Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis: Implications for bone health

Table 1 Genotyping primers

Primers	Sequence	Amplicon
HIF2α	F: 5’-GAGAGCAGCTTCTCCTGGAA-3’	WT182 bp
	R: 5’-TGTAGGCAAGGAAACCAAGG-3’	MT220 bp
Prx-1	F: 5’-GCGGTCTGGCAGTAAAAACTATC-3’	100 bp
	R: 5’-GTGAAACAGCATTGCTGTCACTT-3’

Table 2 Primers used for quantitative real-time polymerase chain reaction of total RNA

Genes	Primers types	Sequence (5’-3’)
HIF-2α	Forward	CTCCCACCTGGACAAAGC
	Reverse	GTCGCAAGGATGAGTGAAGT
HIF-2α exon2	Forward	ACAAAGCCTCCATCATGCG
	Reverse	CGTCTTGGGTCACCACAG
Runx2	Forward	TCCACAAGGACAGAGTCAGATTACAG
	Reverse	CAGAAGTCAGAGGTGGCAGTGTCATC
Col1α1	Forward	TGTGTGCGATGACGTGCAAT
	Reverse	GGGTCCCTCGACTCCTACA
OCN	Forward	AGACTCCGGCGCTACCTT
	Reverse	AAGCAGGGTCAAGCTCACAT
CEBPa	Forward	CAAGAACAGCAACGAGTACCG
	Reverse	GTCACTGGTCAACTCCAGCAC
Lpl	Forward	GCGTAGCAGGAAGTCTGACCAA
	Reverse	AGCGTCATCAGGAGAAAGGCGA
Adipo-nectin	Forward	TGTTCCTCTTAATCCTGCCCA
	Reverse	CCAACCTGCACAAGTTCCCTT
PI3K	Forward	ACACCACGGTTTGGACTATGG
	Reverse	GGCTACAGTAGTGGGCTTGG
AKT	Forward	TGGGTCAAGGAACAGAAGCA
	Reverse	TCACACTGACCACTGACACA
mTOR	Forward	CGGGACTCTTTACACTGCG
	Reverse	CCTTCAGGCTCAACCAACA
Actin	Forward	TTGCTGACAGGATGCAGAAGGAGA
	Reverse	ACTCCTGCTTGCTGATCCACATCT

HIF-2α: Hypoxia-inducible factor 2alpha; mTOR: Mechanistic target of rapamycin.