Basic Study
Copyright ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Hepatol. May 27, 2020; 12(5): 184-206
Published online May 27, 2020. doi: 10.4254/wjh.v12.i5.184
Pentadecapeptide BPC 157 resolves Pringle maneuver in rats, both ischemia and reperfusion
Marijan Kolovrat, Slaven Gojkovic, Ivan Krezic, Dominik Malekinusic, Borna Vrdoljak, Katarina Kasnik Kovac, Tamara Kralj, Domagoj Drmic, Ivan Barisic, Katarina Horvat Pavlov, Andreja Petrovic, Antonija Duzel, Mario Knezevic, Ivan Mirkovic, Antonio Kokot, Alenka Boban Blagaic, Sven Seiwerth, Predrag Sikiric
Marijan Kolovrat, Slaven Gojkovic, Ivan Krezic, Dominik Malekinusic, Borna Vrdoljak, Katarina Kasnik Kovac, Tamara Kralj, Domagoj Drmic, Ivan Barisic, Katarina Horvat Pavlov, Andreja Petrovic, Antonija Duzel, Mario Knezevic, Ivan Mirkovic, Antonio Kokot, Alenka Boban Blagaic, Sven Seiwerth, Predrag Sikiric, Departments of Pharmacology and Pathology, School of Medicine, University of Zagreb, Zagreb 10000, Croatia
Author contributions: Kolovrat M, Seiwerth S and Sikiric P designed the research; Kolovrat M, Gojkovic S, Krezic I, Malekinusic D, Vrdoljak B, Kasnik Kovac K, Drmic D, Barisic I, Horvat Pavlov K, Duzel A, Mirkovic I, Kokot A, Seiwerth S and Sikiric P performed the research; Seiwerth S and Sikiric P contributed reagents and analytic tools; Kolovrat M, Gojkovic S, Krezic I, Malekinusic D, Vrdoljak B, Kasnik Kovac K, Drmic D, Barisic I, Horvat Pavlov K, Duzel A, Mirkovic I, Kokot A, Seiwerth S and Sikiric P analyzed the data; Kolovrat M, Seiwerth S and Sikiric P wrote the paper.
Institutional animal care and use committee statement: This research was approved by local Ethic Committee (case number 380-59-10106-17-100/290) and by Directorate of Veterinary (UP/I-322-01/15-01/22).
Conflict-of-interest statement: All other authors have nothing to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Predrag Sikiric, MD, PhD, Professor, Departments of Pharmacology and Pathology, School of Medicine, University of Zagreb, POB 916, Salata 11, Zagreb 10000, Croatia. sikiric@mef.hr
Received: October 23, 2019
Peer-review started: October 23, 2019
First decision: November 22, 2019
Revised: March 25, 2020
Accepted: March 30, 2020
Article in press: March 30, 2020
Published online: May 27, 2020
Processing time: 216 Days and 20.4 Hours
Core Tip

Core tip: Recently, cytoprotective agent BPC 157 successfully resolved vessel occlusions in rats (ischemic colitis; deep vein thrombosis, superior anterior pancreaticoduodenal vein) through rapid collateral vessel recruitment to circumvent vessel occlusion. Medication BPC 157 [(10 µg, 10 ng/kg) regimens, administered as a single challenge] picked (1) ischemia, portal triad obstruction (PTO) period [at 5 min before (ip) or at 5 or 30 min of ligation time (as a bath to PTO)] or (2) reperfusion, post-PTO period [at 1 or 15 min (bath during surgery) or 24 h (ip) reperfusion time]. Gross, microscopy, malondialdehyde, serum enzymes, electrocardiogram, portal, caval and aortal pressure, thrombosis and venography assessments demonstrated that BPC 157 successfully attenuates ischemia-reperfusion injury of the liver and other organs. In particular, BPC 157 rapidly activates portocaval shunt, normalises arterial and disturbed blood pressure (portal and caval hypertension and aortal hypotension), counteracts formation of blood clots and cardiac rhythm changes and counteracts gastrointestinal mucosal lesions, as complications that follow the Pringle maneuver.