Homogeneous phenomenon of the graft when using different genotype characteristic of recipients/donors in living donor liver transplantation

Table 1 The proportional changes of G and A alleles of the CYP3A5*3 in the pyrosequencing with restriction fragment length polymorphism of peripheral blood mononuclear cells of the recipients and donors, and the liver graft biopsy samples after living donor liver transplantation

CYP3A5*3intron36986A > GA (wide type)Ssp I1	PS	Recipient	Donor	Graft(liver biopsy)		P value
						R vs G	D vs G
A > A/G, n = 10	A	86.23 ± 7.67	24.78 ± 3.90	n = 35	35.57 ± 7.52	< 0.0001	>0.05
	G	13.77 ± 7.67	75.23 ± 3.90		64.43 ± 7.52
A/G > A, n = 3	A	21.97 ± 1.32	73.13 ± 19.2	n = 5	68.92 ± 11.75	< 0.001	>0.05
	G	78.03 ± 1.32	26.87 ± 19.2		31.08 ± 11.75
A/G > G, n = 12	A	21.73 ± 5.81	6.44 ± 3.38	n = 33	9.95 ± 5.68	< 0.0001	>0.05
	G	78.27 ± 5.81	93.56 ± 3.38		90.05 ± 5.68
G > A, n = 2	A	3.06 ± 0.27	94.08 ± 5.87	n = 10	47.29 ± 9.44	< 0.0001	< 0.0001
	G	96.94 ± 0.27	5.92 ± 5.87		52.71 ± 9.44
G > A/G, n = 13	A	4.55 ± 1.88	22.97 ± 3.84	n = 31	18.75 ± 6.43	< 0.0001	= 0.033
	G	95.45 ± 1.88	77.03 ± 3.84		81.25 ± 6.43
A > G, n = 1	A	84.58	2.26	n = 5	11.60 ± 5.26	-	-
	G	15.42	97.74		88.39 ± 5.26
Total, n = 41				n = 119

¹Restriction enzyme for polymerase chain reaction-restriction fragment length polymorphism. D: Donor; G: Graft; PS: Pyrosequencing; R: Recipient.

Table 2 The proportional changes of C and T alleles of the MDR1-3435 in the pyrosequencing with restriction fragment length polymorphism of peripheral blood mononuclear cells of the recipients and donors, and the liver graft biopsy sample after living donor liver transplantation

MDR1-3435Exon263435C>TC (wide type)Sau3A I1	PS	Recipient	Donor	Graft (liver biopsy)		P value
						R vs G	D vs G
C > C/T, n = 14	C	93.10 ± 2.71	46.38 ± 1.94	n = 40	56.97 ± 5.17	< 0.0001	< 0.0001
	T	6.90 ± 2.71	53.62 ± 1.94		43.03 ± 5.17
C/T > C, n = 16	C	46.36 ± 1.75	92.94 ± 2.15	n = 31	81.96 ± 5.42	< 0.0001	< 0.0001
	T	53.64 ± 1.75	7.06 ± 2.15		18.04 ± 5.42
C/T > T, n = 3	C	46.35 ± 2.77	2.88 ± 1.49	n = 5	12.38 ± 6.46	< 0.001	> 0.05
	T	53.65 ± 2.77	97.12 ± 1.49		87.62 ± 6.46
T > C/T, n = 7	C	2.33 ± 0.48	47.52 ± 1.00	n = 27	34.38 ± 6.21	< 0.0001	> 0.05
	T	97.67 ± 0.48	52.47 ± 1.00		65.62 ± 6.21
T > C, n = 1	C	4.19	94.19	n = 5	63.93 ± 5.25	-	-
	T	95.81	5.81		36.07 ± 5.25
Total, n = 41				n = 108

¹Restriction enzyme for polymerase chain reaction-restriction fragment length polymorphism. D: Donor; G: Graft; PS: Pyrosequencing; R: Recipient.

Table 3 The proportional changes of T and C alleles of the CYP3A4*18 in the pyrosequencing with restriction fragment length polymorphism of peripheral blood mononuclear cells of the recipients and donors, and the liver graft biopsy samples after living donor liver transplantation

CYP3A4*18Exon10878T>CT (wide type)Hpa II1	PS	Recipient	Donor	Graft (liver biopsy)		P value
						R vs G	D vs G
T/C > T, n = 2	T	58.59 ± 3.08	99.02 ± 1.39	n = 12	91.86 ± 4.21	< 0.0001	0.033
	C	41.42 ± 3.08	0.99 ± 1.39		8.14 ± 4.21
T > T/C, n = 1	T	100	55.92	n = 3	65.23 ± 3.31	-	-
	C	0	44.08		34.77 ± 3.31
Total, n = 3				n = 15

¹Restriction enzyme for polymerase chain reaction-restriction fragment length polymorphism. D: Donor; G: Graft; PS: Pyrosequencing; R: Recipient.