Basic Study
Copyright ©The Author(s) 2020.
World J Stem Cells. May 26, 2020; 12(5): 359-367
Published online May 26, 2020. doi: 10.4252/wjsc.v12.i5.359
Table 1 Cryopreserved peripheral blood stem cells unit characteristics
PBSC unitUnit characteristics
Pre-freeze characteristics
Post-thaw characteristics
Colony forming ability
Donor age (yr)DiseaseCryopreser-vation (yr)TNC × 103/µL%CD34TNC × 103/µL%TNC Recovery%ViabilityBFU-E/105CFU-GM/106
A25GLIO18.11100.21211108422
B58CML17.71200.511110386610
C46MM13.61203.91168168++++
D24NHL14.61163.81106867++++
E52MM17.91270.216490860.30
F66MM171020.5145953400
G54MM18.31340.5114798146
H52MM17.91200.2150907096
I47MM17.21141.512080620.30
J47MM17.11141.51248349++++
Table 2 Post-CD34 selection characteristics and engraftment data
PBSC unitCryopreser-vation (yr)Post-CD34 selection characteristics
Mouse bone marrow engraftment
TNC × 103/µL%Viability%PurityBFU-E/105CFU-GM/106Mean %CD45Mean %CD19Mean %CD3Mean %CD33
A18.10.39695195643.414.81.112.6
B17.70.39493548563.620.80.413.1
C13.62.59698++++10.32.815.6
D14.62.49598++++20.36.80.57.8

  • Citation: Underwood J, Rahim M, West C, Britton R, Skipworth E, Graves V, Sexton S, Harris H, Schwering D, Sinn A, Pollok KE, Robertson KA, Goebel WS, Hege KM. How old is too old? In vivo engraftment of human peripheral blood stem cells cryopreserved for up to 18 years - implications for clinical transplantation and stability programs. World J Stem Cells 2020; 12(5): 359-367
  • URL: https://www.wjgnet.com/1948-0210/full/v12/i5/359.htm
  • DOI: https://dx.doi.org/10.4252/wjsc.v12.i5.359