ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells

Table 1 Percentage of immunostained cells of each sample for neural markers at day 1 and day 8 of culture

Marker	Day 1				Day 8
	ASCs	sfASCs	F12/ASCs	CM/ASCs	ASCs	sfASCs	F12/ASCs	CM/ASCs
Nestin	62 ± 5	53 ± 4	51 ± 3	78 ± 4	74 ± 6	39 ± 2	31 ± 3	88 ± 4
NSE	63 ± 3	52 ± 6	54 ± 7	82 ± 3	61 ± 4	49 ± 3	50 ± 4	93 ± 5
PGP9.5	68 ± 4	65 ± 3	66 ± 5	75 ± 5	78 ± 5	62 ± 3	67 ± 6	89 ± 3
GFAP	55 ± 7	56 ± 5	53 ± 4	77 ± 3	53 ± 6	41 ± 5	45 ± 7	87 ± 5

ASCs: Control ASCs cultured in basal DMEM; CM/ASCs: ASCs cultured in serum-free DMEM/F12 conditioned from ARPE-19; F12/ASCs: ASCs cultured in serum-free DMEM/F12; sfASCs: ASCs cultured in serum-free DMEM. GFAP: Glial fibrillary acidic protein; NSE: Neuron specific enolase; PGP9.5: Protein gene product 9.5; ASCs: Adipose-derived stem cells; sfASCs: Serum-free adipose-derived stem cells; CM: Conditioned medium.