Prospects for the therapeutic development of umbilical cord blood-derived mesenchymal stem cells

Table 1 New culture condition of umbilical cord blood-derived mesenchymal stem cells

Subject	Conditions	Title	Year
Hypoxia	5 %	Effects of hypoxia on proliferation of human cord blood-derived mesenchymal stem cells.	2016
	1 %	Protein profiling and angiogenic effect of hypoxia-cultured man umbilical cord blood-derived mesenchymal stem cells in hindlimb ischemia.	2017
	2.5 %	The effect of hypoxia preconditioning on the neural and stemness genes expression profiling in human umbilical cord blood mesenchymal stem cells.	2017
	1 %	Hypoxia preconditioning increases survival and pro-angiogenic capacity of human cord blood mesenchymal stromal cells in vitro.	2015
3D	Spheroid	Up-regulation of superoxide dismutase 2 in 3D spheroid formation promotes therapeutic potency of human umbilical cord blood-derived mesenchymal stem cells.	2020
	Spheroid	effect on multipotency and phenotypic transition of unrestricted somatic stem cells from human umbilical cord blood after treatment with epigenetic agents	2016
	Collagen sponge	Chondrogenic commitment of human umbilical cord blood-derived mesenchymal stem cells in collagen matrices for cartilage engineering.	2016
	Collagen constructs	Enhanced survival and neurite network formation of human umbilical cord blood neuronal progenitors in three-dimensional collagen constructs.	2013
	Spheroid	Spherical bullet formation via E-cadherin promotes therapeutic potency of MSCs derived from human umbilical cord blood for myocardial infarction.	2012
Small size	8 µm size ≤	A small-sized population of human umbilical cord blood-derived mesenchymal stem cells shows high stemness properties and therapeutic benefit.	2020
CoCl2	100 µmol/L	Cobalt Chloride Enhances the Anti-Inflammatory Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells through the ERK-HIF-1α-MicroRNA-146a-Mediated Signaling Pathway	2018
Combine condition	Ca2+ (1.8 mmol/L) and hypoxia (3%)	Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells.	2017
	Size (10 µm ≤) and Ca2+ (1.8 mmol/L) and hypoxia (3%)	Small hypoxia-primed mesenchymal stem cells attenuate graft-versus-host disease.	2018
	Hypoxia (5%) and 3D (collagen sponge)	Hypoxia is a critical parameter for chondrogenic differentiation of human umbilical cord blood mesenchymal stem cells in Type I/III collagen sponges	2017
	IL-1β (5 ng/mL) andIFNλ (20 ng/mL)	Preconditioning with interleukin-1 beta and interferon-gamma enhances the efficacy of human umbilical cord blood-derived mesenchymal stem cells-based therapy via enhancing prostaglandin E2 secretion and indoleamine 2,3-dioxygenase activity in dextran sulfate sodium-induced colitis.	2019
Gene overexpression	HGF and VEGF (TALEN)	Coronary stents with inducible VEGF/HGF secreting UCB-MSCs reduced restenosis and increased reendothelialization in a swine model.	2018
	LEF-1 (adenoviral)	Transplantation of hMSCs genome edited with lef1 improves cardio-protective effects in myocardial infarction.	2020
	LIGHT (lentiviral)	Gene therapy of gastric cancer using LIGHT-secreting human umbilical cord blood-derived mesenchymal stem cells.	2013
	BMP-2 (lentiviral)	Lentiviral gene therapy for bone repair using human umbilical cord blood–derived mesenchymal stem cells.	2019
	BMP-2 (non-viral)	Transfection of hBMP-2 into mesenchymal stem cells derived from human umbilical cord blood and bone marrow induces cell differentiation into chondrocytes.	2014
	FGF-20 (adenoviral)	The effect of MSCs derived from the human umbilical cord transduced by fibroblast growth factor-20 on Parkinson’s disease	2016

ERK: Extracellular signal-regulated kinase; HIF: Hypoxia-inducible factor; VEGF: Vascular endothelial growth factor; HGF: Hepatocyte growth factor; TALEN: Transcription activator-like effector nuclease; UCB: Umbilical cord blood; MSC: Mesenchymal stem cell; LEF: Lymphoid enhancer-binding factor; LIGHT: TNFSF14 (tumor necrosis factor superfamily member 14); BMP: Bone morphogenetic protein; FGF: Fibroblast growth factor.