Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway

doi:10.4252/wjsc.v13.i12.1928

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Dec 26, 2021 (publication date) through Dec 17, 2024

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World Journal of Stem Cells

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1948-0210

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World J Stem Cells. Dec 26, 2021; 13(12): 1928-1946
Published online Dec 26, 2021. doi: 10.4252/wjsc.v13.i12.1928

Table 1 Sequences of primers used for real-time PCR

Gene	Primer sequence
GAPDH	Forward 5'-CTGGAGAAACCTGCCAAGTATG-3'Reverse 5'-GGTGGAAGAATGGGAGTTGCT-3'
P16	Forward 5'-CCGATACAGGTGATGATGATGG-3'Reverse 5'-CGGAGGAGAGTAGATACCGCAAA-3'
P21	Forward 5'-AGTTGGAGCTGGTGGCGTAG-3'Reverse 5'-AATACACAAAGAAAGCCCTCCC-3'
SIRT1	Forward 5'-AGATTTCAAGGCTGTTGGTTCC-3'Reverse 5'-CAGCATCATCTTCCAAGCCATT-3'
PGC-1α	Forward 5'-GAGAAGCGGGAGTCTGAAAGG-3'Reverse 5'-GTCACAGGTGTAACGGTAGGTAATG-3'

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; SIRT1: Silent information regulator of transcription 1; PGC-1α: PPAR gamma coactivator-1α.

Citation: Shi PZ, Wang JW, Wang PC, Han B, Lu XH, Ren YX, Feng XM, Cheng XF, Zhang L. Urolithin a alleviates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells through SIRT1/PGC-1α pathway. World J Stem Cells 2021; 13(12): 1928-1946
URL: https://www.wjgnet.com/1948-0210/full/v13/i12/1928.htm
DOI: https://dx.doi.org/10.4252/wjsc.v13.i12.1928