Case Report
Copyright ©The Author(s) 2019.
World J Stem Cells. Feb 26, 2019; 11(2): 124-146
Published online Feb 26, 2019. doi: 10.4252/wjsc.v11.i2.124
Table 1 Overview on the treatments performed in the present study.
TreatmentsTime pointProcedures
Pre-phaseT0Evaluation of the patient
Preparatory stepsT1 (4 mo after T0)Preparation of plasma rich in growth factors (PRGF-2); isolation of unmodified, autologous adipose derived regenerative cells (UA-ADRCs)
1T1 (GBR-MSA)Extraction of tooth # 14; ridge preservation and external sinus lift procedures
2T2 (6 wk after T1)Extraction of teeth # 11, #12 and #22; collection of the first biopsies
3T3 (34 wk after T1)Placement of implants; collection of the second biopsies
4T4 (1 yr after T3)Extraction of tooth #18; placement of healing abutments
5T5 (6 wk after T4)Placement of the definitive prosthetic telescopic bridge
T6 (32 mo after T1)Last radiologic follow-up
Table 2 Details of antibodies and incubation protocols used in the present study
runX2Goat polyclonalSanta Cruz Biotechnology (Dallas, TX, United States)1:30, 4 °C, ON
Collagen type IRabbit monoclonalAbcam (Cambridge, United Kingdom)1:400, RT, 1 h
Alkaline phosphataseRabbit polyclonalQuartett (Berlin, Germany)Ready to use, 4 °C, ON
Von Willebrand factorRabbit polyclonalLinaris (Dossenheim, Germany)1:200, RT, 1h
CD146Rabbit monoclonalAbcam1:50, RT, 1h
CD73Mouse monoclonalAntibodies-online (Atlanta, GA, United States)1:100, 4 °C, ON
PPARγMouse monoclonalSanta Cruz Biotechnology1:25, 4 °C, ON