Ghanem LY, Dahmen U, Dirsch O, Nosseir MM, Mahmoud SS, Mansour WA. Does granulocyte-colony stimulating factor administration induce damage or repair response in schistosomiasis? World J Hepatol 2010; 2(12): 434-441 [PMID: 21191519 DOI: 10.4254/wjh.v2.i12.434]
Corresponding Author of This Article
Mona MF Nosseir, MD Pathology, Department of Pathology, Theodor Bilharz Research Institute, PO Box 30 Imbaba, Giza 12411, Egypt. drmonanosseir@yahoo.com
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World J Hepatol. Dec 27, 2010; 2(12): 434-441 Published online Dec 27, 2010. doi: 10.4254/wjh.v2.i12.434
Does granulocyte-colony stimulating factor administration induce damage or repair response in schistosomiasis?
Lobna Y Ghanem, Uta Dahmen, Olaf Dirsch, Mona MF Nosseir, Soheir S Mahmoud, Wafaa AF Mansour
Lobna Y Ghanem, Department of Electron Microscope, Theodor Bilharz Research Institute, PO Box 30 Imbaba, Giza 12411, Egypt
Uta Dahmen, Department of General, Visceral and Transplantation Surgery, University Hospital Essen, Hufelandstrasse 55, Essen D-45122, Germany
Olaf Dirsch, Division of Pathology, German Heart Institute, Berlin 13353, Germany
Mona MF Nosseir, Department of Pathology, Theodor Bilharz Research Institute, PO Box 30 Imbaba, Giza 12411, Egypt
Soheir S Mahmoud, Department of Parasitology, Theodor Bilharz Research Institute, PO Box 30 Imbaba, Giza 12411, Egypt
Wafaa AF Mansour, Department of Immunology, Theodor Bilharz Research Institute, PO Box 30 Imbaba, Giza 12411, Egypt
Author contributions: Ghanem LY, Dahmen U and Dirsch O were responsible for construction of the plan and design of work, discussion of results, writing the manuscript and financial support; Nosseir MMF for acquisition, interpretation, analysis and discussion of histopathological results, providing histopathological figures and revising the text critically; Mahmoud SS performed parasitological investigations and collected data; and Mansour WAF helped in discussion of the results and performance of laboratory investigations.
Correspondence to: Mona MF Nosseir, MD Pathology, Department of Pathology, Theodor Bilharz Research Institute, PO Box 30 Imbaba, Giza 12411, Egypt. drmonanosseir@yahoo.com
Telephone: +2-20-35401019 Fax: +2-20-35408125
Received: July 9, 2010 Revised: November 4, 2010 Accepted: November 11, 2010 Published online: December 27, 2010
Abstract
AIM: To introduce Granulocyte-colony stimulating factor (G-CSF) as a new therapeutic modality for schistosomiasis through stem cell mobilization, immunomodulation or fibrosis remodeling.
METHODS: In this study, a 5 d course of human recombinant G-CSF (100 μg/kg sc) was applied to Schistosoma mansoni-infected mice at different stages of disease (5 d before infection as well as 3, 5 and 7 wk post-infection). The animals were sacrificed at 10 d as well as 4, 6 and 8 wk post infection. Mice were examined for: (1) Total leukocyte count which is an accepted surrogate marker for the stem cell mobilization into the circulation; (2) Egg count in intestine and liver tissue to assess the parasitic load; and (3) Histopathological changes in Hx/E and Masson trichrome stained sections as well as collagen content in Sirius red-stained liver sections to determine the severity of liver fibrosis.
RESULTS: Mice developed leukocytosis. The egg load and the number of granulomas were not affected by the G-CSF treatment but there was an obvious change in the composition of granulomas towards an increased cellularity. Moreover, fibrosis was significantly decreased in treated groups compared to untreated animals (collagen content either preinfection or at 3 and 5 wk post infection: 5.8 ± 0.5, 4.7 ± 0.5, 4.0 ± 0.7 vs 8.2 ± 0.9; P≤ 0.01).
CONCLUSION: Although G-CSF did not cause direct elimination of the parasite, it enhanced granulomatous reaction and reduced the fibrosis. Further investigation of the underlying mechanisms of these two actions is warranted.
