Basic Study
Copyright ©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Hepatol. Aug 27, 2024; 16(8): 1167-1176
Published online Aug 27, 2024. doi: 10.4254/wjh.v16.i8.1167
Gadoxetic acid-enhanced magnetic resonance imaging in the assessment of hepatic sinusoidal obstruction syndrome in a mouse model
Yuan-Yuan Chen, Li Yang, Jun Li, Sheng-Xiang Rao, Ying Ding, Meng-Su Zeng
Yuan-Yuan Chen, Li Yang, Jun Li, Sheng-Xiang Rao, Ying Ding, Meng-Su Zeng, Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Author contributions: Chen YY contributed to the conception, design, acquisition, analysis, interpretation of data, wrote the manuscript; Yang L and Li J collected and preprocessed the data; Rao SX provided supervision and methodology; Ding Y revised the manuscript and gave the final approval of the version to be published; Zeng MS was responsible for conceptualization and supervision.
Supported by the National Science Foundation for Young Scientists of China, No. 81701682.
Institutional animal care and use committee statement: Animal experimental procedures were reviewed and approved by Zhongshan Hospital Institutional Ethics and Animal Care on February 4, 2017.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The data that support the findings of this study are available from the corresponding author upon reasonable request at ding.ying@zs-hospital.sh.cn.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Ying Ding, MD, Associate Chief Physician, Department of Radiology, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai 200032, China. ding.ying@zs-hospital.sh.cn
Received: May 15, 2024
Revised: July 13, 2024
Accepted: August 2, 2024
Published online: August 27, 2024
Processing time: 97 Days and 23.7 Hours
Abstract
BACKGROUND

Neoadjuvant chemotherapy can cause hepatic sinusoidal obstruction syndrome (SOS) in patients with colorectal cancer liver metastases and increases postoperative morbidity and mortality.

AIM

To evaluate T1 mapping based on gadoxetic acid-enhanced magnetic resonance imaging (MRI) for diagnosis of hepatic SOS induced by monocrotaline.

METHODS

Twenty-four mice were divided into control (n = 10) and experimental (n = 14) groups. The experimental groups were injected with monocrotaline 2 or 6 days before MRI. MRI parameters were: T1 relaxation time before enhancement; T1 relaxation time 20 minutes after enhancement (T1post); a reduction in T1 relaxation time (△T1%); and first enhancement slope percentage of the liver parenchyma (ESP). Albumin and bilirubin score was determined. Histological results served as a reference. Liver parenchyma samples from the control and experimental groups were analyzed by western blotting, and organic anion transporter polypeptide 1 (OATP1) was measured.

RESULTS

T1post, △T1%, and ESP of the liver parenchyma were significantly different between two groups (all P < 0.001) and significantly correlated with the total histological score of hepatic SOS (r = -0.70, 0.68 and 0.79; P < 0.001). △T1% and ESP were positively correlated with OATP1 levels (r = 0.82, 0.85; P < 0.001), whereas T1post had a negative correlation with OATP1 levels (r = -0.83; P < 0.001).

CONCLUSION

T1 mapping based on gadoxetic acid-enhanced MRI may be useful for diagnosis of hepatic SOS, and MRI parameters were associated with OATP1 levels.

Keywords: T1 mapping; Gadoxetic acid; Sinusoidal obstruction syndrome; Organic anion transporter polypeptides; Magnetic resonance imaging

Core Tip: Neoadjuvant chemotherapy could cause hepatic sinusoidal obstruction syndrome (SOS) in colorectal liver metastases patients and increases postoperative morbidity and mortality. We used a mouse model of monocrotaline-induced hepatic SOS. We confirmed the relationship between magnetic resonance imaging (MRI) parameters T1 relaxation time 20 minutes after enhancement, a reduction in T1 relaxation time and first enhancement slope percentage of the liver parenchyma and hepatic SOS, suggesting a potential method for the assessment of hepatic SOS. Our results also demonstrated that MRI parameters were correlated with organic anion transporter polypeptide 1 levels.