Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver

Table 1 Composition of the preservation solutions ViaSpan^® and BG35

	ViaSpan®	BG35
Impermeants (mmol/L)
Lactobionate	100
Gluconate		100
Raffinose	30
Buffers (mmol/L)
KH2PO4	25	2.5
BES		50
Substrates (mmol/L)
Allopurinol	1	1
Glutathione	3	3
Adenosine	5	5
Glycine		15
MgSO4	5	5
Colloids (g/L)
HES	50
PEG 35000		40
pH	7.40	7.40
Osm (mOsm/kg water)	320 ± 4	339 ± 4

Dexamethasone 16 mg/L, insulin 40 UI/L and penicillin G 200000 UI/L were added to ViaSpan^® before use. Streptomycin 0.25 mg/mL and penicillin G 10 UI/mL were added to BG35 before use. All the solutions were bubbled with 100 % N2 for 45 min at 0 °C before use. BES: N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid; HES: Hydroxyethyl starch; PEG: Polyethyleneglycol.

Table 2 Time course evolution of minibioreactor functional parameters during 120 min of perfusion

Perfusion time	(Osm)B/(Osm)BC	Hto (%)	Hemolysis (%)	(Glucose)B/(Glucose)BC	(NH4+)B/(NH4+)BC	QNH4+ (μmol)
0 min	0.94 ± 0.02	47 ± 3	0.27 ± 0.09	0.09 ± 0.04	52.8 ± 4.0	36.3 ± 1.6
60 min	1.00 ± 0.02	44 ± 5	0.59 ± 0.10	0.77 ± 0.07	1.1 ± 0.1	36.1 ± 1.6
120 min	1.00 ± 0.01	45 ± 3	0.79 ± 0.12	0.90 ± 0.06	1.2 ± 0.3	36.1 ± 1.5

_B: Blood; _BC: Biological compartment.