinical Features, Diagnosis, and Treatment of Selected Fish-borne Parasitic Zoonoses. THE YALE JOURNAL OF BIOLOGY AND MEDICINE 2021; 94:297-309. [PMID: 34211350 PMCID: PMC8223542] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 12/03/2022]
Abstract
Fish-borne parasites have been part of the global landscape of food-borne zoonotic diseases for many decades and are often endemic in certain regions of the world. The past 20 years or so have seen the expansion of the range of fish-borne parasitic zoonoses to new geographic regions leading to a substantial public health burden. In this article, we summarize current knowledge about the biology, epidemiology, clinical characteristics, diagnosis, treatment and control of selected fish-borne helminthic diseases caused by parasitic roundworm (Anisakis), tapeworm (Dibothriocephalus), and fluke (Metagonimus). Humans acquire infection via consumption of raw or improperly cooked fish or fish products. The burden from these diseases is caused by morbidity rather than mortality. Infected patients may present with mild to severe gastrointestinal (eg, abdominal pain, diarrhea, and indigestion) or allergic manifestations. Patients are often admitted to the hospital or clinic with acute symptoms and no prior health problems and no travel history. Diagnosis is often established based on the detection of the diagnostic parasite stages (eg, eggs or tapeworm segments) in the patient's feces. Sometimes imaging is required to exclude other causes and avoid unnecessary surgery. Dibothriocephalus and Metagonimus are mainly treated with praziquantel. Extraction of adult Dibothriocephalus or Anisakis larvae from the bowel ensures complete elimination of the parasites and prevents a relapse of infection. The development and implementation of more efficient food safety and public health strategies to reduce the burden of zoonotic diseases attributable to fish-borne parasites is highly desirable.
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Arcos SC, Lira F, Robertson L, González MR, Carballeda-Sangiao N, Sánchez-Alonso I, Zamorano L, Careche M, Jiménez-Ruíz Y, Ramos R, Llorens C, González-Muñoz M, Oliver A, Martínez JL, Navas A. Metagenomics Analysis Reveals an Extraordinary Inner Bacterial Diversity in Anisakids (Nematoda: Anisakidae) L3 Larvae. Microorganisms 2021; 9:1088. [PMID: 34069371 PMCID: PMC8158776 DOI: 10.3390/microorganisms9051088] [Citation(s) in RCA: 4] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/29/2021] [Revised: 05/05/2021] [Accepted: 05/10/2021] [Indexed: 11/28/2022] Open
Abstract
L3 larvae of anisakid nematodes are an important problem for the fisheries industry and pose a potential risk for human health by acting as infectious agents causing allergies and as potential vectors of pathogens and microrganisms. In spite of the close bacteria-nematode relationship very little is known of the anisakids microbiota. Fresh fish could be contaminated by bacteria vectored in the cuticle or in the intestine of anisakids when the L3 larvae migrate through the muscles. As a consequence, the bacterial inoculum will be spread, with potential effects on the quality of the fish, and possible clinical effects cannot be discarded. A total of 2,689,113 16S rRNA gene sequences from a total of 113 L3 individuals obtained from fish captured along the FAO 27 fishing area were studied. Bacteria were taxonomically characterized through 1803 representative operational taxonomic units (OTUs) sequences. Fourteen phyla, 31 classes, 52 orders, 129 families and 187 genera were unambiguously identified. We have found as part of microbiome an average of 123 OTUs per L3 individual. Diversity indices (Shannon and Simpson) indicate an extraordinary diversity of bacteria at an OTU level. There are clusters of anisakids individuals (samples) defined by the associated bacteria which, however, are not significantly related to fish hosts or anisakid taxa. This suggests that association or relationship among bacteria in anisakids, exists without the influence of fishes or nematodes. The lack of relationships with hosts of anisakids taxa has to be expressed by the association among bacterial OTUs or other taxonomical levels which range from OTUs to the phylum level. There are significant biological structural associations of microbiota in anisakid nematodes which manifest in clusters of bacteria ranging from phylum to genus level, which could also be an indicator of fish contamination or the geographic zone of fish capture. Actinobacteria, Aquificae, Firmicutes, and Proteobacteria are the phyla whose abundance value discriminate for defining such structures.
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Affiliation(s)
- Susana C. Arcos
- Museo Nacional de Ciencias Naturales, Dpto Biodiversidad y Biología Evolutiva, CSIC, 28006 Madrid, Spain; (S.C.A.); (L.R.); (M.R.G.); (Y.J.-R.)
| | - Felipe Lira
- Centro Nacional de Biotecnología, Departamento de Biotecnología Microbiana, CSIC, 28049 Madrid, Spain; (F.L.); (J.L.M.)
| | - Lee Robertson
- Museo Nacional de Ciencias Naturales, Dpto Biodiversidad y Biología Evolutiva, CSIC, 28006 Madrid, Spain; (S.C.A.); (L.R.); (M.R.G.); (Y.J.-R.)
- Departamento de Protección Vegetal, INIA, 28040 Madrid, Spain
| | - María Rosa González
- Museo Nacional de Ciencias Naturales, Dpto Biodiversidad y Biología Evolutiva, CSIC, 28006 Madrid, Spain; (S.C.A.); (L.R.); (M.R.G.); (Y.J.-R.)
| | | | - Isabel Sánchez-Alonso
- Instituto de Ciencia y Tecnología de Alimentos y Nutrición, CSIC, 28040 Madrid, Spain; (I.S.-A.); (M.C.)
| | - Laura Zamorano
- Servicio de Microbiología y Unidad de Investigación, Hospital Son Espases, (IdISPa), 07120 Palma de Mallorca, Spain; (L.Z.); (A.O.)
| | - Mercedes Careche
- Instituto de Ciencia y Tecnología de Alimentos y Nutrición, CSIC, 28040 Madrid, Spain; (I.S.-A.); (M.C.)
| | - Yolanda Jiménez-Ruíz
- Museo Nacional de Ciencias Naturales, Dpto Biodiversidad y Biología Evolutiva, CSIC, 28006 Madrid, Spain; (S.C.A.); (L.R.); (M.R.G.); (Y.J.-R.)
| | - Ricardo Ramos
- Unidad de Genómica, “Scientific Park of Madrid”, Campus de Cantoblanco, 28049 Madrid, Spain;
| | - Carlos Llorens
- Biotechvana, “Scientific Park”, University of Valencia, 46980 Valencia, Spain;
| | - Miguel González-Muñoz
- Servicio de Immunología, Hospital Universitario La Paz, 28046 Madrid, Spain; (N.C.-S.); (M.G.-M.)
| | - Antonio Oliver
- Servicio de Microbiología y Unidad de Investigación, Hospital Son Espases, (IdISPa), 07120 Palma de Mallorca, Spain; (L.Z.); (A.O.)
| | - José L. Martínez
- Centro Nacional de Biotecnología, Departamento de Biotecnología Microbiana, CSIC, 28049 Madrid, Spain; (F.L.); (J.L.M.)
| | - Alfonso Navas
- Museo Nacional de Ciencias Naturales, Dpto Biodiversidad y Biología Evolutiva, CSIC, 28006 Madrid, Spain; (S.C.A.); (L.R.); (M.R.G.); (Y.J.-R.)
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HELMINTH FAUNA OF ICE SEALS IN THE ALASKAN BERING AND CHUKCHI SEAS, 2006-15. J Wildl Dis 2021; 56:863-872. [PMID: 32502360 DOI: 10.7589/2019-09-228] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 09/06/2019] [Accepted: 03/28/2020] [Indexed: 11/20/2022]
Abstract
Climate warming may affect the distribution of helminth parasites, allowing endemic species to increase in prevalence and new species to appear. We analyzed tissues from 141 ice-associated seals collected in the Alaskan (US) Bering and Chukchi seas during 2006-15 for internal helminth parasites and compared results with past studies. Specimens were collected from: ringed seals (Pusa hispida), bearded seals (Erignathus barbatus), spotted seals (Phoca largha), and ribbon seals (Histriophoca fasciata). Helminths were present in 94% (133/141) of the seals sampled. Nematodes were most prevalent in bearded (97%, 72/74) and spotted seals (93%, 13/14). Cestodes were most prevalent in bearded seals (82%, 61/74) and absent in ribbon seals, trematodes were only found in bearded (64%, 47/74) and ringed (5%, 2/44) seals, and acanthocephalans were mostly found in ringed (61%, 27/44) and spotted (64%, 9/14) seals. Although no helminths were new to the Bering-Chukchi Seas region, this study found a previously unreported host record for the lungworm Parafilaroides (Filaroides) gymnurus in a ribbon seal. We also found the lungworm Otostrongylus circumlitus in a ribbon seal and P. (F.) gymnurus in bearded seals, representing location records previously unreported from the Bering-Chukchi Seas region (although they have been reported from the Sea of Okhotsk). We found the cestode genus Pyramicocephalus in bearded seals (3%, 2/74) at a lower prevalence than was reported previously for Pyramicocephalus phocarum (44-100%) in the Bering-Chukchi Seas region. We found no species of the acanthocephalan genus Bolbosoma, although the genus was previously identified in ringed, spotted, and ribbon seals. This study yielded no new helminths and no increases in the prevalence of endemic parasites in these seal species.
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Correlation of NHR-48 Transcriptional Modulator Expression with Selected CYP Genes’ Expression during Thiabendazole Treatment of Anisakis simplex (s.l.)?—An In Vitro Study. Pathogens 2020; 9:pathogens9121030. [PMID: 33316888 PMCID: PMC7764245 DOI: 10.3390/pathogens9121030] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 10/29/2020] [Revised: 11/28/2020] [Accepted: 12/07/2020] [Indexed: 11/18/2022] Open
Abstract
Anisakis simplex (s.l.) is a complex of three sibling (biological) species of parasitic nematodes of marine mammals, including A. berlandi, A. pegreffii and A. simplex (s.s.). It is characterized by a complex life cycle in which humans can become accidental hosts by consuming dishes made of raw or undercooked fish containing L3 larvae, which in many regions of the world is related to the national or regional culinary tradition. This has spurred scientific efforts to develop new methods for treating the disease, called anisakiasis, and to neutralize invasive L3. Thiabendazole (TBZ) is a wide-spectrum anthelminthic with a higher efficacy than albendazole, a drug whose long-term use induces resistance in many parasitic species. Cytochromes P450 participate in TBZ metabolism, and the expression of their genes is controlled by nuclear hormone receptors (NHR). This study aimed to examine the effects of TBZ on the above-described pathway in invasive larvae of A. simplex (s.l.). The efficacy of TBZ against A. simplex (s.l.) larvae was observed for the first time. Larvae were cultured in vitro for 72 h in a medium containing TBZ at five concentrations from 0.5 to 1.5 mM. However, the survival curves did not significantly differ from each other. This means that all of the concentrations of TBZ had a similar effect on the A. simplex (s.l.) L3 larvae during in vitro culture. Nevertheless, TBZ modified the expression of nhr-48, cyp13a3 and cyp1a1 genes in the L3 of A. simplex (s.l.).
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Ogata N, Tagishi H, Tsuji M. Inhibition of Acetylcholinesterase by Wood Creosote and Simple Phenolic Compounds. Chem Pharm Bull (Tokyo) 2020; 68:1193-1200. [PMID: 33268651 DOI: 10.1248/cpb.c20-00583] [Citation(s) in RCA: 2] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/22/2022]
Abstract
Anisakiasis is common in countries where raw or incompletely cooked marine fish are consumed. Currently, effective therapeutic methods to treat anisakiasis are unavailable. A recent study found that wood creosote inactivates the movement of Anisakis species. Essential oil of Origanum compactum containing carvacrol and thymol, which are similar to the constituents of wood creosote, was reported to inactivate Anisakis by inhibiting its acetylcholinesterase. We examined whether wood creosote can also inhibit acetylcholinesterase. We examined the effect of components of wood creosote using the same experimental method. A computer simulation experiment (molecular docking) was also performed. Here, we demonstrate that wood creosote inactivated acetylcholinesterase in a dose-dependent manner with an IC50 of 0.25 mg/mL. Components of wood creosote were also tested individually: 5-methylguaiacol, p-cresol, guaiacol, o-cresol, 2,4-dimethylphenol, m-cresol, phenol and 4-methylguaiacol inactivated the enzyme with an IC50 of 14.0, 5.6, 17.0, 6.3, 3.9, 10.0, 15.2 and 27.2 mM, respectively. The mechanism of acetylcholinesterase inactivation was analyzed using a computer-based molecular docking simulation, which employed a three-dimensional structure of acetylcholinesterase and above phenolic compounds as docking ligands. The simulation indicated that the phenolic compounds bind to the active site of the enzyme, thereby competitively blocking entry of the substrate acetylcholine. These findings suggest that the mechanism for the inactivation of Anisakis movement by wood creosote is due to inhibition of acetylcholinesterase needed for motor neuron activity.
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Affiliation(s)
- Norio Ogata
- R&D Department, Taiko Pharmaceutical Co., Ltd
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19
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The Selection of Reliable Reference Genes for RT-qPCR Analysis of Anisakis simplex Sensu Stricto Gene Expression from Different Developmental Stages. Acta Parasitol 2020; 65:837-842. [PMID: 32488545 PMCID: PMC7679296 DOI: 10.2478/s11686-020-00220-3] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/14/2020] [Accepted: 04/23/2020] [Indexed: 01/28/2023]
Abstract
BACKGROUND Anisakis simplex s. s. is a parasitic nematode with a complex life cycle in which humans can become accidental hosts by consuming raw or not fully cooked fish containing L3 larvae. The growing popularity of raw fish dishes has contributed to an increase in the incidence of anisakiasis, which has spurred scientific efforts to develop new methods for diagnosing and treating the disease and also to investigate the gene expression at different developmental stages of this parasite. The identification of reference genes suitable for the normalization of RT-qPCR data has not been studied with respect to A. simplex s. s. METHODS In the present study, eight candidate reference genes were analyzed in A. simplex s. s. at two different developmental stages: L3 and L4. The expression stability of these genes was assessed by geNorm and NormFinder softwares. RESULTS In general, our results identified translation elongation factor 1α (ef-1α) and peptidyl-prolyl isomerase 12 (ppi12) as the most stable genes in L3 and L4 developmental stages of A. simplex s. s. Validation of the selected reference genes was performed by profiling the expression of the nuclear hormone receptor gene (nhr 48) in different developmental stages. CONCLUSIONS This first analysis selecting suitable reference genes for RT-qPCR in A. simplex s. s. will facilitate future functional analyses and deep mining of genetic resources in this parasitic nematode.
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Ascaridoid nematode larvae in wild gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax) caught in the Tyrrhenian Sea (Western Mediterranean Sea): a contribute towards the parasitological risk assessment on two commercially important fish species. Food Control 2020. [DOI: 10.1016/j.foodcont.2020.107377] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/17/2022]
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Affiliation(s)
- Sho Fukui
- Immuno-Rheumatology Center, St. Luke's International Hospital, Tokyo, Japan
| | - Takahiro Matsuo
- Department of Infectious Diseases, St. Luke's International Hospital, Tokyo, Japan
| | - Nobuyoshi Mori
- Department of Infectious Diseases, St. Luke's International Hospital, Tokyo, Japan
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Hope W, Smith-Chakmakova F, Snyder J. Case of anisakiasis presenting as an Amyand hernia. BMJ Case Rep 2020; 13:13/7/e234822. [PMID: 32641316 DOI: 10.1136/bcr-2020-234822] [Citation(s) in RCA: 0] [Impact Index Per Article: 0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/04/2022] Open
Abstract
This is a case of a 31-year-old male patient who presented with signs and symptoms of an incarcerated inguinal hernia. The patient's preoperative imaging showed a tubular structure in the inguinal canal and given the patient's history at presentation, there was a concern for herniation of the appendix, known as an Amyand hernia. On laparoscopy, there was no evidence of appendiceal involvement and a standard open inguinal hernia was completed. On the final pathology of the hernia sac, roundworms were identified with Y-shaped lateral cords suggesting infection by Anisakis spp. On a further interview with the patient, he revealed that he had recently travelled to Alaska and had consumed raw salmon on a fishing trip. This case demonstrates the importance of a thorough social and travel history. One should also have a low threshold to broaden the differential diagnosis when medical work-up deviates from the standard course.
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Affiliation(s)
- William Hope
- Department of General Surgery, Virginia Commonwealth University Health System, Richmond, Virginia, USA
| | - Faye Smith-Chakmakova
- Department of Pathology, WellSpan Good Samaritan Hospital, Lebanon, Pennsylvania, USA
| | - Justin Snyder
- Department of Surgery, WellSpan Good Samaritan Hospital, Lebanon, Pennsylvania, USA
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A preliminary report on the awareness and knowledge of seafood-borne parasitic diseases among medical doctors in Australia. Parasitol Int 2020; 74:101993. [DOI: 10.1016/j.parint.2019.101993] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 08/02/2019] [Revised: 09/10/2019] [Accepted: 09/11/2019] [Indexed: 11/21/2022]
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Cammilleri G, Ferrantelli V, Pulvirenti A, Drago C, Stampone G, Del Rocio Quintero Macias G, Drago S, Arcoleo G, Costa A, Geraci F, Di Bella C. Validation of a Commercial Loop-Mediated Isothermal Amplification (LAMP) Assay for the Rapid Detection of Anisakis spp. DNA in Processed Fish Products. Foods 2020; 9:E92. [PMID: 31963107 PMCID: PMC7022600 DOI: 10.3390/foods9010092] [Citation(s) in RCA: 16] [Impact Index Per Article: 3.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/27/2019] [Revised: 01/07/2020] [Accepted: 01/09/2020] [Indexed: 12/11/2022] Open
Abstract
Parasites belonging to the Anisakis genera are organisms of interest for human health because they are responsible for the Anisakiasis zoonosis, caused by the ingestion of raw or undercooked fish. Furthermore, several authors have reported this parasite to be a relevant inducer of acute or chronic allergic diseases. In this work, a rapid commercial system based on Loop-Mediated Isothermal Amplification (LAMP) was optimised and validated for the sensitive and rapid detection of Anisakis spp. DNA in processed fish products. The specificity and sensitivity of the LAMP assay for processed fish samples experimentally infected with Anisakis spp. larvae and DNA were determined. The LAMP system proposed in this study was able to give positive amplification for all the processed fish samples artificially contaminated with Anisakis spp., giving sensitivity values equal to 100%. Specificity tests provided no amplification for the Contracaecum, Pseudoterranova, or Hysterothylacium genera and uninfected samples. The limit of detection (LOD) of the LAMP assay proposed was 102 times lower than the real-time PCR method compared. To the best of our knowledge, this is the first report regarding the application of the LAMP assay for the detection of Anisakis spp. in processed fish products. The results obtained indicate that the LAMP assay validated in this work could be a reliable, easy-to-use, and convenient tool for the rapid detection of Anisakis DNA in fish product inspection.
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Affiliation(s)
- Gaetano Cammilleri
- Istituto Zooprofilattico Sperimentale della Sicilia, via Gino Marinuzzi 3, 90129 Palermo, Italy; (V.F.); (A.C.); (F.G.); (C.D.B.)
- Dipartimento di Scienze della Vita, Università degli studi di Modena e Reggio Emilia, Via Università 4, 41121 Modena, Italy;
| | - Vincenzo Ferrantelli
- Istituto Zooprofilattico Sperimentale della Sicilia, via Gino Marinuzzi 3, 90129 Palermo, Italy; (V.F.); (A.C.); (F.G.); (C.D.B.)
| | - Andrea Pulvirenti
- Dipartimento di Scienze della Vita, Università degli studi di Modena e Reggio Emilia, Via Università 4, 41121 Modena, Italy;
| | - Chiara Drago
- Enbiotech s.r.l. Via Aquileia 34, 90144 Palermo, Italy; (C.D.); (G.S.); (G.D.R.Q.M.); (S.D.); (G.A.)
| | - Giuseppe Stampone
- Enbiotech s.r.l. Via Aquileia 34, 90144 Palermo, Italy; (C.D.); (G.S.); (G.D.R.Q.M.); (S.D.); (G.A.)
| | | | - Sandro Drago
- Enbiotech s.r.l. Via Aquileia 34, 90144 Palermo, Italy; (C.D.); (G.S.); (G.D.R.Q.M.); (S.D.); (G.A.)
| | - Giuseppe Arcoleo
- Enbiotech s.r.l. Via Aquileia 34, 90144 Palermo, Italy; (C.D.); (G.S.); (G.D.R.Q.M.); (S.D.); (G.A.)
| | - Antonella Costa
- Istituto Zooprofilattico Sperimentale della Sicilia, via Gino Marinuzzi 3, 90129 Palermo, Italy; (V.F.); (A.C.); (F.G.); (C.D.B.)
| | - Francesco Geraci
- Istituto Zooprofilattico Sperimentale della Sicilia, via Gino Marinuzzi 3, 90129 Palermo, Italy; (V.F.); (A.C.); (F.G.); (C.D.B.)
| | - Calogero Di Bella
- Istituto Zooprofilattico Sperimentale della Sicilia, via Gino Marinuzzi 3, 90129 Palermo, Italy; (V.F.); (A.C.); (F.G.); (C.D.B.)
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Betson M, Alonte AJI, Ancog RC, Aquino AMO, Belizario VY, Bordado AMD, Clark J, Corales MCG, Dacuma MG, Divina BP, Dixon MA, Gourley SA, Jimenez JRD, Jones BP, Manalo SMP, Prada JM, van Vliet AHM, Whatley KCL, Paller VGV. Zoonotic transmission of intestinal helminths in southeast Asia: Implications for control and elimination. ADVANCES IN PARASITOLOGY 2020; 108:47-131. [PMID: 32291086 DOI: 10.1016/bs.apar.2020.01.036] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.8] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Grants] [Track Full Text] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
Intestinal helminths are extremely widespread and highly prevalent infections of humans, particularly in rural and poor urban areas of low and middle-income countries. These parasites have chronic and often insidious effects on human health and child development including abdominal problems, anaemia, stunting and wasting. Certain animals play a fundamental role in the transmission of many intestinal helminths to humans. However, the contribution of zoonotic transmission to the overall burden of human intestinal helminth infection and the relative importance of different animal reservoirs remains incomplete. Moreover, control programmes and transmission models for intestinal helminths often do not consider the role of zoonotic reservoirs of infection. Such reservoirs will become increasingly important as control is scaled up and there is a move towards interruption and even elimination of parasite transmission. With a focus on southeast Asia, and the Philippines in particular, this review summarises the major zoonotic intestinal helminths, risk factors for infection and highlights knowledge gaps related to their epidemiology and transmission. Various methodologies are discussed, including parasite genomics, mathematical modelling and socio-economic analysis, that could be employed to improve understanding of intestinal helminth spread, reservoir attribution and the burden associated with infection, as well as assess effectiveness of interventions. For sustainable control and ultimately elimination of intestinal helminths, there is a need to move beyond scheduled mass deworming and to consider animal and environmental reservoirs. A One Health approach to control of intestinal helminths is proposed, integrating interventions targeting humans, animals and the environment, including improved access to water, hygiene and sanitation. This will require coordination and collaboration across different sectors to achieve best health outcomes for all.
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Affiliation(s)
- Martha Betson
- University of Surrey, Guildford, Surrey, United Kingdom.
| | | | - Rico C Ancog
- University of the Philippines Los Baños, Laguna, Philippines
| | | | | | | | - Jessica Clark
- University of Surrey, Guildford, Surrey, United Kingdom
| | | | | | - Billy P Divina
- University of the Philippines Los Baños, Laguna, Philippines
| | | | | | | | - Ben P Jones
- University of Surrey, Guildford, Surrey, United Kingdom
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Allison C, Baumann M. A Case Report of Adhesional Small Bowel Obstruction Caused by Extraintestinal Anisakiasis. J Emerg Med 2019; 57:e175-e179. [DOI: 10.1016/j.jemermed.2019.08.038] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/11/2019] [Revised: 08/22/2019] [Accepted: 08/25/2019] [Indexed: 11/29/2022]
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Łopieńska-Biernat E, Paukszto Ł, Jastrzębski JP, Myszczyński K, Polak I, Stryiński R. Genome-wide analysis of Anisakis simplex sensu lato: the role of carbohydrate metabolism genes in the parasite's development. Int J Parasitol 2019; 49:933-943. [PMID: 31560928 DOI: 10.1016/j.ijpara.2019.06.006] [Citation(s) in RCA: 12] [Impact Index Per Article: 2.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/15/2019] [Revised: 06/19/2019] [Accepted: 06/24/2019] [Indexed: 01/08/2023]
Abstract
Anisakis simplex sensu lato is a parasitic nematode which can cause gastric symptoms and/or allergic reactions in humans who consume raw and undercooked fish. Anisakiasis poses a growing health problem around the globe because it causes non-specific symptoms and is difficult to diagnose. This genome-wide study was undertaken to expand our knowledge of A. simplex s.l. at the molecular level and provide novel data for biological and biotechnological research into the analyzed species and related nematodes. A draft genome assembly of the L3 stage of A. simplex s.l. was analyzed in detail, and changes in the expression of carbohydrate metabolism genes during the parasite's life cycle were determined. To our knowledge, this is the first genome to be described for a parasitic nematode of the family Anisakidae to date. We identified genes involved in parasite-specific pathways, including carbohydrates metabolism, apoptosis and chemo signaling. A total of 7607 coding genes were predicted. The genome of A. simplex s.l. is highly similar to genomes of other parasitic nematodes. In particular, we described a valuable repository of genes encoding proteins of trehalose and glycogen metabolism, and we developed the most comprehensive data set relating to the conversion of both saccharides which play important roles during the parasite's life cycle in a host environment. We also confirmed that trehalose is synthesized at the expense of glycogen. Trehalose anabolism and glycogen catabolism were the predominant processes in stages L4 and L5, which could confirm our and other authors' previous reports that trehalose is synthesized at the expense of glycogen. The A. simplex s.l. genome provides essential data for post-genomic research into the biology of gastrointestinal and allergic anisakiasis in humans and the biology of other important parasitic helminths.
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Affiliation(s)
- Elżbieta Łopieńska-Biernat
- Department of Biochemistry, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland.
| | - Łukasz Paukszto
- Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Jan Paweł Jastrzębski
- Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Kamil Myszczyński
- Department of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727 Olsztyn, Poland
| | - Iwona Polak
- Department of Biochemistry, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Robert Stryiński
- Department of Biochemistry, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
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Joo SK, Kim JW, Kim BG, Kim W, Lee JK, Lee KL. Clinical and Endoscopic Features of Colonic Anisakiasis in Korea. THE KOREAN JOURNAL OF PARASITOLOGY 2019; 57:411-416. [PMID: 31533408 PMCID: PMC6753305 DOI: 10.3347/kjp.2019.57.4.411] [Citation(s) in RCA: 4] [Impact Index Per Article: 0.7] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 05/23/2019] [Accepted: 08/12/2019] [Indexed: 11/23/2022]
Abstract
To analyze the clinical and endoscopic features of colonic anisakiasis. A retrospective chart review of 20 patients with colonic anisakiasis, who were diagnosed by colonoscopy at 8 hospitals between January 2002 and December 2011, was performed. Patients’ mean age was 53.6±10.74 years. Seventy percent patients were men. Acute abdominal pain was a common symptom that mostly developed within 48 hr after the ingestion of raw fish, and which lasted for 1–28 days. Sixty percent patients had ingested raw fish before the diagnosis of colonic anisakiasis and 40% patients were incidentally found to have colonic anisakiasis during the screening colonoscopies. Leukocytosis and eosinophilia were each found in 20% of the patients. In all patients who underwent colonoscopy, the worms were removed with biopsy forceps, except in 1 case, and a definite diagnosis of anisakiasis was made. In some cases of colonic anisakiasis, colonoscopy may be helpful in the diagnosis and treatment to avoid surgical intervention.
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Affiliation(s)
- Sae Kyung Joo
- Seoul National University Seoul Metropolitan Government Boramae Medical Center - Internal Medicine, Seoul 07061, Korea
| | - Ji Won Kim
- Seoul National University Seoul Metropolitan Government Boramae Medical Center - Internal Medicine, Seoul 07061, Korea
| | - Byeong Gwan Kim
- Seoul National University Seoul Metropolitan Government Boramae Medical Center - Internal Medicine, Seoul 07061, Korea
| | - Won Kim
- Seoul National University Seoul Metropolitan Government Boramae Medical Center - Internal Medicine, Seoul 07061, Korea
| | - Jae Kyung Lee
- Seoul National University Seoul Metropolitan Government Boramae Medical Center - Internal Medicine, Seoul 07061, Korea
| | - Kook Lae Lee
- Seoul National University Seoul Metropolitan Government Boramae Medical Center - Internal Medicine, Seoul 07061, Korea
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Guardone L, Nucera D, Rosellini N, Tinacci L, Acutis P, Guidi A, Armani A. Occurrence, distribution and viability of Anisakis spp. larvae in various kind of marketed herring products in Italy. Food Control 2019. [DOI: 10.1016/j.foodcont.2019.02.030] [Citation(s) in RCA: 5] [Impact Index Per Article: 0.8] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/14/2022]
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Hrabar J, Trumbić Ž, Bočina I, Bušelić I, Vrbatović A, Mladineo I. Interplay between proinflammatory cytokines, miRNA, and tissue lesions in Anisakis-infected Sprague-Dawley rats. PLoS Negl Trop Dis 2019; 13:e0007397. [PMID: 31091271 PMCID: PMC6538193 DOI: 10.1371/journal.pntd.0007397] [Citation(s) in RCA: 13] [Impact Index Per Article: 2.2] [Reference Citation Analysis] [Abstract] [MESH Headings] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/28/2019] [Revised: 05/28/2019] [Accepted: 04/16/2019] [Indexed: 12/29/2022] Open
Abstract
Background Anisakiasis is an emerging public health problem, caused by Anisakis spp. nematode larvae. Anisakiasis presents as variable and unspecific gastrointestinal and/or allergic clinical symptoms, which accounts for the high rate of misdiagnosed cases. Methodology/Principal findings The aim of this study was to characterize the early cellular (6–72 h p.i.) and molecular (6 h p.i.) immune response and general underlying regulatory mechanism in Anisakis infected rats. Each Sprague-Dawley rat was infected with 10 Anisakis spp. larvae by gastric intubation. Tissues with visible lesions were processed for: i) classic histopathology (HE), immunofluorescence (CD3, iNOS, S100A8/A9), and transmission electron microscopy (TEM); ii) target genes (Il1b, Il6, Il18, Ccl3, Icam1, Mmp9) and microRNA (Rat Immunopathology MIRN-104ZF plate, Quiagen) expression analysis; and iii) global DNA methylation. Histopathology revealed that Anisakis larval migration caused moderate to extensive hemorrhages in submucosal and epimysial/perimysial connective tissue. In stomach and muscle, moderate to abundant mixed inflammatory infiltrate was present, dominated by neutrophils and macrophages, while only mild infiltration was seen in intestine. Lesions were characterized by the presence of CD3+, iNOS+, and S100A8/A9+ cells. The greatest number of iNOS+ and S100A8/A9+ cells was seen in muscle. Il6, Il1b, and Ccl3 showed particularly strong expression in stomach and visceral adipose tissues, but the order of expression differed between tissues. In total, three miRNAs were differentially expressed, two in stomach (miRNA-451 and miRNA-223) and two in intestine (miRNA-451 and miRNA-672). No changes in global DNA methylation were observed in infected tissues relative to controls. Conclusions/Significance Anisakis infection induces strong immune responses in infected rats with marked induction of specific proinflammatory cytokines and miRNA expression. Deciphering the functional role of these cytokines and miRNAs will help in understanding the anisakiasis pathology and controversies surrounding Anisakis infection in humans. Anisakiasis is a zoonotic disease (infection transmitted between animals and humans) contracted by consumption of raw or undercooked seafood contaminated with Anisakis spp. nematode larvae. Anisakiasis usually presents with variable and unspecific gastrointestinal and/or allergic symptoms, which accounts for the high rate of misdiagnosed cases. Due to changes in dietary habits, such as eating raw or undercooked seafood, anisakiasis is considered an emerging public health problem. Despite the increase in number of reported cases worldwide, mechanisms of immune response to this unspecific human pathogen are poorly known. We have shown that in experimentally infected rats, Anisakis larvae cause severe hemorrhages and necrotic changes of affected tissues in the early phase of infections. Neutrophils and macrophages were abundantly present in tissue lesions, while eosinophils, hallmark of helminth infections, were scarcely present. We have also demonstrated particularly strong expression of several inflammatory genes. Moreover, we give for the first-time insight into putative regulatory mechanism mediated via a distinct class of RNA molecules. Our study may provide new opportunities for better understanding of cellular and molecular response to Anisakis spp., aiming at development of more specific therapeutics and alleviation of pathologies associated with Anisakis spp. infection.
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Affiliation(s)
- Jerko Hrabar
- Laboratory of Aquaculture, Institute of Oceanography and Fisheries, Split, Croatia
- * E-mail:
| | - Željka Trumbić
- Department of Marine Studies, University of Split, Split, Croatia
| | - Ivana Bočina
- Faculty of Science, University of Split, Split, Croatia
| | - Ivana Bušelić
- Laboratory of Aquaculture, Institute of Oceanography and Fisheries, Split, Croatia
| | - Anamarija Vrbatović
- Laboratory of Aquaculture, Institute of Oceanography and Fisheries, Split, Croatia
| | - Ivona Mladineo
- Laboratory of Aquaculture, Institute of Oceanography and Fisheries, Split, Croatia
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Łopieńska-Biernat E, Paukszto Ł, Jastrzębski JP, Makowczenko K, Stryiński R. Genes expression and in silico studies of functions of trehalases, a highly dispersed Anisakis simplex s. l. specific gene family. Int J Biol Macromol 2019; 129:957-964. [DOI: 10.1016/j.ijbiomac.2019.02.042] [Citation(s) in RCA: 6] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 12/17/2018] [Revised: 02/07/2019] [Accepted: 02/07/2019] [Indexed: 10/27/2022]
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Chronic epigastric pain associated with gastric anisakidosis. IDCases 2019; 15:e00515. [PMID: 30911466 PMCID: PMC6416658 DOI: 10.1016/j.idcr.2019.e00515] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/26/2019] [Revised: 03/01/2019] [Accepted: 03/01/2019] [Indexed: 11/20/2022] Open
Abstract
Anisakidosis is an acute and, less commonly, chronic gastrointestinal tract disease caused by the ingestion of nematode larvae contained within raw or undercooked seafood. We describe a case of chronic gastric anisakidosis diagnosed by endoscopic resection of submucosal nodules in a patient with a multi-year history of epigastric abdominal pain. This case illustrates the importance of maintaining a high index of suspicion for this diagnosis, even in patients with a remote history of seafood consumption or a chronic presentation of abdominal pain.
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Łopieńska-Biernat E, Stryiński R, Dmitryjuk M, Wasilewska B. Infective larvae of Anisakis simplex (Nematoda) accumulate trehalose and glycogen in response to starvation and temperature stress. Biol Open 2019; 8:bio040014. [PMID: 30824422 PMCID: PMC6451339 DOI: 10.1242/bio.040014] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [Grants] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 11/06/2018] [Accepted: 02/20/2019] [Indexed: 12/13/2022] Open
Abstract
Anisakis simplex L3 larvae infect fish and other seafood species such as squid or octopi; therefore, humans consuming raw or undercooked fish may become accidental hosts for this parasite. These larvae are induced to enter hypometabolism by cold temperatures. It is assumed that sugars (in particular trehalose and glycogen) are instrumental for survival under environmental stress conditions. To elucidate the mechanisms of environmental stress response in A. simplex, we observed the effects of starvation and temperature on trehalose and glycogen content, the activity of enzymes metabolizing those sugars, and the relative expression of genes of trehalose and glycogen metabolic pathways. The L3 of A. simplex synthesize trehalose both in low (0°C) and high temperatures (45°C). The highest content of glycogen was observed at 45°C at 36 h of incubation. On the second day of incubation, tissue content of trehalose depended on the activity of the enzymes: TPS was more active at 45°C, and TPP was more active at 0°C. The changes in TPP activity were consistent with the transcript level changes of the TPP gene, and the trehalose level, while glycogen synthesis correlates with the expression of glycogen synthase gene at 45°C; this suggests that the synthesis of trehalose is more essential. These results show that trehalose plays a key role in providing energy during the thermotolerance and starvation processes through the molecular and biochemical regulation of trehalose and glycogen metabolism.
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Affiliation(s)
- Elżbieta Łopieńska-Biernat
- Department of Biochemistry, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Robert Stryiński
- Department of Biochemistry, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Małgorzata Dmitryjuk
- Department of Biochemistry, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Barbara Wasilewska
- Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Plac Łódzki 3, 10-727 Olsztyn, Poland
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Hara Y, Uruma T, Morishima Y, Hirai Y. 'Tingling throat syndrome' as asymptomatic anisakiasis following conveyor belt sushi consumption in Tokyo. Int J Infect Dis 2019; 82:102-103. [PMID: 30904673 DOI: 10.1016/j.ijid.2019.03.019] [Citation(s) in RCA: 1] [Impact Index Per Article: 0.2] [Reference Citation Analysis] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 02/21/2019] [Revised: 03/12/2019] [Accepted: 03/14/2019] [Indexed: 11/15/2022] Open
Affiliation(s)
- Yutaro Hara
- Department of Pediatrics, Tokyo Medical University Hachioji Medical Center, Japan
| | - Tomonori Uruma
- Department of Infectious Diseases, Tokyo Medical University Hachioji Medical Center, Japan
| | - Yasuyuki Morishima
- Department of Parasitology, National Institute of Infectious Diseases, Japan
| | - Yuji Hirai
- Department of Infectious Diseases, Tokyo Medical University Hachioji Medical Center, Japan.
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Cammilleri G, Pulvirenti A, Costa A, Graci S, Collura R, Buscemi MD, Sciortino S, Vitale Badaco V, Vazzana M, Brunone M, Vella A, Di Bella C, Ferrantelli V. Seasonal trend of Anisakidae infestation in South Mediterranean bluefish. Nat Prod Res 2019; 34:158-161. [PMID: 30784309 DOI: 10.1080/14786419.2019.1573232] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.5] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/27/2022]
Abstract
A total of 1104 fish samples from markets of Sicily were analysed for the detection and species identification of Anisakidae nematodes. The preliminary analysis of the fish samples showed the presence of 2459 larvae. All the fish species revealed different prevalence of infestation, with a maximum of 100% for Lepidopus caudatus and a minimum of 4.5% in Sardina pilchardus. The 80% of the larvae examined by PCR-RFLP analysis belonged to Anisakis pegreffii species. The seasonal infestation trend of Anisakis was evaluated in all the fish sample examined. The results of the seasonal infestation trend showed a marked connection with the ecological aspects of the fish species examined. As far as we know, this work report for the first time important ecological aspects of Lepidopus caudatus specimens of South Mediterranean. This work could be useful to plan a seasonal fishing strategy aimed at reducing the health risks related to Anisakis.
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Affiliation(s)
- Gaetano Cammilleri
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
| | - Andrea Pulvirenti
- Dipartimento Scienze della Vita, Università degli studi di Modena e Reggio Emilia, Modena, Italy
| | - Antonella Costa
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
| | - Stefania Graci
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
| | - Rosaria Collura
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
| | | | - Sonia Sciortino
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
| | | | - Mirella Vazzana
- Dipartimento STEBICEF, Università degli studi di Palermo, Palermo, Italy
| | - Mariagrazia Brunone
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
| | - Antonio Vella
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
| | - Calogero Di Bella
- Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Palermo, Italy
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Murata Y, Ando K, Usui M, Sugiyama H, Hayashi A, Tanemura A, Kato H, Kuriyama N, Kishiwada M, Mizuno S, Sakurai H, Isaji S. A case of hepatic anisakiasis caused by Pseudoterranova decipiens mimicking metastatic liver cancer. BMC Infect Dis 2018; 18:619. [PMID: 30514220 PMCID: PMC6280358 DOI: 10.1186/s12879-018-3540-8] [Citation(s) in RCA: 11] [Impact Index Per Article: 1.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/28/2018] [Accepted: 11/22/2018] [Indexed: 12/17/2022] Open
Abstract
BACKGROUND Anisakid nematodes (Anisakis spp. or Pseudoterranova spp.) usually infect gastric or intestinal walls, while they rarely infect in extra-gastrointestinal sites of human body. Generally, Anisakis spp. larvae are highly infected in fish intermediate hosts, whereas Pseudoterranova spp. larvae are very rarely infected. To the best of our knowledge, there have been no reports which have documented cases of hepatic anisakiasis caused by Pseudoterranova spp. This report describes the first documented case of hepatic anisakiasis due to infection with Pseudoterranova decipiens and clinical features of the hepatic anisakiasis through literature review. CASE PRESENTATION The case was a 28-year-old man with prior history of malignancy who was found to have a hepatic mass mimicking metastatic liver tumor. A new low density area of 20 mm in diameter in liver segment 7 was found on follow-up CT. With suspicious diagnosis of metastatic liver cancer, laparoscopic partial hepatectomy was performed. A pathological examination revealed no evidence of malignancy, but showed necrotic granuloma with eosinophil infiltration and the presence of a larva with Y-shaped lateral cords, which are specific to anisakid larvae. The type of larva was identified as Pseudoterranova decipiens sensu lato using PCR of DNA purified from a fixed granuloma embedded in paraffin. CONCLUSION The present report is the first to discuss the case of a patient with hepatic anisakiasis caused by Pseudoterranova decipiens. Hepatic anisakiasis is a potential differential diagnosis for hepatic tumors and genetic identification with the PCR method was reliable for obtaining final diagnosis even when the larvae body in the resected specimen collapses with time.
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Affiliation(s)
- Yasuhiro Murata
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Katsuhiko Ando
- Department of Medical Zoology, Mie University Graduate School of Medicine, Tsu, Japan
| | - Masanobu Usui
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Hiromu Sugiyama
- Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan
| | - Akinobu Hayashi
- Department of Pathology, Mie University Graduate School of Medicine, Tsu, Japan
| | - Akihiro Tanemura
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Hiroyuki Kato
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Naohisa Kuriyama
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Masashi Kishiwada
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Shugo Mizuno
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Hiroyuki Sakurai
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
| | - Shuji Isaji
- Department of Hepatobiliary Pancreatic and Transplant surgery, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507 Japan
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Cole R, Viney M. The population genetics of parasitic nematodes of wild animals. Parasit Vectors 2018; 11:590. [PMID: 30424774 PMCID: PMC6234597 DOI: 10.1186/s13071-018-3137-5] [Citation(s) in RCA: 30] [Impact Index Per Article: 4.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/06/2018] [Accepted: 10/08/2018] [Indexed: 12/17/2022] Open
Abstract
Parasitic nematodes are highly diverse and common, infecting virtually all animal species, and the importance of their roles in natural ecosystems is increasingly becoming apparent. How genes flow within and among populations of these parasites - their population genetics - has profound implications for the epidemiology of host infection and disease, and for the response of parasite populations to selection pressures. The population genetics of nematode parasites of wild animals may have consequences for host conservation, or influence the risk of zoonotic disease. Host movement has long been recognised as an important determinant of parasitic nematode population genetic structure, and recent research has also highlighted the importance of nematode life histories, environmental conditions, and other aspects of host ecology. Commonly, factors influencing parasitic nematode population genetics have been studied in isolation, such that an integrated view of the drivers of population genetic structure of parasitic nematodes is still lacking. Here, we seek to provide a comprehensive, broad, and integrative picture of these factors in parasitic nematodes of wild animals that will be a useful resource for investigators studying non-model parasitic nematodes in natural ecosystems. Increasingly, new methods of analysing the population genetics of nematodes are becoming available, and we consider the opportunities that these afford in resolving hitherto inaccessible questions of the population genetics of these important animals.
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Affiliation(s)
- Rebecca Cole
- School of Biological Sciences, University of Bristol, Bristol, BS8 1TQ, UK.
| | - Mark Viney
- School of Biological Sciences, University of Bristol, Bristol, BS8 1TQ, UK
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Walden H, Grijalva CJ, Paez-Rosas D, Hernandez J. Intestinal parasites in Galapagos sea lions (Zalophus wollebaeki) Sivertsen, 1953 on San Cristóbal Island, Galápagos, Ecuador. J Parasitol 2018; 104:718-721. [PMID: 30141720 DOI: 10.1645/17-187] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 11/10/2022] Open
Abstract
Knowledge regarding endoparasites of Galapagos sea lions, Zalophus wollebaeki Sivertsen, 1953, is limited to 1 report. Herein, we examined feces extracted from the lower gastro-intestinal tract of 15 Galapagos sea lions (GSL) plus 14 fecal mounds nearby GSL at 4 locations on San Cristobal Island, Galapagos beginning in May continuing through June 2016. Using standard fecal flotation and sedimentation techniques, lungworm larvae, suggestive of Parafilaroides and Otostrongylus sp., eggs of pseudophyllidea cestodes and anisakid nematodes, and coccidian oocysts were collected from study samples. This is the first report of potential lungworm larvae, anisakids, pseudophyllidea cestodes and coccidian parasites in GSL.
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Affiliation(s)
- Heather Walden
- * University of Florida College of Veterinary Medicine Research Assistant Professor Infectious Diseases and Pathology University of Florida College of Veterinary Medicine
- ‡ University of Florida
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Guardone L, Armani A, Nucera D, Costanzo F, Mattiucci S, Bruschi F. Human anisakiasis in Italy: a retrospective epidemiological study over two decades. Parasite 2018; 25:41. [PMID: 30058531 PMCID: PMC6065268 DOI: 10.1051/parasite/2018034] [Citation(s) in RCA: 60] [Impact Index Per Article: 8.6] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Figures] [Journal Information] [Subscribe] [Scholar Register] [Received: 04/12/2018] [Accepted: 06/12/2018] [Indexed: 12/24/2022] Open
Abstract
A retrospective analysis on human anisakiasis in Italy since its first description in 1996 was performed by conducting a literature search. Inclusion criteria based on the presence of a larva and on parasite identification were applied. Epidemiological data and clinical features were analysed. Particular attention was paid to the source of infection. In total, 73 cases were included in the analysis, while 34 were excluded. Cases were reported from eight Italian regions, most frequently Abruzzo, Apulia and Latium. The parasite was detected by endoscopy (51.4%) or laparotomy (48.6%). The site of infection was intestinal (42.5%), gastric (43.8%), oesophageal (1.4%) or ectopic (12.3%). Most of the parasites (71.0%) were identified as Anisakis sp. or A. simplex (s.l.). However, when molecular methods were used (21 cases), A. pegreffii was always identified. In most of the patients (65.7%), the source of infection was raw or undercooked anchovies, followed by "anchovies or sardines" (15.1%), generic "raw seafood" (15.1%), and sardines (1.4%). In only 2 cases (2.7%), the source was not available. This is the first systematic analysis of Italian cases of anisakiasis. The main conclusions derived from the results are: i) attention should be given to the history, in particular when raw marinated anchovies, proven to be the main source of human anisakiasis in Italy, are consumed; ii) in order to assess correct epidemiological data, a confirmed and specific etiological identification should always be sought.
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Affiliation(s)
- Lisa Guardone
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FishLab, Department of Veterinary Sciences, University of Pisa Via delle Piagge 2 56124
Pisa Italy
| | - Andrea Armani
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FishLab, Department of Veterinary Sciences, University of Pisa Via delle Piagge 2 56124
Pisa Italy
| | - Daniele Nucera
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Department of Agriculture, Forestry and Food Science, University of Turin Largo Braccini 2 10095
Grugliasco – Torino Italy
| | - Francesco Costanzo
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FishLab, Department of Veterinary Sciences, University of Pisa Via delle Piagge 2 56124
Pisa Italy
| | - Simonetta Mattiucci
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Department of Public Health and Infectious Diseases, Section of Parasitology, “Sapienza – University of Rome”, Laboratory affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti P.le Aldo Moro, 5 00185
Rome Italy
| | - Fabrizio Bruschi
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Department of Translational Research, N.T.M.S., School of Medicine, University of Pisa via Roma, 55 56126
Pisa Italy
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Abstract
This manuscript presents a review of infectious causes of gastritis aimed at the practicing anatomic pathologist. We shall highlight unique histologic findings and clinical attributes that will assist those analyzing endoscopically obtained mucosal biopsies of the stomach or resection specimens.
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Serrano-Moliner M, Morales-Suarez-Varela M, Valero MA. Epidemiology and management of foodborne nematodiasis in the European Union, systematic review 2000-2016. Pathog Glob Health 2018; 112:249-258. [PMID: 29957154 PMCID: PMC6225410 DOI: 10.1080/20477724.2018.1487663] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 12/13/2022] Open
Abstract
BACKGROUND Foodborne nematodiasis are caused by the ingestion of food contaminated by helminths. In Europe, these diseases are present in all countries. OBJECTIVES To review the available data on epidemiology and management of foodborne nematodiasis in the European Union, detect any trends and determine the possible causes of the observed changes. METHODS A review of available literature published between 2000 and 2016 was conducted. RESULTS Out of 1523 cases described in the literature, 1493 cases were autochthonous and 30 cases were imported. The detected parasites were Toxocara spp (34.7%), Ascaris lumbricoides (27.1%), Trichinella spp (21.9%), Anisakis spp (15.5%) and Angiostrongylus cantonensis (0.8%). CONCLUSIONS Foodborne nematodiasis remains a public health challenge for the European Union. Autochthonous cases of nematodiasis present the greatest health risk within the European Union. Foodborne nematodes due to lack of hygiene in food processing are diseases that can be avoided by increasing.
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Affiliation(s)
- Marta Serrano-Moliner
- Department of Cellular Biology and Parasitology, University of Valencia, Burjassot, Spain
| | - María Morales-Suarez-Varela
- Department of Preventive Medicine and Public Health, Food Science, Toxicology and Legal Medicine, University of Valencia, Burjassot, Spain
- CIBER of Epidemiology and Public Health (CIBERESP), Centro de Investigacion Biomedica en Red (CIBER), Madrid, Spain
| | - M. Adela Valero
- Department of Cellular Biology and Parasitology, University of Valencia, Burjassot, Spain
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Eiras JC, Pavanelli GC, Takemoto RM, Nawa Y. An Overview of Fish-borne Nematodiases among Returned Travelers for Recent 25 Years- Unexpected Diseases Sometimes Far Away from the Origin. THE KOREAN JOURNAL OF PARASITOLOGY 2018; 56:215-227. [PMID: 29996625 PMCID: PMC6046559 DOI: 10.3347/kjp.2018.56.3.215] [Citation(s) in RCA: 12] [Impact Index Per Article: 1.7] [Reference Citation Analysis] [Abstract] [Key Words] [MESH Headings] [Track Full Text] [Download PDF] [Subscribe] [Scholar Register] [Received: 01/25/2018] [Revised: 05/06/2018] [Accepted: 05/14/2018] [Indexed: 12/12/2022]
Abstract
Along with globalization of traveling and trading, fish-borne nematodiases seems to be increasing in number. However, apart from occasional and sporadic case reports or mini-reviews of particular diseases in particular countries, an overview of fish-borne nematodiasis among travelers have never been performed. In this review, we gathered fishborne nematodiasis among travelers for recent 25 years by an extensive global literature survey using appropriate keywords, e.g. travelers diseases, human infection, anisakiasis, gnathostomiasis, capillariasis, sushi, sashimi, ceviche, Gnathostoma, Pseudoterranova, Anisakis, Capillaria, etc., as well as various combinations of these key words. The Internet search engines PubMed, Medline, Google and Googler Scholar were used as much as possible, and the references of every paper were checked in order to identify useful and reliable publications. The results showed unexpectedly high incidence of gnathostomiasis and low incidence of anisakidosis. The different incidence values of the infection with several fish-borne zoonotic nematode species are discussed, as well as some epidemiological aspects of the infections. The difficulties of differential diagnosis in non-endemic countries are emphasized. It is concluded that travelers must avoid risky behaviors which can lead to infection and that physicians and health authorities must advice travelers on the risks of eating behaviors during travel.
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Affiliation(s)
- Jorge Costa Eiras
- Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, Edifício FC4, 4169-007 Porto, Portugal
- Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos, 4450-308 Matosinhos, Portugal
| | - Gilberto Cezar Pavanelli
- Unicesumar, Programa de pós-graduação em Promoção da Saúde, Bloco 7, Avenida Guerner Dias, 1610-Jardim Aclimação, Maringá-PR, 87050-900, Brasil
- Pesquisador do Instituto Cesumar de Ciência, Tecnologia e Inovação, Bloco 11-50 andar (44) 3027-6360 Ramal 1346, Brasil
| | | | - Yukifumi Nawa
- Tropical Diseases Research Center, Faculty of Medicine, Khon Kaen University, 123 Mitrapap Highway, Khon Kaen 40002, Thailand
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Fujikawa H, Kuwai T, Yamaguchi T, Miura R, Sumida Y, Takasago T, Miyasako Y, Nishimura T, Iio S, Imagawa H, Yamaguchi A, Kouno H, Kohno H. Gastric and enteric anisakiasis successfully treated with Gastrografin therapy: A case report. World J Gastrointest Endosc 2018; 10:69-73. [PMID: 29564036 PMCID: PMC5852334 DOI: 10.4253/wjge.v10.i3.69] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Journal Information] [Submit a Manuscript] [Subscribe] [Scholar Register] [Received: 01/09/2018] [Revised: 02/07/2018] [Accepted: 03/01/2018] [Indexed: 02/05/2023] Open
Abstract
We report a case of a 59-year-old woman who was diagnosed with gastric and small intestinal anisakiasis, which was successfully treated with endoscopic extraction and Gastrografin therapy. She was admitted to our hospital with epigastric pain and vomiting one day after eating raw fish. She exhibited tenderness in the epigastrium without obvious rebound tenderness or guarding. Computed tomography (CT) demonstrated segmental edema of the intestinal wall with proximal dilatation and a small number of ascites. Because enteric anisakiasis was suspected based on the patient’s history of recent raw fish consumption and abdominal CT, we performed gastroscopy and confirmed that nine Anisakis larvae were attached to the gastric mucosa. All of the Anisakis larvae were extracted via endoscopy, and the patient was diagnosed with gastric and enteric anisakiasis. Additionally, in the hospital, we performed ileography twice using Gastrografin, which led to shortened hospital stay. Based on the clinical results of this case, we suggest that Gastrografin therapy is a safe, convenient, and useful method to extract enteric Anisakis larvae.
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Affiliation(s)
- Hiroki Fujikawa
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Toshio Kuwai
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Toshiki Yamaguchi
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Ryoichi Miura
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Yuki Sumida
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Takeshi Takasago
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Yuki Miyasako
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Tomoyuki Nishimura
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Sumio Iio
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Hiroki Imagawa
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Atsushi Yamaguchi
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Hirotaka Kouno
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
| | - Hiroshi Kohno
- Department of Gastroenterology, National Hospital Organization, Kure Medical Center and Chugoku Cancer Center, Kure 737-0023, Japan
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Anisakis spp. larvae in different kinds of ready to eat products made of anchovies (Engraulis encrasicolus) sold in Italian supermarkets. Int J Food Microbiol 2018; 268:10-18. [PMID: 29306733 DOI: 10.1016/j.ijfoodmicro.2017.12.030] [Citation(s) in RCA: 20] [Impact Index Per Article: 2.9] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 07/15/2017] [Revised: 12/17/2017] [Accepted: 12/30/2017] [Indexed: 11/20/2022]
Abstract
In this study the occurrence of visible anisakid larvae in semi-preserved anchovy products sold on the Italian market was investigated. Totally, 107 ready to eat products (33 salted-ripened, 49 in oil and 25 marinated) were sampled. Each sample was digested, then the digested material was observed under natural and UV light. Parasites were counted, collected and microscopically identified to genus level. A representative subset was molecularly identified using the cox2 gene. At least one visible Anisakis sp. larva was found in 54.2% of the total 107 products analysed and totally 1283 dead larvae were collected. Anisakis sp. larvae were found in all the 33 salted products and 1139 (88.8%) larvae were collected, with a range of 1-105 parasites per product. Larval density per gram was 0.13. Anisakis sp. larvae were found in 49.0% of the products in oil and 143 (11.1%) larvae were isolated, with a range of 0-28 and a density of 0.03. Only 1 larva was found in the 25 marinated products (4.0%, density 0.00). A highly significant difference between all the product categories in respect of number of larvae per product, frequency of products contaminated by at least one larva and larval density per gram was found. Within the subset of larvae molecularly analysed (n=122), 92 (75.4%) were identified as A. pegreffii and 30 (24.6%) as A. simplex. This study showed that semi-preserved anchovy products heavily contaminated with Anisakis spp. larvae reach the market. Beyond the negligible risk for anisakidosis, the presence of dead visible parasites may cause immediate rejection in consumers. In addition, the potential risk related to allergic reactions in sensitized individuals needs to be further assessed. In order to avoid commercialization of obviously contaminated products, fresh anchovies' batches intended for the production of such products should be accurately selected by the processing industry applying inspection methods.
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Main Groups of Microorganisms of Relevance for Food Safety and Stability. INNOVATIVE TECHNOLOGIES FOR FOOD PRESERVATION 2018. [PMCID: PMC7150063 DOI: 10.1016/b978-0-12-811031-7.00003-0] [Citation(s) in RCA: 65] [Impact Index Per Article: 9.3] [Reference Citation Analysis] [Abstract] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Indexed: 12/12/2022]
Abstract
Microbiology is important to food safety, production, processing, preservation, and storage. Microbes such as bacteria, molds, and yeasts are employed for the foods production and food ingredients such as production of wine, beer, bakery, and dairy products. On the other hand, the growth and contamination of spoilage and pathogenic microorganisms is considered as one of the main causes to loss of foodstuff nowadays. Although technology, hygienic strategies, and traceability are important factors to prevent and delay microbial growth and contamination, food remains susceptible to spoilage and activity of pathogen microorganisms. Food loss by either spoilage or contaminated food affects food industry and consumers leading to economic losses and increased hospitalization costs. This chapter focuses on general aspects, characteristics, and importance of main microorganisms (bacteria, yeasts, molds, virus, and parasites) involved in food spoilage or contamination: known and recently discovered species; defects and alterations in foodstuff; most common food associated with each foodborne disease; resistance to thermal processing; occurrence in different countries; outbreaks; and associated symptoms.
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Ashida H, Igarashi T, Morikawa K, Motohashi K, Fukuda K, Tamai N. Distinguishing gastric anisakiasis from non-anisakiasis using unenhanced computed tomography. Abdom Radiol (NY) 2017. [PMID: 28643138 DOI: 10.1007/s00261-017-1214-4] [Citation(s) in RCA: 3] [Impact Index Per Article: 0.4] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Indexed: 10/19/2022]
Abstract
PURPOSE This study aimed to assess the diagnostic performance of unenhanced computed tomography (CT) for distinguishing gastric anisakiasis from non-anisakiasis gastric conditions and the reproducibility of CT findings. METHODS Fifty-six anisakiasis and 74 non-anisakiasis cases with gastric wall thickening on urgent observation using unenhanced CT were included. Using a κ analysis, two radiologists independently assessed the reproducibility of CT findings, including "circumferential gastric wall thickening," "gastric wall thickening extending more than two segments," "bulky and low-density gastric wall thickening," "increase in peri-gastric fat density," and "ascites." An anisakiasis diagnostic score (ADS) was developed for this study and was used to quantitatively evaluate the CT findings. An anisakiasis diagnostic prediction (ADP) with an appropriate cutoff value was used to further evaluate the ADS. Two radiologists reassessed the findings in consensus to determine the sensitivity, specificity, and accuracy of the CT findings, including the ADP and ADS area under the curve (AUC). RESULTS Considering reproducibility, a substantial agreement (0.6 < κ < 0.8) was achieved for all findings except "circumferential gastric wall thickening" (κ = 0.499), whereas for diagnostic performance, all findings except ascites were significantly more frequent among the anisakiasis cases. "Bulky and low-density gastric wall thickening" had the highest sensitivity (98%), whereas "gastric wall thickening extending more than two segments" had the highest specificity (80%). The ADP sensitivity, specificity, and accuracy were 91%, 84%, and 87%, respectively. The AUC was 0.902 (p < 0.05). CONCLUSIONS Unenhanced CT findings are useful for distinguishing anisakiasis from non-anisakiasis gastric conditions with sufficient reproducibility.
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Łopieńska-Biernat E, Molcan T, Paukszto Ł, Jastrzębski JP, Myszczyński K. Modelling studies determing the mode of action of anthelmintics inhibiting in vitro trehalose-6-phosphate phosphatase (TPP) of Anisakis simplex s.l. Exp Parasitol 2017; 184:46-56. [PMID: 29170085 DOI: 10.1016/j.exppara.2017.11.006] [Citation(s) in RCA: 10] [Impact Index Per Article: 1.3] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 06/22/2017] [Revised: 10/20/2017] [Accepted: 11/17/2017] [Indexed: 10/18/2022]
Abstract
The trehalose-6-phosphate phosphatase (TPP) enzyme is involved in the synthesis of trehalose, the main sugar in the energy metabolism of nematodes. TPP is a member of the HAD-like hydrolase superfamily and shows a robust and specific phosphatase activity for the substrate trehalose-6-phosphate. The presence of conserved active sites of TPP in closely related nematodes and its absence in humans makes it a promising target for antiparasitic drugs. In the present study, homology modeling, molecular docking and MD simulation techniques were used to explore the structure and dynamics of TPP. In the active site, a magnesium ion is stabilized by 3 coordinate bonds formed by D189, D191 and D400. The key amino acids involved in ligand binding by the enzyme are C198, Y201,T357, D191 and Y197. This study relied on docking to select potential inhibitors of TPP which were tested in vitro for sensitivity to anthelmintic drugs such as levamisole and ivermectin targeting Anisakis simplex. The higher toxicity of LEV than IVM was demonstrated after 96 h, 30% of larvae were motile in cultures with 100 μg/ml of LEV and 1000 μg/ml of IVM. We identified drug combination of LEV-IVM against in vitro A. simplex as agonistic effect (CI = 1.1). Levamisole appeared to be a more effective drug which inhibited enzyme activity after 48 h and expression of mRNA after 96 h at a concentration of 10 μg/ml. This preliminary study predicted the structure of TPP, and the results of an in vitro experiment involving A. simplex will contribute to the development of effective inhibitors with potential antiparasitic activity in the future.
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Affiliation(s)
- Elżbieta Łopieńska-Biernat
- Department of Biochemistry, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland.
| | - Tomasz Molcan
- Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
| | - Łukasz Paukszto
- Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Jan Paweł Jastrzębski
- Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland
| | - Kamil Myszczyński
- Department of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
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Gaglio G, Battaglia P, Costa A, Cavallaro M, Cammilleri G, Graci S, Buscemi MD, Ferrantelli V, Andaloro F, Marino F. Anisakis spp. larvae in three mesopelagic and bathypelagic fish species of the central Mediterranean Sea. Parasitol Int 2017; 67:23-28. [PMID: 28965943 DOI: 10.1016/j.parint.2017.09.010] [Citation(s) in RCA: 8] [Impact Index Per Article: 1.0] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Journal Information] [Subscribe] [Scholar Register] [Received: 01/30/2017] [Revised: 06/01/2017] [Accepted: 09/15/2017] [Indexed: 12/01/2022]
Abstract
In this work 437 fish samples of species belonging to the families Myctophidae (Electrona risso and Diaphus metopoclampus) and Phosichthyidae (Vinciguerria attenuata) were examined for the presence of Anisakidae larvae. The study was performed with fishes in the central Mediterranean Sea, particularly in the Strait of Sicily and in the Strait of Messina. The visual inspection and chloro-peptic analysis revealed the presence of nematode parasites with prevalence values between 2.9% in Electrona risso samples and 5.4% in Vinciguerria attenuata samples. A positive correlation was found between standard length (SL) and prevalence of infestation in D. metopoclampus samples (p<0.05). The larvae examined were morphologically ascribed, at genus level, to Anisakis morphotypes I and II and molecularly identified as Anisakis pegreffii, Anisakis ziphidarum and Anisakis physeteris, in 67%, 9% and 24% of the fish samples examined. Overall, A. pegreffii and A. ziphidarum larvae were isolated in 14 and 2 specimens of D. metopoclampus respectively, A. physeteris larvae were found in 3 E. risso and 2 V. attenuata. A positive correlation was found between standard length and prevalence of infestation in D. metopoclampus samples (p<0.05). First information is provided on the presence of Anisakis spp. larvae of the myctophid fish species E. risso, D. metopoclampus and V. attenuata from the Central Mediterranean. It is also confirmed the role of lanternfishes (Myctophidae) as paratenic hosts for Anisakis spp.
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Affiliation(s)
- G Gaglio
- Department of Veterinary Sciences, Centro di Ittiopatologia Sperimentale della Sicilia, Università degli studi di Messina, Polo Universitario Annunziata, Messina 98168, Italy
| | - P Battaglia
- ISPRA - Institute for Environmental Protection and Research, Laboratory of Ichthyology and Marine Ecology, via dei Mille 46, Milazzo, Messina 98057, Italy
| | - A Costa
- National Reference Centre for Anisakiasis, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", via G. Marinuzzi, Palermo 90129, Italy.
| | - M Cavallaro
- Department of Veterinary Sciences, Centro di Ittiopatologia Sperimentale della Sicilia, Università degli studi di Messina, Polo Universitario Annunziata, Messina 98168, Italy
| | - G Cammilleri
- National Reference Centre for Anisakiasis, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", via G. Marinuzzi, Palermo 90129, Italy
| | - S Graci
- National Reference Centre for Anisakiasis, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", via G. Marinuzzi, Palermo 90129, Italy
| | - M D Buscemi
- Department of Veterinary Sciences, Centro di Ittiopatologia Sperimentale della Sicilia, Università degli studi di Messina, Polo Universitario Annunziata, Messina 98168, Italy; National Reference Centre for Anisakiasis, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", via G. Marinuzzi, Palermo 90129, Italy
| | - V Ferrantelli
- National Reference Centre for Anisakiasis, Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", via G. Marinuzzi, Palermo 90129, Italy
| | - F Andaloro
- ISPRA - Institute for Environmental Protection and Research, Laboratory of Ichthyology and Marine Ecology, via dei Mille 46, Milazzo, Messina 98057, Italy
| | - F Marino
- Department of Veterinary Sciences, Centro di Ittiopatologia Sperimentale della Sicilia, Università degli studi di Messina, Polo Universitario Annunziata, Messina 98168, Italy
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Nieuwenhuizen NE. Anisakis - immunology of a foodborne parasitosis. Parasite Immunol 2017; 38:548-57. [PMID: 27428817 DOI: 10.1111/pim.12349] [Citation(s) in RCA: 63] [Impact Index Per Article: 7.9] [Reference Citation Analysis] [Abstract] [Key Words] [Journal Information] [Subscribe] [Scholar Register] [Received: 03/14/2016] [Accepted: 07/13/2016] [Indexed: 01/28/2023]
Abstract
Anisakis species are marine nematodes which can cause zoonotic infection in humans if consumed in raw, pickled or undercooked fish and seafood. Infection with Anisakis is associated with abdominal pain, nausea and diarrhoea and can lead to massive infiltration of eosinophils and formation of granulomas in the gastrointestinal tract if the larvae are not removed. Re-infection leads to systemic allergic reactions such as urticarial or anaphylaxis in some individuals, making Anisakis an important source of hidden allergens in seafood. This review summarizes the immunopathology associated with Anisakis infection. Anisakiasis and gastroallergic reactions can be prevented by consuming only fish that has been frozen to -20°C to the core for at least 24 hours before preparation. Sensitization to Anisakis proteins can also occur, primarily due to occupational exposure to infested fish, and can lead to dermatitis, rhinoconjunctivitis or asthma. In this case, exposure to fish should be avoided.
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Anisakidae nematodes isolated from the flathead grey mullet fish (Mugil cephalus) of Buenaventura, Colombia. INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE 2017; 6:265-270. [PMID: 28913166 PMCID: PMC5587874 DOI: 10.1016/j.ijppaw.2017.08.001] [Citation(s) in RCA: 9] [Impact Index Per Article: 1.1] [Reference Citation Analysis] [Abstract] [Key Words] [Track Full Text] [Download PDF] [Figures] [Subscribe] [Scholar Register] [Received: 06/09/2017] [Revised: 07/27/2017] [Accepted: 08/02/2017] [Indexed: 11/22/2022]
Abstract
Anisakiasis is a parasitic infection caused by larval stages of nematodes of the genus Anisakis, Pseudoterranova and Contracaecum, of the Anisakidae family. The lifecycle of these nematodes develops in aquatic organisms and their final hosts are marine mammals. However, humans can act as accidental hosts and become infected with infective stage larvae (L3) by consuming raw or undercooked fish or shellfish carrying the parasite. Of this group of parasites, the genus Anisakis is the most studied: its presence in humans is associated with non-specific gastrointestinal symptoms or allergic responses that can trigger anaphylactic shock. The lack of studies in anisakiasis and Anisakis in Colombia has resulted in this infection being little-known by medical practitioners and therefore potentially underreported. The objective of this study was to identify anisakid nematodes in the flathead grey mullet fish (Mugil cephalus), caught by artisanal fishing methods and commercialized in Buenaventura. Morphological identification was carried out by classical taxonomy complemented by microscopy study using the histochemical technique Hematoxylin-Eosin. Nematodes of the genus Anisakis were found in the host M. cephalus. The Prevalence of Anisakis larvae in flathead grey mullet fish was 33%. The findings confirm the presence of Anisakis sp. in fish for human consumption in the Colombian Pacific region, a justification for further investigation into a possible emerging disease in this country.
The research confirmed the presence of anisakids in the fish M. cephalus in Colombia. First report such anisakids in fish from Colombia's Pacific coast. Is confirmed the presence of the genus Anisakis in the country. Anisakis spp. is associated with allergic reactions and gastro-allergies in humans. The presence the anisakids suggests a possible emerging zoonotic disease in Colombia.
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