Brief Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Stem Cells. Jan 26, 2013; 5(1): 34-42
Published online Jan 26, 2013. doi: 10.4252/wjsc.v5.i1.34
Figure 1
Figure 1 Morphology of mouse bone marrow cultures. Cells shown here represent passages 0-19 of bone marrow cells from CBA/Ca, ICR and Balb/c mice. Original magnification: × 200.
Figure 2
Figure 2 Bone marrow cultures acquire CD106+CD45- immunophenotype at later passages. Bottom right quadrants show percentage of CD106+CD45- cells for mesenchymal stem cells of CBA/Ca, ICR and Balb/c mice. Numbers in the upper right region indicate passage number.
Figure 3
Figure 3 Bone marrow cultures show decreased CD11b and increased Sca-1 expression at later passages. Histograms show the shift of CD11b+ at earlier passages to CD11b- at later passages for each strain of mice. Expression of Sca-1 is increased with mesenchymal stem cells passages.
Figure 4
Figure 4 Mesenchymal stem cells cultures from all 3 strains of mice show positivity to CD44 and MHC I but negativity to MHC II. Numbers within plots indicate the percentage of positivity to each marker.
Figure 5
Figure 5 Mesenchymal stem cells from all 3 strains of mice differentiate into adipocytes and osteocytes. First and second row: images of non-induced mesenchymal stem cells (MSC) control and MSC differentiated into adipocytes, stained with Oil Red O. Third and fourth row: images of non-induced MSC control and MSC differentiated into osteocytes, stained with Alizarin Red S. Differences in quality of images are because they were taken using different camera/microscope systems.