Basic Study
Copyright ©The Author(s) 2022.
World J Stem Cells. Aug 26, 2022; 14(8): 599-615
Published online Aug 26, 2022. doi: 10.4252/wjsc.v14.i8.599
Table 1 Primer sequences of polymerase chain reaction
Gene
Primer sequences
Dppa3F: 5′-TGTGGAGAACAAGAGTGA-3′
R: 5′-CTCAATCCGAACAAGTCTT-3′
NanogF: 5′-CTCAAGTCCTGAGGCTGACA-3′
R: 5′-TGAAACCTGTCCTTGAGTGC-3′
Rex1F: 5′-CAGCCAGACCACCATCTGTC-3′
R: 5′-GTCTCCGATTTGCATATCTCCTG-3′
endo-Sox2 F: 5′-AGGGCTGGGAGAAAGAAGAG-3′
R: 5′-CCGCGATTGTTGTGATTAGT-3′
endo-Klf4 F: 5′-AACATGCCCGGACTTACAAA-3′
R: 5′-TTCAAGGGAATCCTGGTCTTC-3′
endo-Oct4F: 5′-TAGGTGAGCCGTCTTTCCAC-3′
R: 5′-GCTTAGCCAGGTTCGAGGAT-3′
endo-c-MycF: 5′-TAACTCGAGGAGGAGCTGGA-3′
R: 5′-GCCAAGGTTGTGAGGTTAGG-3′
exo-Sox2F: 5′-GGGTGGACCATCCTCTAGAC-3′
R: 5′-GGGCTGTTCTTCTGGTTG-3′
exo-Klf4F: 5′-GGGTGGACCATCCTCTAGAC-3′
R: 5′-GCTGGACGCAGTGTCTTCTC-3′
exo-Oct4F: 5′-GGGTGGACCATCCTCTAGAC-3′
R: 5′-CCAGGTTCGAGAATCCAC-3′
exo-c-MycF: 5′-GGGTGGACCATCCTCTAGAC-3′
R: 5′-CCTCGTCGCAGATGAAATAG-3′
β-actinF: 5′GTGGGCCGCTCTAGGCACCAA-3′
R: 5′CTCTTTGATGTCACGCACGATTTC-3′