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World J Hepatol. Oct 27, 2013; 5(10): 558-567
Published online Oct 27, 2013. doi: 10.4254/wjh.v5.i10.558
Published online Oct 27, 2013. doi: 10.4254/wjh.v5.i10.558
Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH
Stephan Laggai, Yvette Simon, Theo Ranssweiler, Alexandra K Kiemer, Sonja M Kessler, Department of Pharmacy, Pharmaceutical Biology, Saarland University, Saarbruecken 66123, Germany
Author contributions: Laggai S, Kiemer AK and Kessler SM designed experiments, analysed data and wrote the manuscript; Kessler SM and Kiemer AK initiated and directed the study; Simon Y and Ranssweiler T designed experiments and participated in data acquisition; Laggai S, Kiemer AK and Kessler SM contributed to revising it critically for important intellectual content; Laggai S, Simon Y, Ranssweiler T, Kiemer AK and Kessler SM contributed to final approval of the version to be published.
Supported by The Graduiertenförderung of Saarland University (Laggai S), an EASL Dame Sheila Sherlock Fellowship (Kessler SM); and by the research committee of Saarland University (61-cl/Anschub2012)
Correspondence to: Alexandra K Kiemer, PhD, Professor of Pharmaceutical Biology, Department of Pharmacy, Pharmaceutical Biology, Saarland University, Campus C2.2, Saarbruecken, 66123, Germany. pharm.bio.kiemer@mx.uni-saarland.de
Telephone: +49-681-30257301 Fax: +49-681-30257302
Received: July 17, 2013
Revised: September 23, 2013
Accepted: October 11, 2013
Published online: October 27, 2013
Processing time: 100 Days and 8.6 Hours
Revised: September 23, 2013
Accepted: October 11, 2013
Published online: October 27, 2013
Processing time: 100 Days and 8.6 Hours
Core Tip
Core tip: We describe a new method to quantify lipid classes in steatosis/steatohepatitis having advantages over both histology and classical analytical methods. Since lipid classes exert differential pathophysiological actions our method should be of interest for all researchers dealing with mechanisms of steatosis and steatohepatitis. We employ our method to investigate the lipid profile in the steatotic p62 transgenic mouse model. p62 was originally identified as an autoantigen overexpressed in hepatocellular carcinoma patients, its expression correlates with poor prognosis, and it induces steatosis. The interesting lipid profile in p62 transgenic animals suggests that it might advance the step from steatosis towards steatohepatitis.