Evaluation of genetic response of mesenchymal stem cells to nanosecond pulsed electric fields by whole transcriptome sequencing

Table 1 Primer sequences used in the study

Primer	Primer sequence (5’ to 3’)
Actg2-F	CTCTCTCCACCTTCCAGCAAA
Actg2-R	AGGGCCCCGCTTCATC
Nek2-F	TGGAGGGCCTGACAATCTG
Nek2-R	CCACCACACTGAGTTTCTGGTTT
Cenpf-F	GGAGAGCCTGTGTGCATGTG
Cenpf-R	ACGTGAGCAGGAGGTTATGAAAC
Scin-F	TGGCCGAAGATGATGTCATG
Scin-R	CTTTGCCAATCCAAATGAAGATC
Kif20b-F	TGTGCCACACCAGTCACAATT
Kif20b-R	ACCTCGCCACTCTTCCTCTTC
Nog-F	CGAGGGTTTTCAATGAACTTTTTT
Nog-R	AGTGCATTACATGAACCAGAAAGC
Ereg-F	GGGTTGCCACAAGTCTGAACA
Ereg-R	GCATGCTGCACATCCTTGTC
Asic3-F	GCCTGCTTACCATCCTTGAGA
Asic3-R	CCCCAGGACTCTGTCTTGGA
Aldh3a1-F	TCCCACCGCCGCTCTT
Aldh3a1-R	GCCTTGTGAGCTTCTTCATTCA
Tubb2b-F	GGCGAGGATGAGGCTTGA
Tubb2b-R	TTCACCTCAGCTTTCCCTAACC
Cryba4-F	CGTGCTGGAGAGCGATCA
Cryba4-R	AGCCCCACTCCCTGAAGTG
Nr3c2-F	CGCACAGCAATATGAAAACCA
Nr3c2-R	GCCCCTTTCCCCCAGAA
Stxbp5l-F	AAGCCTCAGCAGGAAAAGCA
Stxbp5l-R	TGCCCGGTCCAGGAATG
Actin-F	TCTGTGTGGATTGGTGGCTCTA
Actin-R	CTGCTTGCTGATCCACATCTG

Table 2 Top 20 differentially expressed long noncoding RNAs

LncRNA_ID	Locus	Log2FC	Q value
NONRATT027355.2	7:143840739-143841394	6.223946	0.038374
NONRATT014734.2	19:44137444-44139463	6.103516	0.001285
NONRATT000391.2	1:78818388-78818948	6.035452	0.008784
NONRATT030693.2	X:115351001-115352258	5.611966	0.011922
MSTRG.1686.44	1:180852586-181353774	5.574714	0.004533
NONRATT029722.2	9:113936538-113936864	5.563687	0.013263
MSTRG.1686.24	1:180804226-181295177	5.546189	0.000326
NONRATT024954.2	6:50889024-50923726	5.468331	7.04E-05
NONRATT010080.2	14:83648401-83660792	5.356187	0.021489
NONRATT021215.2	4:29083076-29092661	-5.06373	0.022244
NONRATT015697.2	2:211078318-211078606	-5.08835	0.01522
NONRATT002358.2	1:80335470-80336633	-5.27782	0.011922
NONRATT015446.2	2:188562082-188565332	-5.7896	7.11E-08
NONRATT026659.2	7:30291089-30293287	-5.84278	0.001419
NONRATT017622.2	20:7219444-7220293	-5.89986	4.44E-16
NONRATT031234.2	X:116752819-116754309	-5.9906	0.021489
NONRATT024688.2	6:136358084-136363672	-6.06997	0.018677
NONRATT024848.2	6:25912732-26051235	-7.01177	0.000482
NONRATT005942.2	10:84682448-84688899	-7.27093	0.016558
NONRATT002900.2	X:157319040-157323878	-11.8256	3.16E-09

LncRNA: Long noncoding RNA; FC: Fold change.

Table 3 Top 20 differentially expressed messenger RNAs

Gene ID	Gene name	Log2FC	Q value
ENSRNOG00000017609	Cnga4	4.049502	0.041927
ENSRNOG00000005883	Nek10	3.164517	0.001515
ENSRNOG00000051612	AABR07044570.1	3.006014	0.020392
ENSRNOG00000003891	Porf1	2.826433	0.000369
ENSRNOG00000042070	Ticam2	2.821013	0.00501
ENSRNOG00000046566	Tub	2.803217	0.006364
ENSRNOG00000032973	Il13ra2	2.661856	1.68E-08
ENSRNOG00000025261	AABR07050407.1	2.232324	0.00943
ENSRNOG00000001959	Mx1	2.073926	0.049836
ENSRNOG00000031598	Atp8b4	2.055746	0.024695
ENSRNOG00000003283	Rcsd1	-2.09387	0.018919
ENSRNOG00000010454	Ccno	-2.1785	0.011229
ENSRNOG00000002456	Hlf	-2.44194	0.021066
ENSRNOG00000052129	Nwd1	-2.45693	0.032717
ENSRNOG00000014424	RGD1563354	-2.46926	0.016841
ENSRNOG00000049115	Ccr5	-2.73203	0.01083
ENSRNOG00000055401	Kcnc1	-3.13004	0.021817
ENSRNOG00000055318	AABR07068030.1	-3.34625	0.000321
ENSRNOG00000014556	Cdh20	-3.47543	0.025307
ENSRNOG00000054723	AABR07058174.1	-3.5912	0.047547
ENSRNOG00000011946	Ptn	-3.6805	0.000634

FC: Fold change.