Copyright
©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Hepatol. Oct 27, 2018; 10(10): 719-730
Published online Oct 27, 2018. doi: 10.4254/wjh.v10.i10.719
Published online Oct 27, 2018. doi: 10.4254/wjh.v10.i10.719
Experimental bio-artificial liver: Importance of the architectural design on ammonia detoxification performance
María Dolores Pizarro, María Eugenia Mamprin, Lucas Damián Daurelio, Joaquín Valentín Rodriguez, María Gabriela Mediavilla, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rosario S2002 LRK, Argentina
María Dolores Pizarro, Lucas Damián Daurelio, Laboratorio de Investigaciones en Fisiología y Biología Molecular Vegetal (LIFiBVe), Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza 3080, Argentina
María Eugenia Mamprin, Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario S2002 LRK, Argentina
Joaquín Valentín Rodriguez, Centro Binacional de Criobiología Clínica y Aplicada (CAIC), Universidad Nacional de Rosario, Rosario S2011 BXN, Argentina
María Gabriela Mediavilla, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Consejo Nacional de Investigaciones Científicas y Tecnológicas, y Universidad Nacional de Rosario, Rosario S2002 LRK, Argentina
Author contributions: Pizarro MD performed the majority of experiments, analyzed data, and wrote the manuscript; Mamprin ME and Mediavilla MG designed the research, contributed new reagents, analyzed data, and wrote the manuscript; Daurelio LD designed and supervised the statistical analysis; Rodriguez JV developed the cylindrical bioreactor and metabolite mass balance equations, performed a critical revision, and contributed to the research and the redaction of this article; all the authors were involved in reviewing the literature for latest contributions in the field, writing, and editing the manuscript; Mamprin ME and Mediavilla MG have equally contributed to this work.
Supported by Universidad Nacional de Rosario (UNR) , BIO 272, Resol. C.S., No. 677/2013 ; Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), PICT-03-14492, BID 1728 OC/AR (Argentina) ; and a grant from Regione Autonoma Friuli-Venezia Giulia, Italy .
Institutional review board statement: The study was reviewed and approved by the National University of Rosario Institutional Review Board (Resol. C.S. No. 677/2013).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Faculty of Biochemical and Pharmaceutical Sciences-UNR (Resol. No. 139/2011).
Conflict-of-interest statement: No potential conflicts of interest relevant to this article were reported.
Open-Access: This article is an open-access article, which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: María Gabriela Mediavilla, PhD, Associate Researcher, Doctor, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Consejo Nacional de Investigaciones Científicas y Tecnológicas, y Universidad Nacional de Rosario, Suipacha 531, Rosario S2002 LRK, Argentina. mediavilla@ibr-conicet.gov.ar
Telephone: +54-341-4350661 Fax: +54-341-4390465
Received: May 22, 2018
Peer-review started: May 23, 2018
First decision: June 14, 2018
Revised: July 12, 2018
Accepted: August 6, 2018
Article in press: August 7, 2018
Published online: October 27, 2018
Processing time: 158 Days and 5.5 Hours
Peer-review started: May 23, 2018
First decision: June 14, 2018
Revised: July 12, 2018
Accepted: August 6, 2018
Article in press: August 7, 2018
Published online: October 27, 2018
Processing time: 158 Days and 5.5 Hours
Core Tip
Core tip: This work describes the adaptation of a simplified bio-artificial liver (BAL) prototype to make it suitable to house rat liver microorgans (LMOs) as a biological component, and the evaluation of the performance in this new model. We demonstrate that the modification in the design of the artificial parts employed allows a good performance of LMOs, thus showing the importance of architecture and model configuration on the design of these devices. Besides its application as BAL, this mini bioreactor could serve as a suitable laboratory tool to evaluate the behavior and functionality of LMOs subjected to different incubation conditions due to its simple design and the utilization of standard materials.