Copyright
©The Author(s) 2020.
World J Stem Cells. May 26, 2020; 12(5): 359-367
Published online May 26, 2020. doi: 10.4252/wjsc.v12.i5.359
Published online May 26, 2020. doi: 10.4252/wjsc.v12.i5.359
PBSC unit | Unit characteristics | Pre-freeze characteristics | Post-thaw characteristics | Colony forming ability | ||||||
Donor age (yr) | Disease | Cryopreser-vation (yr) | TNC × 103/µL | %CD34 | TNC × 103/µL | %TNC Recovery | %Viability | BFU-E/105 | CFU-GM/106 | |
A | 25 | GLIO | 18.1 | 110 | 0.2 | 121 | 110 | 84 | 2 | 2 |
B | 58 | CML | 17.7 | 120 | 0.5 | 111 | 103 | 86 | 6 | 10 |
C | 46 | MM | 13.6 | 120 | 3.9 | 116 | 81 | 68 | ++ | ++ |
D | 24 | NHL | 14.6 | 116 | 3.8 | 110 | 68 | 67 | ++ | ++ |
E | 52 | MM | 17.9 | 127 | 0.2 | 164 | 90 | 86 | 0.3 | 0 |
F | 66 | MM | 17 | 102 | 0.5 | 145 | 95 | 34 | 0 | 0 |
G | 54 | MM | 18.3 | 134 | 0.5 | 114 | 79 | 81 | 4 | 6 |
H | 52 | MM | 17.9 | 120 | 0.2 | 150 | 90 | 70 | 9 | 6 |
I | 47 | MM | 17.2 | 114 | 1.5 | 120 | 80 | 62 | 0.3 | 0 |
J | 47 | MM | 17.1 | 114 | 1.5 | 124 | 83 | 49 | ++ | ++ |
PBSC unit | Cryopreser-vation (yr) | Post-CD34 selection characteristics | Mouse bone marrow engraftment | |||||||
TNC × 103/µL | %Viability | %Purity | BFU-E/105 | CFU-GM/106 | Mean %CD45 | Mean %CD19 | Mean %CD3 | Mean %CD33 | ||
A | 18.1 | 0.3 | 96 | 95 | 19 | 56 | 43.4 | 14.8 | 1.1 | 12.6 |
B | 17.7 | 0.3 | 94 | 93 | 54 | 85 | 63.6 | 20.8 | 0.4 | 13.1 |
C | 13.6 | 2.5 | 96 | 98 | ++ | ++ | 10.3 | 2.8 | 1 | 5.6 |
D | 14.6 | 2.4 | 95 | 98 | ++ | ++ | 20.3 | 6.8 | 0.5 | 7.8 |
- Citation: Underwood J, Rahim M, West C, Britton R, Skipworth E, Graves V, Sexton S, Harris H, Schwering D, Sinn A, Pollok KE, Robertson KA, Goebel WS, Hege KM. How old is too old? In vivo engraftment of human peripheral blood stem cells cryopreserved for up to 18 years - implications for clinical transplantation and stability programs. World J Stem Cells 2020; 12(5): 359-367
- URL: https://www.wjgnet.com/1948-0210/full/v12/i5/359.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v12.i5.359