Ohashi N, Hori T, Uemoto S, Jermanus S, Chen F, Nakao A, Nguyen JH. Hypothermia predicts hepatic failure after extensive hepatectomy in mice. World J Hepatol 2013; 5(4): 170-181 [PMID: 23671721 DOI: 10.4254/wjh.v5.i4.170]
Corresponding Author of This Article
Tomohide Hori, MD, PhD, Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, 54 Shogoinkawara-cho, Sakyo-ku, Kyoto 606-8507, Japan. horit@kuhp.kyoto-u.ac.jp
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World J Hepatol. Apr 27, 2013; 5(4): 170-181 Published online Apr 27, 2013. doi: 10.4254/wjh.v5.i4.170
Hypothermia predicts hepatic failure after extensive hepatectomy in mice
Norifumi Ohashi, Tomohide Hori, Shinji Uemoto, Sura Jermanus, Feng Chen, Akimasa Nakao, Justin H Nguyen
Norifumi Ohashi, Akimasa Nakao, Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Nagoya, Aichi 466-8550, Japan
Tomohide Hori, Shinji Uemoto, Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan
Sura Jermanus, Feng Chen, Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, United States
Justin H Nguyen, Division of Transplant Surgery, Department of Transplantation, Mayo Clinic, Jacksonville, FL 32224, United States
Author contributions: Nguyen JH designed this study; Ohashi N performed the surgery, the assays and statistical analysis, and wrote the initial draft; Hori T performed the additional surgery for the second assays to confirm the initial results; Jermanus S and Chen F assisted with the assays; Hori T and Nguyen JH contributed to further drafts; Uemto S and Nakao A provided important advice for this research; Nguyen JH supervised this research.
Supported by NIH DK064361, NIH R01NS051646 and the Deason Foundation, to Nguyen JH; the Uehara Memorial Foundation, No. 200940051, to Hori T
Correspondence to: Tomohide Hori, MD, PhD, Division of Hepato-Biliary-Pancreatic and Transplant Surgery, Department of Surgery, Kyoto University Graduate School of Medicine, 54 Shogoinkawara-cho, Sakyo-ku, Kyoto 606-8507, Japan. horit@kuhp.kyoto-u.ac.jp
Telephone: +81-75-7513111 Fax: +81-75-7513106
Received: February 20, 2012 Revised: November 24, 2012 Accepted: December 22, 2012 Published online: April 27, 2013
Abstract
AIM: To investigate the effect of hypothermia on the function of the liver remnant (LR) after extended hepatectomy.
METHODS: We performed a 75% partial hepatectomy (PH) in male C57BL/6J mice. Body temperature was measured with a rectal probe. The study mice were prospectively grouped as hypothermic (HT) or normothermic (NT) if their body temperature was < 34 °C vs≥ 34 °C, respectively. Blood and liver samples were obtained at 24 and 48 h after 75% PH. Various factors during and after 75% PH were compared at each time point and the most important factor for a good outcome after 75% PH was determined.
RESULTS: At 24 and 48 h after 75% PH, LR weight was decreased in HT mice compared with that in NT mice and the assay results in the HT mice were consistent with liver failure. NT mice had normal liver regeneration. Each intra- and post-operative factor which showed statistical significance in univariate analysis was evaluated by multivariate analysis. The most important factor for a good outcome after 75% PH was body temperature at both 24 and 48 h after surgery.
CONCLUSION: Hypothermia after an extensive hepatectomy predicts impending liver failure and may be a useful clinical marker for early detection of liver failure after extended hepatectomy.
