Copyright
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Hepatol. Sep 27, 2020; 12(9): 596-618
Published online Sep 27, 2020. doi: 10.4254/wjh.v12.i9.596
Published online Sep 27, 2020. doi: 10.4254/wjh.v12.i9.596
N-acetylcysteine and glycyrrhizin combination: Benefit outcome in a murine model of acetaminophen-induced liver failure
Charlotte Minsart, Arnaud Lemmers, Eric Quertinmont, Thierry Gustot, Laboratory of Experimental Gastroenterology, Erasme Hospital, Université Libre de Bruxelles, Brussels 1070, Belgium
Sandrine Rorive, Department of Pathology, Erasme Hospital, Université Libre de Bruxelles, Brussels 1070, Belgium
Sandrine Rorive, DIAPATH-Center for Microscopy and Molecular Imaging, Université Libre de Bruxelles, Gosselies 6041, Belgium
Arnaud Lemmers, Thierry Gustot, Department of Gastroenterology, Hepato Pancreatology and Digestive Oncology, Erasme Hospital, Université Libre de Bruxelles, Brussels 1070, Belgium
Thierry Gustot, Inserm Unité 1149, Centre de Recherche sur l’inflammation, Paris 75006, France
Thierry Gustot, UMR S_1149, Université Paris Diderot, Paris 75006, France
Author contributions: All authors contributed to the study conception and design; Material preparation, data collection and analysis were performed by Minsart C; Immunohistochemistry and histological analyses were carried out with the help of Rorive S; The first draft of the manuscript was written by Minsart C and Gustot T; all authors commented on previous versions of the manuscript, and all read and approved the final manuscript.
Supported by the Bourse du Conseil Médical de l’hôpital Erasme, Fonds E. et S. Jacobs and Novartis Grant; The CMMI is supported by the European Regional Development Fund and Wallonia .
Institutional review board statement: This study did not involve humans and thus was exempt.
Institutional animal care and use committee statement: All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals. Animal protocols were approved by the local Ethic Committee of the Université Libre de Bruxelles (License No. 1230406, Animalerie de la Faculté de Médecine du Campus Erasme, Brussels, Belgium; Protocol Nos. 488N and 734N).
Conflict-of-interest statement: The authors declare they have no conflicts of interest in relation to this paper.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Charlotte Minsart, PhD, Research Scientist, Laboratory of Experimental Gastroenterology, Erasme Hospital, Université Libre de Bruxelles, 808, Route de Lennik, Brussels 1070, Belgium. charlotte.minsart@erasme.ulb.ac.be
Received: November 28, 2019
Peer-review started: November 28, 2019
First decision: December 30, 2019
Revised: June 29, 2020
Accepted: August 25, 2020
Article in press: August 25, 2020
Published online: September 27, 2020
Processing time: 297 Days and 20 Hours
Peer-review started: November 28, 2019
First decision: December 30, 2019
Revised: June 29, 2020
Accepted: August 25, 2020
Article in press: August 25, 2020
Published online: September 27, 2020
Processing time: 297 Days and 20 Hours
Core Tip
Core Tip: Acetaminophen overdose is the most common cause of drug-induced liver failure in the developed countries. Substantial progress has been made in understanding the mechanism of hepatocellular injury, but N-acetylcysteine remains the only effective treatment despite its short therapeutic window. We present here our first results on the combination of N-acetylcysteine and glycyrrhizin in a murine model of acetaminophen-induced liver injury. Acetaminophen toxicity was induced by an intraperitoneal dose of 500 mg/kg. Hepatotoxicity was assessed by biochemical and histopathological analyses. Survival rates were also compared. Our results suggest, for the first time, that the combination of N-acetylcysteine and glycyrrhizin may be effective in preventing acetaminophen-induced liver injury in mice.