Effect of glycyrrhizic acid and 18β-glycyrrhetinic acid on the differentiation of human umbilical cord-mesenchymal stem cells into hepatocytes

Table 1 Primer sequences of the targeted genes

Genes	Primer sequence (5’-3’)	Annealing temperature (°C)
Beta- Actin	Forward: 5’-CACTGGCATCGTGATGGACT-3’	60
	Reverse: 5’-TGGCCATCTCTTGCTCGAAG-3’
Alpha-Fetoprotein	Forward: 5’-CAGCATCGATCCCACTTTTCC-3’	62
	Reverse: 5’-ATTTTGTCATAGCGAGCAGCC-3’
Albumin	Forward: 5’-CAAAGCATGGGCAGTGCTC-3’	60
	Reverse: 5’-GCCCTGTCATCAGCACATTC-3’
Cytokeratin-18	Forward: 5’-CCAGCTTGGAGAACAGCCT-3’	60
	Reverse: 5’-AGCCTCCAGCTTGACCTTG-3’
Tyrosine-aminotransferase	Forward: 5’-TCTGAGCTTCCTCAAGTCCAA-3’	63
	Reverse: 5’-CATGAGGTCATAGCCCCAGA-3’
Cytochrome P450 2B6	Forward: 5’-ATTGTCACCCAACACACCAG -3’	60
	Reverse: 5’-CAGTCTTTTTCAGTGCCCCA -3’
Hepatocyte nuclear; factor 4-α	Forward: 5’-GACTACATTGTCCCTCGGCA -3’	62
	Reverse: 5’-ATACTGGCGGTCGTTGATGT -3’

Table 2 qRT-PCR program layout

Process	Temperature (°C)	Time duration	Cycles
Initial denaturation	95	10 min	1
Cyclic denaturation	95	15 s	40
Annealing and extension	60	1 min
Melting curve	95	15 s	-
	60	15 s
	95	15 s