Copyright ©The Author(s) 2015.
World J Stem Cells. May 26, 2015; 7(4): 681-690
Published online May 26, 2015. doi: 10.4252/wjsc.v7.i4.681
Table 1 Studies describing in vitro modification of standard culture condition to induce osteogenic differentiation of AF-derived cells
Cell sourceMethods to induce osteogenic differentiationRef.
hAF-MSCsCulture in standard osteogenic medium on sandblasted and acid etching titanium (SLA titanium)[60]
hAF-MSCsTransfection with a defective adenoviral vector expressing human LMP3[63]
hAFSCsStandard osteogenic medium plus curculigoside[65]
hAFSCsStandard osteogenic medium plus naringin[66]
hAFSCs100 µmol/L 2P-ascorbic acid, 100 nmol/L dexamethasone, supplemented with different concentrations of Ferutinin[67]
hAF-MSCsInduction of miR-21[71]
hAFSCsMedium containing 50 nmol/L rhBMP-7, 50 mg/mL ascorbic acid, and 10 mmol/L b-glycerophosphate on nanofibrous or solid walled scaffolds[86]
hAFSCs10 nmol/L dexamethasone, 6 mmol/L β-glycerol phosphate, 50 mg/mL ascorbic acid 2-phosphate, 50 ng/mL L-thyroxine on electrospun nanofiber meshes[88]
hAFSCsStandard osteogenic medium on: (1) Fibroin scaffold; (2) Collagen scaffold; and (3) Poly-D,L-lactic acid scaffold;[89]
hAFSCsStandard osteogenic medium on microfibrous starch and poly(ε-caprolactone) scaffold[90]
hAFSCsMedical-grade poly-ε-caprolactone scaffold[91]
hAFSCsStandard osteogenic medium on collagen matrix derived from porcine bladder submucosa matrix and poly (lactide-co-glycolide)[92]
hAFSCsStandard osteogenic medium on construct composed of collagen type I[93]
hAFSCsα-MEM plus 17% FBS, 1 μmol/L dexamethasone, 6 mmol/L of β-glycerol phosphate, 50 μg/mL ascorbic acid 2-phosphate, and 50 ng/mL thyroxine on biomaterial named Skelite[38]
Table 2 Studies describing in vivo osteogenic differentiation of AF-derived cells
Cell sourceScaffoldAnimal modelRef.
oAF-MSCsMagnesium-enriched hydroxyapatite/collagen-based scaffoldSheep model of sinus augumentation[85]
hAFSCsNanofibrous or solid walled scaffoldsMidsagittal incision made on the dorsa and two subcutaneous pockets created using blunt dissection on male outbred thymic nude mouse model (nu/nu)[86]
hAFSCsFibroin scaffolds compared to collagen and poly-D,L-lactic acid scaffoldsDorsal mid-sagittal incision made on the dorsa and two subcutaneous pockets were created using blunt dissection male outbred rats[89]
hAFSCsMicrofibrous starch and poly(ε-caprolactone) scaffoldSubcutaneous implantation into the dorsal side of athymic female nude rats[91]
hAFSCsConstruct composed of collagen type ICritical-sized rat calvarial defect after oral administration of phytoestrogen ferutinin[93]
hAFSCsBiomaterial SkeliteSubcutaneous implantation in the back of CD-1 nu/nu mice[38]