Silencing of Jumonji domain-containing 1C inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells via nuclear factor-κB signaling

Table 1 The primer sequences used in reverse transcription coupled to the quantitative polymerase chain reaction

Gene	Species	Direction	Sequence	Tm	Product lengths	Accession
RunX2	Mouse	Forward primer	AGAGTCAGATTACAGATCCCAGG	60.12	109 bp	90-112
		Reverse primer	TGGCTCTTCTTACTGAGAGAGG	59.66		198-178
Osteocalin	Mouse	Forward primer	CTGACCTCACAGATCCCAAGC	61.54	114 bp	66-85
		Reverse primer	TGGTCTGATAGCTCGTCACAAG	59		179-160
ALP	Mouse	Forward primer	CCAACTCTTTTGTGCCAGAGA	60.2	110 bp	44-64
		Reverse primer	GGCTACATTGGTGTTGAGCTTTT	61.5		153-131
PPAR-γ	Mouse	Forward primer	GGAAGACCACTCGCATTCCTT	62.1	121 bp	69-89
		Reverse primer	GTAATCAGCAACCATTGGGTCA	60.6		189-168
CEBP-α	Mouse	Forward primer	GCGGGAACGCAACAACATC	61.2	124 bp	840-860
		Reverse primer	GTCACTGGTCAACTCCAGCAC	62.9		963-943
JMJD1C	Mouse	Forward primer	ACATCACGGCGAAGGTCTC	60.4	87 bp	872-893
		Reverse primer	TGGGACTATTTGCTTGAGCAC	61.5		958-938
IL-1β	Mouse	Forward primer	GGTGCTGATGTACCAGTT	60.5	95 bp	66-86
		Reverse primer	TCTATACCACTTCACAAGTCGGA	61.0		186-165
IL-6	Mouse	Forward primer	GAATTGCCATTGCACAACTCTTT	60.9	105 bp	642-662
		Reverse primer	GTCACTGGTCAACTCCAGCAC	61.3		765-746
TNF-α	Mouse	Forward primer	GAACCTTTCTGGCCCGTGT	60.8	93 bp	770-791
		Reverse prime	AGAAATCGCAATTCATGTCGCA	61.2		856-336
GAPDH	Mouse	Forward primer	GACGTGCCGCCTGGAGA	62.5	95 bp	8-28
		Reverse prime	GAAGAGTGGGAGTTGCTGTTGAA	62.5		102-84

RunX2: Runt-related transcription factor 2; ALP: Alkaline phosphatase; PPARγ: Peroxisome proliferator-activated receptor gamma; CEBPα: CCAAT enhancer-binding protein alpha; JMJD1C: Jumonji domain-containing 1C; TNF-α: Tumor necrosis factor alpha; IL: Interleukin; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.